Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A15 F48 I1 R1 115 87.5 3003888 99.1% 2976853 139.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 179,045 G→T Q37K (CAA→AAA)  mtn ← 5'‑methylthioadenosine/S‑adenosylhomocysteine nucleosidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913179,0450GT100.0% 112.2 / NA 33Q37K (CAA→AAA) mtn5'‑methylthioadenosine/S‑adenosylhomocysteine nucleosidase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (31/2);  total (31/2)

GGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGTTCCAACAGCAAAGTGGCACCCAGCGCCGCAGCGACTTTACCGATGCCCGATTTCAGAAGCGCAACCTCGGTTCCATTCAGTTGGCCGGTATAGATTTCGCAACCGCCGAGACTGATAGTTTGACGGTTTTCGATTTTGTCACGCAGCAGCGTAACTTCTTCTTCCATTGCACCAATGATGCCGATTTTCATAGATTTACTCGCGATAAGCCCGATTTGAAG  >  NC_000913/178909‑179183
                                                                                                                                        |                                                                                                                                          
ggTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGTTCCAACAGCAAAGTGGCACCCAGCGCCGCAGCGACTTTACCGATGCCCGATTTCAGAAGCGCAACCTCGGTTCCATTCAGTTTGcc                                                                                                                                         >  2:571789/1‑140 (MQ=255)
ggTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGTTCCAACAGCAAAGTGGCACCCAGCGCCGCAGCGACTTTACCGATGCCCGATTTCAGAAGCGCAACCTCGGTTCCATTCAGTTTGcc                                                                                                                                         >  1:186112/1‑140 (MQ=255)
ggTGCCAGGCCACCGGCAGAACCGGAGTTAATAATCACATCTGGCTTGCAGTGTTCCAACAGCAAAGTGGCACCCAGCGCCGCAGCGACTTTACCGATGCCCGATTTCAGAAGCGCAACCTCGGTTCCATTCAGTTTGcc                                                                                                                                         >  2:1326681/1‑140 (MQ=255)
           aCCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGTTCCAACAGCAAAGTGGCACCCAGCGCCGCAGCGACTTTACCGATGCCCGATTTCAGAAGCGCAACCTCGGTTCCATTCAGTTTGCCGGTATAGAttt                                                                                                                              >  1:691940/1‑140 (MQ=255)
               aaaaacccGGTGTTAATAACCACATCGGCCTTGCAGTGTCCCAACAGCAAAGTGCCCCCCAGCCCCGCAGCGACTTTACCGATGCCCGATTTCAGAAGCGCACCCTCGGTTCCATTCAGTTTGCCGTTATAGATTTCGCa                                                                                                                          <  2:475611/134‑1 (MQ=255)
                     ccGGTGTTAATAATCACATCTGGCTTGCAGTGTTCCAACAGCAAAGTGGCACCCAGCGCCGCAGCGACTTTACCGATGCCCGATTTCAGAAGCGCAACCTCGGTTCCATTCAGTTTGCCGGTATAGATTTCGCAAccgcc                                                                                                                    <  2:691940/140‑1 (MQ=255)
                         tgtTAATAATCACATCTGGCTTGCAGTGTTCCAACAGCAAAGTGGCACCCAGCGCCGCAGCGACTTTACCGATGCCCGATTTCAGAAGCGCAACCTCGGTTCCATTCAGTTTGCCGGTATAGATTTCGCAACCGCCgaga                                                                                                                >  1:964616/1‑140 (MQ=255)
                                   cacaTCTGGCTTGCAGTGTTCCAACAGCAAAGTGGCACCCAGCGCCGCAGCGACTTTACCGATGCCCGATTTCAGAAGCGCAACCTCGGTTCCATTCAGTTTGCCGGTATAGATTTCGCAACCGCCGAGACTGATAGttt                                                                                                      >  2:13532/1‑140 (MQ=255)
