Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A17 F48 I1 R1
|
90 |
35.3 |
1199032 |
98.5% |
1181046 |
139.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
790,725 |
G→T |
A102E (GCG→GAG) |
galT ← |
galactose‑1‑phosphate uridylyltransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 790,725 | 0 | G | T | 100.0%
| 77.1
/ NA
| 23 | A102E (GCG→GAG) | galT | galactose‑1‑phosphate uridylyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (17/6); total (17/6) |
CATGGGTACGTTTTCCCCAGTTCTGCGGTTTGCTCCTGCCAGGTTTTGACGATTTCCGTCAATGCTGCAACGCTGAGCTCTGGCAGCGTTTTACTGTGATCCGGTGAAAAGCAGATCACCCGGCTGGTGCCGCGCGCGCTCTGGCAACGCATCAGCGGATCGTGACTTTCTGGCGCATCTGGCGTGTCAGACATCAAAGCCGCAAAGTCATTAGTGAAAACGTAAGTCCCGGTGTAATCGGGGTTTTTATCGCCTGTCACCCGC > NC_000913/790590‑790853
|
aaTGGGTACGTTTTCCCCAGTTCTGCGGTTTGCTCCTGCCAGGTTTTGACGATTTCCGTCAATGCTGCAACGCTGAGCTCTGGCAGCGTTTTACTGTGATCCGGTGAAAAGCAGATCACCCGGCTGGTGCCGcgctcgct < 2:19546/139‑1 (MQ=255)
tttCCCCAGTTCTGCGGTTTGCTCCTGCCAGGTTTTGACGATTTCCGTCAATGCTGCAACGCTGAGCTCTGGCAGCGTTTTACTGTGATCCGGTGAAAAGCAGATCACCCGGCTGGTGCCGCGCTCGCTCTGGCAACGCa > 1:295213/1‑140 (MQ=255)
gTTCTGCGGTTTGCTCCTGCCAGGTTTTGACGATTTCCGTCAATGCTGCAACGCTGAGCTCTGGCAGCGTTTTACTGTGATCCGGTGAAAAGCAGATCACCCGGCTGGTGCCGCGCTCGCTCTGGCAACGCATCAGCGGa > 2:406556/1‑140 (MQ=255)
ttCTGCGGCTTTCACCTGCCAGGTTTTGACGATTTCCGTCAATGCTGCAACGCTGAGCTCTGGCAGCGTTTTACTGTGATCCGGTGATAAGCAGATCACCCGGCTGGTGCCGCGCTCGCTCTGGCAACGCATCGGCGGAt > 2:56718/1‑140 (MQ=255)
cGGTTTGCTCCTGCCAGGTTTTGACGATTTCCGTCAATGCTGCAACGCTGAGCTCTGGCAGCGTTTTACTGTGATCCGGTGAAAAGCAGATCACCCGGCTGGTGCCGCGCTCGCTCTGGCAACGCATCAGCGGATCGTGa > 2:47446/1‑140 (MQ=255)
ggTTTGCTCCTGCCAGGTTTTGACGATTTCCGTCAATGCTGCAACGCTGAGCTCTGGCAGCGTTTTACTGTGATCCGGTGAAAAGCAGATCACCCGGCTGGTGCCGCGCTCGCTCTGGCAACGCATCAGCGGATCGTGAc > 1:310387/1‑140 (MQ=255)
gCCAGGTTTTGACGATTTCCGTCAATGCTGCAACGCTGAGCTCTGGCAGCGTTTTACTGTGATCCGGTGAAAAGCAGATCACCCGGCTGGTGCCGCGCTCGCTCTGGCAACGCATCAGCGGATCGTGACTTTCTGGCGCa > 2:57953/1‑140 (MQ=255)
cAGGTTTTGACGATTTCCGTCAATGCTGCAACGCTGAGCTCTGGCAGCGTTTTACTGTGATCCGGTGAAAAGCAGATCACCCGGCTGGTGCCGCGCTCGCTCTGGCAACGCATCAGCGGATCGTGACTTTCTGGCGCATc < 2:295213/140‑1 (MQ=255)
tttCCGTCAATGCTGCAACGCTGAGCTCTGGCAGCGTTTTACTGTGATCCGGTGAAAAGCAGATCACCCGGCTGGTGCCGCGCTCGCTCTGGCAACGCATCAGCGGATCGTGACTTTCTGGCGCATCTGGCGTGTCAGAc > 1:25392/1‑140 (MQ=255)
gctgcAACGCTGAGCTCTGGCAGCGTTTTACTGTGATCCGGTGAAAAGCAGATCACCCGGCTGGTGCCGCGCTCGCTCTGGAAACGCATCAGCGGATCGTGACTTTCTGGCGCATCTGACGTGTCAGACATCAAAGCCGc < 1:56718/140‑1 (MQ=255)
gCTGAGCTCTGGCAGCGTTTTACTGTGATCCGGTGAAAAGCAGATCACCCGGCTGGTGCCGCGCTCGCTCTGGCAACGCATCAGCGGATCGTGACTTTCTGGCGCATCTGGCGTGTCAGACATCAAAGCCGCAAAGTCAt < 2:413688/140‑1 (MQ=255)
tGAGCTCTGGCAGCGTTTTACTGTGATCCGGTGAAAAGCAGATCACCCGGCTGGTGCCGCGCTCGCTCTGGCAACGCATCAGCGGATCGTGACTTTCTGGCGCATCTGGCGTGTCAGACATCAAAGCCGCAAAGTCATTa > 2:555595/1‑140 (MQ=255)
