Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F28 I1 R1	126	72.5	2472046	99.4%	2457213	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,302,258	(T)_5→4	coding (821/2052 nt)	mdtO ←	putative multidrug efflux pump subunit MdtO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,302,254	0	T	.	97.5%	333.3 / 2.7	81	coding (825/2052 nt)	mdtO	putative multidrug efflux pump subunit MdtO
Reads supporting (aligned to +/- strand): ref base T (1/1); new base . (37/42); total (38/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.39e-01

GTTGTAACAACGTCTGGCAGATATTCTCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGAGTAAATGTAGGTTACCGTTGCCACGCAGCTTTGCCACCATGCGTTTTGAGTTCGCCAGT  >  NC_000913/4302115‑4302381

gTTGTAACAACGTCTGGCAGATATTCTCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAAt                                                                                                                                 >  2:348505/1‑140 (MQ=255)

 ttGTAACAACGTCTGGCAGATATTCTCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAAtt                                                                                                                                >  2:1184638/1‑140 (MQ=255)

     aacaacGTCTGGCAGATATTCTCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAAttttgt                                                                                                                            <  1:671620/140‑3 (MQ=255)

     aacaacGTCTGGCAGATATTCTCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAAttttgt                                                                                                                            >  1:1103873/1‑138 (MQ=255)

     aacaacGTCTGGCAGATATTCTCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAAttttgt                                                                                                                            >  2:121010/1‑138 (MQ=255)

     aacaacGTCTGGCAGATATTCTCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAAttttgt                                                                                                                            <  1:400986/140‑3 (MQ=255)

       caacGTCTGGCAGATATTCTCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAAttttgtcg                                                                                                                          <  2:857043/140‑5 (MQ=255)

       caacGTCTGGCAGATATTCTCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAAttttgtcg                                                                                                                          <  2:202283/140‑5 (MQ=255)

        aacGTCTGGCAGATATTCTCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAA‑TTTTGTCgg                                                                                                                        <  1:62033/140‑1 (MQ=255)

        aacGTCTGGCAGATATTCTCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAA‑TTTTGTCgg                                                                                                                        <  2:181669/140‑1 (MQ=255)

          cGTCTGGCAGATATTCTCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAA‑TTTTGTCGGaa                                                                                                                      >  1:95612/1‑140 (MQ=255)

          cGTCTGGCAGATATTCTCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAA‑TTTTGTCGGaa                                                                                                                      >  1:598386/1‑140 (MQ=255)

          cGTCTGGCAGATATTCTCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAA‑TTTTGTCGGaa                                                                                                                      <  2:598598/140‑1 (MQ=255)

           gTCTGGCAGATATTCTCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAAttttgt                                                                                                                            <  2:105986/134‑3 (MQ=255)

           gTCTGGCAGATATTCTCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAAttttgt                                                                                                                            >  1:105986/1‑132 (MQ=255)

           gTCTGGCAGATATTCTCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAA‑TTTTGTCGGAAt                                                                                                                     >  1:156105/1‑140 (MQ=255)

           gTCTGGCAGATATTCTCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAA‑TTTTGTCGGAAt                                                                                                                     >  1:583554/1‑140 (MQ=255)

           gTCTGGCAGATATTCTCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAA‑TTTTGTCGGAAt                                                                                                                     >  1:1184480/1‑140 (MQ=255)

           gTCTGGCAGATATTCTCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAA‑TTTTGTCGGAAt                                                                                                                     >  1:328626/1‑140 (MQ=255)

            tCTGGCAGATATTCTCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAA‑TTTTGTCGGAAtt                                                                                                                    >  1:834868/1‑140 (MQ=255)

                  aGATATTCTCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAA‑TTTTGTCGGAATTCaataa                                                                                                              <  1:1079448/140‑1 (MQ=255)

                  aGATATCCTCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAA‑TTTTGTCGGAATTCaataa                                                                                                              <  2:703715/140‑1 (MQ=255)

                    atatTCTCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAA‑TTTTGTCGGAATTCAATAAtt                                                                                                            >  1:269111/1‑140 (MQ=255)

                     tatTCTCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAA‑TTTTGTCGGAATTCaataat                                                                                                             >  2:1067760/1‑138 (MQ=255)

                     tatTCTCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAA‑TTTTGTCGGAATTCaataat                                                                                                             <  1:1067760/138‑1 (MQ=255)

                     tatTCTCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAA‑TTTTGTCGGAATTCaataa                                                                                                              >  1:1206241/1‑137 (MQ=255)

                     tatTCTCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAA‑TTTTGTCGGAATTCaataa                                                                                                              <  2:1206241/137‑1 (MQ=255)

                      atTCTCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAA‑TTTTGTCGGAATTCAATAATTGc                                                                                                          >  2:67223/1‑140 (MQ=255)

                      atTCTCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTAGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAA‑TTTTGTCGGAATTCAATAATTGc                                                                                                          >  2:1102334/1‑140 (MQ=255)

