Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A18 F48 I1 R1	70	76.3	2572620	98.7%	2539175	139.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	549,903	C→A	R93S (CGT→AGT)	ylbF →	DUF2877 domain‑containing protein YlbF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	549,903	0	C	A	100.0%	216.8 / NA	62	R93S (CGT→AGT)	ylbF	DUF2877 domain‑containing protein YlbF
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (35/27); total (35/27)

TGGTTTCGGCCCCGGAGGATGGGTGCTTCGCCGTGCGCAATTCGATGCGTTATGCGGTGGATTATGCGGCAATGAACGACCACAGGTTGTGGCTCAAGGGATTCGCCTCGGGCGTTTCACGGTTAAACAGCCACAGCGTTATTGTTTGCTGCGTATTACGCCGCCTGCGCATCCTCAACCACTTGCAGCTGCATGGATGCAACGCGCGGAGGAAACCGGGCTTTTCGGGCCACTGGCGTTGGCGGCAAGCGATCCGCTGCCTGCTGAGTT  >  NC_000913/549767‑550036

tGGTTTCGGCCCCGGAGGATGGGTGCTTCGCCGTGCGCAATTCGATGCGTTATGCGGTGGATTATGCGGCAATGAACGACCACAGGTTGTGGCTCAAGGGATTCGCCTCGGGCGTTTCACGGTTAAACAGCCACAGAGtt                                                                                                                                    >  2:835680/1‑140 (MQ=255)

  gTTTCGGCCCCGGAGGATGGGTGCTTCGCCGTGCGCAATTCGATGCGTTATGCGGTGGATTATGCGGCAATGAACGACCACAGGTTGTGGCTCAAGGGATTCGCCTCGGGCGTTTCACGGTTAAACAGCCACAGAGTTAt                                                                                                                                  >  1:436436/1‑140 (MQ=255)

     tCGGCCCCGGAGGATGGGTGCTTCGCCGTGCGCAATTCGATGCGTTATGCGGTGGATTATGCGGCAATGAACGACCACAGGTTGTGGCTCAAGGGATTCGCCTCGGGCGTTTCACGGTTAAACAGCCACAGAGTTATTGt                                                                                                                               >  1:823112/1‑140 (MQ=255)

     tCGGCCCCGGAGGATGGGTGCTTCGCCGTGCGCAATTCGATGCGTTATGCGGTGGATTATGCGGCAATGAACGACCACAGGTTGTGGCTCAAGGGATTCGCCTCGGGCGTTTCACGGTTAAACAGCCACAGAGTTATTGt                                                                                                                               >  1:1194049/1‑140 (MQ=255)

     tCGGCCCCGGAGGATGGGTGCTTCGCCGTGCGCAATTCGATGCGTTATGCGGTGGATTATGCGGCAATGAACGACCACAGGTTGTGGCTCAAGGGATTCGCCTCGGGCGTTTCACGGTTAAACAGCCACAGAGTTATTGt                                                                                                                               >  2:1191343/1‑140 (MQ=255)

       ggCCCCGGAGGATGGGTGCTTCGCCGTGCGCAATTCGATGCGTTATGCGGTGGATTATGCGGCAATGAACGACCACAGGTTGTGGCTCAAGGGATTCGCCTCGGGCGTTTCACGGTTAAACAGCCACAGAGTTATTGttt                                                                                                                             >  2:551345/1‑140 (MQ=255)

          cccGGAGGATGGGTGCTTCGCCGTGCGCAATTCGATGCGTTATGCGGTGGATTATGCGGCAATGAACGACCACAGGTTGTGGCTCAAGGGATTCGCCTCGGGCGTTTCACGGTTAAACAGCCACAGAGTTATTGTTtgct                                                                                                                          <  1:835680/140‑1 (MQ=255)

          cccGGAGGATGGGTGCTTCGCCGTGCGCAATTCGATGCGTTATGCGGTGGATTATGCGGCAATGAACGACCACAGGTTGTGGCTCAAGGGATTCGCCTCGGGCGTTTCACGGTTAAACAGCCACAGAGTTATTGTTtgct                                                                                                                          <  2:22279/140‑1 (MQ=255)

