Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A19 F48 I1 R1	83	81.6	2760494	99.1%	2735649	139.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	492,784	G→T	Q231H (CAG→CAT) Q231H (CAG→CAT)	dnaX → dnaX →	DNA polymerase III subunit tau DNA polymerase III subunit tau

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	492,784	0	G	T	98.7%	265.0 / ‑2.6	75	Q231H (CAG→CAT) Q231H (CAG→CAT)	dnaX dnaX	DNA polymerase III subunit tau DNA polymerase III subunit tau
Reads supporting (aligned to +/- strand): ref base G (0/1); new base T (32/42); total (32/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.98e-01

GCTTGAGCACATCCTCAACGAAGAACATATCGCTCACGAGCCGCGGGCGCTGCAATTGCTGGCACGCGCCGCTGAAGGCAGCCTGCGAGATGCCTTAAGTCTGACCGACCAGGCGATTGCCAGCGGTGACGGCCAGGTTTCAACCCAGGCGGTCAGTGCGATGCTGGGTACGCTTGACGACGATCAGGCGCTGTCGCTGGTTGAAGCGATGGTCGAGGCCAACGGCGAGCGCGTAATGGCGCTGATTAATGAAGCCGCTGCCCGTGGTAT  >  NC_000913/492649‑492918

gCTTGAGCACATCCTCAACGAAGAACATATCGCTCACGAGCCGCGGGCGCTGCAATTGCTGGCACGCGCCGCTGAAGGCAGCCTGCGAGATGCCTTAAGTCTGACCGACCAGGCGATTGCCAGCGGTGACGGCCATGttt                                                                                                                                    <  2:4641/140‑1 (MQ=255)

acTTGAGAACATCCTCAACGAAAAACATATCGCTCACGAGCCGCGGGCGCTGCAATTGCTGGCACGCGCCGCTGAAGGCAGCCTGCGAGATGCCTTAAGTCTGACCGACCAGGCGATTGCCAGCGGTGACGGCCATGttt                                                                                                                                    <  2:1018626/139‑1 (MQ=255)

       cacaTCCTCAACGAAGAACATATCGCTCACGAGCCGCGGGCGCTGCAATTGCTGGCACGCGCCGCTGAAGGCAGCCTGCGAGATGCCTTAAGTCTGACCGACCAGGCGATTGCCAGCGGTGACGGCCATGTTTCAACCCa                                                                                                                             >  1:386348/1‑140 (MQ=255)

       cacaTCCTCAACGAAGAACATATCGCTCACGAGCCGCGGGCGCTGCAATTGCTGGCACGCGCCGCTGAAGGCAGCCTGCGAGATGCCTTAAGTCTGACCGACCAGGCGATTGCCAGCGGTGACGGCCATGTTTCAACCCa                                                                                                                             >  2:146953/1‑140 (MQ=255)

        acaTCCTCAACGAAGAACATATCGCTTACGAGCCGCGGGCGCTGCAATTGCTGGCACGCGCCGCTGAAGGCAGCCTGCGAGATGCCTTAAGTCTGACCGACCAGGCGATTGCCAGCGGTGACGGCCATGTTTCAACCCAg                                                                                                                            <  1:833580/140‑1 (MQ=255)

         caTCCTCAACGAAGAACATATCGCTCACGAGCCGCGGGCGCTGCAATTGCTGGCACGCGCCGCTGAAGGCAGCCTGCGAGATGCCTTAAGTCTGACCGACCAGGCGATTGCCAGCGGTGACGGCCATGTTTCaa                                                                                                                                 <  2:1231271/134‑1 (MQ=255)

         caTCCTCAACGAAGAACATATCGCTCACGAGCCGCGGGCGCTGCAATTGCTGGCACGCGCCGCTGAAGGCAGCCTGCGAGATGCCTTAAGTCTGACCGACCAGGCGATTGCCAGCGGTGACGGCCATGTTTCaa                                                                                                                                 >  1:1231271/1‑134 (MQ=255)

