Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A48 F60 I1 R1	19	49.8	1677902	98.7%	1656089	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,223,955	C→A	A43D (GCC→GAC)	metH →	cobalamin‑dependent methionine synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,223,955	0	C	A	100.0%	218.2 / NA	65	A43D (GCC→GAC)	metH	cobalamin‑dependent methionine synthase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (30/35); total (30/35)

TGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGATCGCCGCTATCCACAACGCCTACTTTGAAGCGGGCGCGGATATCATCGAAACCAACACCTTCAACTCCA  >  NC_000913/4223829‑4224086

tgAGCAGCAAAGTGGCACAACTTCGCGCGAAGTTAAACGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGACGACTGGCCATGc                                                                                                                        <  1:309781/140‑1 (MQ=255)

      gcaAAGTGGAACAACTGCGTGCGCATTTACATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGACGACTGGCCATGCGACCTc                                                                                                                  <  1:359375/140‑1 (MQ=255)

       caAAGTGGAACAACTTCGTGCGCAGTTAAATGAACGTTTTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGACGACTGGCCATGCGACCTCa                                                                                                                 <  1:404127/140‑1 (MQ=255)

       caAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGACGACTGGCCATGCGACCTCa                                                                                                                 >  1:470305/1‑140 (MQ=255)

       caAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTCGAGAACGCTTAGACGACTGGCCATGCGACCTCa                                                                                                                 >  2:359375/1‑140 (MQ=255)

                    cTGCGTGCGCAGTTACATGAACGTACTCTAGTGCTGGCCGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGACGACTGGCCATGCGACCTCAAAGGCAACAACGa                                                                                                    <  1:237450/140‑1 (MQ=255)

                    cTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGACGACTGGCCATGCGACCTCAAAGGCAACAACGa                                                                                                    <  1:693075/140‑1 (MQ=255)

                      gcgtgtgCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGACGACTGGCCATGCGACCTCAAAGGCAACAACGAcc                                                                                                  >  2:651283/1‑140 (MQ=255)

                      gcgtgcgCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGACGACTGGCCATGCGACCTCAAAGGCAACAACGAcc                                                                                                  >  1:654472/1‑140 (MQ=255)

                      gcgtgcgCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGACGACTGGCCATGCGACCTCAAAGGCAACAACGAcc                                                                                                  <  2:503713/140‑1 (MQ=255)

                        gtgcgCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTGGACTACTTGCCATGCGGCCTCAAAGGCAACAACGACctg                                                                                                >  2:237010/1‑140 (MQ=255)

                        gtgcgCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGGGAACGCCTCGACGAATAGCCATGCGACCTCCAAGGCAACAACGACctg                                                                                                >  2:405920/1‑140 (MQ=255)

                         tgcgCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGACGACTGGCCATGCGACCTCAAAGGCAACAACGACctgc                                                                                               <  2:491534/140‑1 (MQ=255)

                         tgcgCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTCAACGCTTTTACGACCGGCCATGCGATCTCATCGGCAACATCGACctgc                                                                                               >  2:409215/1‑140 (MQ=255)

                           cgcAGTTAAATGAACTTATTCTAGTACTGAACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGACGACTGGCCATGCGACCTCAAAGGCAACAACGACctgctg                                                                                             <  1:409215/140‑1 (MQ=255)

                             cAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCTTGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGCGAACGCTTTGACGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGt                                                                                           <  1:126352/140‑1 (MQ=255)

                              aGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGACGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTa                                                                                          >  1:97322/1‑140 (MQ=255)

                               gTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGACGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTAc                                                                                         <  2:646039/140‑1 (MQ=255)

                                   gagacaggTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGACGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAg                                                                                     <  1:333531/133‑1 (MQ=255)

                                        aCGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGACGACTGGCCATGCGACCTCAAAGGCAACAACGAcc                                                                                                  >  2:460591/1‑122 (MQ=255)

                                        aCGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGACGACTGGCCATGCGACCTCAAAGGCAACAACGAcc                                                                                                  <  1:460591/122‑1 (MQ=255)

                                         cGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGACGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAAcc                                                                               >  1:212557/1‑140 (MQ=255)

                                          gTATTCTGGTGCTGGTCGGCGGTATGGGCTCCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCCTTGACGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCg                                                                              <  1:405920/140‑1 (MQ=255)

