Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F60 I1 R1	15	43.4	1487288	98.4%	1463491	138.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,431,626	T→C	T120A (ACC→GCC)	ytfE ←	iron‑sulfur cluster repair protein YtfE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,431,626	0	T	C	95.7%	159.6 / NA	46	T120A (ACC→GCC)	ytfE	iron‑sulfur cluster repair protein YtfE
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (23/21); minor base G (0/2); total (23/23)
Fisher's exact test for biased strand distribution p-value = 4.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGCTTCATCGTGCTCGCTTTCCATTACGCTGATTGGCCCCATTGCCTGGCTGCCCATGCCTTGTTTGATCATCGGGAAGAGGATCTGCTCTTCTTTCATCATGTGGCTGGAAAGCTCTTCATGCAGCATGGTCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGAACGCGCTCGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACGATGATATGGTCGATGAT  >  NC_000913/4431494‑4431761

ccGCTTCATCGTGCTCGCTTTCCATTACGCTGATTGGCCCCATTGCCTGGCTGCCCATGCCTTGTTTGATCATCGGGAAGAGGATCTGCTCTTCTTTCATCATGTGGCTGGAAAGCTCTTCATGCAGCATGGCCAGGTAt                                                                                                                                  <  1:742469/140‑1 (MQ=255)

        tCGTGCTCGCTTTCCATTACGCTGATTGGCCCCATTGCCTGGCTGCCCATGCCTTGTTTGATCATCGGGAAGAGGATCTGCTCTTCTTTCATCATGTGGCTGGAAAGCTCTTCATGCAGCATGGCCAGGTATTTTGTCAg                                                                                                                          <  1:59713/140‑1 (MQ=255)

                                aTTGGCCCCATTGCCTGGCTGCCCATGCCTTGTTTGATCATCGGGAAGAGGATCTGCTCTTCTTTCATCATGTGGCTGGAAAGCTCTTCATGCAGCATGGCCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGTTTGTc                                                                                                  >  2:55333/1‑140 (MQ=255)

                                          ttGCCTGGCTGCCCATGCCTTGTTTGATCATCGGGAAGAGGATCTGCTCTTCTTTCATCATGTGGCTGGAAAGCTCTTCATGCAGCATGGCCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGAAcg                                                                                        >  1:389082/1‑140 (MQ=255)

                                          ttGCATGGCTGCCCATGCCTTGTTTGATCATCGGGAAGAGGATATGCTCTTCTTTCATCATGTGGCTGGAAAGCTCTTCATGCAGCATGGCCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGAAcg                                                                                        <  1:89271/140‑1 (MQ=255)

                                                   tGCCCATGCCTTGTTTGATCATCGGGAAGAGGATCTGCTCTTCTTTCATCATGTGGCTGGAAAGCTCTTCATGCAGCATGGCCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGAACGCGCTCGACt                                                                               >  1:390940/1‑140 (MQ=255)

                                                     cccATGCCTTGTTTGATCATCGGGAAGAGGATCTGCTCTTCTTTCATCATGTGGCTGGAAAGCTCTTCATGCAGCATGGCCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGAACGCGCTCGACttt                                                                             >  2:672224/1‑140 (MQ=255)

                                                       cATGCCTTGTTTGATCATCGGGAAGAGGATCTGCTCTTCTTTCATCATGTGGCTGGAAAGCTCTTCATGCAGCATGGCCAGGTATTTTGTCCGCCCTTTTAGCACGCTTGGTTTGTCGGCGTGAACGCGCTCGACTTTAg                                                                           >  2:300729/1‑140 (MQ=255)

                                                          gCCTTGTTTGATCATCGGGAAGAGGATCTGCTCTTCTTTCATCATGTGGCTGGAAAGCTCTTCATGCAGCATGGCCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGAACGCGCTCGACTTTAGTCg                                                                        <  2:529510/140‑1 (MQ=255)

                                                               gTTTGATCATCGGGAAGAGGATCTGCTCTTCTTTCATCATGTGGCTGGAAAGCTCTTCATGCAGCATGGCCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGTTTTGTCGGCGTAAAGGCGCTCGACTTTAGTCGCTTGc                                                                   >  1:336599/1‑140 (MQ=255)