                                     caTCTGGCTTGCAGTGTTCCAACAGCAAAGTGGCACCCAGCGCCGCAGCGACTTTACCGATGCCCGATTTCAGAAGCGCAACCTCGGTTCCATTCAGTTTGCCGGTATAGATTTCGCAACCGCCGAGACTGATAGTTTGa                                                                                                    >  1:1381089/1‑140 (MQ=255)
                                       tCTGGCTTGCAGTGTTCCAACAGCAAAGTGGCACCCAGCGCCGCAGCGACTTTACCGATGCCCGATTTCAGAAGCGCAACCTCGGTTCCATTCAGTTTGCCGGTATAGATTTCGCAACCGCCGAGACTGATAGTTTGACg                                                                                                  >  2:667514/1‑140 (MQ=255)
                                       tCTGGCTTGCAGTGTTCCAACAGCAAAGTGGCACCCAGCGCCGCAGCGACTTTACCGATGCCCGATTTCAGAAGCGCAACCTCGGTTCCATTCAGTTTGCCGGTATAGATTTCGCAACCGCCGAGACTGATAGTTTGACg                                                                                                  >  1:1090382/1‑140 (MQ=255)
                                        cTGGCTTGCAGTGTTCCAACAGCAAAGTGGCACCCAGCGCCGCAGCGTCTTTACCGATGCCCGATTTCAGAAGCGCAACCTCGGTTCCATTCAGTTTGCCGGTATAGATTTCGCAACCGCCGAGACTGATAGTTTGACgg                                                                                                 >  1:1458786/1‑140 (MQ=255)
                                             ttGCAGTGTTCCAACAGCAAAGTGGCACCCAGCGCCGCAGCGACTTTACCGATGCCCGATTTCAGAAGCGCAACCTCGGTTCCATTCAGTTTGCCGGTATAGATTTCGCAACCGCCGAGACTGATAGTTTGACGGTTTTc                                                                                            >  1:970939/1‑140 (MQ=255)
                                                cAGTGTTCCAACAGCAAAGTGGCATCCAGCGCCGCAGCGACTTTACCGATGCCCGATTTCAGAAGCGCAACCTCGGTTCCATTCAGTTTGCCGGTATAGATTTCGCAACCGCCGAGACTGATAGTTTGACGGTTTTCGAt                                                                                         >  1:1336050/1‑140 (MQ=255)
                                                cAGTGTTCCAACAGCAAAGTGGCACCCAGCGCCGCAGCGACTTTACCGATGCCCGATTTCAGAAGCGCAACCTCGGTTCCATTCAGTTTGCCGGTATAGATTTCGCAACCGCCGAGACTGATAGTTTGACGGTTTTCGAt                                                                                         >  2:534934/1‑140 (MQ=255)
                                                     ttCCAACAGCAAAGTGGCACCCAGCGCCGCAGCGACTTTACCGATGCCCGATTTCAGAAGCGCAACCTCGGTTCCATTCAGTTTGCCGGTATAGATTTCGCAACCGCCGAGACTGATAGTTTGACGGTTTTCGATTTTGt                                                                                    >  2:1480526/1‑140 (MQ=255)
                                                     ttCCAACAGCAAAGTGGCACCCAGCGCCGCAGCGACTTTACCGATGCCCGATTTCAGAAGCGCAACCTCGGTTCCATTCAGTTTGCCGGTATAGATTTCGCAACCGCCGAGACTGATAGTTTGACGGTTTTCGATTTTGt                                                                                    >  2:702570/1‑140 (MQ=255)
                                                     ttCCAACAGCAAAGTGGCACCCAGCGCCGCAGCGACTTTACCGATGCCCGATTTCAGAAGCGCAACCTCGGTTCCATTCAGTTTGCCGGTATAGATTTCGCAACCGCCGAGACTGATAGTTTGACGGTTTTCGATTTTGt                                                                                    >  1:948887/1‑140 (MQ=255)
                                                          aCAGCAAAGTGGCACCCAGCGCCGCAGCGACTTTACCGATGCCCGATTTCAGAAGCGCAACCTCGGTTCCATTCAGTTTGCCGGTATAGATTTCGCAACCGCCGAGACTGATAGTTTGACGGTTTTCGATTTTGTCACgc                                                                               >  1:391282/1‑140 (MQ=255)
