Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A18 F60 I1 R1 14 41.7 1431096 98.6% 1411060 138.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,312,772 T→G S74R (AGC→CGC)  alsR ← DNA‑binding transcriptional repressor AlsR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,312,7720TG98.9% 331.0 / NA 88S74R (AGC→CGC) alsRDNA‑binding transcriptional repressor AlsR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base G (49/38);  minor base C (1/0);  total (50/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGGTGCGTAAAGTGATGTTAAATACCTTATTCACCACATCCTGCGGCGCTTCATCAAAAGCCAACTCGGAAGGCAATACCTGTTCTGACTGAGAAAAATAATCTTCCAGCGCACTGCGTAAGTTACGAAAGCCGCTAAACCCCAGCAGCTTTGATACCTTAACTATCATCGCTTCAGATACCGCCAGAGCTTCTGCGACATCTTTAATTGCGGGTGCACAACTCAGGTTACCGGGTTTGAGTAACCACTCCACGATGCGGCTTTCATTT  >  NC_000913/4312637‑4312905
                                                                                                                                       |                                                                                                                                     
tGGTGCGTAAAGTGATGTTAAATACCTTATTCACCACATCCTGCGGCGCTTCATCAAAAGCCAACTCGGAAGGCAATACCTGTTCTGACTGAGAAAAATAATCTTCCAGCGCACTGCGTAAGTTACGAAAGCCGCGAAAc                                                                                                                                   <  2:383375/140‑1 (MQ=255)
 ggTGCGTAAAGTGATGTTAAATACCTTATTCACCACATCCTGCGGCGCTTCATCAAAAGTCAACTCGGAAGGCAATACCTGTTCTGACTGAGAAAAATAATCATCCAGCGTACTCCGCAAGTTAGGAAAGCCGCGAAAcc                                                                                                                                  >  1:285693/1‑140 (MQ=255)
    gCGTAAAGTGATGTTAAATACCTTATTCACCACATCCTGCGGCGCTTCATCAAAAGCCAACTCGGAAGGCAATACCTGTTCTGACTGAGAAAAATAATCTTCCAGCGCACTGCGTAAGTTACGAAAGCCGCGAAACCCca                                                                                                                               >  2:442369/1‑140 (MQ=255)
     cGTAAAGTGATGTTAAATACCTTATTCACCACATCCTGCGGCGCTTCATCAAAAGCCAACTCGGAAGGCAATACCTGTTCTGACTGAGAAAAATAATCTTCCAGCGCACTGCGTAAGTTACGAAAGCCGCGAAACCCcag                                                                                                                              >  1:620048/1‑140 (MQ=255)
     cGTAAAGTGATGTTAAATACCTTATTCACCACATCCTGCGGCGCTTCATCAAAAGCCAACTCGGAAGGCAATACCTGTTCTGACTGAGAAAAATAATCTTCCAGCGCACTGCGTAAGATACGAAGGCCGCGAAACCCcag                                                                                                                              >  1:324193/1‑140 (MQ=255)
      gTAAAGTGATGTTAAATACCTTATTCACCACATCCTGCGGCGCTTCATCAAAAGCCAACTCGGAAGGCAATACCTGTTCTGACTGAGAAAAATAATCTTCCAGCGCACTGCGTAAGTTACGAAAGCCGCGAAACCCcagc                                                                                                                             <  2:620048/140‑1 (MQ=255)
      gTAAAGTGATGTTAAATACCTTATTCACCACATCCTGCGGCGCTTCATCAAAAGCCAACTCGGAAGGCAATACCTGTTCTGACTGAGAAAAATAATCTTCCAGCGCACTGCGTAAGTTACGAAAGCCGCGAAACCCcagc                                                                                                                             <  2:349125/140‑1 (MQ=255)
      gTAAAGTGATGTTAAATACCTTATTCACCACATCCTGCGGCGCTTCATCAAAAGCCAACTCGGAAGGCAATACCTGTTCTGACTGAGAAAAATAATCTTCCAGCGCACTGCGTAAGTTACGAAAGCCGCGAAACCCcagc                                                                                                                             <  1:648291/140‑1 (MQ=255)
