Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A17 F60 I1 R1	10	26.5	909534	98.6%	896800	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,616,374	T→C	Y231C (TAC→TGC)	yjjI ←	DUF3029 domain‑containing protein YjjI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,616,374	0	T	C	100.0%	105.0 / NA	29	Y231C (TAC→TGC)	yjjI	DUF3029 domain‑containing protein YjjI
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (15/14); total (15/14)

TAGAGTGCGCGTAAAGAAGTCATCCAGCGATTCGCTGCGCTCGGCAATGGCTTTCAGGTTAAGGCGTACCAGCGTGCTGCCACCACCCGCCAGCGGCAGTGAGTTGTAACAGCTCACAATCCCGTAGCCCCCTTTTGTGAAAATTTTATCATGCACCGGACCGTTGGCGATGTGCGGTTTGCTACATTCACAGATGTTCTTCGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGATCGTAGATAAA  >  NC_000913/4616250‑4616501

tAGAGTGCGCGTAAAGAAGTCATCCAGCGATTCGCTGCGCTCGGCAATGGCTTTCAGGTTAAGGCGTACCAGCGTGCTGCCACCACCCGCCAGCGGCAGTGAGTTGTAACAGCTCACAATCCCGCAGCCCCCTTTTGTGa                                                                                                                  <  2:426521/140‑1 (MQ=255)

     ggcgcGTAAAGAAGTCATCCAGTGATTCGCTTCGCTCGGAAATGGCTTACAGGTTAAGGCGTACCAGCGTGCTGCCACCACCCGCCAGCGGCAGTGAGTTGTAACAGCTCACAATCCCGCAGCCCCCTTTTGTGAAAAtt                                                                                                             <  2:168855/139‑1 (MQ=255)

         cgTAAAGAAGTCATCCAGCGATTCGCTGCGCTCGGCAATGGCTTTCAGGTTAAGGCGTACCAGCGTGCTGCCACCACCCGCCAGCGGCAGTGAGTTGTAACAGCTCACAATCCCGCAGCCCCCTTTTGTGAAAATTTTAt                                                                                                         >  1:339744/1‑140 (MQ=255)

               gaagTCATCCAGCGATTCGCTGCGCTCGGCAATGGCTTTCAGGTTAAGGCGTACCAGCGTGCTGCCACCACCCGCCAGCGGCAGTGAGTTGTAACAGCTCACAATCCCGCAGCCCCCTTTTGTGAAAATTTTATCATGCa                                                                                                   >  2:92622/1‑140 (MQ=255)

                aagTCATCCAGCGATTCGCTGCGCTCGGCAATGGCTTTCAGGTTAAGGCGTACCAGCGTGCTGCCACCACCCGCCAGCGGCAGTGAGTTGTAACAGCTCACAATCCCGCAGCCCCCTTTTGTGAAAATTTTATCATGCAc                                                                                                  <  2:339744/140‑1 (MQ=255)

                   tCATCCAGCGATTCGCTGCGCTCGGCAATGGCTTTCAGGTTAAGGCGTACCAGCGTGCTGCCACCACCCGCCAGCGGCAGTGAGTTGTAACAGCTCACAATCCCGCAGCCCCCTTTTGTGAAAATTTTATCATGCACCgg                                                                                               <  1:92622/140‑1 (MQ=255)

                             aTTCGCTGCGCTCGGCAATGGCTTTCAGGTTAAGGCGTACCAGCGTGCTGCCACCACCCGCCAGCGGCAGTGAGTTGTAACAGCTCACAATGCCGCAGCCTCCTATTGGGAAAATTTAATCAGGCACCGGACCGTTGGCg                                                                                     >  2:137690/1‑140 (MQ=255)

                                  cTGCGCTCGGCAATGGCTTTCAGGTTAAGGCGTACCAGCGTGCTGCCACCACCGGCCAGCGGCAGTGAGTTGTAACAGCTCACAATCCCGCAGCCCCCTTTTGTGAAAATTTTATCATGCACCGGACCGTTGGCGAtgtg                                                                                <  1:250510/140‑1 (MQ=255)

