Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A43 F60 I1 R1	21	74.4	2534558	98.9%	2506677	138.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,815,655	+GC	coding (114/642 nt)	pyrE ←	orotate phosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,815,653	1	.	G	94.8%	272.2 / 5.9	77	coding (116/642 nt)	pyrE	orotate phosphoribosyltransferase
Reads supporting (aligned to +/- strand): ref base . (2/2); new base G (38/35); total (40/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.08e-01
*	NC_000913	3,815,653	2	.	C	94.7%	260.4 / 5.6	77	coding (116/642 nt)	pyrE	orotate phosphoribosyltransferase
Reads supporting (aligned to +/- strand): ref base . (2/2); new base C (36/35); total (40/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.83e-01

CCACAGCGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAAC‑‑A‑‑GCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTC  >  NC_000913/3815519‑3815778

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ccACAGCGGTTGTGGTGACAATCGGGATCCCCTTGCAAGTAGGGCCAACCAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAAC‑‑A‑‑Gctcc                                                                                                                          <  2:377677/140‑4 (MQ=255)

    aGCGGTTGTGGTGGCGATCGGGATCCCTTCGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAAC‑‑A‑‑Gcgcccggc                                                                                                                      <  1:462234/140‑8 (MQ=255)

       ggTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAAC‑‑AGCGCCCGGCGtt                                                                                                                     >  2:173003/1‑140 (MQ=255)

        gTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACACTGCTAGATCGCGCCCGTTATTGAAC‑‑AGCGCCCGGCGTTg                                                                                                                    >  2:462234/1‑140 (MQ=255)

          tgtgGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCTGTATTAAAC‑‑AGCGCCCGGCGTTgaa                                                                                                                  >  1:553938/1‑140 (MQ=255)

          ggtggtgGCAATCGGGATCCCTATGTAAGCAAGGCTAAATAGCAGATCAAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAAC‑‑AGCGCCCGGCGTTgaa                                                                                                                  <  2:479481/139‑1 (MQ=255)

          ggtggtgGCAATCGGGAGCCCTGTGTAAGCAAGGCCAAACAGCAGATCAAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAAC‑‑AGCGCCCGGCGTTgaa                                                                                                                  <  2:455919/139‑1 (MQ=255)

              gtggCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAAC‑‑AGCGCCCGGCGTTGAAGaaa                                                                                                              >  2:932580/1‑140 (MQ=255)

              gtggCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAAC‑‑AGCGCCCGGCGTTGAAGaaa                                                                                                              >  2:688943/1‑140 (MQ=255)

              gtggCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAAC‑‑AGCGCCCGGCGTTGAAGaaa                                                                                                              >  2:1192253/1‑140 (MQ=255)

                   aaTCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGTGCCAGGTATTAAAC‑‑AGTGCCCAGCGTTGAAGAAATAggg                                                                                                         >  2:471631/1‑140 (MQ=255)

                   aaTCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAAC‑‑AGCGCCCGGCGTTGAAGAAATAggg                                                                                                         >  2:983635/1‑140 (MQ=255)

                   aaTCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAAC‑‑AGCGCCCGGCGTTGAAGAAATAggg                                                                                                         <  1:932580/140‑1 (MQ=255)

                   aaTCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTACCAGATCGAGCCCGGTCTTAAACAGA‑‑GCCCCGCGTTGAAGAAATAggg                                                                                                         >  2:438080/1‑140 (MQ=255)

                      cGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCTGTATTAAACAGA‑‑GCGCGGCGTAGAAGAAATAGGGGcc                                                                                                      >  1:479481/1‑139 (MQ=255)

                      cGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAATC‑‑AGTGCTCGGCGTGGAAGAAATAGGGGcc                                                                                                      >  1:455919/1‑139 (MQ=255)

                           tCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAAC‑‑AGCGCCCGGCGTTGAAGAAATAGGGGCTTTTgc                                                                                                 <  1:120436/140‑1 (MQ=255)

                             ccTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAAC‑‑AGCGCCCGGCGTTGAAGAAATAGGGGCTTTTgcgc                                                                                               <  1:1134783/140‑1 (MQ=255)

                               tttGTAAGCAGGGCCAATCAGCTGATCGAACACAATGCCGGAATCCACTAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAAC‑‑AGCGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGccc                                                                                             <  2:526141/140‑1 (MQ=255)