          cccGGAGGATGGGTGCTTCGCCGTGCGCAATTCGATGCGTTATGCGGTGGATTATGCGGCAATGAACGACCACAGGTTGTGGCTCAAGGGATTCGCCTCGGGCGTTTCACGGTTAAACAGCCACAGAGTTATTGTTtgct                                                                                                                          <  1:313329/140‑1 (MQ=255)

            cggcggATGGGTGCCTCGCCGTGCGCAATTCGATGCGTTATGCGGTGGATTATGCGGCAATGAACGACCACAGGTTGTGGCTCAAGGGATTCGCCTCGGGCGTTTCACGGTTAAACAGCCACAGAGGTATTGTTtgctgc                                                                                                                        >  2:200763/1‑140 (MQ=255)

              gaggaTGGGTGCTTCGCCGTGCGCAATTCGATGCGTTATGCGGTGGATTATGCGGCAATGAACGACCACAGGTTGTGGCTCAAGGGATTCGCCTCGGGCGTTTCACGGTTAAACAGCCACAGAGTTATTGTTTGCTGCGt                                                                                                                      <  1:836826/140‑1 (MQ=255)

                    gggTGCTTCGCCGTGCGCAATTCGATGCGTTATGCGGTGGATTATGCGGCAATGAACGACCACAGGTTGTGGCTCAAGGGATTCGCCTCGGGCGTTTCACGGTTAAACAGCCACAGAGTTATTGTTTGCTGCGTATTAcg                                                                                                                <  2:584440/140‑1 (MQ=255)

                    gggTGCTTCGCCGTGCGCAATTCGATGCGTTATGCGGTGGATTATGCGGCAATGAACGACCACAGGTTGTGGCTCAAGGGATTCGCCTCGGGCGTTTCACGGTTAAACAGCCACAGAGTTATTGTTTGCTGCGTATTAcg                                                                                                                <  2:1244051/140‑1 (MQ=255)

                       tGCTTCGCCGTGCGCAATTCGATGCGTTATGCGGTGGATTATGCGGCAATGAACGACCACAGGTTGTGGCTCAAGGGATTCGCCTCGGGCGTTTCACGGTTAAACAGCCACAGAGTTATTGTTTGCTGCGTATTAcgccg                                                                                                             <  1:1094091/140‑1 (MQ=255)

                        gCTTCGCCGTGCGCAATTCGATGCGTTATGCGGTGGATTATGCGGCAATGAACGACCACAGGTTGTGGCTCAAGGGATTCGCCTCGGGCGTTTCACGGCTAAACAGCCACAGAGTTATTGTTTGCTGCGTATTAcgccgc                                                                                                            <  2:921960/140‑1 (MQ=255)

                         cTTCGCCGTGCGCAATTCGATGCGTTATGCGGTGGATTATGCGGCAATGAACGACCACAGGTTGTGGCTCAAGGGATTCGCCTCGGGCGTTTCACGGTTAAACAGCCACAGAGTTATTGTTTGCTGCGTATTACgccgcc                                                                                                           >  1:457566/1‑140 (MQ=255)

                               cGTGCGCAATTCGATGCGTTATGCGGTGGATTATGCGGCAATGAACGACCACAGGTTGTGGCTCAAGGGATTCGCCTCGGGCGTTTCACGGTTAAACAGCCACAGAGTTATTGTTTGCTGCGTATTACGCCGCCTGCGCa                                                                                                     >  1:29245/1‑140 (MQ=255)

                                gTGCGCAATTCGATGCGTTATGCGGTGGATTATGCGGCAATGAACGACCACAGGTTGTGGCTCAAGGGATTCGCCTCGGGCGTTTCACGGTTAAACAGCCACAGAGTTATTGTTTGCTGCGTATTACGCCGCCTGCGCAt                                                                                                    >  1:447746/1‑140 (MQ=255)

                                gTGCGCAATTCGATGCGTTATGCGGTGGATTATGCGGCAATGAACGACCACAGGTTGTGGCTCAAGGGATTCGCCTCGGGCGTTTCACGGTTAAACAGCCACAGAGTTATTGTTTGCTGCGTATTACGCCGCCTGCGCAt                                                                                                    >  1:488594/1‑140 (MQ=255)