         caTCCTCAACGAAGAACATATCGCTCACGAGCCGCGGGCGCTGCAATTGCTGGCACGCGCCGCTGAAGGCAGCCTGCGAGATGCCTTAAGTCTGACCGACCAGGCGATTGCCAGCGGTGACGGCCATGTTTCAACCCAgg                                                                                                                           >  2:957174/1‑140 (MQ=255)

           tCCTCAACGAAGAACATATCGCTCACGAGCCGCGGGCGCTGCAATTGCTGGCACGCGCCGCTGAAGGCAGCCTGCGAGATGCCTTAAGTCTGACCGACCAGGCGATTGCCAGTGGTGACGGCCATGTTTCAACCCAGGCg                                                                                                                         <  1:1118815/140‑1 (MQ=255)

             cTCAACGAAGAACATATCGCTCACGAGCCGCGGGCGCTGCAATTGCTGGCACGCGCCGCTGAAGGCAGCCTGCGAGATGCCTTAAGTCTGACCGACCAGGCGATTGCCAGCGGTGACGGCCATGTTTCAACCCAGGCGGt                                                                                                                       <  1:12037/140‑1 (MQ=255)

             cTCAACGAAGAACATATCGCTCACGAGCCGCGGGCGCTGCAATTGCTGGCACGCGCCGCTGAAGGCAGCCTGCGAGATGCCTTAAGTCTGACCGACCAGGCGATTGCCAGCGGTGACGGCCATGTTTCAACCCAGGCGGt                                                                                                                       <  2:363042/140‑1 (MQ=255)

             cTCAACGAAGAACATATCGCTCACGAGCCGCGGGCGCTGCAATTGCTGGCACGCGCCGCTGAAGGCAGCCTGCGAGATGCCTTAAGTCTGACCGACCAGGCGATTGCCAGCGGTGACGGCCATGTTTCAACCCAGGCGGt                                                                                                                       <  2:652955/140‑1 (MQ=255)

                  cGAAGAACATATCGCTCACGAGCCGCGGGCGCTGCAATTGCTGGCACGCGCCGCTGAAGGCAGCCTGCGAGATGCCTTAAGTCTGACCGACCAGGCGATTGCCAGCGGTGACGGCCATGTTTCAACCCAGGCGGTCAGTg                                                                                                                  <  1:83928/140‑1 (MQ=255)

                  cGAAGAACATATCGCTCACGAGCCGCGGGCGCTGCAATTGCTGGCACGCGCCGCTGAAGGCAGCCTGCGAGATGCCTTAAGTCTGACCGACCAGGCGATTGCCAGCGGTGACGGCCATGTTTCAACCCAGGCGGTCAGTg                                                                                                                  <  1:1024346/140‑1 (MQ=255)

                      gaaCATATCGCTCACGAGCCGCGGGCGCTGCAATTGCTGGCACGCGCCGCTGAAGGCAGCCTGCGAGATGCCTTAAGTCTGACCGACCAGGCGATTGCCAGCGGTGACGGCCATGTTTCAACCCAGGCGGTCAGTGCGAt                                                                                                              >  2:714061/1‑140 (MQ=255)

                      gaaCATATCGCTCACGAGCCGCGGGCGCTGCAATTGCTGGCACGCGCCGCTGAAGGCAGCCTGCGAGATGCCTTAAGTCTGACCGACCAGGCGATTGCCAGCGGTGACGGCCAGGTTTCAACCCAGGCGGTCAGTGCGAt                                                                                                              <  1:618143/140‑1 (MQ=255)

                       aaCATATCGCTCACGAGCCGCGGGCGCTGCAATTGCTGGCACGCGCCGCTGAAGGCAGCCTGCGAGATGCCTTAAGTCTGACCGACCAGGCGATTGCCAGCGGTGACGGCCATGTTTCAACCCAGGCGGTCAGTGCGATg                                                                                                             <  2:1102798/140‑1 (MQ=255)

                       aaCATATCGCTCACGAGCCGCGGGCGCTGCAATTGCTGGCACGCGCCGCTGAAGGCAGCCTGCGAGATGCCTTAAGTCTGACCGACCAGGCGATTGCCAGCGGTGACGGCCATGTTTCAACCCAGGCGGTCAGTGCGATg                                                                                                             >  1:1211613/1‑140 (MQ=255)