                            cAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAA‑TTTTGTCGGAATTCAATAATTGCCTgaga                                                                                                    <  2:231899/140‑1 (MQ=255)

                               gTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAA‑TTTTGTCGGAATTCAATAATTGCCTGAGAATc                                                                                                 >  2:886242/1‑140 (MQ=255)

                                  aCATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAA‑TTTTGTCGGAATTCAATAATTGCCTGAGAATCAGc                                                                                              >  1:902776/1‑140 (MQ=255)

                                   cATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAA‑TTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCa                                                                                             >  2:1214854/1‑140 (MQ=255)

                                    aTTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAA‑TTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCaa                                                                                            <  1:571851/140‑1 (MQ=255)

                                      tCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAA‑TTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCaaaa                                                                                          <  1:67223/140‑1 (MQ=255)

                                       cccGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAA‑TTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAag                                                                                         >  1:261270/1‑140 (MQ=255)

                                        ccGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAA‑TTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAaga                                                                                        <  2:261270/140‑1 (MQ=255)

                                         cGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAA‑TTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAagag                                                                                       >  1:1118858/1‑140 (MQ=255)

                                          gTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAA‑TTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAgg                                                                                      <  2:902776/140‑1 (MQ=255)

                                           tGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAA‑TTTTGTCGGAATTCAATAATTGCCTgaga                                                                                                    <  2:707474/125‑1 (MQ=255)

                                           tGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAA‑TTTTGTCGGAATTCAATAATTGCCTgaga                                                                                                    >  1:707474/1‑125 (MQ=255)

                                           tGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAA‑TTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGt                                                                                     <  1:291099/140‑1 (MQ=255)

                                           tGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAA‑TTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGt                                                                                     >  2:442031/1‑140 (MQ=255)

                                              cgccATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAA‑TTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTggg                                                                                  >  2:469599/1‑140 (MQ=255)

                                                ccATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAA‑TTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGAt                                                                                >  2:424103/1‑140 (MQ=255)

                                                ccATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAA‑TTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGAt                                                                                <  2:95612/140‑1 (MQ=255)

                                                     gCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAA‑TTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAg                                                                           <  2:451493/140‑1 (MQ=255)

                                                        tcactttcactGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAA‑TTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGa                                                                        >  1:453780/1‑140 (MQ=255)

                                                          actttcactGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAA‑TTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGAtt                                                                      >  1:831001/1‑140 (MQ=255)

                                                               cactGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAA‑TTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCg                                                                 <  2:831001/140‑1 (MQ=255)

                                                               cactGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAA‑TTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCg                                                                 <  1:442031/140‑1 (MQ=255)

                                                               cactGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAA‑TTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCg                                                                 <  2:269111/140‑1 (MQ=255)

                                                                actGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAA‑TTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTTGCGATTCAGCGt                                                                <  2:1118858/140‑1 (MQ=255)

                                                                 ctGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAATTTT‑‑TCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCg                                                                 <  2:253093/137‑1 (MQ=255)

                                                                 ctGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAATTTT‑‑TCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCg                                                                 >  1:253093/1‑137 (MQ=255)

                                                                   gATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAA‑TTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGa                                                             <  2:453780/140‑1 (MQ=255)

                                                                    aTCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAA‑TTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGt                                                                >  2:206436/1‑136 (MQ=255)

                                                                    aTCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAA‑TTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGt                                                                <  1:206436/136‑1 (MQ=255)

                                                                    aTCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAA‑TTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGAg                                                            <  2:1122618/140‑1 (MQ=255)

                                                                    aTCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAA‑TTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGAg                                                            <  1:1214854/140‑1 (MQ=255)

                                                                     tCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAA‑TTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGAGt                                                           <  2:156105/140‑1 (MQ=255)

                                                                     tCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAA‑TTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGAGt                                                           <  2:583554/140‑1 (MQ=255)

                                                                     tCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAA‑TTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGAGt                                                           <  2:593231/140‑1 (MQ=255)

                                                                      ccGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAA‑TTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGAGTa                                                          >  2:576590/1‑140 (MQ=255)

                                                                         ccAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAA‑TTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGAGTAAAt                                                       <  1:1102334/140‑1 (MQ=255)

                                                                         ccAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAA‑TTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGAGTAAAt                                                       >  2:200293/1‑140 (MQ=255)

                                                                              cGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAA‑TTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGAg                                                            <  1:363217/130‑1 (MQ=255)

                                                                              cGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAA‑TTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGAg                                                            >  2:363217/1‑130 (MQ=255)