      gTAAAGTGATGTTAAATACCTTATTCACCACATCCTGCGGCGCTTCATCAAAAGCCAACTCGGAAGGCAATACCTGTTCTGACTGAGAAAAATAATCTTCCAGCGCACTGCGTAAGTTACGAAAGCCGCGAAACCCcagc                                                                                                                             <  2:587929/140‑1 (MQ=255)
      gTAAAGTGATGTTAAATACCTTATTCACCACATCCTGCGGCGCTTCATCAAAAGCCAACTCGGAAGGCAATACCTGTTCTGACTGAGAAAAATAATCTTCCAGCGCACTGCGTAAGTTACGAAAGCCGCGAAACCCcagc                                                                                                                             <  2:511306/140‑1 (MQ=255)
        aaaGTGATGTTAAATACCTTATTCACCACATCCTGCGGCGCTTCATCAAAAGCCAACTCGGAAGGCAATACCTGTTCTGACTGAGAAAAATAATCTTCCAGCGCACTGCGTAAGTTACGAAAGCCGCGAAACCCcagcag                                                                                                                           <  2:681103/140‑1 (MQ=255)
             gATGTTAAATACCTTATTCACCACATCCTGCGGCGCTTCATCAAAAGCCAACTCGGAAGGCAATACCTGTTCTGACTGAGAAAAATAATCTTCCAGCGCACTGCGTAAGTTACGAAAGCCGCGAAACCCCAGCAGCTTTg                                                                                                                      <  1:711578/140‑1 (MQ=255)
             gATGTTAAATACCTTATTCACCACATCCTGCGGCGCTTCATCAAAAGCCAACTCGGAAGGCAATACCTGTTCTGACTGAGAAAAATAATCTTCCAGCGCACTGCGTAAGTTACGAAAGCCGCGAAACCCCAGCAGCTTTg                                                                                                                      <  1:172905/140‑1 (MQ=255)
              aTGTTAAATACCTTATTCACCACATCCTGCGGCGCTTCATCAAAAGCCAACTCGGAAGGCAATACCTGTTCTGACTGAGAAAAATAATCTTCCAGCGCACTGCGTAAGTTACGAAAGCCGCGAAACCCCAGCAGCTTTGa                                                                                                                     <  1:111062/140‑1 (MQ=255)
               tGTTAAATACCTTATTCACCACATCCTGCGGCGCTTCATCAAAAGCCAACTCGGAAGGCAATACCTGTTCTGACTGAGAAAAATAATCTTCCAGCGCACTGCGTAAGTTACGAAAGCCGCGAAACCCCAGCAGCTTTGAt                                                                                                                    >  1:638096/1‑140 (MQ=255)
               tGTTAAATACCTTATTCACCACATCCTGCGGCGCTCCATAAAAAGCCAGCTCGGAAGGCAATACCTGTTCTGACTGAGAAAAATAATCTTCCAGCGCACTGCGTAAGTTACGAAAGCCGCGAAACCCCAGCAGCTTTGAt                                                                                                                    <  2:261299/140‑1 (MQ=255)
                 ttAAATACCTTATTCACCACATCCTGCTGCGCTTCATCAAAAGCCAACTCGGAAGGCAATACCTGTTCTGACTGAGAAAAATAATCTTCCAGCGCACTGCGTAAGTTACGAAAGCCGCGAAACCCCAGCAGCTTTGATAc                                                                                                                  >  1:695647/1‑140 (MQ=255)
                    aaTACCTTATTCACCACATCCTGCGGCGCTTCATCAAAAGCCAACTCGGAAGGCAATACCTGTTCTGACTGAGAAAAATAATCTTCCAGCGCACTGCGTAAGTTACGAAAGCCGCGAAACCCCAGCAGCTTTGATACCtt                                                                                                               >  2:586267/1‑140 (MQ=255)
                     aTACCTTATTCACCACATCCTGCGGCGCTTCATCAAAAGCCAACTCGGAAGGCAATACCTGTTCTGACTGAGAAAAATAATCTTCCAGCGCACTGCGTAAGTTACGAAAGCCGCGAAACCCCAGCAGCTTTGa                                                                                                                     <  1:194665/133‑1 (MQ=255)
                     aTACCTTATTCACCACATCCTGCGGCGCTTCATCAAAAGCCAACTCGGAAGGCAATACCTGTTCTGACTGAGAAAAATAATCTTCCAGCGCACTGCGTAAGTTACGAAAGCCGCGAAACCCCAGCAGCTTTGa                                                                                                                     >  2:194665/1‑133 (MQ=255)