                        aCATATCGCTCACGAGCCGCGGGCGCTGCAATTGCTGGCACGCGCCGCTGAAGGCAGCCTGCGAGATGCCTTAAGTCTGACCGACCAGGCGATTGCCAGCGGTGACGGCCATGTTTCAACCCAGGCGGTCAGTGCGATGc                                                                                                            >  2:263239/1‑140 (MQ=255)

                            atCGCTCACGAGCCGCGGGCGCTGCAATTGCTGGCACGCGCCGCTGAAGGCAGCCTGCGAGATGCCTTAAGTCTGACCGACCAGGCGATTGCCAGCGGTGACGGCCATGTTTCAACCCAGGCGGTCAGTGCGATGCTggg                                                                                                        <  2:1378806/140‑1 (MQ=255)

                            atCGCTCACGAGCCGCGGGCGCTGCAATTGCTGGCACGCGCCGCTGAAGGCAGCCTGCGAGATGCCTTAAGTCTGACCGACCAGGCGATTGCCAGCGGTGACGGCCATGTTTCAACCCAGGCGGTCAGTGCGATGCTggg                                                                                                        >  2:945315/1‑140 (MQ=255)

                            atCGCTCACGAGCCGCGGGCGCTGCAATTGCTGGCACGCGCCGCTGAAGGCAGCCTGCGAGATGCCTTAAGTCTGACCGACCAGGCGATTGCCAGCGGTGACGGCCATGTTTCAACCCAGGCGGTCAGTGCGATGCTggg                                                                                                        >  1:1378806/1‑140 (MQ=255)

                             tCGCTCCCGAGCCGCGGGCGCTGCAATTGCTGGCCGGCGGCGCTGAAGGCAGCCTGCGAGATGGCTTAAGTCTGACCGACCAGGCGATTGCCAGCGGTGACGGCCATGTTTCAACCCAGGCGGTCAGTGCGATGCTGGGt                                                                                                       <  2:225609/140‑1 (MQ=255)

                             tCGCTCACGAGCCGCGGGCGCTGCAATTGCTGGCACGCGCCGCTGAAGGCAGCCTGCGAGATGCCTTAAGTCTGACCGACCAGGCGATTGCCAGCGGTGACGGCCATGTTTCAACCCAGGCGGTCAGTGCGATGCTGGGt                                                                                                       <  1:750280/140‑1 (MQ=255)

                             acGCTCACGAGCCGCGGGCGCTGCAATTGCTGGCACGCGCCGCTGAAGGCAGCCTGCGAGATGCCTTAAGTCTGACCGACCAGGCGATTGCCAGCGGTGACGGCCATGTTTCAACCCAGGCGGTCAGTGCGATGCTGGGt                                                                                                       <  2:648876/139‑1 (MQ=255)

                               gCTCACGAGCCGCGGGCGCTGCAATTGCTGGCACGCGCCGCTGAAGGCAGCCTGCGAGATGCCTTAAGTCTGACCGACCAGGCGATTGCCAGCGGTGACGGCCATGTTTCAACCCAGGCGGTCAGTGCGATGCTGGGTAc                                                                                                     >  2:557088/1‑140 (MQ=255)

                                 tCACGAGCCGCGGGCGCTGCAATTGCTGGCACGCGCCGCTGAAGGCAGCCTGCGAGATGCCTTAAGTCTGACCGACCAGGCGATTGCCAGCGGTGACGGCCATGTTTCAACCCAGGCGGTCAGTGCGATGCTGGGTACGc                                                                                                   >  1:265356/1‑140 (MQ=255)