                                gTGCGCAATTCGATGCGTTATGCGGTGGATTATGCGGCAATGAACGACCACAGGTTGTGGCTCAAGGGATTCGCCTCGGGCGTTTCACGGTTAAACAGCCACAGAGTTATTGTTTGCTGCGTATTACGCCGCCTGCGCAt                                                                                                    >  2:84043/1‑140 (MQ=255)

                                 tGCGCAATTCGATGCGTTATGCGGTGGATTATGCGGCAATGAACGACCACAGGTTGTGGCTCAAGGGATTCGCCTCGGGCGTTTCACGGTTAAACAGCCACAGAGTTATTGTTTGCTGCGTATTACGCCGCCTGCGCATc                                                                                                   <  1:551345/140‑1 (MQ=255)

                                 tGCGCAATTCGATGCGTTATGCGGTGGATTATGCGGCAATGAACGACCACAGGTTGTGGCTCAAGGGATTCGCCTCGGGCGTTTCACGGTTAAACAGCCACAGAGTTATTGTTTGCTGCGTATTACGCCGCCTGCGCATc                                                                                                   >  1:1127929/1‑140 (MQ=255)

                                   cgcAATTCGATGCGTTATGCGGTGGATTATGCGGCAATGAACGACCACAGGTTGTGGCTCAAGGGATTCGCCTCGGGCGTTTCACGGTTAAACAGCCACAGAGTTATTGTTTGCTGCGTATTACGCCGCCTGCGCATCCt                                                                                                 >  1:295731/1‑140 (MQ=255)

                                      aaTTCGATGCGTTATGCGGTGGATTATGCGGCAATGAACGACCACAGGTTGTGGCTCAAGGGATTCGCCTCGGGCGTTTCACGGTTAAACAGCCACAGAGTTATTGTTTGCTGCGTATTACGCCGCCTGCGCATCCTCaa                                                                                              >  2:845008/1‑140 (MQ=255)

                                                gTTATGCGGTGGATTATGCGGCAATGAACGACCACAGGTTGTGGCTCAAGGGATTCGCCTCGGGCGTTTCACGGTTAAACAGCCACAGAGTTATTGTTTGCTGCGTATTGCGCCGCCTGCGCATCCTCAACCACTTGCAg                                                                                    <  1:200763/140‑1 (MQ=255)

                                                     gCGGTGGATTATGCGGCAATGAACGACCACAGGTTGTGGCTCAAGGGATTCGCCTCGGGCGTTTCACGGTTAAACAGCCACAGAGTTATTGTTTGCTGCGTATTACGCCGCCTGCGCATCCTCAACCACTTGCAGCTGCa                                                                               <  1:273411/140‑1 (MQ=255)

                                                          ggATTATGCGGCAATGAACGACCACAGGTTGTGGCTCAAGGGATTCGCCTCGGGCGTTTCACGGTTAAACAGCCACAGAGTTATTGTTTGCTGCGTATTACGCCGCCTGCGCATCCTCAACCACTTGCAGCTGCATGGAt                                                                          >  1:232959/1‑140 (MQ=255)

                                                           gATTATGCGGCAATGAACGACCACAGGTTGTGGCTCAAGGGATTCGCCTCGGGCGTTTCACGGTTAAACAGCCACAGAGTTATTGTTTGCTGCGTATTACGCCGCCTGCGCATCCTCAACCACTTGCAGCTGCATGGATg                                                                         >  2:746251/1‑140 (MQ=255)

                                                           gATTATGCGGCAATGAACGACCACAGGTTGTGGCTCAAGGGATTCGCCTCGGGCGTTTCACGGTTAAACAGCCACAGAGTTATTGTTTGCTGCGTATTACGCCGCCTGCGCATCCTCAACCACTTGCAGCTGCATGGATg                                                                         >  1:1177443/1‑140 (MQ=255)

                                                            aTTATGCGGCAATGAACGACCACAGGTTGTGGCTCAAGGGATTCGCCTCGGGCGTTTCACGGTTAAACAGCCACAGAGTTATTGTTTGCTGCGTATTACGCC‑CCTGCGCATCCTCAACCACTTGCAGCTGCATGGATGCa                                                                       <  1:1055497/140‑1 (MQ=255)

