Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A10 F28 I1 R1 126 72.5 2472046 99.4% 2457213 138.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,400,845 A→G V58V (GTA→GTG)  hflX → ribosome rescue factor HflX

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,400,8450AG98.8% 292.5 / ‑4.5 81V58V (GTA→GTG) hflXribosome rescue factor HflX
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (43/37);  total (44/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.41e-01

TGGTACACATCTATTTTACGCAAGACAAAGATATGGAAGACCTCCAGGAGTTTGAATCTCTGGTCTCTTCCGCCGGTGTCGAAGCATTGCAGGTGATTACCGGTAGCCGTAAAGCGCCGCACCCAAAGTATTTTGTAGGTGAAGGTAAAGCAGTTGAAATTGCGGAAGCTGTCAAAGCGACGGGTGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGCCCGGCGCAAGAGCGTAACCTGGAGCGTTTGTGCGAGTGTCGTGTTATCGACCGCA  >  NC_000913/4400709‑4400981
                                                                                                                                        |                                                                                                                                        
tGGTACACATCTATTTTACGCAAGACAAAGATATGGAAGACCTCCAGGAGTTTGAATCTCTGGTCTCTTCCGCCGGTGTCGAAGCATTGCAGGTGATTACCGGTAGCCGTAAAGCGCCGCACCCAAAGTATTTTGTGGGt                                                                                                                                       <  2:159612/140‑1 (MQ=255)
tGGTACACATCTATTTTACGCAAGACAAAGATATGGAAGACCTCCAGGAGTTTGAATCTCTGGTCTCTTCCGCCGGTGTCGAAGCATTGCAGGTGATTACCGGTAGCCGTAAAGCGCCGCACCCAAAGTATTTTGTGGGt                                                                                                                                       <  2:741204/140‑1 (MQ=255)
tGGTACACATCTATTTTACGCAAGACAAAGATATGGAAGACCTCCAGGAGTTTGAATCTCTGGTCTCTTCCGCCGGTGTCGAAGCATTGCAGGTGATTACCGGTAGCCGTAAAGCGCCGCACCCAAAGTATTTTGTGGGt                                                                                                                                       <  1:926886/140‑1 (MQ=255)
tGGTACACATCTATTTTACGCAAGACAAAGATATGGAAGACCTCCAGGAGTTTGAATCTCTGGTCTCTTCCGCCGGTGTCGAAGCATTGCAGGTGATTACCGGTAGCCGTAAAGCGCCGCACCCAAAGTATTTTGTGGGt                                                                                                                                       <  1:281014/140‑1 (MQ=255)
   tACACATCTATTTTACGCAAGACAAAGATATGGAAGACCTCCAGGAGTTTGAATCTCTGGTCTCTTCCGCCGGTGTCGAAGCATTGCAGGTGATTACCGGTAGCCGTAAAGCGCCGCACCCAAAGTATTTTGTGGGTGaa                                                                                                                                    >  2:783240/1‑140 (MQ=255)
   tACACATCTATTTTACGCAAGACAAAGATATGGAAGACCTCCAGGAGTTTGAATCTCTGGTCTCTTCCGCCGGTGTCGAAGCATTGCAGGTGATTACCGGTAGCCGTAAAGCGCCGCACCCAAAGTATTTTGTGGGTGaa                                                                                                                                    >  1:1214947/1‑140 (MQ=255)
   tACACATCTATTTTACGCAAGACAAAGATATGGAAGACCTCCAGGAGTTTGAATCTCTGGTCTCTTCCGCCGGTGTCGAAGCATTGCAGGTGATTACCGGTAGCCGTAAAGCGCCGCACCCAAAGTATTTTGTGGGTGaa                                                                                                                                    >  1:192442/1‑140 (MQ=255)
   tACACATCTATTTTACGCAAGACAAAGATATGGAAGACCTCCAGGAGTTTGAATCTCTGGTCTCTTCCGCCGGTGTCGAAGCATTGCAGGTGATTACCGGTAGCCGTAAAGCGCCGCACCCAAAGTATTTTGTGGGTGaa                                                                                                                                    >  2:909008/1‑140 (MQ=255)