                      tACCTTATTCACCACATCCTGCGGCGCTTCATCAAAAGCCAACTCGGAAGGCAATACCTGTTCTGACTGAGAAAAATAATCTTCCAGCGCACTGCGTAAGTTACGAAAGCCGCGAAACCCCAGCAGCTTTGATACCTTaa                                                                                                             >  1:40037/1‑140 (MQ=255)
                           tATTCACCACATCCTGCGGCGCTTCATCAAAAGCCAACTCGGAAGGCAATACCTGTTCTGACTGAGAAAAATAATCTTCCAGCGCACTGCGTAAGTTACGAAAGCCGCGAAACCCCAGCAGCTTTGATACCTTAACTatc                                                                                                        >  1:396251/1‑140 (MQ=255)
                           tATTCACCACATCCTGCGGCGCTTCATCAAAAGCCAACTCGGAAGGCAATACCTGTTCTGACTGAGAAAAATAATCTTCCAGCGCACTGCGTAAGTTACGAAAGCCGCGAAACCCCAGCAGCTTTGATACCTTAACTatc                                                                                                        >  1:71931/1‑140 (MQ=255)
                           tATTCACCACATCCTGCGGCGCTTCATCAAAAGCCAACTCGGAAGGCAATACCTGTTCTGACTGAGAAAAATAATCTTCCAGCGCACTGCGTAAGTTACGAAAGCCGCGAAACCCCAGCAGCTTTGATACCTTAACTatc                                                                                                        >  1:448035/1‑140 (MQ=255)
                           tATTCACCACATCCTGCGGCGCTTCATCAAAAGCCAACTCGGAAGGCAATACCTGTTCTGACTGAGAAAAATAATCTTCCAGCGCACTGCGTAAGTTACGAAAGCCGCGAAACCCCAGCAGCTTTGATACCTTAACTatc                                                                                                        >  1:142303/1‑140 (MQ=255)
                           tATTCACCACATCCTGCGGCGCTTCATCAAAAGCCAACTCGGAAGGCAATACCTGTTCTGACTGAGAAAAATAATCTTCCAGCGCACTGCGTAAGTTACGAAAGCCGCGAAACCCCAACAGCTTTGATACCTTAACTatc                                                                                                        >  1:322013/1‑140 (MQ=255)
                            aTTCACCACATCCTGCGGCGCTTCATCAAAAGCCAACTCGGAAGGCAATACCTGTTCTGACTGAGAAAAATAATCTTCCAGCGCACTGCGTAAGTTACGAAAGCCGCGAAACCCCAGCAGCTTTGATACCTTAACTatca                                                                                                       <  1:706312/140‑1 (MQ=255)
                            aTTCACCACATCCTGCCGCGCTACATCAAAAGCAACCTCAGAAGGCAACACCTGTTCTGACTGAGAAAAATAATCTTCCAGCGCACTGCGTAAGTTACGAAAGCCGCGAAACCCCAGCAGCTTTGATACCTTAACTatca                                                                                                       <  2:324193/140‑1 (MQ=255)
                             ttCACCACATCCTGCGGCGCTTCATCAAAAGCCAACTCGGAAGGCAATACCTGTTCTGACTGAGAAAAATAATCTTCCAGCGCACTGCGTAAGTTACGAAAGCCGCGAAACCCCAGCAGCTTTGATACCTTAACTatcat                                                                                                      >  2:486772/1‑140 (MQ=255)
                             ttCACCACATCCTGCGGCGCTTCATCAAAAGCCAACTCGGAAGGCAATACCTGTTCTGACTGAGAAAAATAATCTTCCAGCGCACTGCGTAAGTTACGAAAGCCGCGAAACCCCAGCAGCTTTGATACCTTAACTatcat                                                                                                      <  1:586267/140‑1 (MQ=255)
                               caccacATCCTGCGGCGCTTCATCAAAAGCCAACTCGGAAGGCAATACCTGTTCTGACTGAGAAAAATAATCTTCCAGCGCACTGCGTAAGTTACGAAAGCCGCGAAACCCCAGCAGCTTTGATACCTTAACTATCATCg                                                                                                    <  1:582684/140‑1 (MQ=255)
                                  cacaTCCTGCGGCGCTTCATCAAAAGCCAACTCGGAAGGCAATACCTGTTCTGACTGAGAAAAATAATCTTCCAGCGCACTGCGTAAGTTACGAAAGCCGCGAAACCCCAGCAGATTAGATACCCTAACTATCGTCGCtt                                                                                                 >  1:232705/1‑140 (MQ=255)