                                 tCACGAGCCGCGGGCGCTGCAATTGCTGGCACGCGCCGCTGAAGGCAGCCTGCGAGATGCCTTAAGTCTGACCGACCAGGCGATTGCCAGCGGTGACGGCCATGTTTCAACCCAGGCGGTCAGTGCGATGCTGGGTACGc                                                                                                   <  1:1192546/140‑1 (MQ=255)

                                 tCACGAGCCGCGGGCGCTGCAATTGCTGGCACGCGCCGCTGAAGGCAGCCTGCGAGATGCCTTAAGTCTGACCGACCAGGCGATTGCCAGCGGTGACGGCCATGTTTCAACCCAGGCGGTCAGTGCGATGCTGGGTACGc                                                                                                   >  1:977564/1‑140 (MQ=255)

                                 tCACGAGCCGCGGGCGCTGCAATTGCTGGCACGCGCCGCTGAAGGCAGCCTGCGAGATGCCTTAAGTCTGACCGACCAGGCGATTGCCAGCGGTGACGGCCATGTTTCAACCCAGGCGGTCAGTGCGATGCTGGGTACGc                                                                                                   >  2:205117/1‑140 (MQ=255)

                                   gcGAGCCGCGGGCGCTGCAATTGCTGGCACGCGCCGCTGAAGGCAGCCTGCGCGCTTCCTTAACTCTGTCCGACCAAGCGTTTGCCAGTTTTGACGGCCATGTTTCAACCCAGGCGCTCAGTGCTATGCTGGGTACGCtt                                                                                                 >  2:1299821/2‑140 (MQ=255)

                                    caaGCCGCGGGCGCTGCAATTGCTGGCACGCGCCGCTGAAGGCAGCCTGCGAGATGCCTTAAGTCTGACCGACCAGGCGATTGCCAGCGGTGACGGCCATGTTTCAACCCAGGCGGTCAGTGCGATGCTGGGTACGCTTg                                                                                                >  2:193311/3‑140 (MQ=255)

                                    cGAGCCGCGGGCGCTGCAATTGCTGGCACGCGCCGCTGAAGGCAGCCTGCGAGATGCCTTAAGTCTGACCGACCAGGCGATTGCCAGCGGTGACGGCCATGTTTCAACCCAGGCGGTCAGTGCGATGCTGGGTACGCTTg                                                                                                >  1:695749/1‑140 (MQ=255)

                                        ccGCGGGCGCTGCAATTGCTGGCACGCGCCGCTGAAGGCAGCCTGCGAGATGCCTTAAGTCTGACCGACCAGGCGATTGCCAGCGGTGACGGCCATGTTTCAACCCAGGCGGTCAGTGCGATGCTGGGTACGCTTgacga                                                                                            <  1:982155/140‑1 (MQ=255)

                                        ccGCGGGCGCTGCAATTGCTGGCACGCGCCGCTGAAGGCAGCCTGCGAGATGCCTTAAGTCTGACCGACCAGGCGATTGCCAGCGGTGACGGCCATGTTTCAACCCAGGCGGTCAGTGCGATGCTGGGTACGCTTgacga                                                                                            <  2:983666/140‑1 (MQ=255)

                                           cgGGCGCTGCAATTGCTGGCACGCGCCGCTGAAGGCAGCCTGCGAGATGCCTTAAGTCTGACCGACCAGGCGATTGCCAGCGGTGACGGCCATGTTTCAACCCAGGCGGTCAGTGCGATGCTGGGTACGCTTGAcgacga                                                                                         >  1:1346915/1‑140 (MQ=255)

                                                 cTGCAATTGCTGGCACGCGCCGCTGAAGGCAGCCTGCGAGATGCCTTAAGTCTGACCGACCAGGCGATTGCCAGCGGTGACGGCCATGTTTCAACCCAGGCGGTCAGTGCGATGCTGGGTACGCTTGACGACGATCAGgc                                                                                   >  2:61007/1‑140 (MQ=255)