       caTCTATTTTACGCAAGACAAAGATATGGAAGACCTCCAGGAGTTTGAATCTCTGGTCTCTTCCGCCGGTGTCGAAGCATTGCAGGTGATTACCGGTAGCCGTAAAGCGCCGCACCCAAAGTATTTTGTGGGTGAAGGTa                                                                                                                                >  2:244221/1‑140 (MQ=255)
       caTCTATTTTACGCAAGACAAAGATATGGAAGACCTCCAGGAGTTTGAATCTCTGGTCTCTTCCGCCGGTGTCGAAGCATTGCAGGTGATTACCGGTAGCCGTAAAGCGCCGCACCCAAAGTATTTTGTGGGTGAAGGTa                                                                                                                                >  2:568831/1‑140 (MQ=255)
       caTCTATTTTACGCAAGACAAAGATATGGAAGACCTCCAGGAGTTTGAATCTCTGGTCTCTTCCGCCGGTGTCGAAGCATTGCAGGTGATTACCGGTAGCCGTAAAGCGCCGCACCCAAAGTATTTTGTGGGTGAAGGTa                                                                                                                                >  1:832303/1‑140 (MQ=255)
       caTCTATTTTACGCAAGACAAAGATATGGAAGACCTCCAGGAGTTTGAATCTCTGGTCTCTTCCGCCGGTGTCGAAGCATTGCAGGTGATTACCGGTAGCCGTAAAGCGCCGCACCCAAAGTATTTTGTGGGTGAAGGTa                                                                                                                                <  1:568831/140‑1 (MQ=255)
       caTCTATTTTACGCAAGACAAAGATATGGAAGACCTCCAGGAGTTTGAATCTCTGGTCTCTTCCGCCGGTGTCGAAGCATTGCAGGTGATTACCGGTAGCCGTAAAGCGCCGCACCCAAAGTATTTTGTGGGTGAAGGTa                                                                                                                                <  1:3485/140‑1 (MQ=255)
        aTCTATTTTACGCAAGACAAAGATATGGAAGACCTCCAGGAGTTTGAATCTCTGGTCTCTTCCGCCGGTGTCGAAGCATTGCAGGTGATTACCGGTAGCCGTAAAGCGCCGCACCCAAAGTATTTTGTGGGTGAAGGTaa                                                                                                                               >  2:408511/1‑140 (MQ=255)
          cTATTTTACGCAAGACAAAGATATGGAAGACCTCCAGGAGTTTGAATCTCTGGTCTCTTCCGCCGGTGTCGAAGCATTGCAGGTGATTACCGGTAGCCGTAAAGCGCCGCACCCAAAGTATTTTGTGGGTGAAGGTAAAg                                                                                                                             >  2:344345/1‑140 (MQ=255)
            aTTTTACGCAAGACAAAGATATGGAAGACCTCCAGGAGTTTGAATCTCTGGTCTCTTCCGCCGGTGTCGAAGCATTGCAGGTGATTACCGGTAGCCGTAAAGCGCCGCACCCAAAGTATTTTGTGGGTGAAGGTAAAGCa                                                                                                                           >  2:975683/1‑140 (MQ=255)
                  cGCAAGACAAAGATATGGAAGACCTCCAGGAGTTTGAATCTCTGGTCTCTTCCGCCGGTGTCGAAGCATTGCAGGTGATTACCGGTAGCCGTAAAGCGCCGCACCCAAAGTATTTTGTGGGTGAAGGTAAAGCAGTTGaa                                                                                                                     >  1:118246/1‑140 (MQ=255)
                       gACAAAGATATGGAAGACCTCCAGGAGTTTGAATCTCTGGTCTCTTCCGCCGGTGTCGAAGCATTGCAGGTGATTACCGGTAGCCGTAAAGCGCCGCACCCAAAGTATTTTGTGGGTGAAGGTAAAGCAGTTGAAATTGc                                                                                                                >  2:1004912/1‑140 (MQ=255)
                                 tGGAAGACCTCCAGGAGTTTGAATCTCTGGTCTCTTCCGCCGGTGTCGAAGCATTGCAGGTGATTACCGGTAGCCGTAAAGCGCCGCACCCAAAGTATTTTGTGGGTGAAGGTAAAGCAGTTGAAATTGCGGa                                                                                                             <  1:490829/133‑1 (MQ=255)