                                                               gTTTGATCATCGGGAAGAGGATCTGCTCTTCTTTCATCATGTGGCTGGAAAGCTCTTCATGCAGCATGGCCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGTCTGTAGGCGTGAATGCGCTTGACTTTAGTCGCTTGc                                                                   >  2:320242/1‑140 (MQ=255)

                                                               gTTTGATCATCGGGAAGAGGATCTGCTCTTCTTTCATCATGTGGCTGGAAAGCTCTTCATGCAGCATGGCCAGGTATTTTGTCAGCCCTTTTGGCACGCTAGGTCTGTTGGCGTGAAGGCGCGCGACTTTAGTCGCTTGc                                                                   >  2:243944/1‑140 (MQ=255)

                                                                        tcGGGAAGAGGATCTGCTCTTCCTTCATCATGTGGCTTGAAAGCTCTTCATGCAGCATGGCCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGAACGCGCTCGACTTTAGTCGCTTGCAGAATCAGc                                                          <  1:300729/140‑1 (MQ=255)

                                                                          gggAAGAGGATCTGCTCTTCTTTCATCATGTGGCTGGAAAGCTCTTCATGCAGCATGGCCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGAACGCGCTCGACTTTAGTCGCTTGCAGAATCAGCTc                                                        >  1:45422/1‑140 (MQ=255)

                                                                              agagGATCTGCTCTTCTTTCATGATGTGGATGGAAAGATCTTCATGCAGCATGGCCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGAACGCGCTCGACTTTAGTCGCTTGCAGAATCAGCTCCGGc                                                    <  1:320242/140‑1 (MQ=255)

                                                                                   aTCTGCTCTTCTTTCATCATGTGGCTGGAAAGCTCTTCATGCAGCATGGCCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGAACGCGCACGACTTTAGTCGCTGGCAGAAGCAGCTCCGGCAGTTg                                               >  1:193923/1‑140 (MQ=255)

                                                                                    tCTGCTCTTCTTTCATCATGTGGCTGGAAAGATCTTCATACAGCATGGGCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGAACGCGCTCGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGTTGc                                              <  2:312641/140‑1 (MQ=255)

                                                                                       gCTCTTCTTTCATCATGTGGCTGGAAAGCTCTTCATGCAGCATGGCCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGAACGCGCTCGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTcg                                           <  2:389082/140‑1 (MQ=255)

                                                                                          cttcttTCATCATGTGGCTGGAAAGCTCTTCATGCAGCATGGCCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGAACGCGCTCGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCgg                                        >  2:525604/1‑140 (MQ=255)

                                                                                           ttcttTCATCATGTGGCTGGAAAGCTCTTCATGCAGCATGGCCAGGTATTTTGTCAGCCCTTTTGGCCCGCTCGGTTTGTTGGCGTGAACGCGCTCGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGt                                       <  2:502965/140‑1 (MQ=255)

                                                                                           ttcttTCATCATGTGGCTGGAAAGCTCTTCATGCAGCATGGCCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGAACGCGCTCGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGt                                       <  1:55333/140‑1 (MQ=255)

                                                                                           ttcttTCATCATGTGGCCGGAACGCTCTTCAAGCACCATCGCCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGAACGCGCTCGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGt                                       <  2:193923/140‑1 (MQ=255)

                                                                                            tcttTCATCATGTGGCTGGAAAGCTCTTCATGCAGCATGGCCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGAACGCGCTCGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTg                                      <  2:180248/140‑1 (MQ=255)

                                                                                            tcttTCATCATGTCGCAGGAAAGCTCTTCATACGGGATGTCCAGGTATATTGTCAGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGAACGCGCTCGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTg                                      <  2:331218/140‑1 (MQ=255)

                                                                                             cttTCATCATGTGGCTGGAAAGCTCTTCATGCAGCATGGCCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGAACGCGCTCGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTg                                      >  2:409284/1‑139 (MQ=255)

                                                                                             cttTCATCATGTGGCTGGAAAGCTCTTCATGCAGCATGGCCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGAACGCGCTCGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTg                                      <  1:409284/139‑1 (MQ=255)

                                                                                              tttCATCATGTGGCTGTAAAGCCCTTCATGTCGTATGGCTAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGAACGCGCTCGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGAc                                    <  2:347034/140‑1 (MQ=255)

                                                                                                tcatcaTGTGGCTGGAAAGCTCTTCATGCAGCATGGCCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGAACGCGCTCGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGa                                  >  2:444387/1‑140 (MQ=255)

