Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F48 I1 R1	76	74.1	2532288	99.0%	2506965	139.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,013,784	G→T	V176L (GTG→TTG)	pqiB →	intermembrane transport protein PqiB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,013,784	0	G	T	100.0%	186.5 / NA	53	V176L (GTG→TTG)	pqiB	intermembrane transport protein PqiB
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (31/22); total (31/22)

CAGCCAGGCGCGAAAGGCAGCAAAATGGATAAATACGATTTGCTGGACTCGCCACCGTTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTAGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACTGGTGACCAACAATGTTCGCTTCTGGAAAGA  >  NC_000913/1013649‑1013920

cAGCCAGGCGCGAAAGGCAGCAAAATGGATAAATACGATTTGCTGGACTCGCCACCGTTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGTTGCt                                                                                                                                      >  2:178509/1‑140 (MQ=255)

            aaaGGCAGCAAAATGGATAAATACGATTTGCTGGACTCGCCACCGTTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGTTGCTGTTCCGTGGCTa                                                                                                                          >  2:474423/1‑140 (MQ=255)

                 cagcaAAATGGATAAATACGATTTGCTGGACTCGCCACCGTTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGTTGCTGTTCCGTGGCTATCggg                                                                                                                     >  1:1022902/1‑140 (MQ=255)

                       aaTGGATAAATACGATTTGCTGGACTCGCCACCGTTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGTTGCTGTTCCGTGGCTATCGGGTAGGtt                                                                                                               <  2:752170/140‑1 (MQ=255)

                         tGGATAAATACGATTTGCTGGACTCGCCACCGTTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGTTGCTGTTCCGTGGCTATCGGGTAGGTTCg                                                                                                             >  2:427314/1‑140 (MQ=255)

                         tGGATAAATACGATTTGCTGGACTCGCCACCGTTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGTTGCTGTTCCGTGGCTATCGGGTAGGTTCg                                                                                                             >  2:76263/1‑140 (MQ=255)

                         tGGATAAATACGATTTGCTGGACTCGCCACCGTTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGTTGCTGTTCCGTGGCTATCGGGTAGGTTCg                                                                                                             >  1:212142/1‑140 (MQ=255)

                            aTAAATACGATTTGCTGGACTCGCCACCGTTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGTTGCTGTTCCGTGGCTATCGGGTAGGTTCGGtt                                                                                                          <  2:107227/140‑1 (MQ=255)

                                 tACGATTTGCTGGACTCGCCACCGTTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGTTGCTGTTCCGTGGCTATCGGGTAGGTTCGGTTGAAAc                                                                                                     <  1:772967/140‑1 (MQ=255)

                                   agATTTGCTGGACTCGCCACCGTTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAACAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGTTGCTGTTCCGTGGCTATCGGGTAGGTTCGGTTGAAACCa                                                                                                   <  2:1031020/139‑1 (MQ=255)

                                     aTTTGCTGGACTCGCCACCGTTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGTAGATCCGTTGCTGTTCCGTGGCTATCGGGTAGGTTCGGTTGAAACCAGc                                                                                                 >  2:1209195/1‑140 (MQ=255)

                                     aTTTGCTGGACTCGCCACCGTTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGTTGCTGTTCCGTGGCTATCGGGTAGGTTCGGTTGAAACCAGc                                                                                                 >  2:1028816/1‑140 (MQ=255)

                                     aTTTGCTGGACTCGCCACCGTTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGTTGCTGTTCCGTGGCTATCGGGTAGGTTCGGTTGAAACCAGc                                                                                                 >  2:188451/1‑140 (MQ=255)

                                       ttGCTGGACTCGCCACCGTTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGTTGCTGTTCCGTGGCTATCGGGTAGGTTCGGTTGAAACCAGCAc                                                                                               <  1:1028816/140‑1 (MQ=255)

                                         gCTGGACTCGCCACCGTTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGTTGCTGTTCCGTGGCTATCGGGTAGGTTCGGTTGAAACCAGCACCt                                                                                             >  1:950673/1‑140 (MQ=255)