                                 tGGAAGACCTCCAGGAGTTTGAATCTCTGGTCTCTTCCGCCGGTGTCGAAGCATTGCAGGTGATTACCGGTAGCCGTAAAGCGCCGCACCCAAAGTATTTTGTGGGTGAAGGTAAAGCAGTTGAAATTGCGGa                                                                                                             >  2:490829/1‑133 (MQ=255)
                                 tGGAAGACCTCCAGGAGTTTGAATCTCTGGTCTCTTCCGCCGGTGTCGAAGCATTGCAGGTGATTACCGGTAGCCGTAAAGCGCCGCACCCAAAGTATTTTGTGGGTGAAGGTAAAGCAGTTGAAATTGCGGAAGCTGTc                                                                                                      >  1:582008/1‑140 (MQ=255)
                                 tGGAAGACCTCCAGGAGTTTGAATCTCTGGTCTCTTCCGCCGGTGTCGAAGCATTGCAGGTGATTACCGGTAGCCGTAAAGCGCCGCACCCAAAGTATTTTGTGGGTGAAGGTAAAGCAGTTGAAATTGCGGAAGCTGTc                                                                                                      <  1:767220/140‑1 (MQ=255)
                                         cTCCAGGAGTTTGAATCTCTGGTCTCTTCCGCCGGTGTCGAAGCATTGCAGGTGATTACCGGTAGCCGTAAAGCGCCGCACCCAAAGTATTTTGTGGGTGAAGGTAAAGCAGTTGAAATTGCGGAAGCTGTCAAAGCGAc                                                                                              >  1:574896/1‑140 (MQ=255)
                                          tCCAGGAGTTTGAATCTCTGGTCTCTTCCGCCGGTGTCGAAGCATTGCAGGTGATTACCGGTAGCCGTAAAGCGCCGCACCCAAAGTATTTTGTGGGTGAAGGTAAAGCAGTTGAAATTGCGGAAGCTGTCAAAGCGACg                                                                                             >  2:940971/1‑140 (MQ=255)
                                           ccAGGAGTTTGAATCTCTGGTCTCTTCCGCCGGTGTCGAAGCATTGCAGGTGATTACCGGTAGCCGTAAAGCGCCGCACCCAAAGTATTTTGTAGGTGAAGGTAAAGCAGTTGAAATTGCGGAAGCTGTCAAAGCGACgg                                                                                            >  1:283844/1‑140 (MQ=255)
                                            cAGGAGTTTGAATCTCTGGTCTCTTCCGCCGGTGTCGAAGCATTGCAGGTGATTACCGGTAGCCGTAAAGCGCCGCACCCAAAGTATTTTGTGGGTGAAGGTAAAGCAGTTGAAATTGCGGAAGCTGTCAAAGCGACggg                                                                                           <  1:408511/140‑1 (MQ=255)
                                                aGTTTGAATCTCTGGTCTCTTCCGCCGGTGTCGAAGCATTGCAGGTGATTACCGGTAGCCGTAAAGCGCCGCACCCAAAGTATTTTGTGGGTGAAGGTAAAGCAGTTGAAATTGCGGAAGCTGTCAAAGCGACGGGTGCt                                                                                       <  1:909008/140‑1 (MQ=255)
                                                  tttGAATCTCTGGTCTCTTCCGCCGGTGTCGAAGCATTGCAGGTGATTACCGGTAGCCGTAAAGCGCCGCACCCAAAGTATTTTGTGGGTGAAGGTAAAGCAGTTGAAATTGCGGAAGCTGTCAAAGCGACGGGTGCTTc                                                                                     >  2:638885/1‑140 (MQ=255)
                                                  tttGAATCTCTGGTCTCTTCCGCCGGTGTCGAAGCATTGCAGGTGATTACCGGTAGCCGTAAAGCGCCGCACCCAAAGTATTTTGTGGGTGAAGGTAAAGCAGTTGAAATTGCGGAAGCTGTCAAAGCGACGGGTGCTTc                                                                                     >  1:1139079/1‑140 (MQ=255)
                                                   ttGAATCTCTGGTCTCTTCCGCCGGTGTCGAAGCATTGCAGGTGATTACCGGTAGCCGTAAAGCGCCGCACCCAAAGTATTTTGTGGGTGAAGGTAAAGCAGTTGAAATTGCGGAAGCTGTCAAAGCGACGGGTGCTTCg                                                                                    <  1:1210389/140‑1 (MQ=255)
