Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F28 I1 R1	108	95.2	3177646	99.2%	3152224	139.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,372,012	A→G	T330A (ACC→GCC)	groL →	chaperonin GroEL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,372,012	0	A	G	100.0%	233.5 / NA	62	T330A (ACC→GCC)	groL	chaperonin GroEL
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (41/21); total (41/21)

TCGTAAAGCTATGCTGCAGGATATCGCAACCCTGACTGGCGGTACCGTGATCTCTGAAGAGATCGGTATGGAGCTGGAAAAAGCAACCCTGGAAGACCTGGGTCAGGCTAAACGTGTTGTGATCAACAAAGACACCACCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGTGTTGCTCAGATCCGTCAGCAGATTGAAGAAGCAACTTCTGACTACGACCGTGAAAAACTGCAGGAACGCGTAGCGAAACTGGCAGGC  >  NC_000913/4371876‑4372146

gcgtAAAGCTATGCTGAAGGATATCGCACCCCTGACGGGCGGTACCGTGATCTCTGAAGAGAGCGGTATGGAGCGGGAAAAACCACCCCGGGAAGCCCTGGGTCAGGCTAAACGTGTTGTGATAAACAAAGAcaccgcca                                                                                                                                     <  2:960631/139‑1 (MQ=255)

              tgcAGGATATCGCAACCCTGACTGGCGGTACCGTGATCTCTGAAGAGATCGGTATGGAGCTGGAAAAAGCAACCCTGGAAGACCTGGGTCAGGCTAAACGTGTTGTGATCAACAAAGACACCGCCACTATCATCGATGGc                                                                                                                       >  1:1416067/1‑140 (MQ=255)

              ggcAGGATATCGCACCCCTGCCTGGCGGTACCGTGATCTCTGAAGAGATGGGTATGGAGCGGAAAAAAGCACCCCTGGAAGACCTGGGTCAGGCAAAACGTGTTGTGATCAAAAAAGACACCGCCACTATCATCGATGGc                                                                                                                       <  2:1246064/139‑1 (MQ=255)

                     tatCGCAACCCTGACTGGCGGTACCGTGATCTCTGAAGAGATCGGTATGGAGCTGGAAAAAGCAACCCTGGAAGACCTGGGGCAGGCTAAACGTGTTGTGATCAACAAAGACACCGCCACTATCATCGATGGCGTGGGTg                                                                                                                >  1:69922/1‑140 (MQ=255)

                      ctCGCACCCGTGACTGGGGGTCCCGTGTTCTCTGAAGAGCGCGGTAGGGGGCAGGAAAAAGCAACCCTGGAAGACCTGGGACAGTCTAAGCGTGTTGTGATAAACAAAGACACCGCCACTATCATCGATGGCGTGGGTga                                                                                                               <  2:722548/139‑1 (MQ=255)

                      atCGCAACCCTGACTGGCGGTACCGTGATCTCTGAAGAGATCGGTATGGAGCTGGAAAAAGCAACCCTGGAAGACCTGGGTCAGGCTAAACGGGTTGTGATCAACAAAGACACCGCCACTATCATCGATGGCGTGGGTga                                                                                                               >  1:1532138/1‑140 (MQ=255)

                         gCACCCCTGACTGGCGGTACCGGGATCTCTGAAGAGATGGGTATGGAGCTGAAAAACGCACCCCGGGAAGACCTGGGTCAGGCTAACCGTTTTGTAAAAAAAAAAGCCACCGCCACTATCATCGATGGCGTGGGTgaaga                                                                                                            <  2:1150909/140‑1 (MQ=255)

                          cAACCCTGACTGGCGGTACCGTGATCTCTGAAGAGATCGGTATGGAGCTGGAAAAAGCAACCCTGGAAGACCTGGGTCAGGCTAAACGTGTTGTGATCAACAAAGACACCGCCACTATCATCGATGGCGTGGGTgaagaa                                                                                                           >  1:1216178/1‑140 (MQ=255)

                             cccTGACTGGCGGTACCGTGATCTCTGAAGAGATCGGTATGGAGCTGGAAAAAGCAACCCTGGAAGACCTGGGTCAGGCTAAACGTGTTGTGATCAACAAAGACACCGCCACTATCATCGATGGCGTGGGTGAAGAAGCt                                                                                                        >  1:1235619/1‑140 (MQ=255)