                                      gcTCGGCAATGGCTTTCAGGTTAAGGCGTACCAGCGTGCTGCCACCACCCGCCAGCGGCAGTGAGTTGTAACAGCTCACAATCCCGCAGCCCCCTTTTGTGAAAATTTTATCATGCACCGGACCGTTGGCGATGTGCGGt                                                                            <  1:15469/140‑1 (MQ=255)

                                                    ttCAGGTTAAGGCGTACCAGCGTGCTGCCACCACCCGCCAGCGGCAGTGAGTTGTAACAGCTCACAATCCCGCAGCTCCCTTTTGTGAAAATTTTATCATGCACCGGACCGTTGGCGATGTGCGGTTTGCTACAtt                                                                  >  2:386365/1‑136 (MQ=255)

                                                    ttCAGGTTAAGGCGTACCAGCGTGCTGCCACCACCCGCCAGCGGCAGTGAGTTGTAACAGCTCACAATCCCGCAGCTCCCTTTTGTGAAAATTTTATCATGCACCGGACCGTTGGCGATGTGCGGTTTGCTACAtt                                                                  <  1:386365/136‑1 (MQ=255)

                                                           tAAGGCGTACCAGCGAGCTGCCACCTCCCCCCAGCGGCAGTGAGTTGTAACAGCTCACAATCCCGCAGCCCCCTTTTGTGAAAATTTTATCATGCACCGGACCGTTGGCGATGTGCGGTTTGCTACATTCACAGATGttc                                                       <  1:137690/140‑1 (MQ=255)

                                                            aaGGCGTACCAGCGTGCTGCCACCACCCGCCAGCGGCAGTGAGTTGTAACAGCTCACAATCCCGCAGCCCCCTTTTGTGAAAATTTTATCATGCACCGGACCGTTGGCGATGTGCGGTTTGCTACATTCACAGATGttct                                                      >  2:225747/1‑140 (MQ=255)

                                                                   aCCAGCGTGCTGCCACCACCCGCCAGCGGCAGTGAGTTGTAACAGCTCACAATCCCGCAGCCCCCTTTTGTGAAAATTTTATCATGCACCGGACCGTTGGGGATGTGCAGTTTGCTAGATTCACAGATGTTCTTCGCCAc                                               >  2:141900/1‑140 (MQ=255)

                                                                   aCCAGCGTGCTGCCACCACCCGCCAGCGGCAGTGAGTTGTAACAGCTCACAATCCCGCAGCCCCCTTTTGTGAAAATTTTATCATGCACCGGACCGTTGGCG‑TGTGCGGTTTGCTACATTCACAGATGTTCTTCGCCACt                                              >  2:223804/1‑140 (MQ=255)

                                                                          tgctgcCACCACCCGCCAGCGGCAGTGAGTTGTAACAGCTCACAATCCCGCAGCCCCCTTTTGTGAAAATTTTATCATGCACCGGACCGTTGGCGATGTGCGATTTGCTATATTAACGGATGTTCTTCGCCACTTCcagc                                        >  1:134719/1‑140 (MQ=255)

                                                                            ctgcCACCACCCGCCAGCGGCAGTGAGTTGTAACAGCTCACAATCCCGCAGCCCCCTTTTGTGAAAATTTTATCATGCACCGGACCGTTGGCGATGTGCGGTTTGCTACATTCACAGATGTTCTTCGCCACTTCcagcag                                      >  1:253894/1‑140 (MQ=255)

                                                                                      ccGCCAGCGGCAGTGCGTTGTACCATCTCCCAATCCCGCAGCCCCCTTTTGTGAAAATTTTATCATGCACCGGACCGTTGGCGATGTGCGGTTTGCTACATTCACAGATGTTCTTCGCCACTTCCAGCAGCAGGtcatca                            <  1:141900/140‑1 (MQ=255)

                                                                                      ccGCCAGCGGCAGTGAGTTGTAACAGCTCACAATCCCGCAGCCCCCTTTTGTGAAAATTTTATCATGCACCGGACCGTTGGCGATGTGCGGTTTGCTACATTCACAGATGTTCTTCGCCACTTCCAGCAGCAGGtcatca                            >  2:427637/1‑140 (MQ=255)

