Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	123	51.0	1731148	99.3%	1719029	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,575,536	A→G	pseudogene (1402/2721 nt)	yjiV →	putative uncharacterized protein YjiV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,575,536	0	A	G	100.0%	173.1 / NA	48	pseudogene (1402/2721 nt)	yjiV	putative uncharacterized protein YjiV
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (28/20); total (28/20)

TCCTGAAGATAATCAGAACAGACGCTGGCCTCAAATTCGTAATGGCAACGTATCCCATCGTTTAGCGAAGTTGCTGATGCTGGGGGCTGGATTCAAAACCGTCAATGCGGCAACTATTGATATTATCAATACATGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAAGAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCA  >  NC_000913/4575406‑4575671

tCCTGAAGATAATCAGAACAGACGCTGGCCTCAAATTCGTAATGGCAACGTATCCCATCGTTTAGCGAAGTTGCTGATGCTGGGGGCTGGATTCAAAACCGTCAATGCGGCAACTATTGATATTATCAATGCATGGCTGa                                                                                                                                >  2:382840/1‑140 (MQ=255)

         tAATCAGAACAGACGCTGGCCTCAAATTCGTAATGGCAACGTATCCCATCGTTTAGCGAAGTTGCTGATGCTGGGGGCTGGATTCAAAACCGTCAATGCGGCAACTATTGATATTATCAATGCATGGCTGAAAGAAGCAt                                                                                                                       <  1:382840/140‑1 (MQ=255)

         tAATCAGAACAGACGCTGGCCTCAAATTCGTAATGGCAACGTATCCCATCGTTTAGCGAAGTTGCTGATGCTGGGGGCTGGATTCAAAACCGTCAATGCGGCAACTATTGATATTATCAATGCATGGCTGAAAGAAGCAt                                                                                                                       <  1:659384/140‑1 (MQ=255)

          aaTCAGAACAGACGCTGGCCTTAAATTCGTAATGGCAACGTATCCCATCGTTTAGCGAAGTTGCTGATGCTGGGGGCTGGATTCAAAACCGTCAATGCGGCAACTATTGATATTATCAATGCATGGCTGGAAGAAGCATg                                                                                                                      >  1:806653/1‑140 (MQ=255)

          aaTCAGAACAGACGCTGGCCTCAAATTCGTAATGGCAACGTATCCCATCGTTTAGCGAAGTTGCTGATGCTGGGGGCTGGATTCAAAACCGTCAATGCGGCAACTATTGATATTATCAATGCATGGCTGAAAGAAGCATg                                                                                                                      >  1:264059/1‑140 (MQ=255)

            tCAGAACAGACGCTGGCCTCAAATTCGTAATGGCAACGTATCCCATCGTTTAGCGAAGTTGCTGATGCTGGGGGCTGGATTCAAAACCGTCAATGCGGCAACTATTGATATTATCAATGCATGGCTGAAAGAAGCATggg                                                                                                                    <  2:264059/140‑1 (MQ=255)

                 aCAGACGCTGGCCTCAAATTCGTAATGGCAACGTATCCCATCGTTTAGCGAAGTTGCTGATGCTGGGGGCTGGATTCAAAACCGTCAATGCGGCAACTATTGATATTATCAATGCATGGCTGAAAGAAGCATGGGCCCaa                                                                                                               >  2:197552/1‑140 (MQ=255)

                     aCGCTGGCCTCAAATTCGTAATGGCAACGTATCCCATCGTTTAGCGAAGTTGCTGATGCTGGGGGCTGGATTCAAAACCGTCAATGCGGCAACTATTGATATTATCAATGCATGGCTGAAAGAAGCATGGGcc                                                                                                                  <  2:713638/133‑1 (MQ=255)

                     aCGCTGGCCTCAAATTCGTAATGGCAACGTATCCCATCGTTTAGCGAAGTTGCTGATGCTGGGGGCTGGATTCAAAACCGTCAATGCGGCAACTATTGATATTATCAATGCATGGCTGAAAGAAGCATGGGcc                                                                                                                  >  1:713638/1‑133 (MQ=255)

                     aCGCTGGCCTCAAATTCGTAATGGCAACGTATCCCATCGTTTAGCGAAGTTGCTGATGCTGGGGGCTGGATTCAAAACCGTCAATGCGGCAACTATTGATATTATCAATGCATGGCTGAAAGAAGCATGGGCCCAACTTa                                                                                                           >  2:169360/1‑140 (MQ=255)