                                                                tGCGGCAATGAACGACCACAGGTTGTGGCTCAAGGGATTCGCCTCGGGCGTTTCACGGTTAAACAGCCACAGAGTTATTGTTTGCTGCGTATTACGCCGCCTGCGCATCCTCAACCACTTGCAACTGCCTGGATGCAAcg                                                                    >  1:629046/1‑140 (MQ=255)

                                                                tGCGGCAATGAACGACCACAGGTTGTAGCTCAAGGGATTCGCCTCGGGCGTTTCACGGTTAAACAGCCACAGAGTTATTGTTTGCTGCGTATTACGCCGCCTGCGCATCCTCAACCACTTGCAGCTGCATGGATGCAAcg                                                                    >  2:128135/1‑140 (MQ=255)

                                                                  aggCAATGAACGACCACAGGTTGTGGCTCAAGGGATTCGCCTCGGGCGTTTCACGGTTAAACAGCCACAGAGTTATTGTTTGCTGCGTATTACGCCGCCTGCGCATCCTCAACCACTTGCAGCTGCATGGATGCAAcgcg                                                                  <  2:232959/139‑1 (MQ=255)

                                                                   ggCAATGAACGACCACAGGTTGTGGCTCAAGGGATTCGCCTCGGGCGTTTCACGGTTAAACAGCCACAGAGTTATTGTTTGCTGCGTATTACGCCGCCTGCGCATCCTCAACCACTTGCAGCTGCATGGATGCAAcgcgc                                                                 >  1:670665/1‑140 (MQ=255)

                                                                     cAATGAACGACCACAGGTTGTGGCTCAAGGGATTCGCCTCGGGCGTTTCACGGTTAAACAGCCACAGAGTTATTGTTTGCTGCGTATTACGCCGCCTGCGCATCCTCAACCACTTGCAGCTGCATGGATGCAACGCGCgg                                                               >  2:1099707/1‑140 (MQ=255)

                                                                      aaTGAACGACCACAGGTTGTGGCTCAAGGGATTCGCCTCGGGCGTTTCACGGTTAAACAGCCACAGAGTTATTGTTTGCTGCGTATTACGCCGCCTGCGCATCCTCAACCACTTGCAGCTGCATGGATGCAACGCGCgga                                                              <  1:739059/140‑1 (MQ=255)

                                                                       aTGAACGACCACAGGTTGTGGCTCAAGGGATTCGCCTCGGGCGTTTCACGGTTAAACAGCCACAGAGTTATTGTTTGCTGCGTATTACGCCGCCTGCGCATCCTCAACCACTTGCAGCTGCATGGATGCAACGCGCggag                                                             <  2:29245/140‑1 (MQ=255)

                                                                       aTGAACGACCACAGGTTGTGGCTCAAGGGATTCGCCTCGGGCGTTTCACGGTTAAACAGCCACAGAGTTATTGTTTGCTGCGTATTACGCCGCCTGCGCATCCTCAACCACTTGCAGCTGCATGGATGCAACGCGCggag                                                             >  2:287124/1‑140 (MQ=255)

                                                                          aaCGACCACAGGTTGTGGCTCAAGGGATTCGCCTCGGGCGTTTCACGGTTAAACAGCCACAGAGTTATTGTTTGCTGCGTATTACGCCGCCTGCGCATCCTCAACCACTTGCAGCTGCATGGATGCAACGCGCGGAGGaa                                                          >  2:876399/1‑140 (MQ=255)

                                                                                cacaGGTTGTGGCTCAAGGGATTCGCCTCGGGCGTTTCACGGTTAAACAGCCACAGAGTTATTGTTTGCTGCGTATTACGCCGCCTGCGCATCCTCAACCACTTGCAGCTGCATGGATGCAACGCGCGGAGGAAACCggg                                                    <  2:81636/140‑1 (MQ=255)

                                                                                  caGGTTGTGGCTCAAGGGATTCGCCTCGGGCGTTTCACGGTTAAACAGCCACAGAGTTATTGTTTGCTGCGTATTACGCCGCCTGCGCATCCTCAACCACTTGCAGCTGCATGGATGCAACGCGCGGAGGAAACCGGGCt                                                  <  2:447746/140‑1 (MQ=255)