                                                         ctctGGTCTCTTCCGCCGGTGTCGAAGCATTGCAGGTGATTACCGGTAGCCGTAAAGCGCCGCACCCAAAGTATTTTGTGGGTGAAGGTAAAGCAGTTGAAATTGCGGAAGCTGTCAAAGCGACGGGTGCTTCGGTCGtt                                                                              >  2:340354/1‑140 (MQ=255)
                                                         ctctGGTCTCTTCCGCCGGTGTCGAAGCATTGCAGGTGATTACCGGTAGCCGTAAAGCGCCGCACCCAAAGTATTTTGTGGGTGAAGGTAAAGCAGTTGAAATTGCGGAAGCTGTCAAAGCGACAGGTGCTTCGGTCGtt                                                                              >  2:842571/1‑140 (MQ=255)
                                                           ctGGTCTCTTCCGCCGGTGTCGAAGCATTGCAGGTGATTACCGGTAGCCGTAAAGCGCCGCACCCAAAGTATTTTGTGGGTGAAGGTAAAGCAGTTGAAATTGCGGAAGCTGTCAAAGCGACGGGTGCTTCGGTCGTTCt                                                                            >  1:1132123/1‑140 (MQ=255)
                                                              gTCTCTTCCGCCGGTGTCGAAGCATTGCAGGTGATTACCGGTAGCCGTAAAGCGCCGCACCCAAAGTATTTTGTGGGTGAAGGTAAAGCAGTTGAAATTGCGGAAGCTGTCAAAGCGACGGGTGCTTCGGTCGTTCtttt                                                                         <  1:975683/140‑1 (MQ=255)
                                                                ctctTCCGCCGGTGTCGAAGCATTGCAGGTGATTACCGGTAGCCGTAAAGCGCCGCACCCAAAGTATTTTGTGGGTGAAGGTAAAGCAGTTGAAATTGCGGAAGCTGTCAAAGCGACGGGTGCTTCGGTCGTTCTTTTTg                                                                       >  2:790242/1‑140 (MQ=255)
                                                                ctctTCCGCCGGTGTCGAAGCATTGCAGGTGATTACCGGTAGCCGTAAAGCGCCGCACCCAAAGTATTTTGTGGGTGAAGGTAAAGCAGTTGAAATTGCGGAAGCTGTCAAAGCGACGGGTGCTTCGGTCGTTCTTTTTg                                                                       <  2:1139079/140‑1 (MQ=255)
                                                                 tctTCCGCCGGTGTCGAAGCATTGCAGGTGATTACCGGTAGCCGTAAAGCGCCGCACCCAAAGTATTTTGTGGGTGAAGGTAAAGCAGTTGAAATTGCGGAAGCTGTCAAAGCGACGGGTGCTTCGGTCGTTCTTTTTGa                                                                      >  1:911946/1‑140 (MQ=255)
                                                                   ttCCGCCGGTGTCGAAGCATTGCAGGTGATTACCGGTAGCCGTAAAGCGCCGCACCCAAAGTATTTTGTGGGTGAAGGTAAAGCAGTTGAAATTGCGGAAGCTGTCAAAGCGACGGGTGCTTCGGTCGTTCTTTTTGAcc                                                                    <  2:70675/140‑1 (MQ=255)
                                                                   ttCCGCCGGTGTCGAAGCATTGCAGGTGATTACCGGTAGCCGTAAAGCGCCGCACCCAAAGTATTTTGTGGGTGAAGGTAAAGCAGTTGAAATTGCGGAAGCTGTCAAAGCGACGGGTGCTTCGGTCGTTCTTTTTGAcc                                                                    <  1:638885/140‑1 (MQ=255)
                                                                    tCCGCCGGTGTCGAAGCATTGCAGGTGATTACCGGTAGCCGTAAAGCGCCGCACCCAAAGTATTTTGTGGGTGAAGGTAAAGCAGTTGAAATTGCGGAAGCTGTCAAAGCGACGGGTGCTTCGGTCGTTCTTTTTGACCa                                                                   <  2:716449/140‑1 (MQ=255)
                                                                        ccgGTGTCGAAGCATTGCAGGTGATTACCGGTAGCCGTAAAGCGCCGCACCCAAAGTATTTTGTGGGTGAAGGTAAAGCAGTTGAAATTGCGGAAGCTGTCAAAGCGACGGGTGCTTCGGTCGTTCTTTTTGAc                                                                     <  2:1185701/134‑1 (MQ=255)