                                                          aCAGCAAAGTGGCACCCAGCGCCGCAGCGACTTTACCGATGCCCGATTTCAGAAGCGCAACCTCGGTTCCATTCAGTTTGCCGGTATAGATTTCGCAACCGCCGAGACTGATAGTTTGACGGTTTTCGATTTTGTCACgc                                                                               >  1:107058/1‑140 (MQ=255)
                                                             gCAAAGTGGCACCCAGCGCCGCAGCGACTTTACCGATGCCCGATTTCAGAAGCGCAACCTCGGTTCCATTCAGTTTGCCGGTATAGATTTCGCAACCGCCGAGACTGATAGTTTGACGGTTTTCGATTTTGTCACgcagc                                                                            >  2:811479/1‑140 (MQ=255)
                                                               aaaGTGGCACCCAGCGCCGCAGCGACTTTACCGATGCCCGATTTCAGAAGCGCAACCTCGGTTCCATTCAGTTTGCCGGTATAGATTTCGCAACCGCCGAGACTGATAGTTTGACGGTTTTCGATTTTGTCACGcagcag                                                                          >  1:679074/1‑140 (MQ=255)
                                                               aaaGTGGCACCCAGCGCCGCAGCGACTTTACCGATGCCCGATTTCAGAAGCGCAACCTCGGTTCCATTCAGTTTGCCGGTATAGATTTCGCAACCGCCGAGACTGATAGTTTGACGGTTTTCGATTTTGTCACGcagcag                                                                          >  1:469838/1‑140 (MQ=255)
                                                               aaaGTGGCACCCAGCGCCGCAGCGACTTTACCGATGCCCGATTTCAGAAGCGCAACCTCGGTTCCATTCAGTTTGCCGGTATAGATTTCGCAACCGCCGAGACTGATAGTTTGACGGTTTTCGATTTTGTCACGcagcag                                                                          >  1:1466776/1‑140 (MQ=255)
                                                                    ggCACCCAGCGCCGCAGCGACTTTACCGATGCCCGATTTCAGAAGCGCAACCTCGGTTCCATTCAGTTTGCCGGTATAGTTTTCGCAACCGCCGAGACTGATAGTTTGACGGTTTTCGATTTTGTCACGCAGCAGCGTaa                                                                     <  1:1085641/140‑1 (MQ=255)
                                                                      cACCCAGCGCCGCAGCGACTTTACCGATGCCCGATTTCAGAAGCGCAACCTCGGTTCCATTCAGTTTGCCGGTATAGATTTCGCAACCGCCGAGACTGATAGTTTGACGGTTTTCGATTTTGTCACGCAGCAGCGTAAct                                                                   >  1:884372/1‑140 (MQ=255)
                                                                      aaCCCAGCGCCGCAGCGACTTTACCGATGCCCGATTCCAGAAGCGCACCCTGGGTTCCTTTCAGTTTGCCGGTATAGATTCCGCAACCGCCGAGACTGATAGTTTGACGGTTTTCGATTTTGTCACGCAGCAGCGTAAct                                                                   <  2:525673/139‑1 (MQ=255)
                                                                             cgccgcAGCGACTTTACCGATGCCCGATTTCAGAAGCGCAACCTCGGTTCCATTCAGTTTGCCGGTATAGATTTCGCAACCGCCGAGACTGATAGTTTGACGGTTTTCGATTTTGTCACGCAGCAGCGTAActtcttctt                                                            >  2:471902/1‑140 (MQ=255)
                                                                                cgcAGCGATTTTCCCGAGCCCCTTTTTCAGAAGCCCACCCCCGGTTCCATTCTGTTTGCCGGTATAGTTTCCGCAACCGCCGAGACTGATAGTTTGACGGTTTTCGATTTTGTCACGCAGCAGCGTAACTTCTTCTTCCa                                                         <  2:1090382/140‑1 (MQ=255)
                                                                                cgcAGCGACTTTACCGATGCCCGATTTCAGAAGCGCAACCTCGGTTCCATTCAGTTTGCCGGTATAGATTTCGCAACCGCCGAGACTGATAGTTTGACGGTTTTCGATTTTGTCACGCAGCAGCGTAACTTCTTCTTCCa                                                         <  2:970939/140‑1 (MQ=255)
