Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	123	51.0	1731148	99.3%	1719029	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,625,160	G→A	R89H (CGC→CAC)	serB →	phosphoserine phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,625,160	0	G	A	100.0%	143.0 / NA	43	R89H (CGC→CAC)	serB	phosphoserine phosphatase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (22/21); total (22/21)

GGTCGTGGGCTGGATAAACAACGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACACGCCTGGCCCACGAAGCGCAGCTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGATATGGACTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAA  >  NC_000913/4625024‑4625289

ggTCGTGGGCTGGATAAACAACGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACACACCt                                                                                                                                >  1:595464/1‑140 (MQ=255)

    gTGGGCTGGATAAACAACGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACACACCTGGcc                                                                                                                            >  1:112716/1‑140 (MQ=255)

     tGGGCTGGATAAACAACGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACACACCTGGccc                                                                                                                           >  2:798455/1‑140 (MQ=255)

            gATAAACAACGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACACACCTgg                                                                                                                              >  2:399498/1‑130 (MQ=255)

            gATAAACAACGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACACACCTgg                                                                                                                              <  1:399498/130‑1 (MQ=255)

            gATAAACAACGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACACACCTGGCCCACGAAgc                                                                                                                    >  1:116840/1‑140 (MQ=255)

            gATAAACAACGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACACACCTGGCCCACGAAgc                                                                                                                    >  1:398278/1‑140 (MQ=255)

             aTAAACAACGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACACACCTGGCCCACGAAgcg                                                                                                                   <  2:595464/140‑1 (MQ=255)

             aTAAACAACGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACACACCTCGCCCACGAAgcg                                                                                                                   <  2:137855/140‑1 (MQ=255)

                       tCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACACACCTGGCCCACGAAGCGCAGCTGGATg                                                                                                         >  2:159609/1‑140 (MQ=255)

                           aCCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACACACCTGGCCCACGAAGCGCAGCTGGATGTCGc                                                                                                     >  2:203053/1‑140 (MQ=255)

                           aCCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACACACCTGGCCCACGAAGCGCAGCTGGATGTCGc                                                                                                     <  1:798455/140‑1 (MQ=255)

                               aaTACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACACACCTGGCCCACGAAGCGCAGCTGGATGTCGCCCCg                                                                                                 <  1:159609/140‑1 (MQ=255)

                               aaTACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACACACCTGGCCCACGAAGCGCAGCTGGATGTCGCCCCg                                                                                                 >  1:198521/1‑140 (MQ=255)

                                 tACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACACACCTGGCCCACGAAGCGCAGCTGGATGTCGCCCCGCt                                                                                               <  2:458088/140‑1 (MQ=255)

                                      gagCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACACACCTGGCCCACGAAGCGCAGCTGGATGTCGCCCCGCTGGGGa                                                                                          >  2:491902/1‑140 (MQ=255)

                                      gagCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGGGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACACACCTGGCCCACGAAGCGCAGCTGGATGTCGCCCCGCTGGGGa                                                                                          >  2:750282/1‑140 (MQ=255)

                                       agCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACACACCTGGCCCACGAAGCGCAGCTGGATGTCGCCCCGCTGGGGaa                                                                                         <  2:116840/140‑1 (MQ=255)

                                           aaCTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACACACCTGGCCCACGAAGCGCAGCTGGATGTCGCCCCGCTGGGGAAAATc                                                                                     >  1:504357/1‑140 (MQ=255)

                                           aaCTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACACACCTGGCCCACGAAGCGCAGCTGGATGTCGCCCCGCTGGGGAAAATc                                                                                     >  1:687168/1‑140 (MQ=255)

                                                         aTGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACACACCTGGCCCACGAAGCGCAGCTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCAc                                                                       <  1:164086/140‑1 (MQ=255)

                                                               aTTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACACACCTGGCCCACGAAGCGCAGCTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCggg                                                                 <  1:491902/140‑1 (MQ=255)

                                                                     gccgccTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACACACCTGGCCCACGAAGCGCAGCTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCt                                                           >  1:545349/1‑140 (MQ=255)