                                                                        ccgGTGTCGAAGCATTGCAGGTGATTACCGGTAGCCGTAAAGCGCCGCACCCAAAGTATTTTGTGGGTGAAGGTAAAGCAGTTGAAATTGCGGAAGCTGTCAAAGCGACGGGTGCTTCGGTCGTTCTTTTTGAc                                                                     >  1:1185701/1‑134 (MQ=255)
                                                                        ccgGTGTCGAAGCATTGCAGGTGATTACCGGTAGCCGTAAAGCGCCGCACCCAAAGTATTTTGTGGGTGAAGGTAAAGCAGTTGAAATTGCGGAAGCTGTCAAAGCGACGGGTGCTTCGGTCGTTCTTTTTGACCATGcc                                                               >  2:766801/1‑140 (MQ=255)
                                                                        ccgGTGTCGAAGCATTGCAGGTGATTACCGGTAGCCGTAAAGCGCCGCACCCAAAGTATTTTGTGGGTGAAGGTAAAGCAGTTGAAATTGCGGAAGCTGTCAAAGCGACGGGTGCTTCGGTCGTTCTTTTTGACCATGcc                                                               >  2:1151312/1‑140 (MQ=255)
                                                                        ccgGTGTCGAAGCATTGCAGGTGATTACCGGTAGCCGTAAAGCGCCGCACCCAAAGTATTTTGTGGGTGAAGGTAAAGCAGTTGAAATTGCGGAAGCTGTCAAAGCGACGGGTGCTTCGGTCGTTCTTTTTGACCATGcc                                                               >  1:675981/1‑140 (MQ=255)
                                                                             gtCGAAGCATTGCAGGTGATTACCGGTAGCCGTAAAGCGCCGCACCCAAAGTATTTTGTGGGTGAAGGTAAAGCAGTTGAAATTGCGGAAGCTGTCAAAGCGACGGGTGCTTCGGTCGTTCTTTTTGACCATGCCCTGAg                                                          >  1:713473/1‑140 (MQ=255)
                                                                               cGAAGCATTGCAGGTGATTACCGGTAGCCGTAAAGCGCCGCACCCAAAGTATTTTGTGGGTGAAGGTAAAGCAGTTGAAATTGCGGAAGCTGTCAAAGCGACGGGTGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGcc                                                        <  1:1151312/140‑1 (MQ=255)
                                                                               cGAAGCATTGCAGGTGATTACCGGTAGCCGTAAAGCGCCGCACCCAAAGTATTTTGTGGGTGAAGGTAAAGCAGTTGAAATTGCGGAAGCTGTCAAAGCGACGGGTGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGcc                                                        <  1:244221/140‑1 (MQ=255)
                                                                               cGAAGCATTGCAGGTGATTACCGGTAGCCGTAAAGCGCCGCACCCAAAGTATTTTGTGGGTGAAGGTAAAGCAGTTGAAATTGCGGAAGCTGTCAAAGCGACGGGTGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGcc                                                        <  2:1132123/140‑1 (MQ=255)
                                                                                    cATTGCAGGTGATTACCGGTAGCCGTAAAGCGCCGCACCCAAAGTATTTTGTGGGTGAAGGTAAAGCAGTTGAAATTGCGGAAGCTGTCAAAGCGACGGGTGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGCCCGgcg                                                   >  2:1221164/1‑140 (MQ=255)
                                                                                      ttGCAGGTGATTACCGGTAGCCGTAAAGCGCCGCACCCAAAGTATTTTGTGGGTGAAGGTAAAGCAGTTGAAATTGCGGAAGCTGTCAAAGCGACGGGTGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGCCCGGCGCa                                                 <  2:118246/140‑1 (MQ=255)
                                                                                      ttGCAGGTGATTACCGGTAGCCGTAAAGCGCCGCAACCAAAGTATTTTGTGGGTGAAGGTAAAGCAGTTGAAATTGCGGAAGCTGTCAAAGCGACGGGTGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGCCCGGCGCa                                                 <  2:853509/140‑1 (MQ=255)