                                                                                                  atcatGTGGCTGGAAAGCTCTTCATGCAGCATGGCCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGAACGCGCTCGACTTTAGTCGCTGGCAGAATGAGCGCCAGCAGTTGCTCGCGGTGACGATc                                >  1:347034/1‑140 (MQ=255)

                                                                                                       gtgGCTGGAAAGCTCTTCATGCAGCATGGCCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGAACGCGCTCGACTTTAGTCGCTTGCGGAACCAGATCCGGCAGTTGCTCGCGGTGACGATCGTGGt                           >  2:304570/1‑140 (MQ=255)

                                                                                                       gtgGCTGGAAAGCTCTTCATGCAGCATGGCCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGAACGCGCTCGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTg                                      <  1:666038/129‑1 (MQ=255)

                                                                                                       gtgGCTGGAAAGCTCTTCATGCAGCATGGCCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGAACGCGCTCGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTg                                      >  2:666038/1‑129 (MQ=255)

                                                                                                       gtgGCTGGAAAGCTCTTCATGCAGCATGGCCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGAACGCGCTCGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGt                           >  2:638961/1‑140 (MQ=255)

                                                                                                       gtgGCTGGAAAGCTCTTCATGCAGCATGGCCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGAACGCGCTCGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGt                           >  2:716375/1‑140 (MQ=255)

                                                                                                        tgGCTGGAAAGCTCTTCATGCAGCATGGCCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGTTTGTCGGCGTCAACGCCCTCGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTa                          >  2:84359/1‑140 (MQ=255)

                                                                                                          gCTGGAAAGCTCTTCATGCAGCATGGCCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGAACGCGCTCGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAgc                        >  2:583636/1‑140 (MQ=255)

                                                                                                          gCTGGAAAGCTCTTCATGCAGCATGGCCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGAACGCGCTCGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAgc                        <  1:638961/140‑1 (MQ=255)

                                                                                                           cTGGAAAGCTCTTCATGCAGCATGGCCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGAACGCGCTCGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAgcg                       <  2:390940/140‑1 (MQ=255)

                                                                                                           cTGGAAAGCTCTTCAAGCAGCAAGGGCAGGTATTTTGGCAGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGAACGCGCTCGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAgcg                       <  1:304570/140‑1 (MQ=255)

                                                                                                             ggAAAGCTCTTCATGCAGCATGGCCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGAACGCGCTCGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCa                     <  1:525604/140‑1 (MQ=255)

                                                                                                              gAAAGCTCTTCATGCAGCATGGCCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGAACGCGCTCGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCAc                    >  1:75489/1‑140 (MQ=255)

                                                                                                               aaaGCTCTTCATGCAGCATGGCCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGAACGCGCTCGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACg                   <  2:104082/140‑1 (MQ=255)

                                                                                                               aaaGCTCTACATGCAGCATGGCAAGGTATTTTGTCGGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGAACGCGCTCGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACg                   <  2:336599/140‑1 (MQ=255)

                                                                                                                    tctTCATGCAGCATGGCCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGAACGCGCTCGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACgatgat              <  1:583636/140‑1 (MQ=255)

                                                                                                                    tctTCATGCAGCATGGCCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGAACGCGCTCGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACgatgat              <  1:105482/140‑1 (MQ=255)

                                                                                                                               caTGGCCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGAACGCGCTCGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACGATGATATGGTCgatga   <  1:492450/140‑1 (MQ=255)

                                                                                                                                aTGGCCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGAACGCGCTCGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACGATGATATGGTCgatgat  >  2:387208/1‑140 (MQ=255)

                                                                                                                                aTGGCCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGAACGCGCTCGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGACCGTGGTAGCGCACCATGATATGGTCgatgat  >  2:296743/1‑140 (MQ=255)

CCGCTTCATCGTGCTCGCTTTCCATTACGCTGATTGGCCCCATTGCCTGGCTGCCCATGCCTTGTTTGATCATCGGGAAGAGGATCTGCTCTTCTTTCATCATGTGGCTGGAAAGCTCTTCATGCAGCATGGTCAGGTATTTTGTCAGCCCTTTTGGCACGCTCGGTTTGTCGGCGTGAACGCGCTCGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACGATGATATGGTCGATGAT  >  NC_000913/4431494‑4431761

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A