                                     aTCCTGCGGCGCTTCATCAAAAGCCAACTCGGAAGGCAATACCTGTTCTGACTGAGAAAAATAATCTTCCAGCGCACTGCGTAAGTTACGAAAGCCGCGAAACCCCAGCAGCTTTGATACCTTAACTATCATCGCTTCAg                                                                                              >  2:596442/1‑140 (MQ=255)
                                       ccTGCGGCGCTTCATCAAAAGCCAACTCGGAAGGCAATACCTGTTCTGACTGAGAAAAATAATCTTCCAGCGCACTGCGTAAGTTACGAAAGCCGCGAAACCCCAGCAGCTTTGATACCTTAACTATCATCGCTTCAGAt                                                                                            <  1:85119/140‑1 (MQ=255)
                                       ccTGCGGCGCTTCATCAAAAGCCAACTCGGAAGGCAATACCTGTTCTGACTGAGAAAAATAATCTTCCAGCGCACTGCGTAAGTTACGAAAGCCGCGAAACCCCAGCAGCTTTGATACCTTAACTATCATCGCTTCAGAt                                                                                            >  2:690608/1‑140 (MQ=255)
                                       ccTGCGGCGCTTCATCAAAAGCCAACTCGGAAGGCAATACCTGTTCTGACTGAGAAAAATAATCTTCCAGCGCACTGCGTAAGTTACGAAAGCCGCGAAACCCCAGCAGCTTTGATACCTTAACTATCATCGCTTCAGAt                                                                                            >  2:85119/1‑140 (MQ=255)
                                            ggcgCTTCATCAAAAGCCAACTCGGAAGGCAATACCTGTTCTGACTGAGAAAAATAATCTTCCAGCGCACTGCGTAAGTTACGAAAGCCGCGAAACCCCAGCAGCTTTGATACCCTAACTATCATCGCTTCAGATACCGc                                                                                       >  1:289697/1‑140 (MQ=255)
                                              cgcTTCATCAAAAGCCAACTCGGAAGGCAATACCTGTTCTGACTGAGAAAAATAATCTTCCAGCGCACTGCGTAAGTTACGAAAGCCGCGAAACCCCAGCAGCTTTGATACCTTAACTATCATCGCTTCAGATACCGCCa                                                                                     >  1:668681/1‑140 (MQ=255)
                                                cTTCATCAAAAGCCAACTCGGAAGGCAATACCTGTTCTGACTGAGAAAAATAATCTTCCAGCGCACTGCGTAAGTTACGAAAGCCGCGAAACCCCAGCAGCTTTGATACCTTAACTATCATCGCTTCAGATACCGCCaga                                                                                   >  2:558989/1‑140 (MQ=255)
                                                         aaGCCAACTCGGAAGGCAATACCTGTTCTGACTGAGAAAAATAATCTTCCAGCGCACTGCGTAAGTTACGAAAGCCGCGAAACCCCAGCAGCTTTGATACCTTAACTATCATCGCTTCAGATACCGCCAGAGCTTCTGCg                                                                          <  2:564811/140‑1 (MQ=255)
                                                         aaGCCAACTCGGAAGGCAATACCTGTTCTGACTGAGAAAAATAATCTTCCAGCGCACTGCGTAAGTTACGAAAGCCGCGAAACCCCAGCAGCTTTGATACCTTAACTATCATCGCTTCAGATACCGCCAGAGCTTCTGCg                                                                          >  1:364733/1‑140 (MQ=255)
                                                         aaGCCAACTCGGAAGGCAATACCTGTTCTGACTGAGAAAAATAATCTTCCAGCGCACTGCGTAAGTTACGAAAGCCGCGAAACCCCAGCAGCTTTGATACCTTAACTATCATCGCTTCAGATACCGCCAGAGCTTCTGCg                                                                          <  2:142303/140‑1 (MQ=255)
                                                          aGCCAACTCGGAAGGCAATACCTGTTCTGACTGAGAAAAATAATCTTCCAGCGGCCTGCGTAAGTTACGAAAACCGCGAAACCCCGGCGGCGTTGATACAGTAATTCTCGTCGCTTCATATACGGCCAGAGGTTCTGCgg                                                                         >  2:337421/1‑139 (MQ=255)