                               tttGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAAC‑‑AGCGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGccc                                                                                             >  1:628116/1‑140 (MQ=255)

                                ttGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAAC‑‑AGCGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCg                                                                                            >  2:1077326/1‑140 (MQ=255)

                                   tAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAAC‑‑AGCGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGAt                                                                                         >  1:1090127/1‑140 (MQ=255)

                                     aGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAAC‑‑AGCGCCCGGCGTTGAAGTAACAGGGGCGTTTGCGCCCGGAttt                                                                                       >  1:538526/1‑140 (MQ=255)

                                     aGCAGGGCCAAACAGCAAATCGAACTCAATGCCGGAATCCACCAACGCCTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAAC‑‑AGCGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGAttt                                                                                       <  2:502273/140‑1 (MQ=255)

                                          ggCCAAACAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAAC‑‑AGCGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCg                                                                                  >  1:1075901/1‑140 (MQ=255)

                                          ggCCAAACAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAAC‑‑AGCGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCg                                                                                  >  1:1092605/1‑140 (MQ=255)

                                          ggCCAAACAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAAC‑‑AGCGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCg                                                                                  >  1:1170113/1‑140 (MQ=255)

                                           gCCAAACAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAAC‑‑AGCGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGAttt                                                                                       <  1:131619/134‑1 (MQ=255)

                                           gCCAAACAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAAC‑‑AGCGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGAttt                                                                                       >  2:131619/1‑134 (MQ=255)

                                              aaaCAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAAC‑‑AGCGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTaaa                                                                              <  1:688943/140‑1 (MQ=255)

                                               aaCAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAAC‑‑AGCGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAAc                                                                             >  2:239428/1‑140 (MQ=255)

                                                    cagATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAAC‑‑AGCGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGcc                                                                        >  1:100724/1‑140 (MQ=255)

                                                     gacaggAACTCAATGCTGGAATTCACCAACGCTTCAGCGTAAAAACGGGCTAACAGTGCCAGATCGCGCCCGGTATTAAAC‑‑AGCGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCa                                                                       <  2:555795/135‑1 (MQ=255)

                                                            aCTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAAC‑‑AGCGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCACCAAACTTTa                                                                >  1:622871/1‑140 (MQ=255)

                                                             cTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAAC‑‑AGCGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTaa                                                               <  2:1092605/140‑1 (MQ=255)

                                                               cAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAAC‑‑AGCGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAaca                                                             <  1:1077326/140‑1 (MQ=255)

                                                               cAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAAC‑‑AGCGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAaca                                                             <  1:758704/140‑1 (MQ=255)

                                                               cAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAAC‑‑AGCGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAaca                                                             <  1:951172/140‑1 (MQ=255)

                                                                     cGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAAC‑‑AGCGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCtgtc                                                       >  1:555795/1‑138 (MQ=255)

                                                                       gAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAAC‑‑AGCGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCa                                                                       >  1:1251729/1‑122 (MQ=255)

                                                                       gAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAAC‑‑AGCGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCa                                                                       <  2:1251729/122‑1 (MQ=255)

                                                                        aaTCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAAC‑‑AGCGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCtgcttgc                                                    >  1:951803/1‑140 (MQ=255)

                                                                        aaTCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAAC‑‑AGCGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCtgcttgc                                                    >  2:1124009/1‑140 (MQ=255)

                                                                          tCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAAC‑‑AGCGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTa                                                  >  2:1222302/1‑140 (MQ=255)

                                                                             accaACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAAC‑‑AGCGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAgc                                               >  1:659139/1‑140 (MQ=255)

                                                                              ccaACGCTTCAGCGTCAAAACGACCCAACCGTGCCAGCTCGCGCCCGGTATTAAAC‑‑AGCGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAgcg                                              <  1:335929/140‑1 (MQ=255)

                                                                              ccaACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAAC‑‑AGCGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCGCGGATTTCTGCGCAAAGTCGCCAAACTTTAACACCTGCTTGCTAAgcg                                              >  2:335929/1‑140 (MQ=255)

                                                                               caACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAAC‑‑AGCGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAgcgc                                             <  1:39837/140‑1 (MQ=255)