                                                                                  aaGGTTGTGGCTCAAGGGATTCGCCTCGGGCGTTTCACGGTTAAACAGCCACAGAGTTATTGTTTGCTGCGTATTACGCCGCCTGCGCATCCTCAACCACTTGCAGCTGCATGGATGCAACGCGCGGAGGAAACCGGGCt                                                  <  2:467794/139‑1 (MQ=255)

                                                                                   aGGTTGTGGCTCAAGGGATTCGCCTCGGGCGTTTCACGGTTAAACAGCCACAGAGTTATTGTTTGCTGCGTATTACGCCGCCTGCGCATCCTCAACCACTTGCAGCTGCATGGATGCAACGCGCGGAGGAAACCGGGCtt                                                 <  2:810583/140‑1 (MQ=255)

                                                                                    ggTTGTGGCTCAAGGGATTCGCCTCGGGCGTTTCACGGTTAAACAGCCACAGAGTTATTGTTTGCTGCGTATTACGCCGCCTGCGCATCCTCAACCACTTGCAGCTGCATGGATGCAACGCGCGGAGGAAACCGGGCttt                                                >  1:827633/1‑140 (MQ=255)

                                                                                       tgtgGCTCAAGGGATTCGCCTCGGGCGTTTCACGGTTAAACAGCCACAGAGTTATTGTTTGCTGCGTATTACGCCGCCTGCGCATCCTCAACCGCTTGCAGCTGCATGGATGCAACGCGCGGAGGAAACCGGGCTTTTCg                                             <  1:1232096/140‑1 (MQ=255)

                                                                                       tgtgGCTCAAGGGATTCGCCTCGGGCGTTTCACGGTTAAACAGCCACAGAGTTATTGTTTGCTGCGTATTACGCCGCCTGCGCATCCTCAACCACTTGCAGCTGCATGGATGCAACGCGCGGAGGAAACCGGGCTTTTCg                                             <  2:117504/140‑1 (MQ=255)

                                                                                         tgGCTCAAGGGATTCGCCTCCGGCGTTTCACGGTTAAACAGCCACTGAGTTATTGTTTGCTGCGTATTACGCCGCCTGCGCATCCTCAACCACTTGCAGCTGCATGGATGCAACGCGCGGAGGAAACCGAGCTTTTCggt                                           >  1:414653/1‑139 (MQ=255)

                                                                                               aaGGGATTCGCCTCGGGCGTTTCACGGTTAAACAGCCACAGAGTTATTGTTTGCTGCGTATTACGCCGCCTGCGCATCCTCAACCACTTGCAGCTGCATGGATGCAACGCGCGGAGGAAACCGGGCTTTTCGGGCCACTg                                     >  1:189103/1‑140 (MQ=255)

                                                                                                 gggATTCGCCTCGGGCGTTTCACGGTTAAACAGCCACAGAGTTATTGTTTGCTGCGTATTACGCCGCCTGCGCATCCTCAACCACTTGCAGCTGCATGGATGCAACGCGCGGAGGAAACCGGGCTTTTCGGGCCACTGGc                                   >  1:1232425/1‑140 (MQ=255)

                                                                                                     ttCGCCTCGGGCGTTTCACGGTTAAACAGCCACAGAGTTATTGTTTGCTGCGTATTACGCCGCCTGCGCATCCTCAACCACTTGCAGCTGCATGGATGCAACGCGCGGAGGAAACCGGGCTTTTCGGGCCACTGGCGTTg                               >  2:369242/1‑140 (MQ=255)

                                                                                                     ttCGCCTCGGGCGTTTCACGGTTAAACAGCCACAGAGTTATTGTTTGCTGCGTATTACGCCGCCTGCGCATCCTCAACCACTTGCAGCTGCATGGATGCAACGCGCGGAGGAAACCGGGCTTTTCGGGCCACTGGCGTTg                               >  2:1231306/1‑140 (MQ=255)

                                                                                                      tCGCCTCGGGCGTTTCACGGTTAAACAGCCACAGAGTTATTGTTTGCTGCGTATTACGCCGCCTGCGCATCCTCAACCACTTGCAGCTGCATGGATGCAACGCGCGGAGGAAACCGGGCTTTTCGGGCCACTGGCGTTgg                              >  2:975536/1‑140 (MQ=255)