                                                           gCCAACTCGGAAGGCGATACCTGTTCTGATTGAGAAAAATAATCTTCCAGCGCACTGCGTAAGTTACGAAAGCCGCGAAACCCCAGCAGCTTTGATACCTTAACTATCATCGCTTCAGATACCGCCAGAGCTTCTGCGAc                                                                        <  1:337421/140‑1 (MQ=255)
                                                           gCCAACTCGGAAGGCAATACCTGTTCTGACTGAGAAAAATAATCTTCCAGCGCACTGCGTAAGTTACGAAAGCCGCGAAACCCCAGCAGCTTTGATACCTTAACTATCATCGCTTCAGATACCGCCAGAGCTTCTGCGAc                                                                        <  2:668681/140‑1 (MQ=255)
                                                           gCCAACTCGGAAGGCAATACCTGTTCTGACTGAGAAAAATAATCTTCCAGCGCACTGCGTAAGTTACGAAAGCCGCGAAACCCCAGCAGCTTTGATACCTTAACTATCATCGCTTCAGATACCGCCAGAGCTTCTGCGAc                                                                        <  1:596442/140‑1 (MQ=255)
                                                             cAACTCGGAAGGCAATACCTGTTCTGACTGAGAAAAATAATCTTCCAGCGCACTGCGTAAGTTACGAAAGCCGCGAAACCCCAGCAGCTTTGATACCTTAACTATCATCGCTTCAGATACCGCCAGAGCTTCTGCGACAt                                                                      <  1:690608/140‑1 (MQ=255)
                                                                     aaGGCAATACCTGTTCTGACTGAGAAAAATAATCTTCCAGCGCACTGCGTAAGTTACGAAAGCCGCGAAACCCCAGCAGCTTTGATACCTTGACTATCATCGCTTCAGATACCGCCAGAGCTACTGCGACATCTTTAAtt                                                              >  2:237912/1‑140 (MQ=255)
                                                                       ggCAATACCTGTTCTGACTGAGAAAAATAATCTTCTAGCGCACTGCGTAAGTTACGAAAGCCGCGAAACCCCAGCAGCTTTGATACCTTAACTATCATCGCTTCAGATACCGCCAGAGCTTCTGCGACATCTTTAATTGc                                                            <  2:285693/140‑1 (MQ=255)
                                                                       ggCAATACCTGTTCTGACTGAGAAAAATAATCTTCCAGCGCACTGCGTCAGTTACGAAAGCCGCGAAACCCCAGCAGCTTTGATACCTTAACTATCATCGCTTCAGATACCGCCAGAGCTTCTGCGACATCTTTAATTGc                                                            <  2:322013/140‑1 (MQ=255)
                                                                       ggCAATACCTGTTCTGACTGAGAAAAATAATCTTCCAGCGCACTGCGTAAGTTACGAAAGCCGCGAAACCCCAGCAGCTTTGATACCTTAACTATCATCGCTTCAGATACCGCCAGAGCTTCTGCGACATCTTTAATTGc                                                            <  2:71931/140‑1 (MQ=255)
                                                                         cAATACCTGTTCTGACTGAGAAAAACAATATTCCAGCCCACTGCGTAAGTTACGAAAGCCGCGAAACCCCAGCAGCTTTGATACCTTAACTATCATCGCTTCAGATACCGCCAGAGCTTCTGCAACATCTTTAATTGCgg                                                          <  1:300853/140‑1 (MQ=255)
                                                                              ccTGTTCTGACTGAGAAAAATAATCTTCCAGCGCACTGCGTAAGTTACGAAAGCCGCGAAACCCCAGCAGCTTTGATACCTTAACTATCATCGCTTCAGATACCGCCAGAGCTTCTGCGACATCTTTAATTGCGGGTGca                                                     <  2:612426/140‑1 (MQ=255)
                                                                                  ttCTGACTGAGAAAAATAATCTTCCAGCGCACTGCGTAAGTTACGAAAGCCGCGAAACCCCAGCAGCTTTGATACCTTAACTATCATCGCTTCAGATACCGCCAGAGCTTCTGCGACATCTTTAATTGCGGGTGCACAAc                                                 >  1:434511/1‑140 (MQ=255)
                                                                                    ctgactgaGAAAAATAATCTTCCAGCGCACTGCGTAAGTTACGAAAGCCGCGAAACCCCAGCAGCTTTGATACCTTAACTATCATCGCTTCAGATACCGCCAGAGCTTCTGCGACATTTTTAATTGCGGGTGCACAACTc                                               >  2:304549/1‑140 (MQ=255)