gAGCTCTGGCAGCGTTTTACTGTGATCCGGTGAAAAGCAGATCACCCGGCTGGTGCCGCGCTCGCTCTGGCAACGCATCAGCGGATCGTGACTTTCTGGCGCATCTGGCGTGTCAGACATCAAAGCCGCAAAGTCATTAg > 1:567641/1‑140 (MQ=255)
aGCTCTGGCAGCGTTTTACTGTGATCCGGTGAAAAGCAGATCACCCGGCTGGTGCCGCGCTCGCTCTGGCAACGCATCAGCGGATCGTGACTTTCTGGCGCATCTGGCGTGTCAGACATCAAAGCCGCAAAGTCATTAGt > 2:179372/1‑140 (MQ=255)
gCAGCGTTTTACTGTGATCCGGTGAAAAGCAGATCACCCGGCTGGTGCCGCGCTCGCTCTGGCAACGCATCAGCGGATCGTGACTTTCTGGCGCATCTGGCGTGTCAGACATCAAAGCCGCAAAGTCATTAGTGAAAACg < 2:310387/140‑1 (MQ=255)
tgATCCGGTGAAAAGCAGATCACCCGGCTGGTGCCGCGCTCGCTCTGGCAACGCATCAGCGGATCGTGACTTTCTGGCGCATCTGGCGTGTCAGACATCAAAGCCGCAAAGTCATTAGTGAAAACGTAAGTCCCGGTGTa > 2:167984/1‑140 (MQ=255)
aTCCGGTGAAAAGCAGATCACCCGGCTGGTGCCGCGCTCGCTCTGGCAACGCATCAGCGGATCGTGACTTTCTGGCGCATCTGGCGTGTCAGACATCAAAGCCGCAAAGTCATTAGTGAAAACGTAAGTCCCGGTGTAAt > 1:131055/1‑140 (MQ=255)
tCCGGTGAAAAGCAGATCACCCGGCTGGTGCCGCGCTCGCTCTGGCAACGCATCAGCGGATCGTGACTTTCTGGCGCATCTGGCGTGTCAGACATCAAAGCCGCAAAGTCATTAGTGAAAACGTAAGTCCCGGTGTAATc < 2:25392/140‑1 (MQ=255)
tCCGGTGAAAAGCAGATCACCCGGCTGGTGCCGCGCTCGCTCTGGCAACGCATCAGCGGATCGTGACTTTCTGGCGCATCTGGCGTGTCAGACATCAAAGCCGCAAAGTCATTAGTGAAAACGTAAGTCCCGGTGTAATc > 1:169539/1‑140 (MQ=255)
gTGAAAAGCAGATCACCCGGCTGGTGCCGCGCTCGCTCTGGCAACGCATCAGCGGATCGTGACTTTCTGGCGCATCTGGCGTGTCAGACATCAAAGCCGCAAAGTCATTAGTGAAAACGTAAGTCCCGGTGTAATCgggg > 2:117921/1‑140 (MQ=255)
gaccACCCGGCTGGTGCCGCGCTCGCTCGGGCAACGCATCAGCGGATCGTGTCTTCCTGCCGCATCTGGGGTGTCGGACATAAAAGCCGCAAAGTCATTAGTAAAACCGTAAGTCCCGGTGAAAACGGGGTTTTTATCGc < 2:415306/137‑1 (MQ=255)
ccGGCTGGTGCCGCGCTCGCTCTGGCAACGCATCAGCGGATCGTGACTTTCTGGCGCATCTGGCGTGTCAGACATCAAAGCCGCAAAGTCATTAGTGAAAACGTAAGTCCCGGTGTAATCGGGGTTTTTATCGCCTGTCa > 2:453000/1‑140 (MQ=255)
cGGCTGGTGCCGCGCTCGCTCTGGCAACGCATCAGCGGATCGTGACTTTCTGGCGCATCTGGCGTGTCAGACATCAAAGCCGCAAAGTCATTAGTGAAAACGTAAGTCCCGGTGTAATCGGGGTTTTTATCGCCTGTCAc < 2:567641/140‑1 (MQ=255)
ggCTGGTGCCGCGCTCGCTCTGGCAACGCATCAGCGGATCGTGACTTTCTGGCGCATCTGGCGTGTCAGACATCAAAGCCGCAAAGTCATTAGTGAAAACGTAAGTCCCGGTGTAATCGGGGTTTTTATCGCCTGTCAcc > 2:106082/1‑140 (MQ=255)
gCTGGTGCCGCGCTCGCTCTGGCAACGCATCAGCGGATCGTGACTTTCTGGCGCATCTGGCGTGTCAGACATCAAAGCCGCAAAGTCATTAGTGAAAACGTAAGTCCCGGTGTAATCGGGGTTTTTATCGCCTGTCAccc > 1:339118/1‑140 (MQ=255)
tGGTGCCGCGCTCGCTCTGGCAACGCATCAGCGGATCGTGACTTTCTGGCGCATCTGGCGTGTCAGACATCAAAGCCGCAAAGTCATTAGTGAAAACGTAAGTCCCGGTGTAATCGGGGTTTTTATCGCCTGTCACCCGc > 2:44934/1‑140 (MQ=255)
|
CATGGGTACGTTTTCCCCAGTTCTGCGGTTTGCTCCTGCCAGGTTTTGACGATTTCCGTCAATGCTGCAACGCTGAGCTCTGGCAGCGTTTTACTGTGATCCGGTGAAAAGCAGATCACCCGGCTGGTGCCGCGCGCGCTCTGGCAACGCATCAGCGGATCGTGACTTTCTGGCGCATCTGGCGTGTCAGACATCAAAGCCGCAAAGTCATTAGTGAAAACGTAAGTCCCGGTGTAATCGGGGTTTTTATCGCCTGTCACCCGC > NC_000913/790590‑790853
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