                             cccTGACTGGCGGTACCGTGATCTCTGAAAAGAGCGGTATGGAGCTGGAAAAAGCAACCCCCGAAGACCTGGGGCCGGCTAAACCTGTTGTGATCAACAAAGACACCGCCACTATCATCGATGGCGTGGGGGAAGAAGcc                                                                                                        >  2:1437706/1‑139 (MQ=255)

                              ccggACGGGGGGTGCCGTGATCTTGGAAGGTCTCGGGATGGGGCTGAAAAAAACACCCCGGGAAGACCCGGGTGAGGCTAACGGTGTTGTAACCAAAAAGGACGCCGCCACTATCATCGATGGCGTGGGTGAAGAAGCTg                                                                                                       <  2:1051733/137‑1 (MQ=255)

                              ccTGACTGGCGGTCCCGTGATCTCTGAAGAGATGGGTATGGAGCTGAAAAAAGCACCCCTGGAAGCCCGGGGTCAGGCTAAACGTGTTGTGATCAACAAAGACACCGCCACTATCATCGATGGCGTGGGTGAAGAAGCTg                                                                                                       <  2:1416067/140‑1 (MQ=255)

                              ccTGACGGGCGGTCCCGTGATCTCTGAAGAGATGGGTAGGGAGCGGGAAAAAGAACCCCGGGAAGCCCTGGGTCAGGCAAAACGTGTTGTGATCAACAAAGACACCGCCACTATCATCGATGGCGTGGGTGAAGAAGCTg                                                                                                       <  2:1115079/140‑1 (MQ=255)

                              ccTGACGGGCGGTACCGTGATCTCTGAAGAGATGGGTAGGGAGCGGAAAAAAGCACCCCTGGAAGACCGGGGTCAGGTTAACCGTGTTGTGATCAACAAAGACACCGCCACTATCATCGATGGCGTGGGTGAAGAAGCTg                                                                                                       <  2:788736/140‑1 (MQ=255)

                                  aCTGGCGGTACCGTGATCTCTGAAGAGATCGGTATGGAGCTGGAAAAAGCAACCCTGGAAGACCTGGGTCAGGCTAAACGTGTTGTGATCAACAAAGACACCGCCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAAt                                                                                                   >  1:344901/1‑140 (MQ=255)

                                     ggCGGTACCGTGATCTCTGAAGAGATCGGTATGGAGCTGGAAAAAGCAACCCTGGAAGCCCTGGGTCAGGCTAAACGTGTTGTGATCAACAAAGACACCGCCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCa                                                                                                <  1:1367917/140‑1 (MQ=255)

                                     ggCGGTACCGTGATCTCTGAAGAGATCGGTATGGAGCTGGAAAAAGCAACCCTGGAAGACCTGGGTCAGGCTAAACGTGTTGTGATCAACAAAGACACCGCCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCa                                                                                                >  1:344800/1‑140 (MQ=255)

                                       cGGTACCGTGATCTCTGAAGAGATCGGTATGGAGCTGGAAAAAGCAACCCTGGAAGACCTGGGTCAGGCTAAACGTGTTGTGATCAACAAAGACACCGCCACTATCATCGATGGCGTGGGTGAAGGAGCTGCAATCCAgg                                                                                              >  1:1372832/1‑140 (MQ=255)

                                            ccGTGATCTCTGAAGAGATCGGTATGGAGCTGGAAAAAGCAACCCTGGAAGACCTGGGTCAGGCTAAACGTGTTGTGATCAACAAAGACACCGCCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCgt                                                                                         >  1:1405224/1‑140 (MQ=255)

                                            ccGTGATCTCTGAAGAGATCGGTATGGAGCTGGAAAAAGCAACCCTGGAAGACCTGGGTCAGGCTAAACGTGTTGTGATCAACAAAGACACCGCCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCgt                                                                                         >  2:492405/1‑140 (MQ=255)

                                                gATCTCTGAAGAGATCGGTATGGAGCTGGAAAAAGCACCCCTGGAAGCCCTGGGTCAGGCTAAACGTGTTGTGATCAACAAAGACA‑CGCCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGTGTTGc                                                                                    <  2:980604/140‑1 (MQ=255)

                                                    tgtGAAGAGATGGGTATGGGGCTGAAAAAAGCACCCCGGGAAGCCCGGGGCCGGGCTAAACGTGTTGTGATAAACAAAGACACCGCCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGTGTTGCTCa                                                                                 <  2:122759/138‑1 (MQ=255)