                                                                                    ctgactgaGAAAAATAATCTTCCAGCGCACTGCGTAAGTTACGAAAGCCGCGAAACCCCAGCAGCTTTGATACCTTAACTATCATCGCTTCAGATACCGCCAGAGCTTCTGCGACATCTTTAAt                                                               <  1:458306/124‑1 (MQ=255)
                                                                                    ctgactgaGAAAAATAATCTTCCAGCGCACTGCGTAAGTTACGAAAGCCGCGAAACCCCAGCAGCTTTGATACCTTAACTATCATCGCTTCAGATACCGCCAGAGCTTCTGCGACATCTTTAAt                                                               >  2:458306/1‑124 (MQ=255)
                                                                                      gactgaGAAAAATAATCTTCCAGCGCACTGCGTAAGTTACGAAAGCCGCGAAACCCCAGCAGCTTTGATACCTTAACTATCATCGCTTCAGATACCGCCAGAGCTTCTGCGACATCTTTAATTGCGGGTGCACAACTCAg                                             >  1:10707/1‑140 (MQ=255)
                                                                                      gactgaGAAAAATAATCTTCCAGCGCACTGCGTAAGTTACGAAAGCCGCGAAACCCCAGCAGCTTTGATACCTTAACTATCATCGCTTCAGATACCGCCAGAGCTTCTGCGACATCTTTAATTGCGGGTGCACAACTCAg                                             >  2:177473/1‑140 (MQ=255)
                                                                                      gactgaGAAAAATAATCTTCCAGCGCACTGCGTAAGTTACGAAAGCCGCGAAACCCCAGCAGCTTTGATACCTTAACTATCATCGCTTCAGATACCGCCAGAGCTTCTGCGACATCTTTAATTGCGGGTGCACAACTCAg                                             >  1:591916/1‑140 (MQ=255)
                                                                                       actgaGAAAAATAATCTTCCAGCGCACTGCGTAAGTTACGAAAGCCGCGAAACCCCAGCAGCTTTGATACCTTAACTATCATCGCTTCAGATACCGCCAGAGCTTCTGCGACATCTTTAATTGCGGGTGCACAACTCAgg                                            >  1:608277/1‑140 (MQ=255)
                                                                                       actgaGAAAAATAATCTTCCAGCGCACTGCGTAAGTTACGAAAGCCGCGAAACCCCAGCAGCTTTGATACCTTAACTATCATCGCTTCAGATACCGCCAGAGCTTCTGCGACATCTTTAATTGCGGGTGCACAACTCAgg                                            >  1:638885/1‑140 (MQ=255)
                                                                                       actgaGAAAAATAATCTTCCAGCGCACTGCGTAAGTTACGAAAGCCGCGAAACCCCAGCAGCTTTGATACCTTAACTATCATCGCTTCAGAAACCGCCAGAGCTGCTGGGACATCTTTAATTACGGGTGCACAACTCAgg                                            >  2:201869/1‑140 (MQ=255)
                                                                                          gagaAAAATAATCTTCCGGCGCTCTGCGTAACTTAGGAATGCCGCGAATCCCCAGCAGCTTTGATACCTTAACTATCATCGCTTCAGATACCGCCAGAGCTTCTGCGACATCTTTAATTGCGGGTGCACAACTCAGGTTa                                         <  2:289697/140‑1 (MQ=255)
                                                                                            gaAAAATAATCTTCCAGCGCACTGCGTAAGTTACGAAAGCCGCGAAACCCCAGCAGCTTTGATACCTTAACTATCATCGCTTCAGATACCGCCAGAGCTTCTTCGACATCATTATTTCCGGGTGCACAACTCAGGTTAcc                                       >  1:254044/1‑140 (MQ=255)
                                                                                            gaAAAATAATCTTCCAGCGCACTGCGTAAGTTACGAAAGCCGCGAAACCCCAGCAGCTTTGATACCTTAACTATCATCGCTTCAGATACCGCCAGAGCTTCTGCGACATCTTTAATTGCGGGTGCACAACTCAGGTTAcc                                       >  1:444375/1‑140 (MQ=255)
                                                                                                aataatCTTCCAGCGCACTGCGTAAGTTACGAAAGCCGCGAAACCCCAGCAGCTTTGATACCTTAACTATCATCGCTTCAGATACCGCCAGAGCTTCTGCGACATCTTTAATTGCGGGTGCACAACTCAGGTTACCGGGt                                   <  1:485363/140‑1 (MQ=255)
