Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A46 F60 I1 R1 20 83.7 2881312 98.9% 2849617 138.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,028,818 C→G E718D (GAG→GAC)  fadB ← dodecenoyl‑CoA delta‑isomerase, enoyl‑CoA hydratase, 3‑hydroxybutyryl‑CoA epimerase, 3‑hydroxyacyl‑CoA dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,028,8180CG99.0% 382.1 / NA 103E718D (GAG→GAC) fadBdodecenoyl‑CoA delta‑isomerase, enoyl‑CoA hydratase, 3‑hydroxybutyryl‑CoA epimerase, 3‑hydroxyacyl‑CoA dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base G (37/65);  total (38/65)
Fisher's exact test for biased strand distribution p-value = 3.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 105 total aligned reads displayed.

GAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGCGAATTGCATCGACAATGACAACCTGTTCCATTGTGACTCCTTAAGCCGTTTTCAGGTCGCCAACCGGACGGGCTGGCTCAACCGGAGGATAGTACGGTTCGTTATGACGCGCTTTATTACGCAGACCTTCCGGCACTTCATACAGCGGGCCGAGGTGCTGATATTGCTGTGCCATATCGAGGTATTTTGCGCTACCGAG  >  NC_000913/4028681‑4028940
                                                                                                                                         |                                                                                                                          
gagagaTCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGCGAATTGCATCGACAATGACAACCTGTTCCATTGTGACTCCTTAAGCCGTTTTCAGGTCGCCAACCGGACGGGCTGGGTc                                                                                                                          <  2:1243063/140‑1 (MQ=255)
gagagaTCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGCGAATTGCATCGACAATGACAACCTGTTCCATTGTGACTCCTTAAGCCGTTTTCAGGTCGCCAACCGGACGGGCTGGGTc                                                                                                                          >  2:238403/1‑140 (MQ=255)
gagagaTCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGCGAATTGCATCGACAATGACAACCTGTTCCATTGTGACTCCTAAAGCCGTATTCTGGACGCCAACCGGACGGGCTGGGTc                                                                                                                          >  1:485271/1‑140 (MQ=255)
  gagaTCTTCTGCACGAACGTTACGATAAGCACCGCCCCTCGAACGGCCCATCGGGGTGCGAATTGCATCGACAATGACAACCTGTTCCATTGTGACTCCTTAAGCCGTTTTCAGGTCGCCAACCGGACGGGCTGGGTCaa                                                                                                                        <  1:380933/140‑1 (MQ=255)
  gagaTCTTCTGCACGAACGTTAAGACAAGTACCGCCCTTCGAACGGCCCATCGGGGTGCGAATTGCATCGACAATGACAACCTGTTCCATTGTGACTCCTTAAGCCGTTTTCAGGTCGCCAACCGGACGGGCTGGGTCaa                                                                                                                        <  1:425369/140‑1 (MQ=255)
    gaTCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGCGAATTGCATCGACAATGACAACCTGTTCCATTGTGACTCCTTAAGCCGTTTTCAGGTCGCCAACCGGACGGGCTGGGTCAAcc                                                                                                                      <  2:162767/140‑1 (MQ=255)
     aTCTTCTGTACGCACGACACGACAAGCACCTTCATTCGATCGGCCCATTGGGGTGCGAATTGCATCGACAATGACAACCTGTTCCATTGTGACTCCTTAAGCCGTTTTCAGGTCGCCAACCGGACGGGCTGGGTCAACCg                                                                                                                     <  2:683203/140‑1 (MQ=255)
     aTCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGCGAATTGCATCGACAATGACAACCTGTTCCATTGTGACTCCTTAAGCCGTTTTCAGGTCGCCAACCGGACGGGCTGGGTCAACCg                                                                                                                     <  2:186247/140‑1 (MQ=255)
     aTCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGCGAATTGCATCGACAATGACAACCTGTTCCATTGTGACTCCTTAAGCCGTTTTCAGGTCGCCAACCGGACGGGCTGGGTCAACCg                                                                                                                     <  2:894722/140‑1 (MQ=255)
     aTCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGCGAATTGCATCGACAATGACAACCTGTTCCATTGTGACTCCTTAAGCCGTTTTCAGGTCGCCAACCGGACGGGCTGGGTCAACCg                                                                                                                     <  1:1369441/140‑1 (MQ=255)
     aTCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGCGAATTGCATCGACAATGACAACCTGTTCCATTGTGACTCCTTAAGCCGTTTTCAGGTCGCCAACCGGACGGGCTGGGTCAACCg                                                                                                                     <  2:849513/140‑1 (MQ=255)
             cacgcacgTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGCGAATTGCATCGACAATGACAACCTGTTCCATTGTGACTCCTTACGCCGTTTTGAGGTCGCGAACCGGACAGGCTGGGTCAACCGGAGGATAg                                                                                                             >  2:380933/1‑140 (MQ=255)
             cacgcacgTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGCGAATTGCATCGACAATGACAACCTGTTCCATTGTGACTCCTTAAGCCGTTTTGAGGGCGCGAACCGGACGGGCTGGGTCAACCGGAGGATAg                                                                                                             >  2:425369/1‑140 (MQ=255)
             cacgcacgTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGCGAATTGCATCGACAATGACAACCTGTTCCATTGTGACTCCTTAAGCCGTTTTCAGGTCGCCAACCGGACGGGCTGGGTCAACCGGAGGATAg                                                                                                             >  2:774020/1‑140 (MQ=255)
               cgcacgTTACGAAAAGCACCGCTCTTCGAACAGCCAATCGGGGTGCGATTTGCATCGACAATGACAACCTGTTCCATTGTGACTCCTTAAGCCGTTTTCAGGTCGCCAACCGGACGGGCTGGGTCAACCGGAGGATAGTa                                                                                                           <  2:531550/140‑1 (MQ=255)
               cgcacgTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGCGAATTGCATCGACAATGACAACCTGTTCCATTGTGACTCCTTAAGCCGTTTTCAGGTCGCCAACCGGACGGGCTGGGTCAACCGGAGGATAGTa                                                                                                           >  2:173264/1‑140 (MQ=255)
               cgcacgTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGCGAATTGCATCGACAATGACAACCTGTTCCATTGTGACTCCTTAAGCCGTTTTCAGGTCGCCAACCGGACGGGCTGGGTCAACCGGAGGATAGTa                                                                                                           <  2:850632/140‑1 (MQ=255)
                gcacgTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGCGAATTGCATCGACAATGACAACCTGTTCCATTGTGACTCCTTAAGCCGTTTTCAGGTCGCCAACCGGACGGGCTGGGTCAACCGGAGGATAGTAc                                                                                                          >  1:536749/1‑140 (MQ=255)
                 cacgTTACGAAAAGCACAGCCCATCGAACGGCCCACCGGTGTGCGAATGGCATCGACAATGACAACCTGTTCCATTGTGACTCCTTAAGCCGTTTTCAGGTCGCCAACCGGACGGGCTGGGTCAACCGGAGGATAGTACg                                                                                                         <  2:545448/140‑1 (MQ=255)
                  acgTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGCGAATTGCATCGACAATGACAACCTGTTCCATTGTGACTCCTTAAGCCGTTTTCAGGTCGCCAACCGGACGGGCTGGGTCAACCGGAGGATAGTACgg                                                                                                        >  2:860703/1‑140 (MQ=255)
                   cgTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGCGAATTGCATCGACAATGACAACCTGTTCCATTGTGACTCCTTAAGCCGTTTTCAGGTCGCCAACCGGACGGGCTGGGTCAACCGGAGGATAGTACGGt                                                                                                       <  1:959897/140‑1 (MQ=255)
                    gTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGCGAATTGCATCGACAATGACAACCTGTTCCATTGTGACTCCTTAAGCCGTTTTCAGGTCGCCAACCGGACGGGCTGGGTCAACCgga                                                                                                                   <  2:110244/127‑1 (MQ=255)