                                                  tGCAATTGCTGGCACGCGCCGCTGAAGGCAGCCTGCGAGATGCCTTAAGTCTGACCGACCAGGCGATTGCCAGCGGTGACGGCCATGTTTCAACCCAGGCGGTCAGTGCGATGCTGGGTACGCTTGACGACGATCAGgcg                                                                                  <  1:19790/140‑1 (MQ=255)

                                                   gCAATTGCTGGCACGCGCCGCTGAAGGCAGCCTGCGAGATGCCTTAAGTCTGACCGACCAGGCGATTGCCAGCGGTGACGGCCATGTTTCAACCCAGGCGGTCAGTGCGATGCTGGGTACGCTTGACGACGATCAGgcgc                                                                                 <  1:584152/140‑1 (MQ=255)

                                                   gCAATTGCTGGCACGCGCCGCTGAAGGCAGCCTGCGAGATGCCTTAAGTATGACCGACCAGGCGATTGCCAGCGGTGACGGCCATGTTTCAACCCAGGCGGTCAGTGCGATGCTGGGTACGCTTGACGACGATCAGgcgc                                                                                 >  2:1095380/1‑140 (MQ=255)

                                                        tGCTGGCACGCGCCGCTGAAGGCAGCCTGCGAGATGCCTTAAGTCTGACCGACCAGGCGATTGCCAGCGGTGACGGCCATGTTTCAACCCAGGCGGTCAGTGCGATGCTGGGTACGCTTGACGACGATCAGGCGCTGTCg                                                                            <  1:1118657/140‑1 (MQ=255)

                                                         gCTGGCACGCGCCGCTGAAGGCAGCCTGCGAGATGCCTTAAGTCTGACCGACCAGGCGATTGCCAGCGGTGACGGCCATGTTTCAACCCAGGCGGTCAGTGCGATGCTGGGTACGCTTGACGACGATCAGGCGCTGTCGc                                                                           >  2:872587/1‑140 (MQ=255)

                                                            ggCACGCGCCGCTGAAGGCAGCCTGCGAGATGCCTTAAGTCTGACCGACCAGGCGATTGCCAGCGGAGACGGCCATGTTTCAACCCAGGCGGTCAGTGCGATGCTGGGTACGCTTGACGACGATCAGGCGCTGTCGCTgg                                                                        >  2:1293538/1‑140 (MQ=255)

                                                              cACGCGCCGCTGAAGGCAGCCTGCGAGATGCCTTAAGTCTGACCGACCAGGCGATTGCCAGCGGTGACGGCCATGTTTCAACCCAGGCGGTCAGTGCGATGCTGGGTACGCTTGACGACGATCAGGCGCTGTCGCTGGtt                                                                      >  1:755718/1‑140 (MQ=255)

                                                                     cgcTGAAGGCAGCCTGCGAGATGCCTTAAGTCTGACCGACCAGGCGATTGCCAGCGGTGACGGCCATGTTTCAACCCAGGCGGTCAGTGCGATGCTGGGTACGCTTGACGACGATCAGGCGCTGTCGCTGGTTGAAGCGa                                                               <  2:1220582/140‑1 (MQ=255)

                                                                     cgcTGAAGGCAGCCTGCGAGATGCCTTAAGTCTGACCGACCAGGCGATTGCCAGCGGTGACGGCCATGTTTCAACCCAGGCGGTCAGTGCGATGCTGGGTACGCTTGACGACGATCAGGCGCTGTCGCTGGTTGAAGCGa                                                               <  1:557088/140‑1 (MQ=255)

                                                                          aaGGCAGCCTGCGAGATGCCTTAAGTCTGACCGACCAGGCGATTGCCAGCGGTGACGGCCATGTTTCAACCCAGGCGGTCAGTGCGATGCTGGGTACGCTTGACGACGATCAGGCGCTGTCGCTGGTTGAAGCGATGGTc                                                          <  2:1234942/140‑1 (MQ=255)