                                                                                       tGCAGGTGATTACCGGTAGCCGTAAAGCGCCGCACCCAAAGTATTTTGTGGGTGAAGGTAAAGCAGTTGAAATTGCGGAAGCTGTCAAAGCGACGGGTGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGCCCGGCGCaa                                                >  2:159627/1‑140 (MQ=255)
                                                                                       tGCAGGTGATTACCGGTAGCCGTAAAGCGCCGCACCCAAAGTATTTTGTGGGTGAAGGTAAAGCAGTTGAAATTGCGGAAGCTGTCAAAGCGACGGGTGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGCCCGGCGCaa                                                <  2:970095/140‑1 (MQ=255)
                                                                                           ggTGATTACCGGTAGCCGTAAAGCGCCGCACCCAAAGTATTTTGTGGGTGAAGGTAAAGCAGTTGAAATTGCGGAAGCTGTCAAAGCGACGGGTGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGCCCGGCGCAAGAGc                                            <  2:911946/140‑1 (MQ=255)
                                                                                             tGATTACCGGTAGCCGTAAAGCGCCGCACCCAAAGTATTTTGTGGGTGAAGGTAAAGCAGTTGAAATTGCGGAAGCTGTCAAAGCGACGGGTGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGCCCGGCGCAAGAGCGt                                          <  1:940971/140‑1 (MQ=255)
                                                                                              gATTACCGGTAGCCGTAAAGCGCCGCACCCAAAGTATTTTGTGGGTGAAGGTAAAGCAGTTGAAATTGCGGAAGCTGTCAAAGCGACGGGTGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGCCCGGCGCAAGAGCGTa                                         >  1:1216192/1‑140 (MQ=255)
                                                                                                   ccGGTAGCCGTAAAGCGCCGCACCCAAAGTATTTTGTGGGTGAAGGTAAAGCAGTTGAAATTGCGGAAGCTGTCAAAGCGACGGGTGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGCCCGGCGCAAGAGCGTAACCTg                                    <  2:1083443/140‑1 (MQ=255)
                                                                                                    cGGTAGCCGTAAAGCGCCGCACCCAAAGTATTTTGTGGGTGAAGGTAAAGCAGTTGAAATTGCGGAAGCTGTCAAAGCGACGGGTGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGCCCGGCGCAAGAGCGTAACCTgg                                   >  1:1025393/1‑140 (MQ=255)
                                                                                                    cGGTAGCCGTAAAGCGCCGCACCCAAAGTATTTTGTGGGTGAAGGTAAAGCAGTTGAAATTGCGGAAGCTGTCAAAGCGACGGGTGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGCCCGGCGCAAGAGCGTAACCTgg                                   >  1:334125/1‑140 (MQ=255)
                                                                                                     ggTAGCCGTAAAGCGCCGCACCCAAAGTATTTTGTGGGTGAAGGTAAAGCAGTTGAAATTGCGGAAGCTGTCAAAGCGACGGGTGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGCCCGGCGCAAGAGCGTAACCTGGa                                  <  2:582008/140‑1 (MQ=255)
                                                                                                         gCCGTAAAGCGCCGCACCCAAAGTATTTTGTGGGTGAAGGTAAAGCAGTTGAAATTGCGGAAGCTGTCAAAGCGACGGGTGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGCCCGGCGCAAGAGCGTAACCTGGAGCGt                              >  2:87072/1‑140 (MQ=255)
                                                                                                         gCCGTAAAGCGCCGCACCCAAAGTATTTTGTGGGTGAAGGTAAAGCAGTTGAAATTGCGGAAGCTGTCAAAGCGACGGGTGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGCCCGGCGCAAGAGCGTAACCTGGAGCGt                              >  1:649302/1‑140 (MQ=255)