                    gTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGCGAATTGCATCGACAATGACAACCTGTTCCATTGTGACTCCTTAAGCCGTTTTCAGGTCGCCAACCGGACGGGCTGGGTCAACCgga                                                                                                                   >  1:110244/1‑127 (MQ=255)
                    gTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGCGAATTGCATCGACAATGACAACCTGTTCCATTGTGACTCCTTAAGCCGTTTTCAGGTCGCCAACCGGACAGGCTGGCTCAACCGGAGGATAGTACGGtt                                                                                                      >  1:531550/1‑140 (MQ=255)
                     ttACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGCGAATTGTATCGACAATGACAACCTGTTCCATTGTGACTCCTTAAGCCGTTTGCAGGTCGCTAACTGGAGGGGCTGGGACAACAGGAGGATAGTACGGTTc                                                                                                     >  2:366288/1‑140 (MQ=255)
                           aaaGCACCGCCCTTCGAACGGCCCATCGGGGTGCGAATTGCATCGACAATGACAACCTGTTCCATTGTGACTCCTTAAGCCGTTTTCAGGTCGCCAACCGGACGGGCTGGGTCAACCGGAGGATAGTACGGTTCGTTATg                                                                                               <  1:173264/140‑1 (MQ=255)
                            aaGCACCGCCCTTCGAACGGCCCATCGGGGTGCGAATTGCATCGACAATGACAACCTGTTCCATTGTGACTCCTTAAGCCGTTTTCAGGTCGCCAACCGGACGGGCTGGGTCAACCGGAGGATAGTACGGTTCGTTATGa                                                                                              >  2:893695/1‑140 (MQ=255)
                             aGCACCGCCCTTCGAACGGCCCATCGGGGTGCGAATTGCATCGACAATGACAACCTGTTCCATTGTGACTCCTTAAGCCGTTTTCAGGTCGCCAACCGGACGGGCTGGGTCAACCGGAGGATAGTACGGTTCGTTATGAc                                                                                             <  1:890961/140‑1 (MQ=255)
                             aGCACCGCCCTTCGAACGGCCCATCGGGGTGCGAATTGCATCGACAATGACAACCTGTTCCATTGTGACTCCTTAAGCCGTTTTCAGGTCGCCAACCGGACGGGCTGGGTCAACCGGAGGATAGTACGGTTCGTTATGAc                                                                                             <  1:1001345/140‑1 (MQ=255)
                              gCACCGCCCTTCGAACGGCCCATCGGGGTGCGAATTGCATCGACAATGACAACCTGTTCCATTGTGACTCCTTAAGCCGTTTTCAGGTCGCCAACCGGACTGGCCGGGCCAACCGGAGGATATTACGGTTCGTTATGAcg                                                                                            >  2:608569/1‑140 (MQ=255)
                              gCACCGCCCTTCGAACGGCCCATCGGGGTGCGAATTGCATCGACAATGACAACCTGTTCCATTGTGACTCCTTAAGCCGTTTTCAGGTCGCCAACCGGACGGGCTGGGTCAACCGGAGGATAGTACGGTTCGTTATGAc                                                                                             >  2:1033134/1‑139 (MQ=255)
                              gCACCGCCCTTCGAACGGCCCATCGGGGTGCGAATTGCATCGACAATGACAACCTGTTCCATTGTGACTCCTTAAGCCGTTTTCAGGTCGCCAACCGGACGGGCTGGGTCAACCGGAGGATAGTACGGTTCGTTATGAc                                                                                             <  1:1033134/139‑1 (MQ=255)
                               cACCGCCCTTCGAACGGCCCATCGGGGTGCGAATTGCATCGACAATGACAACCTGTTCCATTGTGACTCCTTAAGCCGTTTTCAGGTCGCCAACCGGACGGGCTGGGTCAACCGGAGGATAGTACGGTTCGTTATGAcgc                                                                                           >  1:1117311/1‑140 (MQ=255)
                                aCCGCCCTTCGAACGGCCCATCGGGGTGCGAATTGCATCGACAATGACAACCTGTTCCATTGTGACTCCTTAAGCCGTTTTCAGGTCGCCAACCGGACGGGCTGGGTCAACCGGAGGATAGTACGGTTCGTTATGAcgcg                                                                                          <  2:249131/140‑1 (MQ=255)
                                aCCGCCCTTCGAACGCCCCATCCGGCTGCTAATTGTATCGACAATGACAACCTGTTCCATTGTGACTCCTTAAGCCGTTTTCAGGTCGCCAACCGGACGGGCTGGGTCAACCGGAGGATAGTACGGTTCGTTATGAcgcg                                                                                          <  1:366288/140‑1 (MQ=255)