                                                                                cgcAGCGACTTTACCGATGCCCGATTTCAGAAGCGCAACCTCGGTTCCATTCAGTTTGCCGGTATAGATTTCGCAACCGCCGAGACTGATAGTTTGACGGTTTTCGATTTTGTCACGCAGCAGCGTAACTTCTTCTTCCa                                                         <  1:13532/140‑1 (MQ=255)
                                                                                agcagcaACTTTACCGGGGCCCGGTTTAAGAAGCACAACCGCGCTTCTTTTCAGTTTGCCGTTATAGATTTCGCAACCGCCGAGACTGATAGTTTGACGGTTTTCGATTTTGTCACGCAGCAGCGTAACTTCTTCTTCCa                                                         <  2:129418/139‑1 (MQ=255)
                                                                                               gATGCCCGATTTCAGAAGCGCAACCTCGGTTCCATTCAGTTTGCCGGTATAGATTTCGCAACCGCCGAGACTGATAGTTTGACGGTTTTCGATTTTGTCACGCAGCAGCGTAACTTCTTCTTCCATTGCACCAATGATGc                                          >  2:208192/1‑140 (MQ=255)
                                                                                                aTGCCCGTTTTCAGAAGCGCACCCGCGGTTCCATTCAGTTTGCCGGTATAGATTTCGCAACCGCCGAGACTGATAGTTTGACGGTTTTCGATTTTGTCACGCAGCAGCGTAACTTCTTCTTCCATTGCACCAATGATGcc                                         <  2:399521/140‑1 (MQ=255)
                                                                                                aTGCCCGATTTCAGAAGCGCAACCTCGGTTCCATTCAGTTTGCCGGTATAGATTTCGCAACCGCCGAGACTGATAGTTTGACGGTTTTCGATTTTGTCACGCAGCAGCGTAACTTCTTCTTCCATTGCACCAATGATGcc                                         >  1:210037/1‑140 (MQ=255)
                                                                                                  gCCCGATTTCAGAAGCGCAACCTCGGTTCCATTCAGTTTGCCGGTATAGATTTCGCAACCGCCGAGACTGATAGTTTGACGGTTTTCGATTTTGTCACGCAGCAGCGTAACTTCTTCTTCCATTGCACCAATGATGCCGa                                       >  2:1024368/1‑140 (MQ=255)
                                                                                                  ccccGATTTAAGAGCCACATCCTAGTTTCCATTCAGTTTGCGGTTATAGATTCCGCAACCGCCGAGACTGATAGTTTGACGGTTTTCGATTTTGTCACGCAGCAGCGTAACTTCTTCTTCCATTGCACCAATGATGCCGa                                       <  2:1458786/139‑1 (MQ=255)
                                                                                                   cccGATTTCAGAAGCGCAACCTCGGTTCCATTCAGTTTGCCGGTATAGATTTCGCAACCGCCGAGACTGATAGTTTGACGGTTTTCGATTTTGTCACGCAGCAGCGTAACTTCTTCTTCCATTGCACCAATGATGCCGAt                                      >  1:936987/1‑140 (MQ=255)
                                                                                                   cccGATTTCAGAAGCGCAACCTCGGTTCCATTCAGTTTGCCGGTATAGATTTCGCAACCGCCGAGACTGATAGTTTGACGGTTTTCGATTTTGTCACGCAGCAGCGTAACTTCTTCTTCCATTGCACCAATGATGCCGAt                                      >  2:699746/1‑140 (MQ=255)
                                                                                                                gcgcACCCTTGGTTCCATTCAGTTTGCCGTTATAGATTTCGAAACCGCCGAGACTGATAGTTTGACGGTTTTCGATTTTGTCACGCAGCAGCGTAACTTCTTCTTCCATTGCACCAATGATGCCGATTTTCATAGATTTa                         <  2:948887/140‑1 (MQ=255)
                                                                                                                         cGGTTCCATTCAGTTTGCCGGTATAGATTTCGCAACCGCCGAGACTGATAGTTTGACGGTTTTCGATTTTGTCACGCAGCAGCGTAACTTCTTCTTCCATTGCACCAATGATGCCGATTTTCATAGATTTACTCGCGATa                <  1:471902/140‑1 (MQ=255)