                                                  gCCACCGTTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGTTGCTGTTCCGTGGCTATCGGGTAGGTTCGGTTGAAACCAGCACCTTCGATacac                                                                                    >  2:1125764/1‑140 (MQ=255)

                                                  gCCACCGTTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGTTGCTGTTCCGTGGCTATCGGGTAGGTTCGGTTGAAACCAGCACCTTCGATacac                                                                                    >  1:1179152/1‑140 (MQ=255)

                                                      ccGTTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGTTGCTGTTCCGTGGCTATCGGGTAGGTTCGGTTGAAACCAGCACCTTCGATACACaaaa                                                                                <  1:427314/140‑1 (MQ=255)

                                                      ccGTTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGTTGCTGTTCCGTGGCTATCGGGTAGGTTCGGTTGAAACCAGCACCTTCGATACACaaaa                                                                                <  1:76263/140‑1 (MQ=255)

                                                           ggCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGTTGCTGTTCCGTGGCTATCGGGTAGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCa                                                                           >  2:191375/1‑140 (MQ=255)

                                                               ccGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGTTGCTGTTCCGTGGCTATCGGGTAGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAatat                                                                       <  1:1125764/140‑1 (MQ=255)

                                                               acGCCTGATGCGAAAGGTATCCGTGTTATTCTCGATAGCAAACAAGCCGGCAAGCTCTCGCCAGGAGATCCGTTTCTGTTCCGTGGCTATCGGGTAGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACACAatat                                                                       <  2:454586/139‑1 (MQ=255)

                                                                 gCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGTTGCTGTTCCGTGGCTATCGGGTAGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCa                                                                     >  1:238177/1‑140 (MQ=255)

                                                                 gCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGTTGCTGTTCCGTGGCTATCGGGTAGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCa                                                                     >  1:110573/1‑140 (MQ=255)

                                                                    agATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGTTGCTGTTCCGTGGCTATCGGGTAGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCt                                                                  <  2:950673/139‑1 (MQ=255)

                                                                       tGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGTTGCTGTTCCGTGGCTATCGGGTAGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATc                                                               <  1:868768/140‑1 (MQ=255)

                                                                         cGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGTTGCTGTTCCGTGGCTATCGGGTAGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCaa                                                             <  1:474423/140‑1 (MQ=255)

                                                                         cGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGTTGCTGTTCCGTGGCTATCGGGTAGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCaa                                                             <  2:238177/140‑1 (MQ=255)

                                                                            aaGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGTTGCTGTTCCGTGGCTATCGGGTAGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTg                                                          >  1:536109/1‑140 (MQ=255)

                                                                                      tgtgATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGTTGCTGTTCCGTGGCTATCGGGTAGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATg                                                >  2:1209924/1‑140 (MQ=255)

                                                                                      tgtgATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGTTGCTGTTCCGTGGCTATCGGGTAGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATg                                                <  2:1148495/140‑1 (MQ=255)

                                                                                      tgtgATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGTTGCTGTTCCGTGGCTATCGGGTAGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATg                                                <  2:536109/140‑1 (MQ=255)

                                                                                      tgtgATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGTTGCTGTTCCGTGGCTATCGGGTAGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATg                                                <  1:178509/140‑1 (MQ=255)

                                                                                      tgtgATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGTTGCTGTTCCGTGGCTATCGGGTAGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATg                                                <  1:1161336/140‑1 (MQ=255)

                                                                                        tgATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGTTGCTGTTCCGTGGCTATCGGGTAGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTCCATCAATGCa                                              >  2:350709/1‑140 (MQ=255)

                                                                                          aTTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGTTGCTGTTCCGTGGCTATCGGGTAGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCAcc                                            >  2:841744/1‑140 (MQ=255)

                                                                                          aTTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGTTGCTGTTCCGTGGCTATCGGGTAGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCAcc                                            >  1:1173720/1‑140 (MQ=255)

                                                                                          aTTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGTTGCTGTTCCGTGGCTATCGGGTAGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCAcc                                            >  1:1119530/1‑140 (MQ=255)

