Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A16 F48 I1 R1 61 131.2 4447032 99.1% 4407008 139.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 730,515 G→T L311L (CTG→CTT)  rhsC → rhs element protein RhsC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913730,5150GT77.6% 253.1 / 91.2 125L311L (CTG→CTT) rhsCrhs element protein RhsC
Reads supporting (aligned to +/- strand):  ref base G (14/14);  new base T (52/45);  total (66/59)
Fisher's exact test for biased strand distribution p-value = 8.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 129 total aligned reads displayed.

CTGATACCCTGCCGGGTTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCGTCACACGGGCCGACCGGAA  >  NC_000913/730403‑730650
                                                                                                                |                                                                                                                                       
cTGATACCCTGCCGGGTTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTTGTGCGCTATGGCCGGACGCCCCgcggc                                                                                                              >  2:2015013/1‑140 (MQ=17)
    tACCCTGCCGGGTTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGACTTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACGCCCCGCGGCGAAc                                                                                                          >  2:1745512/1‑140 (MQ=17)
     aCCCTGCCGGGTTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACGCCCCGCGGCGAACt                                                                                                         <  2:1954306/140‑1 (MQ=32)
     aCCCTGCCGGGTTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACGCCCCGCGGCGAACt                                                                                                         >  1:1496833/1‑140 (MQ=32)
       ccTGCCGGGTTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATTCCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTgg                                                                                                       <  1:1047238/140‑1 (MQ=17)
       ccTGCCGGGTTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTTGTGCGCTATGGCTGGACGCCCCGCGGCGAACTgg                                                                                                       <  1:167644/140‑1 (MQ=18)
       ccTGCCGGGTTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTgg                                                                                                       <  2:8169/140‑1 (MQ=33)
        cTGCCGGGTTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTggc                                                                                                      <  1:164192/140‑1 (MQ=32)
         tGCCGGGTTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTTGTGCGCTATGGCTGGACGCCCCGCGGCGAACTggcg                                                                                                     >  1:1996456/1‑140 (MQ=17)
         tGCCGGGTTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTTGTGCGCTATGGCTGGACGCCCCGCGGCGAACTggcg                                                                                                     >  1:60925/1‑140 (MQ=17)
         tGCCGGGTTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTTGTGCGCTATGGCTGGACGCCCCGCGGCGAACTggcg                                                                                                     >  2:562860/1‑140 (MQ=17)
         tGCCGGGTTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTTGTGCGCTATGGCTGGACGCCCCGCGGCGAACTggcg                                                                                                     >  1:1270746/1‑140 (MQ=17)
         tGCCGGGTTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTTGTGCGCTATGGCTGGACGCCCCGCGGCGAACTggcg                                                                                                     >  2:641517/1‑140 (MQ=17)
         tGCCGGGTTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTggcg                                                                                                     >  2:377749/1‑140 (MQ=32)
         tGCCGGGTTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTggcg                                                                                                     >  2:466719/1‑140 (MQ=32)
         tGCCGGGTTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTggcg                                                                                                     >  2:515376/1‑140 (MQ=32)
         tGCCGGGTTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTggcg                                                                                                     >  2:1501934/1‑140 (MQ=32)
         tGCCGGGTTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTggcg                                                                                                     >  2:1406922/1‑140 (MQ=32)
         tGCCGGGTTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTggcg                                                                                                     >  1:930382/1‑140 (MQ=32)
         tGCCGGGTTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTggcg                                                                                                     >  2:948793/1‑140 (MQ=32)
         tGCCGGGTTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTggcg                                                                                                     >  2:1038060/1‑140 (MQ=32)
         tGCCGGGTTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTggcg                                                                                                     >  1:1616969/1‑140 (MQ=32)
          gCCGGGTTACACCGAATATGGCCGGGACAACTGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTggcgg                                                                                                    >  2:1623172/1‑140 (MQ=17)
          gCCGGGTTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGTCCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTTGTGCGCTATGGCTGGACGCCCCGCGGCGAACTggcgg                                                                                                    <  2:1810412/140‑1 (MQ=18)
          gCCGGGTTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGCCCCGGAATACCCGGAGAATTTACCTGCTGCGCCGCTTGTGCGCTATGGCTGGACGCCCCGCGGCGAACTggcgg                                                                                                    <  2:2120619/140‑1 (MQ=12)
          gCCGGGTTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATCCCCGGAGAATTTACCTGCCCCGCCGCTGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTggcgg                                                                                                    <  2:1478771/140‑1 (MQ=18)
          gCCGGGTTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTTGTGCGCTATGGCTGGACGCCCCGCGGCGAACTggcgg                                                                                                    <  1:1971121/140‑1 (MQ=18)
          gCCGGGTTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTTGTGCGCTATGGCTGGACGCCCCGCGGCGAACTggcgg                                                                                                    <  1:1937305/140‑1 (MQ=18)
          gCCGGGTTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTTGTGCGCTATGGCTGGACGCCCCGCGGCGAACTggcgg                                                                                                    <  2:1270746/140‑1 (MQ=18)
          gCCGGGTTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTTGTGCGCTATGGCTGGACGCCCCGCGGCGAACTggcgg                                                                                                    <  2:1345584/140‑1 (MQ=17)
          gCCGGGTTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTggcgg                                                                                                    <  1:595122/140‑1 (MQ=33)
          gCCGGGTTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTggcgg                                                                                                    <  1:1002070/140‑1 (MQ=33)
          gCCGGGTTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTggcgg                                                                                                    <  1:1501934/140‑1 (MQ=33)
          gCCGGGTTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTggcgg                                                                                                    <  2:362410/140‑1 (MQ=32)
          gCCGGGTTACACCGAAAATGGCCGGGACAACGGCATCCGTCTTTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTggcgg                                                                                                    <  2:1026757/140‑1 (MQ=18)
            cGGGTTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTTGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGgcggcg                                                                                                  <  1:216578/140‑1 (MQ=21)
             gggTTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTggcggtgg                                                                                                 <  1:528411/140‑4 (MQ=1)
              ggTTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTTGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGgt                                                                                                >  1:1872622/1‑140 (MQ=21)
                ttACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTTGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGgtgt                                                                                              >  2:1261053/1‑140 (MQ=21)
                       gAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTTGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCg                                                                                       <  1:520985/140‑1 (MQ=25)
                       gAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTTGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCg                                                                                       <  2:1504683/140‑1 (MQ=21)
                       aaaTATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTTGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCg                                                                                       <  2:1064107/139‑1 (MQ=21)
                         atatGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTTGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCgca                                                                                     >  1:2158740/1‑140 (MQ=37)
                         atatGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTTGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCgca                                                                                     >  1:2033594/1‑140 (MQ=37)
                         atatGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTTGTGCGCTATGGCTGGACCCCCCGCGGCGAACTCGCGGCGGAGTATGACCgca                                                                                     >  2:1682470/1‑140 (MQ=255)
                         atatGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGGGGGGGATGACCgtt                                                                                     >  2:280766/1‑138 (MQ=9)
                          tatGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTTGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCgcag                                                                                    <  2:301903/140‑1 (MQ=38)
                          tatGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTTGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCgcag                                                                                    <  2:60925/140‑1 (MQ=38)
                          aatGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTTGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCgcag                                                                                    <  2:1757352/139‑1 (MQ=255)
                             ggCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTTGTGCGCTATGGCTGGACGCCCCGCGGTGAACTGGCGGCGGTGTATGACCGCAGCaa                                                                                 >  2:1330158/1‑140 (MQ=255)
                             ggCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTTGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCaa                                                                                 >  1:428060/1‑140 (MQ=255)
                             ggCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTTGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCaa                                                                                 >  2:1099365/1‑140 (MQ=255)
                                cGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTTGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATac                                                                              >  1:964667/1‑140 (MQ=255)
                                  ggACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTTGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATacac                                                                            >  2:959949/1‑140 (MQ=255)
                                        cGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTTGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTgc                                                                      >  2:949493/1‑140 (MQ=255)
                                         ggCATCCGTCTGTCTGCCGTGTGGCTACACCTCCGCCCCGAACACCTGTTAAAATTACCTGCCCCGCCGCTTGTGCGCTATGTCTCGACGCCCCGCGGCGACCTGGCGGCGGTGTATGACCGAAGCAATACACAGGTgcc                                                                     <  2:2088461/140‑2 (MQ=9)
                                             tCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTTGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACGGGTGCGCAGc                                                                 >  2:1658983/1‑140 (MQ=255)
                                             tCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTTGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGc                                                                 >  2:555286/1‑140 (MQ=255)
                                             tCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTTGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGc                                                                 >  1:1485935/1‑140 (MQ=255)
                                             tCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTTGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGc                                                                 <  1:821943/140‑1 (MQ=255)
                                                gtctgtctGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTTGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATTGCCGCAGCAATACACAGGGGCGCAACttt                                                              >  2:1354094/1‑140 (MQ=17)
                                                gtctgtctGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTTGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCttt                                                              >  1:1472603/1‑140 (MQ=255)
                                                 tctgtctgCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTTGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTtta                                                             <  1:1261053/140‑1 (MQ=255)
                                                   tgtctgCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTTGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTttact                                                           <  2:825751/140‑1 (MQ=255)
                                                   tgtctgCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTTGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTttact                                                           >  1:222752/1‑140 (MQ=255)
                                                   tgtctgCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTTGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTttact                                                           <  2:1630766/140‑1 (MQ=255)