                                                    tctGAAGAGATCGGTATGGAGCTGGAAAAAGCAACCCTGGAAGACCTGGGTCAGGCTAAACGTGTTGTGATCAACAAAGACACCGCCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGTGTTGCTCa                                                                                 >  2:56458/1‑140 (MQ=255)

                                                           agaTCGGTATGGAGCTGGAAAAAGCAACCCTGGAAGACCTGGGTCAGGCTAAACGTGTTGTGATCAACAAAGACACCGCCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGTGTTGCTCAGATCCGt                                                                          >  2:689766/1‑140 (MQ=255)

                                                           agaTCGGTATGGAGCGGAAAAAAGCACCCCTGGAAGCCCTGGGTCAGGCTAAACGTGTTGTGATCAACAAAGACACCGCCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGTGTTGCTCAGATCCGt                                                                          <  1:849749/140‑1 (MQ=255)

                                                             aTCGGTATGGAGCTGGAAAAAGCAACCCTGGAAGACCTGGGTCAGGCTAAACGTGTTGTGATCAACAAAGACACCGCCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGTGTTGCTCAGATCCGTca                                                                        >  1:594326/1‑140 (MQ=255)

                                                             aTCGGTATGGAGCTGGAAAAAGCAACCCTGGAAGACCTGGGTCAGGCTAAACGTGTTGTGATCAACAAAGACACCGCCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGTGTTGCTCAGATCCGTca                                                                        >  1:790394/1‑140 (MQ=255)

                                                             aTCGGTATGGAGCTGGAAAAAGCAACCCTGGAAGACCTGGGTCAGGCTAAACGTGTTGTGATCAACAAAGACACCGCCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGTGTTGCTCAGATCCGTca                                                                        >  2:1071795/1‑140 (MQ=255)

                                                             aTCGGTATGGAGCTGAAAAAAGCACCCCGGGAAGCCCGGGGTCAGGCAAAACGTGTTGTGATCAACAAAGACACCGCCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGTGTTGCTCAGATCCGTca                                                                        <  2:673194/140‑1 (MQ=255)

                                                              tCGGTATGGAGCTGGAAAAAGCACCCCTGGAAGACCGGGGTCAGGCTAAACGTGTTGTGATCAACAAAGACACCGCCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGTGTTGCTCAGATCCGTcag                                                                       <  1:732670/140‑1 (MQ=255)

                                                              tCGGTATGGAGCGGAAAAAAGCACCCCTGGAAGCCCTGGGTCAGGCTAAACGTGTTGTGATCAACAAAGACACCGCCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGTGTTGCTCAGATCCGTcag                                                                       <  2:1079737/140‑1 (MQ=255)

                                                                       aGCTGGAAAAAGCAACCCTGGAAGACCTGGGTCAGGCTAAACGTGTTGTGATCAACAAAGACACCGCCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGTGTTGCTCAGATCCGTCAGCAGATTgaa                                                              >  1:1497629/1‑140 (MQ=255)

                                                                            gAAAAAGCAACCCTGGAAGACCTGGGTCAGGCTAAACGTGTTGTGATCAACAAAGACACCGCCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGTGTTGCTCAGATCCGTCAGCAGATTGAAGAAGc                                                         >  2:847319/1‑140 (MQ=255)

                                                                            gAAAAAGCAACCCTGGAAGACCTGGGTCAGGCTAAACGTGTTGTGATCAACAAAGACACCGCCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGTGTTGCTCAGATCCGTCAGCAGATTGAAGAAGc                                                         >  1:136850/1‑140 (MQ=255)

                                                                            gAAAAAGCAACCCTGGAAGACCTGGGTCAGGCTAAACGTGTTGTGATCAACAAAGACACCGCCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGTGTTGCTCAGATCCGTCAGCAGATTGAAGAAGc                                                         >  1:157786/1‑140 (MQ=255)

                                                                            gAAAAAGCAACCCTGGAAGACCTGGGTCAGGCTAAACGTGTTGTGATCAACAAAGACACCGCCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGTGTTGCTCAGATCCGTCAGCAGATTGAAGAAGc                                                         >  2:1456736/1‑140 (MQ=255)

                                                                                 aGCACCCCTGGAAGCCCTGGGTCAGGCTAAACGTGTTGTGATCAACAAAGACACCGCCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGTGTTGCTCAGATCCGTCAGCAGATTGAAGAAGCAACtt                                                    <  1:656239/140‑1 (MQ=255)

