Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F28 I1 R1	138	62.6	2119710	99.4%	2106991	138.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,637,743	(A)_9→8	coding (1048/1425 nt)	creC →	sensory histidine kinase CreC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,637,735	0	A	.	100.0%	186.2 / NA	43	coding (1040/1425 nt)	creC	sensory histidine kinase CreC
Reads supporting (aligned to +/- strand): ref base A (0/0); new base . (27/16); total (27/16)

TGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTTGCATCACGCTAAG  >  NC_000913/4637606‑4637870

tGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGaaaaaaaatca                                                                                                                               >  2:886704/1‑137 (MQ=255)

       aTTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCa                                                                                                                       >  1:540944/1‑140 (MQ=255)

          ggTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGt                                                                                                                    >  1:429008/1‑140 (MQ=255)

              gAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACg                                                                                                                <  1:263620/140‑1 (MQ=255)

                aaCGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGcc                                                                                                              <  1:454386/140‑1 (MQ=255)

                   gTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCAc                                                                                                           >  1:1057206/1‑140 (MQ=255)

                   gTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCAc                                                                                                           >  1:192260/1‑140 (MQ=255)

                    ttACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCAcc                                                                                                          <  2:303044/140‑1 (MQ=255)

                     tactacGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCg                                                                                                         >  2:276109/1‑140 (MQ=255)

                          cGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGtt                                                                                                    <  2:749549/140‑1 (MQ=255)

                             cAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCCGAAAAAAAAA‑CACTCTGCATGTTACGCCCACCGAGGTTAAc                                                                                                 >  2:520752/1‑140 (MQ=255)

                             cAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAAc                                                                                                 >  1:1001763/1‑140 (MQ=255)

                               ggCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGTGGTTAACGt                                                                                               >  2:525611/1‑140 (MQ=255)

                               ggCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGt                                                                                               <  1:148617/140‑1 (MQ=255)

                                     aCTGGAGAATCGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTtgctgc                                                                                         >  1:824231/1‑140 (MQ=255)

                                         gagaATCGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGaa                                                                                     >  1:541093/1‑140 (MQ=255)

                                           gaATCGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAAcc                                                                                   >  2:447819/1‑140 (MQ=255)

                                             aTCGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCgg                                                                                 <  2:192260/140‑1 (MQ=255)

                                              gcatCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCCTTATTCCCCCGCCTCACCCGACCGCGCCCCGTGCAGGTGGCA‑ACAAAAAAAGAACCCTGCTCGTTACGCCCGCCGAGGTTAACGGTGCTGCTGAACCGGc                                                                                >  2:607847/4‑140 (MQ=255)

                                                   aGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTAc                                                                           >  1:822470/1‑140 (MQ=255)

                                                   aGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTAc                                                                           >  2:231795/1‑140 (MQ=255)

                                                        gTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAg                                                                      >  1:447828/1‑140 (MQ=255)

                                                        gTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAg                                                                      >  2:1005026/1‑140 (MQ=255)

                                                        gTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAg                                                                      >  2:190626/1‑140 (MQ=255)

                                                                gACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCt                                                              <  2:1001763/140‑1 (MQ=255)

                                                                   tgctgTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTggg                                                           >  2:927534/1‑140 (MQ=255)

                                                                      tgTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCAGCGTTACTGGAGCAGGCGCTGGGaaa                                                        >  2:939992/1‑140 (MQ=255)

                                                                        ttGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAAtt                                                      <  2:227549/140‑1 (MQ=255)

                                                                          gATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTa                                                    <  1:1005026/140‑1 (MQ=255)

                                                                          gATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTa                                                    <  2:541093/140‑1 (MQ=255)

                                                                           aTGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTAc                                                   >  2:279241/1‑140 (MQ=255)

                                                                               ggcggcATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTACTGGa                                               >  1:756447/1‑140 (MQ=255)

                                                                                gcggcATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTACTGGAt                                              >  2:828915/1‑140 (MQ=255)

                                                                                        attCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTACTGGATAACGCCAt                                      <  1:711182/140‑1 (MQ=255)

                                                                                        attCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTACTGGATAACGCCAt                                      <  1:231795/140‑1 (MQ=255)

                                                                                                 cgTCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTACTGGATAACGCCATCGATTTTAc                             >  1:534519/1‑140 (MQ=255)

                                                                                                   tCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTACTGGATAACGCCATCGATTTTAccc                           >  1:613913/1‑140 (MQ=255)

                                                                                                   tCAGCGAAGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTACTGGATAACGCCATCGATTTTAccc                           >  2:914677/1‑140 (MQ=255)

                                                                                                          aGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTACTGGATAACGCCATCGATTTTACCCCCGagag                    <  2:61728/140‑1 (MQ=255)

                                                                                                          aGCGCGCACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTACTGGATAACGCCATCGATTTTACCCCCGagag                    <  2:1057206/140‑1 (MQ=255)

                                                                                                           gcgcgcACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTACTGGATAACGCCATc                                     >  1:621440/1‑122 (MQ=255)

                                                                                                           gcgcgcACCGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTACTGGATAACGCCATc                                     <  2:621440/122‑1 (MQ=255)

                                                                                                                  ccGTGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTTGCa            <  1:939992/140‑1 (MQ=255)

                                                                                                                     tGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTTGCATCa         >  1:962893/1‑140 (MQ=255)

                                                                                                                     tGCAGTTGGCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTTGCATCa         >  1:333947/1‑140 (MQ=255)

                                                                                                                            ggCAG‑AAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTTGCATCACGCTAAg  >  1:461310/1‑140 (MQ=255)

TGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTTGCATCACGCTAAG  >  NC_000913/4637606‑4637870

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A