                                                                                             ctcGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGTTGCTGTTCCGTGGCTATCGGGTAGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCACCttt                                         >  2:338060/1‑139 (MQ=255)

                                                                                                gATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGTTGCTGTTCCGTGGCTATCGGGTAGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGa                                      >  2:1090127/1‑140 (MQ=255)

                                                                                                     cAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGTTGCTGTTCCGTGGCTATCGGGGAGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGAc                                 >  2:351660/1‑140 (MQ=255)

                                                                                                     cAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGTTGCTGTTCCGCGGCTATCGGGTAGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGAc                                 >  2:343683/1‑140 (MQ=255)

                                                                                                       aaaaaGCCGGGCAGCTCTCGCCAGGAGATCCGTTGCTGTTCCGTGGCTATCGGGTAGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACTg                               >  1:171663/1‑140 (MQ=255)

                                                                                                        aaaaGCCGGGCAGCTCTCGCCAGGAGATCCGTTGCTGTTCCGTGGCTATCGGGTAGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACTgg                              >  1:193089/1‑140 (MQ=255)

                                                                                                            gCCGGGCAGCTCTCGCCAGGAGATCCGTTGCTGTTCCGTGGCTATCGGGTAGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACTGGTGAc                          <  1:1090127/140‑1 (MQ=255)

                                                                                                                 gCAGCTCTCGCCAGGAGATCCGTTGCTGTTCCGTGGCTATCGGGTAGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACTGGTGACcaaca                     <  1:338060/140‑1 (MQ=255)

                                                                                                                      tctcGCCAGGAGATCCGTTGCTGTTCCGTGGCTATCGGGTAGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACTGGTGACCAACAATGtt                <  1:1002049/140‑1 (MQ=255)

                                                                                                                               gagaTCCGTTGCTGTTCCGTGGCTATCGGGTAGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACTGGTGACCAACAATGTTCGCTTCTgg       >  2:950907/1‑140 (MQ=255)

                                                                                                                               gagaTCCGTTGCTGTTCCGTGGCTATCGGGTAGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACTGGTGACCAACAATGTTCGCTTCTgg       >  2:1022906/1‑140 (MQ=255)

                                                                                                                               gagaTCCGTTGCTGTTCCGTGGCTATCGGGTAGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACTGGTGACCAACAATGTTCGCTTCTgg       >  1:568796/1‑140 (MQ=255)

                                                                                                                               gagaTCCGTTGCTGTTCCGTGGCTATCGGGTAGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACTGGTGACCAACAATGTTCGCTTCTgg       >  1:1015090/1‑140 (MQ=255)

                                                                                                                                agaTCCGTTGCTGTTCCGTGGCTATCGGGTAGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACTGGTGACCAACAATGTTCGCTTCTGGa      <  2:1179152/140‑1 (MQ=255)

                                                                                                                                agaTCCGTTGCTGTTCCGTGGCTATCGGGTAGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACTGGTGACCAACAATGTTCGCTTCTGGa      <  1:1209195/140‑1 (MQ=255)

                                                                                                                                agaTCCGTTGCTGTTCCGTGGCTATCGGGTAGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTCCATCAATGCACCTTATGACCGACTGGTGACCAACAATGTTCGCTTCTGGa      <  1:350709/140‑1 (MQ=255)

                                                                                                                                    ccGTTGCTGTTCCGTGGCTATCGGGAAGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACTGGTGACCAACAATGTTCGCTTCTGGAAAGa  >  2:566358/1‑140 (MQ=255)

CAGCCAGGCGCGAAAGGCAGCAAAATGGATAAATACGATTTGCTGGACTCGCCACCGTTGGCCCCGCCTGATGCGAAAGGTATCCGTGTGATTCTCGATAGCAAAAAAGCCGGGCAGCTCTCGCCAGGAGATCCGGTGCTGTTCCGTGGCTATCGGGTAGGTTCGGTTGAAACCAGCACCTTCGATACACAAAAACGCAATATCAGCTATCAACTGTTCATCAATGCACCTTATGACCGACTGGTGACCAACAATGTTCGCTTCTGGAAAGA  >  NC_000913/1013649‑1013920

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A