                                                                                                         gCCGTAAAGCGCCGCACCCAAAGTATTTTGTGGGTGAAGGTAAAGCAGTTGAAATTGCGGAAGCTGTCAAAGCGACGGGTGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGCCCGGCGCAAGAGCGTAACCTGGAGCGt                              >  2:1139237/1‑140 (MQ=255)
                                                                                                          ccGTAAAGCGCCGCACCCAAAGTATTTTGTGGGTGAAGGTAAAGCAGTTGAAATTGCGGAAGCTGTCAAAGCGACGGGTGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGCCCGGCGCAAGAGCGTAACCTGGa                                  >  2:543512/1‑135 (MQ=255)
                                                                                                          ccGTAAAGCGCCGCACCCAAAGTATTTTGTGGGTGAAGGTAAAGCAGTTGAAATTGCGGAAGCTGTCAAAGCGACGGGTGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGCCCGGCGCAAGAGCGTAACCTGGa                                  <  1:543512/135‑1 (MQ=255)
                                                                                                          ccGTAAAGCGCCGCACCCAAAGTATTTTGTGGGTGAAGGTAAAGCAGTTGAAATTGCGGAAGCTGTCAAAGCGACGGGTGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGCCCGGCGCAAGAGCGTAACCTGGAGCGtt                             >  2:1069151/1‑140 (MQ=255)
                                                                                                          ccGTAAAGCGCCGCACCCAAAGTATTTTGTGGGTGAAGGTAAAGCAGTTGAAATTGCGGAAGCTGTCAAAGCGACGGGTGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGCCCGGCGCAAGAGCGTAACCTGGAGCGtt                             <  2:675981/140‑1 (MQ=255)
                                                                                                              aaaGCGCCGCACCCAAAGTATTTTGTGGGTGAAGGTAAAGCAGTTGAAATTGCGGAAGCTGTCAAAGCGACGGGTGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGCCCGGCGCAAGAGCGTAACCTGGAGCGTTtgtg                         <  2:192442/140‑1 (MQ=255)
                                                                                                                 gcgcCGCACCCAAAGTATTTTGTGGGTGAAGGTAAAGCAGTTGAAATTGCGGAAGCTGTCAAAGCGACGGGTGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGCCCGGCGCAAGAGCGTAACCTGGAGCGTTTGTGCGa                      >  1:213583/1‑140 (MQ=255)
                                                                                                                    ccgcACCCAAAGTATTTTGTGGGTGAAGGTAAAGCAGTTGAAATTGCGGAAGCTGTCAAAGCGACGGGTGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGCCCGGCGCAAGAGCGTAACCTGGAGCGTTTGTGCGAgtg                   <  1:1004912/140‑1 (MQ=255)
                                                                                                                    ccgcACCCAAAGTATTTTGTGGGTGAAGGTAAAGCAGTTGAAATTGCGGAAGCTGTCAAAGCGACGGGTGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGCCCGGCGCAAGAGCGTAACCTGGAGCGTTTGTGCGAgtg                   >  2:13721/1‑140 (MQ=255)
                                                                                                                    ccgcACCCAAAGTATTTTGTGGGTGAAGGTAAAGCAGTTGAAATTGCGGAAGCTGTCAAAGCGACGGGTGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGCCCGGCGCAAGAGCGTAACCTGGAGCGTTTGTGCGAgtg                   <  1:1069151/140‑1 (MQ=255)
