Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A12 F28 I1 R1
|
123 |
51.0 |
1731148 |
99.3% |
1719029 |
138.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,606,094 |
(G)8→9 |
noncoding (72/87 nt) |
leuV ← |
tRNA‑Leu |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,606,086 | 1 | . | G | 94.7%
| 127.6
/ 0.4
| 38 | noncoding (80/87 nt) | leuV | tRNA‑Leu |
| Reads supporting (aligned to +/- strand): ref base . (0/2); new base G (14/22); total (14/24) |
| Fisher's exact test for biased strand distribution p-value = 5.22e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.73e-01 |
CACAAACAGTTGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAG‑AAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA > NC_000913/4605948‑4606207
|
cacaAACAGTTGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAG‑AAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑g > 2:230617/1‑140 (MQ=255)
cacaAACAGTTGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAG‑AAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑g < 1:572949/140‑1 (MQ=255)
caAACAGTTGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAG‑AAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑ggg > 2:200170/1‑140 (MQ=255)
aaCAGTTGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAG‑AAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑ggggg > 2:440574/1‑140 (MQ=255)
aCAGTTGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAG‑AAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑gggggg < 2:540526/140‑1 (MQ=255)
aGTTGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAG‑AAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑gggggggg > 1:468725/1‑140 (MQ=255)
gTTGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAG‑AAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑ggggggggg < 2:577754/140‑2 (MQ=255)
ttGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAG‑AAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑ggggggggga > 1:784626/1‑138 (MQ=255)
ggCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAG‑AAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGACTTGa < 2:174019/140‑1 (MQ=255)
aCATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAG‑AAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGGACTTGAAcccc < 2:468725/140‑1 (MQ=255)
tttGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAG‑AAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGGACTTGAACCCCCACg < 2:315647/140‑1 (MQ=255)
tGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAG‑AAACAAG‑AAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTcc > 2:357316/1‑140 (MQ=255)
cATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAG‑AAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAg < 1:440574/140‑1 (MQ=255)
cTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAGATCAAGGAAG‑AAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTaa < 2:717923/140‑1 (MQ=255)
tGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAG‑AAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGGACTTGAACCCCCAc > 1:396622/1‑122 (MQ=255)
tGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAG‑AAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGGACTTGAACCCCCAc < 2:396622/122‑1 (MQ=255)
cACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAG‑AAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACAcc > 2:333271/1‑140 (MQ=255)
cACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAG‑AAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGAAACTAACAcc > 2:526758/1‑140 (MQ=255)
aTTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAG‑AAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAg > 2:678960/1‑140 (MQ=255)
cTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAG‑AAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAg < 2:508982/140‑1 (MQ=255)
cTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAG‑AAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAg > 1:609348/1‑140 (MQ=255)
catcatGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAG‑AAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGcgcg > 1:141991/1‑140 (MQ=255)
catcatGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAG‑AAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGcgcg < 1:200170/140‑1 (MQ=255)
atAATGAATTTGAATCAGGGATGAAAAATCAAGGAAG‑AAACAAG‑AAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc < 1:357316/140‑1 (MQ=255)
tAATGAATTTGAATCAGGGATGAAAAATCAAGGAAG‑AAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc < 1:678960/140‑1 (MQ=255)
tAATGAATTTGAATCAGGGATGAAAAATCAAGGAAG‑AAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc < 2:384015/140‑1 (MQ=255)
aaTGAATTTGAATCAGGGATGAAAAATCAAGGAAG‑AAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGcc > 1:524529/1‑140 (MQ=255)
aaTGAATTTGAATCAGGGATGAAAAATCAAGGAAG‑AAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGcc > 1:493919/1‑140 (MQ=255)
aaTGAATTTGAATCAGGGATGAAAAATCAAGGAAG‑AAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc > 1:429179/1‑139 (MQ=255)
aaTGAATTTGAATCAGGGATGAAAAATCAAGGAAG‑AAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc < 2:429179/139‑1 (MQ=255)
aTGAATTTGAATCAGGGATGAAAAATCAAGGAAG‑AAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCa > 1:75105/1‑140 (MQ=255)
tGAATTTGAATCAGGGATGAAAAATCAAGGAAG‑AAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAc < 2:542728/140‑1 (MQ=255)
gAATTTGAATCAGGGATGAAAAATCAAGGAAG‑AAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc > 1:258130/1‑136 (MQ=255)
gAATTTGAATCAGGGATGAAAAATCAAGGAAG‑AAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc < 2:258130/136‑1 (MQ=255)
gAATCAGGGATGAAAAATCAAGGAAG‑AAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcac < 2:6662/140‑1 (MQ=255)
attaaggATGGAAAATCCAGGGAGAAAACAGGAAAGAAATTAACGATACTTGGGCCGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcaca < 2:280779/135‑1 (MQ=14)
aTCAGGGATGAAAAATCAAGGAAG‑AAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcaca < 1:118730/140‑1 (MQ=255)
aTCAGGGATGAAAAATCAAGGAAG‑AAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTGCGcaca < 2:342821/140‑1 (MQ=255)
gggATGAAAAATCAAGGAAG‑AAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCa < 1:367280/140‑1 (MQ=255)
gAAAAATCAAGGAAG‑AAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTa < 1:792950/140‑1 (MQ=255)
tCAAGGAAG‑AAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACtttct > 1:13867/1‑138 (MQ=255)
aaGGAAG‑AAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACttttttt > 1:591864/1‑140 (MQ=255)
aag‑aaACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGata < 2:75105/140‑1 (MQ=255)
aaCAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGc < 2:609348/140‑1 (MQ=255)
aaCAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGc < 2:524529/140‑1 (MQ=255)
aaCAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGc < 1:231234/140‑1 (MQ=255)
tAAAGATAATTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGa < 2:229163/140‑1 (MQ=255)
caaaGATAATTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGa < 1:21025/139‑1 (MQ=255)
|
CACAAACAGTTGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAG‑AAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA > NC_000913/4605948‑4606207
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