                                                                                                 ataatCTTCCAGCGCACTGCGTAAGTTACGAAAGCCGCGAAACCCCAGCAGCTTTGATACCTTAACTATCATCGCTTCAGATACCGCCAGAGCTTCTGCGACATCTTTAATTGCGGGTGCACAACTCAGGTTACCGGGtt                                  <  2:695647/140‑1 (MQ=255)
                                                                                                   aatCTTCCAGCGCACTGCGTAAGTTACGAAAGCCGCGAAACCCCAGCAGCTTTGATACCTTAACTATCATCGCTTCAGATACCGCCAGAGCTTCTGCGACATCTTTAATTGCGGGTGCACAACTCAGGTTACCGGGTTTg                                <  2:638885/140‑1 (MQ=255)
                                                                                                     tCTTCCAGCGCACTGCGTAAGTTACGAAAGCCGCGAAACCCCAGCAGCTTTGATACCTTAACTATCATCGCTTCAGATACCGCCAGAGCTTCTGCGACATCTTTAATTGCGGGTGCACAACTCAGGTTa                                         <  2:662014/129‑1 (MQ=255)
                                                                                                     tCTTCCAGCGCACTGCGTAAGTTACGAAAGCCGCGAAACCCCAGCAGCTTTGATACCTTAACTATCATCGCTTCAGATACCGCCAGAGCTTCTGCGACATCTTTAATTGCGGGTGCACAACTCAGGTTa                                         >  1:662014/1‑129 (MQ=255)
                                                                                                     tCTTCCAGCGCACTGCGTAAGTTACGAAAGCCGCGAAACCCCAGCAGCTTTGATACCTTAACTATCATCGCTTCAGATACCGCCAGAGCTTCTGCGACATCTTTAATTGCGGGTGCACAACTCAGGTTACCGGGTTTGAg                              >  2:712197/1‑140 (MQ=255)
                                                                                                           aGCGCACTGCGTAAGTTGCGAAGGCCGCGAAACTCTAGCGGCTTTGATTCCTTAACTATCATCGCTTCAGATACCGCCAGAGCTTCTGCGACATCTTTAATTGCGGGTGCACAACTCAGGTTACCGGGTTTGAGTAACCa                        <  2:332023/140‑1 (MQ=255)
                                                                                                             cgcACTGCGTAAGTTACGAAAGCCGCGAAACCCCAGCAGCTTTGATACCTTAACTATTATCGCTTCAGATACCGCCAGAGCTTCTGCGACATCTTTAATTGCGGGTGCACAACTCAGGTTACCGGGTTTGAGTAACCACt                      <  2:434511/140‑1 (MQ=255)
                                                                                                             cgcACTGCGTAAGTTACGAAAGCCGCGAAACCCCAGCAGCTTTGATACCTTAACTATCATCGCTTCAGATACCGCCAGAGCTTCTGCGACATCTTTAATTGCGGGTGCACAACTCAGGTTACCGGGTTTGAGTAACCACt                      >  2:10678/1‑140 (MQ=255)
                                                                                                               cACTGCGTAAGTTACGAAAGCCGCGAAACCCCAGCAGCTTTGATACCTTAACTATCATCGCTTCAGATACCGCCAGAGCTTCTGCGACATCTTTAATTGCGGGTGCACAACTCAGGTTACCGGGTTTGAGTAACCACTcc                    >  1:145616/1‑140 (MQ=255)
                                                                                                               cACTGCGTAAGTTACGAAAGCCGCCAAACCCCAGCAGCTTTGATACCTTAACTATCATCGCTTCAGATACCGCCAGAGCTTCTGCGACATCTTTAATTGCGGGTGCACAACTCAGGTTACCGGGTTTGAGTAACCACTcc                    >  1:629412/1‑140 (MQ=255)
                                                                                                                aCTGCGTAAGTTACGAAAGCCGCGAAACCCCAGCAGCTTTGATACCTTAACTATCATCGCTTCAGATACCGCCAGAGCTTCTGCGACATCTTTAATTGCGGGTGCACAACTCAGGTTACCGGGTTTGAGTAACCACTCCa                   <  1:442369/140‑1 (MQ=255)
                                                                                                                         gTTACGAAAGCCGCGAAACCCCAGCAGCTTTGATACCTTAACTATCATCGCTTCAGATACCGCCAGAGCTTCTGCGACATCTTTAATTGCGGGTGCACAACTCACGTTCCTGGGTTTGAGTACCCACTCCACGATGCGGc          >  2:242558/1‑140 (MQ=255)