                                                                      ccgccTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACACACCTGGCCCACGAAGCGCAGCTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTg                                                          >  2:106529/1‑140 (MQ=255)

                                                                      ccgccTGGTGCGTGGAAGATTATCAGGTGACTCGTCTGGCAGGTTCACTCACCGCCCGGGCTACACACCTGGCCCACGAAGCGCAGCTGGATGTCGCCCCGCTGGGGAAAATCCCGCAACTGCGCACGCCGGGTTTGCTg                                                          <  2:603276/140‑1 (MQ=255)

                                                                        gccTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACACACCTGGCCCACGAAGCGCAGCTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGt                                                        >  1:745073/1‑140 (MQ=255)

                                                                         ccTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACACACCTGGCCCACGAAGCGCAGCTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTg                                                       >  1:579839/1‑140 (MQ=255)

                                                                          cTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACACACCTGGCCCACGAAGCGCAGCTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGa                                                      <  2:687168/140‑1 (MQ=255)

                                                                          cTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACACACCTGGCCCACGAAGCGCAGCTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGa                                                      <  2:745073/140‑1 (MQ=255)

                                                                                cGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACACACCTGGCCCACGAAGCGCAGCTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGata                                                >  1:850422/1‑140 (MQ=255)

                                                                                  tGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACACACCTGGCCCACGAAGCGCAGCTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGATATg                                              <  2:545349/140‑1 (MQ=255)

                                                                                  tGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACACACCTGGCCCACGAAGCGCAGCTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGATATg                                              >  1:262192/1‑140 (MQ=255)

                                                                                       gATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACACACCTGGCCCACGAAGCGCAGCTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGATATGGACTc                                         <  2:112716/140‑1 (MQ=255)

                                                                                       gATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACACACCTGGCCCACGAAGCGCAGCTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGATATGGACTc                                         <  1:165826/140‑1 (MQ=255)

                                                                                       gATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACACACCTGGCCCACGAAGCGCAGCTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGATATGGACTc                                         <  1:32124/140‑1 (MQ=255)

                                                                                            tCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACACACCTGGCCCACGAAGCGCAGCTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATg                                                    >  2:44464/1‑124 (MQ=255)

                                                                                            tCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACACACCTGGCCCACGAAGCGCAGCTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATg                                                    <  1:44464/124‑1 (MQ=255)

                                                                                               ggTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACACACCTGGCCCACGAAGCGCAGCTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGATATGGACTCCACCGCCa                                 >  2:21444/1‑140 (MQ=255)

                                                                                                   aTTCGTCTGGCAGGTTCACTCACCGCACGGGCTACACACCTGGCCCACGAAGCGCAGCTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGATATGGACTCCACCGCCATCCa                             >  2:795143/1‑140 (MQ=255)

                                                                                                     tCGTCTGGCAGGTTCACTCACCGCACGGGCTACACACCTGGCCCACGAAGCGCAGCTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGATATGGACTCCACCGCCATCCAGa                           <  1:795143/140‑1 (MQ=255)

                                                                                                         cTGGCAGGTTCACTCACCGCACGGGCTACACACCTGGCCCACGAAGCGCAGCTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGATATGGACTCCACCGCCATCCAGATTGa                       <  1:16980/140‑1 (MQ=255)

                                                                                                           ggCAGGTTCACTCACCGCACGGGCTACACACCTGGCCCACGAAGCGCAGCTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGATATGGACTCCACCGCCATCCAGATTGAAt                     <  2:398278/140‑1 (MQ=255)

                                                                                                                           gCACGGGCTACACACCTGGCCCACGAAGCGCAGCTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGATATGGACTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGc     >  1:485370/1‑140 (MQ=255)

                                                                                                                              cGGGCTACACACCTGGCCCACGAAGCGCAGCTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGATATGGACTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCaa  <  1:573059/140‑1 (MQ=255)

GGTCGTGGGCTGGATAAACAACGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACACGCCTGGCCCACGAAGCGCAGCTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGATATGGACTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAA  >  NC_000913/4625024‑4625289

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A