                                                                                                                          ggTTCAATTCTGTTTGCCGGTATAAATTTCGCAGCCGCCGAGACTGATAGTTTGACGGTTTTCGATTTTGTCACGCAGCAGCGTAACTTCTTCTTCCATTGCACCAATGATGCCGATTTTCATAGA‑TTACTCGCGATAAg              <  1:1403408/140‑1 (MQ=255)
                                                                                                                           gTTCCATTCAGTTTGCGGGTATAGATTTCGCAACCGCCGAGACTGATAGTTTGACGGTTTTCGATTTTGTCACGCAGCAGCGTAACTTCTTCTTCCATTGCACCAATGATGCCGATTTTCATAGATTTACTCGCGATAAg              <  2:1384681/140‑1 (MQ=255)
                                                                                                                             tCCATTCAGTTTGCCGGTATAGATTTCGCAACCGCCGAGACTGATAGTTTGACGGTTTTCGATTTTGTCACGCAGCAGCGTAACTTCTTCTTCCATTGCACCAATGATGCCGATTTTCATAGATTTACTCGCGATAAGcc            <  2:210037/140‑1 (MQ=255)
                                                                                                                                aTTCAGTTTGCCGGTATAGATTTCGAAACCGCCGAGACTGATAGTTTGACGGTTTTCGATTTTGTCACGCAGCAGCGTAACTTCTTCTTCCATTGCACCAATGATGCCGATTTTCATAGATTTACTCGCGATAAGCCCGa         <  1:571789/140‑1 (MQ=255)
                                                                                                                                 ttCAGTTTGCCGGTATAGATTTCGCAACCGCCGAGACTGATAGTTTGACGGTTTTCGATTTTGTCACGCAGCAGCGTAACTTCTTCTTCCATTGCACCAATGATGCCGATTTTCATAGATTTACTCGCGATAAGccc           <  1:717122/137‑1 (MQ=255)
                                                                                                                                 ttCAGTTTGCCGGTATAGATTTCGCAACCGCCGAGACTGATAGTTTGACGGTTTTCGATTTTGTCACGCAGCAGCGTAACTTCTTCTTCCATTGCACCAATGATGCCGATTTTCATAGATTTACTCGCGATAAGccc           >  2:717122/1‑137 (MQ=255)
                                                                                                                                  tCAGTTTGCCGGTATAGATTTCGCAACCGCCGAGACTGATAGTTTGACGGTTTTCGATTTTGTCACGCAGCAGCGTAACTTCTTCTTCCATTGCACCAATGATGCCGATTTTCATAGATTTACTCGCGATAAGCCCGAtt       <  2:469838/140‑1 (MQ=255)
                                                                                                                                    aGTTTGCCGGTATAGATTTCGCAACCGCCGAGACTGATAGTTTGACGGTTTTCGATTTTGTCACGCAGCAGCGTAACTTCTTCTTCCATTGCACCAATGATGCCGATTTTCATAGATTTACTCGCGATAAGCCCGATTTg     >  1:144249/1‑140 (MQ=255)
                                                                                                                                       tGGCCGGTAGAGTTTTCGAACCCGCCGAGACTGATAGTTTGACGGTTTTCGATTTTGTCACGCAGCAGCGTAACTTCTTCTTCCATTGCACCAATGATGCCGATTTTCATAGATTTACTCGCGATAAGCCCGATTTGAAg  <  2:884372/140‑1 (MQ=255)
                                                                                                                                       tGGCCGATAAATATTCCACAACCGCCGAGACTGATAGTTTGACGGTTTTCGATTTTGTCACGCAGCAGCGTAACTTCTTCTTCCATTGCACCAATGATGCCGATTTTCATAGATTTACTCGCGATAAGCCCGATTTGAAg  <  2:1381089/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                          
GGTGCCAGGCCACCGGCAGAACCGGTGTTAATAATCACATCTGGCTTGCAGTGTTCCAACAGCAAAGTGGCACCCAGCGCCGCAGCGACTTTACCGATGCCCGATTTCAGAAGCGCAACCTCGGTTCCATTCAGTTGGCCGGTATAGATTTCGCAACCGCCGAGACTGATAGTTTGACGGTTTTCGATTTTGTCACGCAGCAGCGTAACTTCTTCTTCCATTGCACCAATGATGCCGATTTTCATAGATTTACTCGCGATAAGCCCGATTTGAAG  >  NC_000913/178909‑179183

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A