                                   gCCCTTCGAACGGCCCATCGGGGTGCGAATTGCATCGACAATGACAACCTGTTCCATTGTGACTCCTTAAGCCGTTTTCAGGTCGCCAACCGGACGGGCTGGGTCAACCGGAGGATAGTACGGTTCGTTATGACGCGCtt                                                                                       >  1:903660/1‑140 (MQ=255)
                                    cccTTCGAACGGCCCATCGGGGTGCGAATTGCATCGACAATGACAACCTGTTCCATTGTGACTCCTTAAGCCGTTTTCAGGTCGCCAACCGGACGGGCTGGGTCAACCGGAGGATAGTATGGTTCGTTATGACGCGCttt                                                                                      >  2:1279024/1‑140 (MQ=255)
                                    cccTTCGAACGGCCCATCGGGGTGCGAATTGCATCGACAATGACAACCTGTTCCATTGTGACTCCTTAAGCCGTTTTCAGGTCGCCAACCGGACGGGCTGGGTCAACCGGAGGATAGTACGGTTCGTTATGACGCGCttt                                                                                      >  1:739054/1‑140 (MQ=255)
                                    cccTTCGAACGGCCCATCGGGGTGCGAATTGCATCGACAATGACAACCTGTTCCATTGTGACTCCTTAAGCCGTTTTCAGGTCGCCAACCGGACGGGCTGGGTCAACCGGAGGATAGTACGGTTCGTTATGACGCGCttt                                                                                      >  2:666/1‑140 (MQ=255)
                                     ccTTCGAACGGCCCATCGGGGTGCGAATTGCATCGACAATGACAACCTGTTCCATTGTGACTCCTTAAGCCGTTTTCAGGTCGCCAACCGGACGGGCTGGGTCAACCGGAGGATAGTACGGTTCGTTATGACGCGCTtta                                                                                     <  2:739054/140‑1 (MQ=255)
                                         cGAACGGCCCATCGGGGTGCGAATTGCATCGACAATGACAACCTGTTCCATTGTGACTCCTTAAGCCGTTTTCAGGTCGCCAACCGGACGGGCTGGGTCAACCGGAGGATAGTACGGTTCGTTATGACGCGCTTTATTAc                                                                                 >  1:1185934/1‑140 (MQ=255)
                                            aCGGCCCATCGGGGTGTGAATTACATCGACAATGACAACCTGTTCCATGGTGACTCCTTAAGCCGTTTTCAGGTCGCCAACCGGACGGGCTGGGTCAACCGGAGGATAGTACGGTTCGTTATGACGCGCTTTATTACGCa                                                                              <  2:623554/140‑1 (MQ=255)
                                            aCGGCCCATCGGGGTGCGAATTGCATCGACAATGACAACCTGTTCCATTGTGACTCCTTAAGCCGTTTTCAGGTCGCCAACCGGACGGGCTGGGTCAACCGGAGGATAGTACGGTTCGTTATGACGCGCTTTATTACGCa                                                                              <  2:934502/140‑1 (MQ=255)
                                              ggCCCATCGGGGTGCGAATTGCATCGACAATGACAACCTGTTCCATTGTGACTCCTTAAGCCGTTTTCAGGTCGCCAACCGGACGGGCTGGGTCAACCGGAGGATAGTACGGTTCGTTATGACGCGCTTTATTACGCAGa                                                                            <  2:1092863/140‑1 (MQ=255)
                                                   aTCGGGGTGCGAATTGCATCGACAATGACAACCTGTTCCATTGTGACTCCTTAAGCCGTTTTCAGGTCGCCAACCGGACGGGCTGGGTCAACCGGAGGATAGTACGGTTCGTTATGACGCGCTTTATTACGCAGACCTTc                                                                       <  2:728783/140‑1 (MQ=255)
                                                     cGGGGTGCGAATTGCCTCGACAATGACAACCTGTTCCCTTGTGACTCCTTAAGCCGTTTTCAGGTCGCCAACCGGACGGGCTGGGTCAACCGGAGGATAGTACGGTTCGTTATGACGCGCTTTATTACGCAGACCTTCCg                                                                     <  1:471191/140‑1 (MQ=255)
                                                     cGGGGTGCGAATTGCATGGACAATGACAACCAGTTTCATGGTGACTCCGTAAGCCGTTTTCAGGTCGCCAACCGGACGGGCTGGGTCAACCGGAGGATAGTACGGTTCGTTATGACGCGCTTTATTACGCAGACCTTCCg                                                                     <  2:485271/140‑1 (MQ=255)
                                                     cGGGGTGCGAATTGCATCGACAATGACAACCTGTTCCATTGTGACTCCTTAAGCCGTTTTCAGGTCGCCAACCGGACGGGCTGGGTCAACCGGAGGATAGTATGGTTCGTTATGACGCGCTTTATTACGCAGACCTTCCg                                                                     <  1:1279024/140‑1 (MQ=255)