                                                                               caACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAAC‑‑AGCGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAgcgc                                             <  2:100724/140‑1 (MQ=255)

                                                                               caACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAAC‑‑AGCGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAgcgc                                             <  2:1075901/140‑1 (MQ=255)

                                                                                 aCGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAAC‑‑AGCGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCaa                                           >  1:624205/1‑140 (MQ=255)

                                                                                 aCGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAAC‑‑AGCGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCaa                                           <  1:754871/140‑1 (MQ=255)

                                                                                  cGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAAC‑‑AGCGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCaaa                                          >  1:75049/1‑140 (MQ=255)

                                                                                  cGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAAC‑‑AGCGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCaaa                                          >  2:926340/1‑140 (MQ=255)

                                                                                  cGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAAC‑‑AGCGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACGTTAACACCTACTTGCTAAGCGCaaa                                          >  1:371597/1‑140 (MQ=255)

                                                                                    cTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAAC‑‑AGCGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAAtt                                        >  1:157451/1‑140 (MQ=255)

                                                                                      tCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAAC‑‑AGCGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTTAGCGTAAAATCGTCAAGCTTTAACAGCTGCCTGCTAAGCGCAAATTCa                                      >  2:334238/1‑140 (MQ=255)

                                                                                       cAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAAC‑‑AGCGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCaa                                     >  1:1139058/1‑140 (MQ=255)

                                                                                            tAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAAC‑‑AGCGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAAc                                <  2:75049/140‑1 (MQ=255)

                                                                                               aaaCGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAAC‑‑AGCGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTgg                             <  1:173003/140‑1 (MQ=255)

                                                                                               aaaCGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAAC‑‑AGCGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTgg                             <  1:1192253/140‑1 (MQ=255)

                                                                                               aaaCGGCCTAACAGTACCAGATCGCACCCAGTATTAATC‑‑AGCGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTgg                             <  1:334238/140‑1 (MQ=255)

                                                                                                 aCGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAAC‑‑AGCGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGgcg                           <  2:834509/140‑1 (MQ=255)

                                                                                                 aCGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAAC‑‑AGCGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGgcg                           <  2:1090127/140‑1 (MQ=255)

                                                                                                 aCGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAAC‑‑AGCGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGgcg                           <  2:1139058/140‑1 (MQ=255)

                                                                                                 aCGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAAC‑‑AGCGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGgcg                           <  1:1124009/140‑1 (MQ=255)

                                                                                                 aCGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAAC‑‑AGCGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGgcg                           <  2:1206242/140‑1 (MQ=255)

                                                                                                    gCCTAACAGTGCCAGATCGCGCCCGGTATTAAAC‑‑AGCGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTg                        >  1:1207785/1‑140 (MQ=255)

                                                                                                       tAACAGTGCCAGATCGCGCCCGGTATTAAAC‑‑AGCGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGata                     <  1:865369/140‑1 (MQ=255)

                                                                                                        aaCAGTGCCAGATCGCGCCCGGTATTAAAC‑‑AGCGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGatat                    <  2:157451/140‑1 (MQ=255)

                                                                                                        aaCAGTGCCAGATCGCGCCCGGTATTAAAC‑‑AGCGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGatat                    <  2:951803/140‑1 (MQ=255)

                                                                                                                   aTCGCGCCCGGTATTAAAC‑‑AGCGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGcc         >  2:307733/1‑140 (MQ=255)

                                                                                                                     cgcgCCCGGTATTAAAC‑‑AGCGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCtt       <  1:239428/140‑1 (MQ=255)

                                                                                                                       cgcCCGGTATTAAAC‑‑AGCGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCg     >  1:958705/1‑140 (MQ=255)

                                                                                                                         cccGGTATTAAAC‑‑AGCGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGct   <  1:926340/140‑1 (MQ=255)

                                                                                                                         cccGGTATTAAAC‑‑AGCGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCACCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGct   <  2:622871/140‑1 (MQ=255)

                                                                                                                          ccGGTATTAAAC‑‑AGCGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAAATCAATAATCTGGCGCTGATATAGTTTCATGCCTTCGctc  >  2:549230/1‑140 (MQ=255)

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CCACAGCGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAAC‑‑A‑‑GCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTC  >  NC_000913/3815519‑3815778

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A