                                                   tgtctgCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGAGCCGCTTGTGCGCTATGGCTGGACGCCCCGCGGCGAAATGGCGGCAGTGTATGACCGCAGCAATACACAGGTGCGAAGCTttact                                                           <  1:1642685/140‑1 (MQ=17)
                                                   ggtctgCCGTGTGGCTGACCCAGGACCGGGAATACCCGGAGAATTTACCTGCCGCGCCGCTTGTGCGCTATGACTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTttact                                                           <  2:1741460/139‑1 (MQ=17)
                                                       tgCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTTGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACg                                                       <  2:2174762/140‑1 (MQ=255)
                                                       tgCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTTGTGCGCTATGGCTGGAAGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACg                                                       <  2:1485935/140‑1 (MQ=255)
                                                              tgGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTTGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATaa                                                >  1:1750546/1‑140 (MQ=255)
                                                               ggCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTTGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATaaa                                               >  1:551550/1‑140 (MQ=255)
                                                                  tGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTTGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATAc                                            <  1:2161346/140‑1 (MQ=255)
                                                                   gACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTTGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATAcc                                           <  2:1872622/140‑1 (MQ=255)
                                                                    aCGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTTGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCg                                          >  2:1637707/1‑140 (MQ=255)
                                                                       cACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTTGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCgggg                                       <  2:2033594/140‑1 (MQ=255)
                                                                            cccGGAATACCCGGAGAATTTACCTGCCGCGCCGCTTGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGa                                  <  2:428060/140‑1 (MQ=255)
                                                                            cccGGAATACCCGGAGAATTTACCTGCCGCGCCGCTTGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGa                                  <  1:959408/140‑1 (MQ=255)
                                                                                    aCCCGGAGAATTTACCTGCCGCGCCGCTTGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGgcg                          <  1:1649041/140‑1 (MQ=255)
                                                                                      ccGGAGAATTTACCTGCCGCGCCGCTTGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCa                        >  1:212082/1‑140 (MQ=255)
                                                                                      ccGGAGAATTTACCTGCCGCGCCGCTTGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCa                        >  1:1985284/1‑140 (MQ=255)
                                                                                      ccGGAGAATTTACCTGCCGCGCCGCTTGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCa                        >  1:2072007/1‑140 (MQ=255)
                                                                                      ccGGAGAATTTACCTGCCGCGCCGCTTGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCa                        >  1:229300/1‑140 (MQ=255)
                                                                                      ccGGAGAATTTACCTGCCGCGCCGCTTGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCa                        >  1:944206/1‑140 (MQ=255)
                                                                                      ccGGAGAATTTACCTGCCGCGCCGCTTGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCa                        >  1:1747921/1‑140 (MQ=255)
                                                                                      ccGGAGAATTTACCTGCCGCGCCGCTTGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCa                        >  1:870281/1‑140 (MQ=255)
                                                                                      ccGGAGAATTTACCTGCCGCGCCGCTTGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCa                        >  1:262530/1‑140 (MQ=255)
                                                                                         gagaATTTACCTGCCGCGCCGCTTGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTTTGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCg                     >  1:843142/1‑140 (MQ=255)
                                                                                         gagaATTTACCTGCCGCGCCGCTTGTGCGCTATGGCCGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCg                     >  2:2015338/1‑140 (MQ=255)
                                                                                           gaATTTACCTGCCGCGCCGCTTGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCGTc                   >  1:275088/1‑140 (MQ=255)
                                                                                              tttACCTGCCGCGCCGCTTGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCGTcaca                <  1:1705781/140‑1 (MQ=255)
                                                                                                  ccTGCCGCGCCGCTTGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACGGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCGTCACACggg            <  1:1658983/140‑1 (MQ=255)
                                                                                                    tGCCGCGCCGCTTGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCGTCACACGGGcc          <  1:129285/140‑1 (MQ=255)
                                                                                                      ccgcgccgcTTGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCGTCACACGGGCCGa        <  2:1529434/140‑1 (MQ=255)
                                                                                                      ccgcgccgcTTGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCGTCACACGGGCCGa        >  1:1179432/1‑140 (MQ=255)
                                                                                                      ccgcgccgcTTGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCGTCACACGGGCCGa        >  1:494560/1‑140 (MQ=255)
                                                                                                         cgccgcTTGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGGGGCGCACCGTCACACGGGCCGACCg     >  2:1462002/1‑140 (MQ=255)
                                                                                                            cgcTTGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCGTCACACGGGCCGACCGGaa  <  1:1209798/140‑1 (MQ=255)
                                                                                                            cgcTTGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCGTCACACGGGCCGACCGGaa  <  2:870281/140‑1 (MQ=255)
                                                                                                            cgcTTGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCGTCACACGGGCCGACCGGaa  <  2:121454/140‑1 (MQ=255)
                                                                                                                |                                                                                                                                       
CTGATACCCTGCCGGGTTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACGCCCCGCGGCGAACTGGCGGCGGTGTATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCGTCACACGGGCCGACCGGAA  >  NC_000913/730403‑730650

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A