                                                     cGGGGTGCGAATTGCATCGACAATGACAACCTGTTCCATTGTGACTCCTTAAGCCGTTTTCAGGTCGCCAACCGGACGGGCTGGGTCAACCGGAGGATAGTACGGTTCGTTATGACGCGCTTTATTACGCAGACCTTCCg                                                                     <  1:860703/140‑1 (MQ=255)
                                                     cGGGGTGCGAATTGCATCGACAATGACAACCTGTTCCATTGTGACTCCTTAAGCCGTTTTCAGGTCGCCAACCGGACGGGCTGGGTCAACCGGAGGATAGTACGGTTCGTTATGACGCGCTTTATTACGCAGACCTTCCg                                                                     >  2:916935/1‑140 (MQ=255)
                                                     cGGGGTGCGAATTGCATCGACAATGACAACCTGTTCCATTGTGACTCCTTAAGCCGTTTTCAGGTCGCCAACCGGACGGGCTGGGTCAACCGGAGGATAGTACGGTTCGTTATGACGCGCTTTATTACGCAGACCTTCCg                                                                     <  2:1185934/140‑1 (MQ=255)
                                                     cGGGGTGCGAATTGCATCGACAATGACAACCTGTTCCATTGTGACTCCTTAAGCCGTTTTCAGGTCGCCAACCGGACGGGCTGGGTCAACCGGAGGATAGTACGGTTCGTTATGACGCGCTTTATTACGCAGACCTTCCg                                                                     <  1:238403/140‑1 (MQ=255)
                                                       gggTGCGAATTGCATCGACAATGACAACCTGTTCCATTGTGACTCCTTAAGCCGTTTTCAGGTCGCCAACCGGACGGGCTGGGTCAACCGGAGGATAGTACGGTTCGTTATGACGCGCTTTATTACGCAGACCTTCCGGc                                                                   <  1:893695/140‑1 (MQ=255)
                                                        ggTGCGAATTGCATCGACAATGACAACCTGTTCCATTGTGACTCCTTAAGCCGTTTTCAGGTCGCCAACCGGACGGGCTGGGTCAACCGGAGGATAGTACGGTTCGTTATGACGCGCTTTATTACGCAGACCTTCCGGCa                                                                  <  2:1114907/140‑1 (MQ=255)
                                                             gAATTGCATCGACAATGACAACCTGTTCCATTGTGACTCCTTAAGCCGTTTTCAGGTCGCCAACCGGACGGGCTGGGTCAACCGGAGGATAGTACGGTTCGTTATGACGCGCTTTATTACGCAGACCTTCCGGCACTTCa                                                             <  1:1022524/140‑1 (MQ=255)
                                                              aaTTGCATCGACAATGACAACCTGTTCCATTGTGACTCCTTAAGCCGTTTTCAGGTCGCCAACCGGACGGGCTGGGTCAACCGGAGGATAGTACGGTTCGTTATGACGCGCTTTATTACGCAGACCTTCCGGCACTTAAt                                                            >  1:198949/1‑140 (MQ=255)
                                                                 tGCATCGACAATGACTACCTGTTCCATTGCGACTCCTCAAGCCGTTTTCAGGTCGCCAACCGGACGGGCTGGGTCAACCGGAGGATAGTACGGTTCGTTATGACGCGCTTTATTACGCAGACCTTCCGGCACTTCATACa                                                         <  1:693047/140‑1 (MQ=255)
                                                                 tGCATCGACAATGACAACCTGTTCCATTGTGACTCCTTAAGCCGTTTTCAGGTCGCCAACCGGACGGGCTGGGTCAACCGGAGGATAGTACGGTTCGTTATGACGCGCTTTATTACGCAGACCTTCCg                                                                     >  1:5427/1‑128 (MQ=255)
                                                                 tGCATCGACAATGACAACCTGTTCCATTGTGACTCCTTAAGCCGTTTTCAGGTCGCCAACCGGACGGGCTGGGTCAACCGGAGGATAGTACGGTTCGTTATGACGCGCTTTATTACGCAGACCTTCCg                                                                     <  2:5427/128‑1 (MQ=255)
                                                                   cATCGACAATGACAACCTGTTCCATTGTGACTCCTTAAGCCGTTTTCGGGTCGCCAACCGGACGGGCTGGGTCAACCGGAGGATAGTACGGTTCGTTATGACGCGCTTTATTACGCAGACCTTCCGGCACTTCATACAGc                                                       >  2:1112433/1‑140 (MQ=255)
                                                                    aTCGACAATGACAACCTGTTCCATTGTGACTCCTTAAGCCGTTTTCAGGTCGCCAACCGGACGGGCTGGGTCAACCGGAGGATAGTACGGTTCGTTATGACGCGCTTTATTACGCAGACCTTCCGGCACTTCATACAGCg                                                      <  2:1210326/140‑1 (MQ=255)