                                                                                                                         gTTACGAAAGCCGCGAAACCCCAGCAGCTTTGATACCTTAACTATCATCGCTTCAGATACCGCCAGAGCTTCTGAGACATCTTTAATTGCGGGTGCACAACTCAGGTTACCGGGTTTGAGTAACCACTCCACGATGCGGc          >  2:507047/1‑140 (MQ=255)
                                                                                                                            aCGAAAGCCGCGAAACCCCAGCAGCTTTGATACCTTAACTATCATCGCTTCAGATACCGCCAGAGCTTCTGCGACATCTTTAATTGCGGGTGCACAACTCAGGTTACCGGGTTTGAGTAACCACTCCACGATGCGGCttt       >  1:354386/1‑140 (MQ=255)
                                                                                                                            aCGAAAGCCGCGAAACCCCAGCAGCTTTGATACCTTAACTATCATCGCTTCAGATACCGCCAGAGCTTCTGCGACATCTTTAATTGCGGGTGCACAACTCAGGTTACCGGGTTTGAGTAACCACTCCACGATGCGGCttt       >  1:411325/1‑140 (MQ=255)
                                                                                                                            aCGAAAGCCGCGAAACCCCAGCAGCTTTGATACCTTAACTATCATCGCTTCAGATACCGCCAGAGCTTCTGCGACATCTTTAATTGCGGGTGCACAACTCAGGTTACCGGGTTTGAGTAACCACTCCACGATGCGGCttt       >  1:690954/1‑140 (MQ=255)
                                                                                                                              gAAAGCCGCGAAACCCCAGCAGCTTTGATACCTTAACTATCATCGCTTCAGATACCGCCAGAGCTTCTGCGACATCTTTAATTGCGGGTGCACAACTCAGGTTACCGGGTTTGAGTAACCACTCCACGATGCGGCTTTCa     >  1:611670/1‑140 (MQ=255)
                                                                                                                              gAAAGCCGCGAAACCCCAGCAGCTTTGATACCTTAACTATCATCGCTTCAGATACCGCCAGAGCTTCTGCGACATCTTTAATTGCGGGTGCACAACTCAGGTTACCGGGTTTGAGTAACCACTCCACGATGCGGCTTTCa     >  1:153723/1‑140 (MQ=255)
                                                                                                                              gAAAGCCGCGAAACCCCAGCAGCTTTGATACCTTAACTATCATCGCTTCAGATACCGCCAGAGCTTCTGCGACATCTTTAATTGCGGGTGCACAACTCAGGTTACCGGGTTTGAGTAACCACTCCACGATGCGGCTTTCa     >  1:366794/1‑140 (MQ=255)
                                                                                                                              gAAAGCCGCGAAACCCCAGCAGCTTTGATACCTTAACTATCATCGCTTCAGATACCGCCAGAGCTTCTGCGACATCTTTAATTGCGGGTGCACAACTCAGGTTACCGGGTTTGAGTAACCACTCCACGATGCGACTTTCa     >  1:103622/1‑140 (MQ=255)
                                                                                                                              gAAAGCCGCGAAACCCCAGCAGCTTTGATACCTTAACTATCATCGCTTCAGATACCGCCAGAGCTTCTGCGACATCTTTAATTGCGGGTGCACAACTCAGGTGACCGGGTTTGAGTACCCACTCTACGATGCGGCTTTCa     >  1:274535/1‑140 (MQ=255)
                                                                                                                                 aGCCGCGAAACCCCAGCAGCTTTGATACCTTAACTATCATCGCTTCAGATACCGCCAGAGCTTCTGCGACATCTTTAATTGCGGGTGCACAACTCAGGTTACCGGGTTTGAGTAACCACTCCACGATGCGGCTTTCAttt  <  2:145616/140‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                     
TGGTGCGTAAAGTGATGTTAAATACCTTATTCACCACATCCTGCGGCGCTTCATCAAAAGCCAACTCGGAAGGCAATACCTGTTCTGACTGAGAAAAATAATCTTCCAGCGCACTGCGTAAGTTACGAAAGCCGCTAAACCCCAGCAGCTTTGATACCTTAACTATCATCGCTTCAGATACCGCCAGAGCTTCTGCGACATCTTTAATTGCGGGTGCACAACTCAGGTTACCGGGTTTGAGTAACCACTCCACGATGCGGCTTTCATTT  >  NC_000913/4312637‑4312905

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A