                                                                              cGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAA‑TTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGAGTAAATGTAgg                                                  >  1:476522/1‑140 (MQ=255)

                                                                               gCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAA‑TTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGAGTAAATGTAGGt                                                 <  1:121010/140‑1 (MQ=255)

                                                                                     ccAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAA‑TTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGAGTAAATGTAGGTTACCGt                                           <  1:273576/140‑1 (MQ=255)

                                                                                     ccAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAA‑TTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGAGTAAATGTAGGTTACCGt                                           <  2:125176/140‑1 (MQ=255)

                                                                                       aGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAA‑TTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGAGTAAATGTAGGTTACCGTTg                                         <  1:886242/140‑1 (MQ=255)

                                                                                        gCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAA‑TTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGAGTAAATGTAGGTTACCGTTGc                                        <  1:1184638/140‑1 (MQ=255)

                                                                                          aCTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAA‑TTTTGTCGGAATTCAATAATTGCCTGAGAATCGGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGAGTAAATGTAGGTTACCGTTGCCa                                      <  2:301853/140‑1 (MQ=255)

                                                                                           cTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAA‑TTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGAGTAAATGTAGGTTACCGTTGCCAc                                     >  1:1178979/1‑140 (MQ=255)

                                                                                            tGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAA‑TTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGAGTAAATGTAGGTTACCGTTGCCACg                                    <  2:328626/140‑1 (MQ=255)

                                                                                             gACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAA‑TTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGAGTAAATGTAGGTTACCGTTGCCACGc                                   <  1:348505/140‑1 (MQ=255)

                                                                                             gACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAA‑TTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGAGTAAATGTAGGTTACCGTTGCCACGc                                   <  1:576590/140‑1 (MQ=255)

                                                                                                   cAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAA‑TTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGAGTAAATGTAGGTTACCGTTGCCACGCAGCttt                             >  2:199028/1‑140 (MQ=255)

                                                                                                        aCGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAA‑TTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGAGTAAATGTAGGTTACCGTTGCCACGCAg                                 >  1:630601/1‑131 (MQ=255)

                                                                                                        aCGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAA‑TTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGAGTAAATGTAGGTTACCGTTGCCACGCAg                                 <  2:630601/131‑1 (MQ=255)

                                                                                                        aCGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAA‑TTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGAGTAAATGTAGGTTACCGTTGCCACGCAGCTTTGccac                        >  2:161168/1‑140 (MQ=255)

                                                                                                         cGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAA‑TTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGAGTAAATGTAGGTTACCGTTGCCACGCAGCTTTGccacc                       >  2:1040248/1‑140 (MQ=255)

                                                                                                          ggCATGCTGCAGCTTGTTGATTTCTGAAGCTAA‑TTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGAGTAAATGTAGGTTACCGTTGCCACGCAGCTTTGccacca                      >  1:817757/1‑140 (MQ=255)

                                                                                                              tgctgcAGCTTGTTGATTTCTGAAGCTAA‑TTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGAGTAAATGTAGGTTACCGTTGCCACGCAGCTTTGCCACCATGCg                  >  2:1009459/1‑140 (MQ=255)

                                                                                                                ctgcAGCTTGTTGATTTCTGAAGCTAA‑TTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGAGTAAATGTAGGTTACCGTTGCCACGCAGCTTTGCCACCATGCGtt                >  1:172744/1‑140 (MQ=255)

                                                                                                                     gCTTGTTGATTTCTGAAGCTAA‑TTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGAGTAAATGTAGGTTACCGTTGCCACGCAGCTTTGCCACCATGCGTTTTGAg           <  1:161168/140‑1 (MQ=255)

                                                                                                                         gttgATTTCTGAAGCTAA‑TTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGAGTAAATGTAGGTTACCGTTGCCACGCAGCTTTGCCACCATGCGTTTTGAGTTCg       <  1:1108347/140‑1 (MQ=255)

                                                                                                                          ttgATTTCTGAAGCTAA‑TTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGAGTAAATGTAGGTTACCGTTGCCACGCAGCTTTGCCACCATGCGTTTTGAGTTCGc      >  1:374568/1‑140 (MQ=255)

                                                                                                                              tttCTGAAGCTAA‑TTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGAGTAAATGTAGGTTACCGTTGCCACGCAGCTTTGCCACCATGCGTTTTGAGTTCGCCAGt  <  1:998304/140‑1 (MQ=255)

GTTGTAACAACGTCTGGCAGATATTCTCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCAGCAAAAGAGGTGGGATCGTAGCGATTCAGCGTCGAGTAAATGTAGGTTACCGTTGCCACGCAGCTTTGCCACCATGCGTTTTGAGTTCGCCAGT  >  NC_000913/4302115‑4302381

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A