                     aCGCTGGCCTCAAATTCGTAATGGCAACGTATCCCATCGTTTAGCGAAGTTGCTGATGCTGGGGGCTGGATTCAAAACCGTCAATGCGGCAACTATTGATATTATCAATGCATGGCTGAAAGAAGCATGGGCCCAACTTa                                                                                                           >  2:580073/1‑140 (MQ=255)

                     aCGCTGGCCTCAAATTCGTAATGGCAACGTATCCCATCGTTTAGCGAAGTTGCTGATGCTGGGGGCTGGATTCAAAACCGTCAATGCGGCAACTATTGATATTATCAATGCATGGCTGAAAGAAGCATGGGCCCAACTTa                                                                                                           >  2:606108/1‑140 (MQ=255)

                     aCGCTGGCCTCAAATTCGTAATGGCAACGTATCCCATCGTTTAGCGAAGTTGCTGATGCTGGGGGCTGGATTCAAAACCGTCAATGCGGCAACTATTGATATTATCAATGCATGGCTGAAAGAAGCATGGGCCCAACTTa                                                                                                           >  2:609771/1‑140 (MQ=255)

                          ggCCTCAAATTCGTAATGGCAACGTATCCCATCGTTTAGCGAAGTTGCTGATGCTGGGGGCTGGATTCAAAACCGTCAATGCGGCAACTATTGATATTATCAATGCATGGCTGAAAGAAGCATGGGCCCAACTTACCGGa                                                                                                      >  1:560791/1‑140 (MQ=255)

                            ccTCAAATTCGTAATGGCAACGTATCCCATCGTTTAGCGAAGTTGCTGATGCTGGGGGCTGGATTCAAAACCGTCAATGCGGCAACTATTGATATTATCAATGCATGGCTGAAAGAAGCATGGGCCCAACTTACCGGAcc                                                                                                    <  1:580073/140‑1 (MQ=255)

                              tCAAATTCGTAATGGCAACGTATCCCATCGTTTAGCGAAGTTGCTGATGCTGGGGGCTGGATTCAAAACCGTCAATGCGGCAACTATTGATATTATCAATGCATGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGc                                                                                                  <  1:527470/140‑1 (MQ=255)

                               cAAATTCGTAATGGCAACGTATCCCATCGTTTAGCGAAGTTGCTGATGCTGGGGGCTGGATTCAAAACCGTCAATGCGGCAACTATTGATATTATCAATGCATGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCt                                                                                                 <  2:662672/140‑1 (MQ=255)

                                                      ccATCGTTTAGCGAAGTTGCTGATGCTGGGGGCTGGATTCAAAACCGTCAATGCGGCAACTATTGATATTATCAATGCATGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCa                                                                          >  2:44678/1‑140 (MQ=255)

                                                      ccATCGTTTAGCGAAGTTGCTGATGCTGGGGGCTGGATTCAAAACCGTCAATGCGGCAACTATTGATATTATCAATGCATGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCa                                                                          >  2:426799/1‑140 (MQ=255)

                                                          cGTTTAGCGAAGTTGCTGATGCTGGGGGCTGGATTCAAAACCGTCAATGCGGCAACTATTGATATTATCAATGCATGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCg                                                                      >  1:23284/1‑140 (MQ=255)

                                                           gTTTAGCGAAGTTGCTGATGCTGGGGGCTGGATTCAAAACCGTCAATGCGGCAACTATTGATATTATCAATGCATGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAAc                                                                        >  1:556892/1‑137 (MQ=255)

                                                           gTTTAGCGAAGTTGCTGATGCTGGGGGCTGGATTCAAAACCGTCAATGCGGCAACTATTGATATTATCAATGCATGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAAc                                                                        <  2:556892/137‑1 (MQ=255)

                                                            tttAGCGAAGTTGCTGATGCTGGGGGCTGGATTCAAAACCGTCAATGCGGCAACTATTGATATTATCAATGCATGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGtt                                                                    >  2:310852/1‑140 (MQ=255)

                                                              tAGCGAAGTTGCTGATGCTGGGGGCTGGATTCAAAACCGTCAATGCGGCAACTATTGATATTATCAATGCATGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGtttt                                                                  >  1:731979/1‑140 (MQ=255)

                                                                gCGAAGTTGCTGATGCTGGGGGCTGGATTCAAAACCGTCAATGCGGCAACTATTGATATTATCAATGCATGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTttta                                                                <  1:477545/140‑1 (MQ=255)