                                                                                         ccAGCGGCAGTGAGTTGTAACAGCTCACAATCCCGCAGCCCCCTTTTGTGAAAATTTTATCATGCACCGGACCGTTGGCGATGTGCGGTTTGCTACATTCACAGATGTTCTTCGCCACTTCCAGCAGCAGGTCATCAggg                         >  1:41162/1‑140 (MQ=255)

                                                                                         ccAGCGGCAGTGAGTTGTAACAGCTCACAATCCCGCAGCCCCCTTTTGTGAAAATTTTATCATGCACCGGACCGTTGGCGATGTGCGGTTTACTACATTCACAGCTGTACTTCGCCACTTCCCGCAGCAGGTCATCAggg                         >  2:161664/1‑140 (MQ=255)

                                                                                           aGCGGCAGTGAGTTGTAACAGCTCACAATCCCGCAGCCCCCTTTTGTGAAAATTTTATCATGCACCGGACCGTTGGCGATGTGCGGTTTGCTACATTCACAGATGTTCTTCGCCACTTCCAGCAGCAGGTCATCAGGGGt                       <  2:345356/140‑1 (MQ=255)

                                                                                           aGCGGCAGTGAGTTGTAACAGCTCACAATCCCGCAGCCCCCTTTTGTGAAAATTTTATCATGCACCGGACCGTTGGCGATGTGCGGTTTGCTACATTCACAGATGTTCTTCGCCACTTCCAGCAGCAGGTCATCAGGGGt                       <  1:427637/140‑1 (MQ=255)

                                                                                                  gTGAGTTGTAACAGCTCCCAATTCCGCAGCCGCCTCTTGAGAAAATTTCATCATGCACCGGACCGTTGGCGATGTGCGGTTTGCTACATTCACAGATGTTCTTCGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCa                <  2:134719/140‑1 (MQ=255)

                                                                                                    gAGTTGTAACAGCTCACAATCCCGCAGCCCCCTTTTGTGAAAATTTTATCATGCACCGGACCGTTGGCGATGTGCGGTTTGCTACATTCACAGATGTTCTTCGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAgg              <  2:24654/140‑1 (MQ=255)

                                                                                                       ttGTAACAGCTCACAATACCGCCGCCCCCTTTTGTCAAACTTTTATCATGCACCGGACCGTTGGCGATGTGCGGTTTGCTACATTCACAGATGTTCTTCGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGATc           <  2:163723/140‑1 (MQ=255)

                                                                                                            aCAGCTCACAACCCCGCAGCCCCCTTTTGTGAAAATTTTATCATGCACCGGACCGTTGGCGATGTGCGGTTTGCTACATTCACAGATGTTCTTCGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGATCGTAGa      <  2:41162/140‑1 (MQ=255)

                                                                                                               gCTCACAATCCCGCAGCCCCCTTTTGTGAAAATTTTATCATGCACCGGACCGTTGGCGATGTGCGGTTTGCTACATTCACAGATGTTCTTCGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGATCGTAGATaa   >  2:445874/1‑140 (MQ=255)

                                                                                                                cTCACAATCCCGCAGCCCCCTTTTGTGAAAATTTTATCATGCACCGGACCGTTGGCGATGTGCGGTTTGCTACATTCACAGATGTTCTTCGCCACTTCCAGCGGCAGGTCTTCAGGGTGGATTTGAGGATCGTAGATaaa  >  1:163723/1‑140 (MQ=255)

                                                                                                                cTCACAATCCCGCAGCCCCCTTTTGTGAAAATTTTATCATGCACCGGACCGTTGGCGATGTGCGGTTTGCTACATTCACAGATGTTCTTCGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGATCGTAGATaaa  >  2:380631/1‑140 (MQ=255)

TAGAGTGCGCGTAAAGAAGTCATCCAGCGATTCGCTGCGCTCGGCAATGGCTTTCAGGTTAAGGCGTACCAGCGTGCTGCCACCACCCGCCAGCGGCAGTGAGTTGTAACAGCTCACAATCCCGTAGCCCCCTTTTGTGAAAATTTTATCATGCACCGGACCGTTGGCGATGTGCGGTTTGCTACATTCACAGATGTTCTTCGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGATCGTAGATAAA  >  NC_000913/4616250‑4616501

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A