                                                                          aaGGCAGCCTGCGAGATGCCTTAAGTCTGACCGACCAGGCGATTGCCAGCGGTGACGGCCATGTTTCAACCCAGGCGGTCAGTGCGATGCTGGGTACGCTTGACGACGATCAGGCGCTGTCGCTGGTTGAAGCGATGGTc                                                          <  1:945315/140‑1 (MQ=255)

                                                                          aaGGCAGCCTGCGAGATGCCTTAAGTCTGACCGACCAGGCGATTGCCAGCGGTGACGGCCATGTTTCAACCCAGGCGGTCAGTGCGATGCTGGGTACGCTTGACGACGATCAGGCGCTGTCGCTGGTTGAAGCGATGGTc                                                          <  2:650163/140‑1 (MQ=255)

                                                                                      gagaTGCCTTAAGTCTGACCGACCAGGCGATTGCCAGCGGTGACGGCCATGTTTCAACCCAGGCGGTCAGTGCGATGCTGGGTACGCTTGACGACGATCAGGCGCTGTCGCTGGTTGAAGCGATGGTCGAGGCCAACGGc                                              <  1:205117/140‑1 (MQ=255)

                                                                                        gaTGCCTTAAGTCTGACCGACCAGGCGATTGCCAGCGGTGACGGCCATGTTTCAACCCAGGCGGTCAGTGCGATGCTGGGTACGCTTGACGACGATCAGGCGCTGTCGCTGGTTGAAGCGATGGTCGAGGCCAACGGCGa                                            <  2:755718/140‑1 (MQ=255)

                                                                                        gaTGCCTTAAGTCTGACCGACCAGGCGATTGCCAGCGGTGACGGCCATGTTTCAACCCAGGCGGTCAGTGCGATGCTGGGTACGCTTGACGACGATCAGGCGCTGTCGCTGGTTGAAGCGATGGTCGAGGCCAACGGCGa                                            <  1:714061/140‑1 (MQ=255)

                                                                                        gaTGCCTTAAGTCTGACCGACCAGGCGATTGCCAGCGGTGACGGCCATGTTTCAACCCAGGCGGTCAGTGCGATGCTGGGTACGCTTGACGACGATCAGGCGCTGTCGCTGGTTGAAGCGATGGTCGAGGCCAACGGCGa                                            <  1:61007/140‑1 (MQ=255)

                                                                                           gCCTTAAGTCTGACCGACCAGGCGATTGCCAGCGGTGACGGCCATGTTTCAACCCAGGCGGTCAGTGCGATGCTGGGTACGCTTGACGACGATCAGGCGCTGTCGCTGGTTGAAGCGATGGTCGAGGCCAACGGCGAgcg                                         >  2:546927/1‑140 (MQ=255)

                                                                                           gCCTTAAGTCTGACCGACCAGGCGATTGCCAGCGGTGACGGCCATGTTTCAACCCAGGCGGTCAGTGCGATGCTGGGTACGCTTGACGACGATCAGGCGCTGTCGCTGGTTGAAGCGATGGTCGAGGCCAACGGCGAgcg                                         >  1:32099/1‑140 (MQ=255)

                                                                                                aaGTCTGACCGACCAGGCGATTGCCAGCGGTGACGGCCATGTTTCAACCCAGGCGGTCAGTGCGATGCTGGGTACGCTTGACGACGATCAGGCGCTGTCGCTGGTTGAAGCGATGGTCGAGGCCAACGGCGAGCGCGTaa                                    <  2:956141/140‑1 (MQ=255)

                                                                                                aaGTCTGACCGACCAGGCGATTGCCAGCGGTGACGGCCATGTTTCAACCCAGGCGGTCAGTGCGATGCTGGGTACGCTTGACGACGATCAGGCGCTGTCGCTGGTTGAAGCGATGGTCGAGGCCAACGGCGAGCGCGTaa                                    <  1:872587/140‑1 (MQ=255)