                                                                                                                      gcACCCAAAGTATTTTGTGGGTGAAGGTAAAGCAGTTGAAATTGCGGAAGCTGTCAAAGCGACGGGTGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGCCCGGCGCAAGAGCGTAACCTGGAGCGTTTGTGCGAGTGTc                 <  1:790242/140‑1 (MQ=255)
                                                                                                                            aaaGTATTTTGTGGGTGAAGGTAAAGCAGTTGAAATTGCGGAAGCTGTCAAAGCGACGGGTGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGCCCGGCGCAAGAGCGTAACCTGGAGCGTTTGTGCGAGTGTCGTGTTa           <  2:713473/140‑1 (MQ=255)
                                                                                                                                tATTTTGTGGGTGAAGGTAAAGCAGTTGAAATTGCGGAAGCTGTCAAAGCGACGGGTGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGCCCGGCGCAAGAGCGTAACCTGGAGCGTTTGTGCGAGTGTCGTGTTATCGa       <  2:1214947/140‑1 (MQ=255)
                                                                                                                                 aTTTTGTGGGTGAAGGTAAAGCAGTTGAAATTGCGGAAGCTGTCAAAGCGACGGGTGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGCCCGGCGCAAGAGCGTAACCTGGAGCGTTTGTGCGAgtg                   >  2:1173211/1‑127 (MQ=255)
                                                                                                                                 aTTTTGTGGGTGAAGGTAAAGCAGTTGAAATTGCGGAAGCTGTCAAAGCGACGGGTGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGCCCGGCGCAAGAGCGTAACCTGGAGCGTTTGTGCGAgtg                   <  1:1173211/127‑1 (MQ=255)
                                                                                                                                  ttttGTGGGTGAAGGTAAAGCAGTTGAAATTGCGGAAGCTGTCAAAGCGACGGGTGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGCCCGGCGCAAGAGCGTAACCTGGAGCGTTTGTGCGAGTGTCGTGTTATCGAcc     >  2:478576/1‑140 (MQ=255)
                                                                                                                                    ttGTGGGTGAAGGTAAAGCAGTTGAAATTGCGGAAGCTGTCAAAGCGACGGGTGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGCCCGGCGCAAGAGCGTAACCTGGAGCGTTTGTGCGAGTGTCGTGtt            <  1:581632/131‑1 (MQ=255)
                                                                                                                                    ttGTGGGTGAAGGTAAAGCAGTTGAAATTGCGGAAGCTGTCAAAGCGACGGGTGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGCCCGGCGCAAGAGCGTAACCTGGAGCGTTTGTGCGAGTGTCGTGtt            >  2:581632/1‑131 (MQ=255)
                                                                                                                                     tgtgGGTGAAGGTAAAGCAGTTGAAATTGCGGAAGCTGTCAAAGCGACGGGTGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGCCCGGCGCAAGAGCGTAACCTGGAGCGTTTGTGCGAGTGTCGTGTTATCGACCGCa  <  1:766801/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                        
TGGTACACATCTATTTTACGCAAGACAAAGATATGGAAGACCTCCAGGAGTTTGAATCTCTGGTCTCTTCCGCCGGTGTCGAAGCATTGCAGGTGATTACCGGTAGCCGTAAAGCGCCGCACCCAAAGTATTTTGTAGGTGAAGGTAAAGCAGTTGAAATTGCGGAAGCTGTCAAAGCGACGGGTGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGCCCGGCGCAAGAGCGTAACCTGGAGCGTTTGTGCGAGTGTCGTGTTATCGACCGCA  >  NC_000913/4400709‑4400981

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A