                                                                        aCAATGACAACCTGTTCCATTGTGACTCCTTAAGCCGTTTTCAGGTCGCCAACCGGACGGGCTGGGTCAACCGGAGGATAGTACGGTTCGTTATGACGCGCTTTATTACGCAGACCTTCCGGCACTTCATACAGCGGGcc                                                  <  2:973338/140‑1 (MQ=255)
                                                                        aCAATGACAACCTGTTCCATTGTGACTCCTTAAGCCGTTTTCAGGTCGCCAACCGGACGGGCTGGGTCAACCGGAGGATAGTACGGTTCGTTATGACGCGCTTTATTACGCAGACCTTCCGGCACTTCATACAGCGGGcc                                                  >  2:927411/1‑140 (MQ=255)
                                                                         cAATGACAACCTGTTCCATTGTGACTCCTTAAGCCGTTTCCAGGTCGCCAACCGGACGGGCTGGGTCAACCGGAGGATAGTACGGTTCGTTATGACGCGCTTTATTACGCAGACCTTCCGGCACTTCATACAGCGGGCCg                                                 <  2:536749/140‑1 (MQ=255)
                                                                             gACAACCTGTTCCATTGTGACTCCTTAAGCCGTTTTCGGGTCGCCAACCGGACGGGCTGGGTCAACCGGAGGATAGTACGGTTCGTTATGACGCGCTTTATTACGCAGACCTTCCGGCACTTCATACAGCGGGCCGAGGt                                             <  1:1112433/140‑1 (MQ=255)
                                                                             gACAACCTGTTCCATTGTGACTCCTTAAGCCGTTTTCAGGTCGCCAACCGGACGGGCTGGGTCAACCGGAGGATAGTACGGTTCGTTATGACGCGCTTTATTCCGCAGACATTCGGGGACTTCAGACAGCGGGCCGAGGt                                             >  1:697424/1‑140 (MQ=255)
                                                                             gACAACCTGTTCCATTGTGACTCCTTAAGCCGTTTTCAGGTCGCCAACCGGACGGGCTGGGTCAACCGGAGGATAGTACGGTTCGTTATGACGCGCTTTATTACGCAGACCTTCCGGCACTTCATACAGTGGGCCGAGGt                                             <  2:1158259/140‑1 (MQ=255)
                                                                                  ccTGTTCCATTGTGACTCCTTAAGCCGTTTTCAGGTCGCCAACCGGACGGGCTGGGTCAACCGGAGGATAGTACGGTTCGTTATGACGCGGGTTATTACGCAGAGCTTGCGGCACTTAATACCGCGGGCCGAGGTGCTGa                                        >  2:449040/1‑140 (MQ=255)
                                                                                   cTGTTCCATTGTGACTCCTTAAGCCGTTTTCAGGTCGCCAACCGGACGGGCTGGGTCAACCGGAGGATAGTACGGTTCGTTATGACGCGCTTTATTACGCAGACCTTCCGGCACTTCATACAGCGGGCCGAGGTGCTGat                                       >  1:787072/1‑140 (MQ=255)
                                                                                      ttCCATTGTGACTCCTTAAGCCGTTTTCAGGTCGCCAACCGGACGGGCTGGGTCAACCGGAGGATAGTACGGTTCGTTATGACGCGCTTTATTACGCAGACCTTCCGGCACTTCATACAGCGGGCCGAGGTGCTGATAtt                                    >  2:1279279/1‑140 (MQ=255)
                                                                                       tCCATTGTGACTCCTTAAGCCGTTTTCAGGTCGCCAACCGGACGGGCTGGGTCAACCGGAGGATAGTACGGTTCGTTATGACGCGCTTTATTACGCAGACCTTCCGGCACTTCATACAGCGGGCCGAGGt                                             >  2:1436629/1‑130 (MQ=255)
                                                                                       tCCATTGTGACTCCTTAAGCCGTTTTCAGGTCGCCAACCGGACGGGCTGGGTCAACCGGAGGATAGTACGGTTCGTTATGACGCGCTTTATTACGCAGACCTTCCGGCACTTCATACAGCGGGCCGAGGt                                             <  1:1436629/130‑1 (MQ=255)
                                                                                       tCCATTGTGACTCCTTAAGCCGTTTTCAGGTCGCCAACCGGACGGGCTGGGTCAACCGGAGGATAGTACGGTTCGTTATGACGCGCTTTATTACGCAGACCTTCCGGCACTTCATACAGCGGGCCGAGGTGCTGATATTg                                   <  2:825454/140‑1 (MQ=255)
                                                                                         cATTGTGACTCCTTAAGCCGTTTTCAGGTCGCCAACCGGACGGGCTGGGTCAACCGGAGGATAGTACGGTTCGTTATGACGCGCTTTATTACGCAGACCTTCCGGCACTTCATACAGCGGGCCGAGGTGCTGATATTGCt                                 <  1:527329/140‑1 (MQ=255)
                                                                                         cATTGTGACTCCTTAAGCCGTTTTCAGGTCGCCAACCGGACGGGCTGGGTCAACCGGAGGATAGTACGGTTCGTTATGACGCGCTTTATTACGCAGACCTTCCGGCACTTCATACAGCGGGCCGAGGTGCTGATATTGCt                                 <  1:785586/140‑1 (MQ=255)