                                                                                                     tGACCGACCAGGCGATTGCCAGCGGTGACGGCCATGTTTCAACCCAGGCGGTCAGTGCGATGCTGGGTACGCTTGACGACGATCAGGCGCTGTCGCTGGTTGAAGCGATGGTCGAGGCCAACGGCGAGCGCGTAATGgcg                               <  2:1140513/140‑1 (MQ=255)

                                                                                                     tGACCGACCAGGCGATTGCCAGCGGTGACGGCCATGTTTCAACCCAGGCGGTCAGTGCGATGCTGGGTACGCTTGACGACGATCAGGCGCTGTCGCTGGTTGAAGCGATGGTCGAGGCCAACGGCGAGCGCGTAATGgcg                               <  2:977564/140‑1 (MQ=255)

                                                                                                     tGACCGACCAGGCGATTGCCAGCGGTGACGGCCATGTTTCAACCCAGGCGGTCAGTGCGATGCTGGGTACGCTTGACGACGATCAGGCGCTGTCGCTGGTTGAAGCGATGGTCGAGGCCAACGGCGAGCGCGTAATGgcg                               <  1:957174/140‑1 (MQ=255)

                                                                                                     tGACCGACCAGGCGATTGCCAGCGGTGACGGCCATGTTTCAACCCAGGCGGTCAGTGCGATGCTGGGTACGCTTGACGACGATCAGGCGCTGTCGCTGGTTGAAGCGATGGTCGAGGCCAACGGCGAGCGCGTAATGgcg                               <  2:148957/140‑1 (MQ=255)

                                                                                                      gaccgaccAGGCGATTGCCAGCGGTGACGGCCATGTTTCAACCCAGGCGGTCAGTGCGATGCTGGGTACGCTTGACGACGATCAGGCGCTGTCGCTGGTTGAAGCGATGGTCGAGGCCAACGGCGAGCGCGTAATGgcgc                              >  2:929221/1‑140 (MQ=255)

                                                                                                       accgaccAGGCGATTGCCAGCGGTGACGGCCATGTTTCAACCCAGGCGGTCAGTGCGATGCTGGGTACGCTTGACGACGATCAGGCGCTGTCGCTGGTTGAAGCGATGGTCGAGGCCAACGGCGAGCGCGTAATGgcgc                              >  1:645536/1‑139 (MQ=255)

                                                                                                       accgaccAGGCGATTGCCAGCGGTGACGGCCATGTTTCAACCCAGGCGGTCAGTGCGATGCTGGGTACGCTTGACGACGATCAGGCGCTGTCGCTGGTTGAAGCGATGGTCGAGGCCAACGGCGAGCGCGTAATGgcgc                              <  2:645536/139‑1 (MQ=255)

                                                                                                       accgaccAGGCGATTGCCAGCGGTGACGGCCATGTTTCAACCCAGGCGGTCAGTGCGATGCTGGGTACGCTTGACGACGATCAGGCGCTGTCGCTGGTTGAAGCGATGGTCGAGGCCAACGGCGAGCGCGTAATGGTGCt                             >  1:294193/1‑140 (MQ=255)

                                                                                                       accgaccAGGCGATTGCCAGCGGTGACGGCCATGTTTCAACCCAGGCGGTCAGTGCGATGCTGGGTACGCTTGACGACGATCAGGCGCTGTCGCTGGTTGAAGCGATGGTCGAGGCCAACGGCGAGCGCGTAATGGCGCt                             >  2:619471/1‑140 (MQ=255)

                                                                                                       accgaccAGGCGATTGCCAGCGGTGACGGCCATGTTTCAACCCAGGCGGTCAGTGCGATGCTGGGTACGCTTGACGACGATCAGGCGCTGTCGCTGGTTGAAGCGATGGTCGAGGCCAACGGCGAGCGCGTAATGGCGCt                             >  1:211784/1‑140 (MQ=255)

                                                                                                       accgaccAGGCGATTGCCAGCGGTGACGGCCATGTTTCAACCCAGGCGGTCAGTGCGATGCTGGGTACGCTTGACGACGATCAGGCGCTGTCGCTGGTTGAAGCGATGGTCGAGGCCAACGGCGAGCGCGTAATGGCGCt                             >  1:1367351/1‑140 (MQ=255)