                                          gTATTCTGGTGCTGGCCGGCGGCATCGGCCCCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGACGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCg                                                                              <  1:237010/140‑1 (MQ=255)

                                                  gTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGACGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGAt                                                                      <  2:654472/140‑1 (MQ=255)

                                                    gCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGACGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGATcg                                                                    <  2:470305/140‑1 (MQ=255)

                                                     cTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGACGACTGGCCATGCGACCTCAAAGGCACCAACGACATGCTGGTAGTCAGGAAACCGGAAgctgtctc                                                                   >  2:333531/1‑133 (MQ=255)

                                                     cTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGACGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAg                                                                                     >  2:158629/1‑122 (MQ=255)

                                                     cTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGACGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAg                                                                                     <  1:158629/122‑1 (MQ=255)

                                                       gggcggctgTATGGGCGCCATGATCTAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGACGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGATcgccg                                                                 <  1:825686/137‑1 (MQ=255)

                                                        gACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCAATTTTCGTGGTGAACGCTTTGACGACTGGCCATGCGACCTCAAAGGCAACATCGAGCTGTTGGTACTCAGTAAACCGGAAGTAATcgccgc                                                                >  2:377971/1‑140 (MQ=255)

                                                             cggTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGACGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCCGGTGATCAGTAACCCGGGCGTGATCGCCGCTATcc                                                           >  2:408952/1‑140 (MQ=255)

                                                                tATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGACGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGATCGCCGCTATCcaca                                                        >  1:816528/1‑140 (MQ=255)

                                                                    ggCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGACGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGATCGCCGCTATCCACAACGc                                                    <  1:144191/140‑1 (MQ=255)

                                                                    ggCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGACGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGATCGCCGCTATCCACAACGc                                                    <  2:97322/140‑1 (MQ=255)

                                                                    ggCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGACGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGATCGCCGCTATCCACAACGc                                                    <  2:212557/140‑1 (MQ=255)

                                                                         cATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGACGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGATCGCCGCTATCCACAACGCCTACt                                               >  1:686231/1‑140 (MQ=255)

                                                                              tCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGACGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGATCGCCGCTATCCACAACGCCTACTTTGaa                                          >  1:650257/1‑140 (MQ=255)

                                                                                cAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGACGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGATCGCCGCTATCCACAACGc                                                    >  2:733427/1‑128 (MQ=255)

                                                                                cAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGACGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGATCGCCGCTATCCACAACGc                                                    <  1:733427/128‑1 (MQ=255)

                                                                                cAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGACGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGATCGCCGCTATCCACAACGCCTACTTTGAAGc                                        <  2:816528/140‑1 (MQ=255)

                                                                                   agTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGACGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGATCGCCGCTATCCACAACGCCTACTTTGAAGCggg                                     >  1:511387/1‑140 (MQ=255)

                                                                                   agTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGACGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGATCGCCGCTATCCACAACGCCTACTTTGAAGCggg                                     >  1:450140/1‑140 (MQ=255)

                                                                                    gTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGACGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGATCGCCGCTATCCACAACGCCTACTTTGAAGCGGgc                                    <  2:686231/140‑1 (MQ=255)

                                                                                     ttATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGACGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGATCGCCGCTATCCACAACGCCTACTTTGAAGCGGgcg                                   >  2:640801/1‑140 (MQ=255)

                                                                                     ttATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGACGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGATCGCCGCTATCCACAACGCCTACTTTGAAGCGGgcg                                   >  1:413784/1‑140 (MQ=255)

                                                                                     ttATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGACGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGATCGCCGCTATACAAAACGCCTACTTTGAAGCGGgcg                                   >  1:294400/1‑140 (MQ=255)

                                                                                       aTCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGACGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGATCGCCGCTATCCACAACGCCTACTTTGAAGCGGGcgcg                                 >  2:728465/1‑140 (MQ=255)

                                                                                        tCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGACGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGATGGCCGCTAGCCAGAAAGCCTACCTTGAAGCGGGCGCgg                                >  1:409143/1‑140 (MQ=255)

                                                                                           aCTGAACGAAGCCGACTTTCGTCGTTAACCCTTTGACAACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGATCGCCGCTATCCACAACGCCTACTTTGAAGCGGGCGCGGata                             <  1:408952/140‑1 (MQ=255)