                                                                                           gAAGACCGGGGTCGGGCTAAGCGTGTTGTGATCAACAAAGCCACCGCCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGTGTTGCTCAGATCCGTCAGCAGATTGAAGAAGCAACTTCTGACTACGa                                          <  1:192562/140‑1 (MQ=255)

                                                                                                ccTGGGTCAGGCTAAACGTGTTGTGATCAACAAAGACACCGCCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGTGTTGCTCAGATCCGTCAGCAGATTGAAGAAGCAACTTCTGACTACGACCGTg                                     >  1:1239856/1‑140 (MQ=255)

                                                                                                ccTGGGTCAGGCTAAACGTGTTGTGATCAACAAAGACACCGCCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGTGTTGCTCAGATCCGTCAGCAGATTGAAGAAGCAACTTCTGACTACGACCGTg                                     >  1:1534949/1‑140 (MQ=255)

                                                                                                ccTGGGTCAGGCTAAACGTGTTGTGATCAACAAAGACACCGCCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGTGTTGCTCAGATCCGTCAGCAGATTGAAGAAGCAACTTCTGACTACGACCGTg                                     >  2:1367969/1‑140 (MQ=255)

                                                                                                 cTGGGTCAGGCTAAACGTGTTGTGATCAACAAAGACACCGCCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGTGTTGCTCAGATCCGTCAGCAGATTGAAGAAGCAACTTCTGACTACGACCGTGa                                    >  1:1021481/1‑140 (MQ=255)

                                                                                                 cTGGGTCAGGCTAAACGTGTTGTGATCAACAAAGACACCGCCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGTGTTGCTCAGATCCGTCAGCAGATTGAAGAAGCAACTTCTGACTACGACCGTGa                                    >  2:328482/1‑140 (MQ=255)

                                                                                                 cTGGGTCAGGCTAAACGTGTTGTGATCAACAAAGACACCGCCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGTGTTGCTCAGATCCGTCAGCAGATTGAAGAAGCAACTTCTGACTACGACCGTGa                                    >  1:663067/1‑140 (MQ=255)

                                                                                                         ggCTAAACGTGTTGTGATCAACAAAGACACCGCCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGTGTTGCTCAGATCCGTCAGCAGATTGAAGAAGCAACTTCTGACTACGACCGTGAAAAACTGc                            >  2:1344575/1‑140 (MQ=255)

                                                                                                         ggCTAAACGTGTTGTGATCAACAAAGACACCGCCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGTGTTGCTCAGATCCGTCAGCAGATTGAAGAAGCAACTTCTGACTACGACCGTGAAAAACTGc                            >  2:528569/1‑140 (MQ=255)

                                                                                                          gCTAAACGTGTTGTGATCAACAAAGACACCGCCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGTGTTGCTCAGATCCGTCAGCAGATTGAAGAAGCAACTTCTGACTACGACCGTGAAAAACTGCa                           >  2:705066/1‑140 (MQ=255)

                                                                                                          gCTAAACGTGTTGTGATCAACAAAGACACCGCCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGTGTTGCTCAGATCCGTCAGCAGATTGAAGAAGCAACTTCTGACTACGACCGTGAAAAACTGCa                           >  1:441236/1‑140 (MQ=255)

                                                                                                          gCTAAACGTGTTGTGATCAACAAAGACACCGCCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGTGTTGCTCAGATCCGTCAGCAGACTGAAGAAGCAACTTCTGACTACGACCGTGAAAAACTGCa                           >  1:1059673/1‑140 (MQ=255)

                                                                                                            tAAACGTGTTGTGATCAACAAAGACACCGCCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGTGTTGCTCAGATCCGTCAGCAGATTGAAGAAGCAACTTCTGACTACGACCGTGAAAAACTGCAgg                         <  2:136850/140‑1 (MQ=255)

                                                                                                             aaaGGTGTTGTGACAAACAAAAACACCGCCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGTGTTGCTCAGATCCGTCAGCAGATTGAAGAAGCAACTTCTGACTACGACCGTGAAAAACTGCAGGa                        <  2:929455/140‑1 (MQ=255)

                                                                                                              aaCGTGTTGTGATCAACAAAGACACCGCCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGTGTTGCTCAGATCCGTCAGCAGATTGAAGAAGCAACTTCTGACTACGACCGTGAAAAACTGCAGGaa                       >  1:1073026/1‑140 (MQ=255)