                                                                                                             cAGGCGATTGCCAGCGGTGACGGCCATGTTTCAACCCAGGCGGTCAGTGCGATGCTGGGTACGCTTGACGACGATCAGGCGCTGTCGCTGGTTGAAGCGATGGTCGAGGCCAACGGCGAGCGCGTAATGGCGCTGATTaa                       >  2:79997/1‑140 (MQ=255)

                                                                                                                gCGATTGCCAGCGGTGACGGCCATGTTTCAACCCAGGCGGTCAGTGCGATGCTGGGTACGCTTGACGACGATCAGGCGCTGTCGCTGGTTGAAGCGATGGTCGAGGCCAACGGCGAGCGCGTAATGGCGCTGATTAATGa                    <  1:193311/140‑1 (MQ=255)

                                                                                                                    ttGCCAGCGGTGACGGCCATGTTTCAACCCAGGCGGTCAGTGCGATGCTGGGTACGCTTGACGACGATCAGGCGCTGTCGCTGGTTGAAGCGATGGTCGAGGCCAACGGCGAGCGCGTAATGGCGCTGATTAATGAAGcc                <  2:1346915/140‑1 (MQ=255)

                                                                                                                      gCCAGCGGTGACGGCCATGTTTCAACCCAGGCGGTCAGTGCGATGCTGGGTACGCTTGACGACGATCAGGCGCTGTCGCTGGTTGAAGCGATGGTCGAGGCCAACGGCGAGCGCGTAATGGCGCTGATTAATGAAGCCGc              >  1:739798/1‑140 (MQ=255)

                                                                                                                       ccAGCGGTGACGGCCATGTTTCAACCCAGGCGGTCAGTGCGATGCTGGGTACGCTTGACGACGATCAGGCGCTGTCGCTGGTTGAAGCGATGGTCGAGGCCAACGGCGAGCGCGTAATGGCGCTGATTAATGAAGCCGCt             <  1:1192599/140‑1 (MQ=255)

                                                                                                                           gggTGACGGCCATGTTTCAACCCAGGCGGTCAGTGCGATGCTGGGTACGCTTGACGACGATCAGGCGCTGTCGCTGGTTGAAGCGATGGTCGAGGCCAACGGCGAGCGCGTAATGGCGCTGATTAATGAAGCCGCTGccc         <  2:1111263/139‑1 (MQ=255)

                                                                                                                                aCGGCCATGTTTCAACCCAGGCGGTCAGTGCGATGCTGGGTACGCTTGACGACGATCAGGCGCTGTCGCTGGTTGAAGCGATGGTCGAGGCCAACGGCGAGCGCGTAATGGCGCTGATTAATGAAGCCGCTGCCCGTGGt    >  2:33312/1‑140 (MQ=255)

                                                                                                                                  ggCCATGTTTCAACCCAGGCGGTCAGTGCGATGCTGGGTACGCTTGACGACGATCAGGCGCTGTCGCTGGTTGAAGCGATGGTCGAGGCCAACGGCGAGCGCGTAATGGCGCTGATTAATGAAGCCGCTGCCCGTGGTAt  <  1:146953/140‑1 (MQ=255)

GCTTGAGCACATCCTCAACGAAGAACATATCGCTCACGAGCCGCGGGCGCTGCAATTGCTGGCACGCGCCGCTGAAGGCAGCCTGCGAGATGCCTTAAGTCTGACCGACCAGGCGATTGCCAGCGGTGACGGCCAGGTTTCAACCCAGGCGGTCAGTGCGATGCTGGGTACGCTTGACGACGATCAGGCGCTGTCGCTGGTTGAAGCGATGGTCGAGGCCAACGGCGAGCGCGTAATGGCGCTGATTAATGAAGCCGCTGCCCGTGGTAT  >  NC_000913/492649‑492918

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A