                                                                                            tgtgACTCCTTAAGCCGTTTTCAGGTCGCCAACCGGACGGGCTGGGTCAACCGGAGGATAGTACGGTTCGTTATGACGCGCTTTATTACGCAGACCTTCCGGCACTTCATACAGCGGGCCGa                                                >  2:198371/1‑122 (MQ=255)
                                                                                            tgtgACTCCTTAAGCCGTTTTCAGGTCGCCAACCGGACGGGCTGGGTCAACCGGAGGATAGTACGGTTCGTTATGACGCGCTTTATTACGCAGACCTTCCGGCACTTCATACAGCGGGCCGa                                                <  1:198371/122‑1 (MQ=255)
                                                                                            tgtgACTCCTTAAGCCGTTTTCAGGTCGCCAACCGGACGGGCTGGGTCAACCGGAGGATAGTACGGTTCGTTATGACGCGCTTTATTACGCAGACCTTCCGGCACTTCATACAGCGGGCCGAGGTGCTGATATTGCtgtg                              >  1:1339767/1‑140 (MQ=255)
                                                                                            tgtgACTCCTTAAGCCGTTTTCAGGTCGCCAACCGGACGGGCTGGGTCAACCGGAGGATAGTACGGTTCGTTATGACGCGCTTTATTACGCAGACCTTCCGGCACTTCATACAGCGGGCCGAGGTGCTGATATTGCtgtg                              <  2:1334878/140‑1 (MQ=255)
                                                                                              tgACTCCTTAAGCCGTTTTCAGGTCGCCAACCGGACGGGCTGGGTCAACCGGAGGATAGTACGGTTCGTTATGACGCGCTTTATTACGCAGACCTTCCGGCACTTCATACAGCGGGCCGAGGTGCTGATATTGCtgtg                              >  1:945221/1‑138 (MQ=255)
                                                                                              tgACTCCTTAAGCCGTTTTCAGGTCGCCAACCGGACGGGCTGGGTCAACCGGAGGATAGTACGGTTCGTTATGACGCGCTTTATTACGCAGACCTTCCGGCACTTCATACAGCGGGCCGAGGTGCTGATATTGCtgtg                              <  2:945221/138‑1 (MQ=255)
                                                                                                aCTCCTTAAGCCATTTTCAGGTCGCCAACCGGACGGGCTGGGTCAACCGGAGGATAGTACGGTTCGTTATGACGCGCTTTATTACGCAGACCTTCCGGCACTTCATACAGCGGGCCGAGGTGCTGATATTGCTGTGCCat                          >  2:973055/1‑140 (MQ=255)
                                                                                                   ccTTAAGCCGTTTTCAGGTCGCCAACCGGACGGGCTGGGTCAACCGGAGGATAGTACGGTTCGTTATGACGCGCTTTATTACGCAGACCTTCCGGCACTTCATACAGCGGGCCGAGGTGCTGATATTGCTGTGCCATATc                       >  1:1149579/1‑140 (MQ=255)
                                                                                                   ccTTAAGCCATTTTCAGGTCGCCAACCGGACGGGCTGGGTCAACCGGAGGATAGTACGGTTCGTTATGACGCGCTTTATTACGCAGACCTTCCGGCACTTCATACAGCGGGCCGAGGTGCTGATATTGCTGTGCCATATc                       <  1:973055/140‑1 (MQ=255)
                                                                                                     ttAAGCCGTTTTCAGGGTGCCAGCCGGACGGGCTGCGTCAACCGGAGGGTAGTACGGTTCGTTATGACGCGCTTTATTACGCAGACCTTCCGGCACTTCATACAGCGGGCCGAGGTGCTGATATTGCTGTGCCATATCGa                     <  2:617681/140‑1 (MQ=255)
                                                                                                      tAAGCCGTTTTCAGGTCGCCAACCGGACGGGCTGGGTCAACCGGAGGATAGTACGGTTCGTTATGACGCGCTTTATTACGCAGACCTTCCGGCACTTCATACAGCGGGCCGAGGTGCTGATATTGCTGTGCCata                         <  2:1205815/135‑1 (MQ=255)
                                                                                                      tAAGCCGTTTTCAGGTCGCCAACCGGACGGGCTGGGTCAACCGGAGGATAGTACGGTTCGTTATGACGCGCTTTATTACGCAGACCTTCCGGCACTTCATACAGCGGGCCGAGGTGCTGATATTGCTGTGCCata                         >  1:1205815/1‑135 (MQ=255)
                                                                                                       aaGGCGTTTTCAGGTCGCCAACCGGACGGGCTGGGTCAACCGGAGGATAGTACGGTTCGTTATGACGCGCTTTATTACGCAGACCTTCCGGCACTTCATACAGCGGGCCGAGGTGCTGATATTGCTGTGCCATATCGAgg                   <  2:198949/140‑1 (MQ=255)