                                                                                                                    ttgtGTTCAACAAAGACACCGCCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGTGTTGCTCAGATCCGTCAGCAGATTGAAGAAGCAACTTCTGACTACGACCGTGAAAAACTGCAGGAACGCGTa                 >  2:175353/1‑140 (MQ=255)

                                                                                                                    ttgtGATCAACAAAGACACCGCCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGTGTTGCTCAGATCCGTCAGCAGATTGAAGAAGCAACTTCTGACTACGACCGTGAAAAACTGCAGGATCGCGTa                 >  2:1149447/1‑140 (MQ=255)

                                                                                                                    ttgtGATCAACAAAGACACCGCCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGTGTTGCTCAGATCCGTCAGCAGATTGAAGAAGCAACTTCTGACTACGACCGTGAAAAACTGCAGGAACGCGTa                 >  1:625887/1‑140 (MQ=255)

                                                                                                                    ttgtGATCAACAAAGACACCGCCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGTGTTGCTCAGATCCGTCAGCAGATTGAAGAAGCAACTTCTGACTACGACCGTGAAAAACTGCAGGAACGCGTa                 >  1:954343/1‑140 (MQ=255)

                                                                                                                    ttgtGATCAACAAAGACACCGCCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGTGTTGCTCAGATCCGTCAGCAGATTGAAGAAGCAACTTCTGACTACGACCGTGAAAAACTGCAGGAACGCGTa                 >  2:1062809/1‑140 (MQ=255)

                                                                                                                    ttgtGATCAACAAAGACACCGCCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGTGTTGCTCAGATCCGTCAGCAGATTGAAGAAGCAACTTCTGACTACGACCGTGAAAAACTGCAGGAACGCGTa                 >  2:917458/1‑140 (MQ=255)

                                                                                                                    ttgtGATCAACAAAGACACCGCCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGTGTTGCTCAGATCCGCCAGCAGATTGAAGAAGCAACTTCTGACTACGACCGTGAAAAACTGCAGGAACGCGTa                 >  2:22561/1‑140 (MQ=255)

                                                                                                                          tCAACAAAGACACCGCCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGTGTTGCTCAGATCCGTCAGCAGATTGAAGAAGCAACTTCTGACTACGACCGTGAAAAACTGCAGGAACGCGTAGCGaaa           <  1:423726/140‑1 (MQ=255)

                                                                                                                           caacaaAGCCACCGCCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGTGTTGCTCAGATCCGTCAGCAGATTGAAGAAGCAACTTCTGACTACGACCGTGAAAAACTGCAGGAACGCGTAGCGAAAc          <  2:69922/140‑1 (MQ=255)

                                                                                                                            aacaaAGACACCGCCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGTGTTGCTCAGATCCGTCAGCAGATTGAAGAAGCAACTTCTGACTACGACCGTGAAAAACTGCAGGAACGCGTAGCGAAACt         >  2:190740/1‑140 (MQ=255)

                                                                                                                              caaAGACACCGCCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGTGTTGCTCAGATCCGTCAGCAGATTGAAGAAGCAACTTCTGACTACGACCGTGAAAAACTGCAGGAACGCGTAGCGAAACTgg       >  2:1451238/1‑140 (MQ=255)

                                                                                                                                aaGACACCGCCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGTGTTGCTCAGATCCGTCAGCAGATTGAAGAAGCAACTTCTGACTACGACCGTGAAAAACTGCAGGAACGCGTAGCGAAACTGGCa     <  2:663067/140‑1 (MQ=255)

                                                                                                                                   acacCGCCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGTGTTGCTCAGATCCGTCAGCAGATTGAAGAAGCAACTTCTGACTACGACCGTGAAAAACTGCAGGAACGCGTAGCGAAACTGGCAggc  >  2:918293/1‑140 (MQ=255)

TCGTAAAGCTATGCTGCAGGATATCGCAACCCTGACTGGCGGTACCGTGATCTCTGAAGAGATCGGTATGGAGCTGGAAAAAGCAACCCTGGAAGACCTGGGTCAGGCTAAACGTGTTGTGATCAACAAAGACACCACCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGTGTTGCTCAGATCCGTCAGCAGATTGAAGAAGCAACTTCTGACTACGACCGTGAAAAACTGCAGGAACGCGTAGCGAAACTGGCAGGC  >  NC_000913/4371876‑4372146

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A