                                                                     gTTGCTGATGCTGGGGGCTGGATTCAAAACCGTCAATGCGGCAACTATTGATATTATCAATGCATGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTAc                                                           <  1:126092/140‑1 (MQ=255)

                                                                      ttGCTGATGCTGGGGGCTGGATTCAAAACCGTCAATGCGGCAACTATTGATATTATCAATGCATGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTAcc                                                          >  1:100827/1‑140 (MQ=255)

                                                                              gCTGGGGGCTGGATTCAAAACCGTCAATGCGGCAACTATTGATATTATCAATGCATGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTtttattt                                                             >  2:736456/1‑129 (MQ=255)

                                                                              gCTGGGGGCTGGATTCAAAACCGTCAATGCGGCAACTATTGATATTATCAATGCATGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTtttattt                                                             <  1:736456/129‑1 (MQ=255)

                                                                                tGGGGGCTGGATTCAAAACCGTCAATGCGGCAACTATTGATATTATCAATGCATGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAAGAGCat                                                <  1:426799/140‑1 (MQ=255)

                                                                                                   cGTCAATGCGGCAACTATTGATATTATCAATGCATGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGGTTTTATTTACCGAAAGAGCATATGACTTTTTCTTTAATCa                             <  2:275819/140‑1 (MQ=255)

                                                                                                     tCAATGCGGCAACTATTGATATTATCAATGCATGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAAGAGCATATGACTTTTTCTTTAATCACg                           >  1:791602/1‑140 (MQ=255)

                                                                                                     tCAATGCGGCAACTATTGATATTATCAATGCATGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAAGAGCATATGACTTTTTCTTTAATCACg                           <  2:731979/140‑1 (MQ=255)

                                                                                                     tCAATGCGGCAACTATTGATATTATCAATGCATGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAAGAGCATATGACTTTTTCTTTAATCACg                           <  1:606108/140‑1 (MQ=255)

                                                                                                     tCAATGCGGCAACTATTGATATTATCAATGCATGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAAGAGCATATGACTTTTTCTTTAATCACg                           <  1:609771/140‑1 (MQ=255)

                                                                                                     tCAATGCGGCAACTATTGATATTATCAATGCATGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAAGAGCATATGACTTTTTCTTTAATCACg                           <  2:23284/140‑1 (MQ=255)

                                                                                                                 cTATTGATATTATCAATGCATGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAAGAGCATATGACTTTTTCTTTAATCACGGATGCCTGGAtt               <  1:15915/140‑1 (MQ=255)

                                                                                                                  tATTGATATTATCAATGCATGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAAGAGCATATGACTTTTTCTTTAATCACGGATGCCTGGAttt              <  2:806653/140‑1 (MQ=255)

                                                                                                                            atCAATGCATGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAAGAGCATATGACTTTTTCTTTAATCACGGATGc                      >  1:96491/1‑122 (MQ=255)

                                                                                                                            atCAATGCATGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAAGAGCATATGACTTTTTCTTTAATCACGGATGc                      <  2:96491/122‑1 (MQ=255)

                                                                                                                            atCAATGCATGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAAGAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAAc    >  1:657477/1‑140 (MQ=255)

                                                                                                                            atCAATGCATGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAAGAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAAc    >  1:548159/1‑140 (MQ=255)

                                                                                                                            atCAATGCATGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAAGAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAAc    >  1:671021/1‑140 (MQ=255)

                                                                                                                            atCAATGCATGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAAGAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAAc    >  1:230962/1‑140 (MQ=255)

                                                                                                                            atCAATGCATGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAAGAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAAc    >  2:740369/1‑140 (MQ=255)

                                                                                                                            atCAATGCATGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAAGAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAAc    >  2:853561/1‑140 (MQ=255)

                                                                                                                              cAATGCATGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAAGAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCa  >  2:655393/1‑140 (MQ=255)

                                                                                                                              cAATGCATGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAAGAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCa  >  1:675965/1‑140 (MQ=255)

TCCTGAAGATAATCAGAACAGACGCTGGCCTCAAATTCGTAATGGCAACGTATCCCATCGTTTAGCGAAGTTGCTGATGCTGGGGGCTGGATTCAAAACCGTCAATGCGGCAACTATTGATATTATCAATACATGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAAGAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCA  >  NC_000913/4575406‑4575671

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A