                                                                                                              tttCAGGTCGCCAACCGGACGGGCTGGGTCAACCGGAGGATAGTACGGTTCGTTATGACGCGCTTTATTACGCAGACCTTCCGGCACTTCATACAGCGGGCCGAGGTGCTGATATTGCTGTGCCATATCGAGGTATTTTg            >  2:106979/1‑140 (MQ=255)
                                                                                                               ttCAGGTCGCCAACCGGACGGGCTGGGTCAACCGGAGGATAGTACGGTTCGTTATGACGCGCTTTATTACGCAGACCTTCCGGCACTTCATACAGCGGGCCGAGGTGCTGATATTGCTGTGCCATATCGAGGTATTTTgc           <  1:199080/140‑1 (MQ=255)
                                                                                                               ttCAGGTCGCCAACCGGACGGGCTGGGTCAACCGGAGGATAGTACGGTTCGTTATGACGCGCTTTATTACGCAGACCTTCCGGCACTTCATACAGCGGGCCGAGGTGCTGATATTGCTGTGCCATATCGAGGTATTTTgc           <  2:903660/140‑1 (MQ=255)
                                                                                                                tCAGGTCGCCAACCGGACGGGCTGGGTCAACCGGAGGATAGTACGGTTCGTTATGACGCGCTTTATTACGCAGACCTTCCGGCACTTCATACAGCGGGCCGAGGTGCTGATATTGCTGTGCCATATCGAGGTATTTTgcg          <  2:1117311/140‑1 (MQ=255)
                                                                                                                tCAGGTCGCCAACCGGACGGGCTGGGTCAACCGGAGGATAGTACGGTTCGTTATGACGCGCTTTATTACGCAGACCTTCCGGCACTTCATACAGCGGGCCGAGGTGCTGATATTGCTGTGCCATATCGAGGTATTTTgcg          <  2:874559/140‑1 (MQ=255)
                                                                                                                   ggTCGCCACCCGGACGACCTGGGTCAACCGAGGGATAGTACGGTTCGTTATGACGCGCTTTATTACGCAGACCTTCCGGCACTTCATACAGCGGGCCGAGGTGCTGATATTGCTGTGCCATATCGAGGTATTTTGCGCTa       <  2:697424/140‑1 (MQ=255)
                                                                                                                      cGCCAACCGGACGGGATGGGTCTACAGGACGATAGTAGGGTTCGTTATGACGCGGTTTATTACGCAGACCTTCCGGCACTTCATACAGCGGGCCGAGGTGCTGATATTGCTGTGCCATATCGAGGTATTTTGCGCTACCg    <  1:449040/140‑1 (MQ=255)
                                                                                                                       gCCAACCGGACGGGCTGGGTCAACCGGAGGATAGTACGGTTCGTTATGACGCGCTTTATTACGCAGACCTTCCGGCACTTCATACAGCGGGCCGAGGTGCTGATATTGCTGTGCCATATCGAGGTATTTTGCGCTACCGa   <  1:916935/140‑1 (MQ=255)
                                                                                                                       gCCAACCGGACGGGCTGGGTCAACCGGAGGATAGTACGGTTCGTTATGACGCGCTTTATTACGCAGACCTTCCGGCACTTCATACAGCGGGCCGAGGTGCTGATATTGCTGTGCCATATCGAGGTATTTTGCGCTACCGa   >  1:1027497/1‑140 (MQ=255)
                                                                                                                        ccAACCGGACGGGCTGGGTCAACCGGAGGATAGTACGGTTCGTTATGACGCGCTTTATTACGCAGACCTTCCGGCACTTCATACAGCGGGCCGAGGTGCTGATATTGCTGTGCCATATCGAGGTATTTTGCGCTACCGAg  <  2:1339767/140‑1 (MQ=255)
                                                                                                                        ccAACCGGACGGGCTGGGTCAACCGGAGGATAGTACGGTTCGTTATGACGCGCTTTATTACGCAGACCTTCCGGCACTTCATACAGCGGGCCGAGGTGCTGATATTGCTGTGCCATATCGAGGTATTTTGCGCTACCGAg  <  1:1321084/140‑1 (MQ=255)
                                                                                                                        ccAACCGGACGGGCTGGGTCAACCGGAGGATAGTACGGTTCGTTATGACGCGCTTTATTACGCAGACCTTCCGGCACTTCATACAGCGGGCCGAGGTGCTGATATTGCTGTGCCATATCGAGGTATTTTGCGCTACCGAg  <  2:1027497/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                          
GAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGCGAATTGCATCGACAATGACAACCTGTTCCATTGTGACTCCTTAAGCCGTTTTCAGGTCGCCAACCGGACGGGCTGGCTCAACCGGAGGATAGTACGGTTCGTTATGACGCGCTTTATTACGCAGACCTTCCGGCACTTCATACAGCGGGCCGAGGTGCTGATATTGCTGTGCCATATCGAGGTATTTTGCGCTACCGAG  >  NC_000913/4028681‑4028940

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A