                                                                                             tGAACGAAGCCGATTTTCGTGGTGAACGCTTTGACGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGATCGCCGCTATCCACAACGCCTACTTTGAAGCGGGCGCGGATatc                           >  1:643065/1‑140 (MQ=255)

                                                                                                aCGAAGCCGATTTTCGTGGTGAACGCTTTGACGACTGGCCATGCGTCCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGATCGCCGCTATCCACAACGCCTACTTTGAAGCGGGCGCGGATatcatc                        <  1:557949/140‑1 (MQ=255)

                                                                                                aCGAAGCCGATTTTCGTGGTGAACGCTTTGACGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGATCGCCGCTATCCACAACGCCTACTTTGAAGCGGGCGCGGATatcatc                        <  1:728465/140‑1 (MQ=255)

                                                                                                 cGAAGCCGATTTTCGTGGTGAATGCTTTGACCACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGATCGCCGCTATCCACAACGCCTACTTTGAAGCGGGCGCGGATATCATCg                       <  2:334567/140‑1 (MQ=255)

                                                                                                  gAAGCCGATTTTCGTGGTGAACGCTTTGACGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGATCGCCGCTATCCACAACGCCTACTTTGAAGCGGGCGCGGATATCATCGa                      >  2:519460/1‑140 (MQ=255)

                                                                                                       caaTTTTCGTGGTGAACGCTTTTACGACTAGCCATGCAACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGATCGCCGCTATCCACAACGCCTACTTTGAAGCGGGCGCGGATATCATCGAAACCa                 <  1:377971/138‑1 (MQ=255)

                                                                                                        gATTTTCGTGGTGAACGCTTTGACGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGATCGCCGCTATCCACAACGCCTACTTTGAAGCGGGCGCGGATATCATCGAAACCaa                <  1:422814/140‑1 (MQ=255)

                                                                                                         aTTTTCGTGGTGAACGCTTTGACGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGATCGCCGCTATCCACAACGCCTACTTTGAAGCGGGCGCGGATATCATCGAAACCAac               <  1:519460/140‑1 (MQ=255)

                                                                                                         aTTTTCGTGGTGAACGCTTTGACGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGATCGCCGCTATCCACAACGCCTACTTTGAAGCGGGCGCGGATATCATCGAAACCAac               >  1:711504/1‑140 (MQ=255)

                                                                                                         aTTTTCGTGGTGAACGCTTTGACGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGATCGCCGCAATCCACAACGCCTACTTTGAAGCGGGCGCGGATATCATCGAAACCAac               >  2:292652/1‑140 (MQ=255)

                                                                                                              cGTGGTGAACGCTTTGACGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGATCGCCGCTATCCACAACGCCTACGTTGAAGCGGGCACGAATATCAGCGAAACCAACACCtt          >  1:227216/1‑140 (MQ=255)

                                                                                                               gtggtgAACGCTTTGACGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGATCGCCGCTATCCACAACGCCTACTTTGAAGCGGGCGCGGATATCATCGAAACCAACACCTTc         <  1:651283/140‑1 (MQ=255)

                                                                                                               gtggtgAACGCTTTGACCACTGCCCATGCGACCTCTAAGTCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGATCGCCGCTATCCACAACGCCTACTTTGAAGCGGGCGCGGATATCATCGAAACCAACACCTTc         <  2:227216/140‑1 (MQ=255)

                                                                                                                 ggtgAACGCTTTGACGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGATCGCCGCTATCCACAACGCCTACTTTGAAGCGGGCGCGGATATCATCGAAACCAACACCTTCaa       <  2:711504/140‑1 (MQ=255)

                                                                                                                     aaCGCTTTGACGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGATCGCCGCTATCCACAACGCCTACTTTGAAGCGGGCGCGGATATCATCGAAACCAACACCTTCAACTcc   >  2:423872/1‑140 (MQ=255)

                                                                                                                      aCGCTTTGACGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGATCGCCGCTATCCACAACGCCTACTTTGAAGCGGGCGCGGATATCATCGAAACCAACACCTTCAACTCCa  <  2:650257/140‑1 (MQ=255)

TGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGATCGCCGCTATCCACAACGCCTACTTTGAAGCGGGCGCGGATATCATCGAAACCAACACCTTCAACTCCA  >  NC_000913/4223829‑4224086

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A