                                                                                                      tCGCCTCGGGCGTTTCACGGTTAAACAGCCACAGAGTTATTGTTTGCTGCGTATTACGCCGCCTGCGCATCCTCAACCACTTGCAGCTGCATGGATGCAACGCGCGGAGGAAACCGGGCTTTTCGGGCCACTGGCGTTgg                              >  2:1284565/1‑140 (MQ=255)

                                                                                                      tCGCCTCGGGCGTTTCACGGTTAAACAGCCACAGAGTTATTGTTTGCTGCGTATTACGCCGCCTGCGCATCCTCAACCACTTGCAGCTGCATGGATGCAACGCGCGGAGGAAACCGGGCTTTTCGGGCCACTGGCGTTgg                              >  1:409854/1‑140 (MQ=255)

                                                                                                        gCCTCGGGCGTTTCACGGTTAAACAGCCACAGAGTTATTGTTTGCTGCGTATTACGCCGCCTGCGCATCCTCAACCACTTGCAGCTGCATGGATGCAACGCGCGGAGGAAACCGGGCTTTTCGGGCCACTGGCGTTggcg                            <  2:543157/140‑1 (MQ=255)

                                                                                                               gCGTTTCACGGTTAAACAGCCACAGAGTTATTGTTTGCTGCGTATTACGCCGCCTGCGCATCCTCAACCACTTGCAGCTGCATGGATGCAACGCGCGGAGGAAACCGGGCTTTTCGGGCCACTGGCGTTGGCGGCAAGCg                     <  2:295731/140‑1 (MQ=255)

                                                                                                                    tCACGGTTAAACAGCCACAGAGTTATTGTTTGCTGCGTATTACGCCGCCTGCGCATCCTCAACCACTTGCAGCTGCATGGATGCAACGCGCGGAGGAAACCGGGCTTTTCGGGCCACTGGCGTTGGCGGCAAGCGATCCg                >  1:688646/1‑140 (MQ=255)

                                                                                                                     cACGGTTAAACAGCCACAGAGTTATTGTTTGCTGCGTATTACGCCGCCTGCGCATCCTCAACCACTTGCAGCTGCATGGATGCAACGCGCGGAGGAAACCGGGCTTTTCGGGCCACTGGCGTTGGCGGCAAGCGATCCGc               <  2:670665/140‑1 (MQ=255)

                                                                                                                      aCGGTTAAACAGCCACAGAGTTATTGTTTGCTGCGTATTACGCCGCCTGCGCATCCTCAACCACTTGCAGCTGCATGGATGCAACGCGCGGAGGAAACCGGGCTTTTCGGGCCACTGGCGTTGGCGGCAAGCGATCCGct              <  2:823112/140‑1 (MQ=255)

                                                                                                                      aCGGTTAAACAGCCACAGAGTTATTGTTTGCTGCGTATTACGCCGCCTGCGCATCCTCAACCACTTGCAGCTGCATGGATGCAACGCGCGGAGGAAACCGGGCTTTTCGGGCCACTGGCGTTGGCGGCAAGCGATCCGct              <  2:827633/140‑1 (MQ=255)

                                                                                                                           tAAACAGCCACAGAGTTATTGTTTGCTGCGTATTACGCCGCCTGCGCATCCTCAACCACTTGCAGCTGCATGGATGCAACGCGCGGAGGAAACCGGGCTTTTCGGGCCACTGGCGTTGGCGGCAAGCGATCCGctgcctg         <  1:1191343/140‑1 (MQ=255)

                                                                                                                             aaCAGCCACAGAGTTATTGTTTGCTGCGTATTACGCCGCCTGCGCATCCTCAACCACTTGCAGCTGCATGGATGCAACGCGCGGAGGAAACCGGGCTTTTCGGGCCACTGGCGTTGGCGGCAAGCGATCCGCTGCctgct       >  1:1265782/1‑140 (MQ=255)

                                                                                                                                  ccACAGAGTTATTGTTTGCTGCGTATTACGCCGCCTGCGCATCCTCAACCACTTGCAGCTGCATGGATGCAACGCGCGGAGGAAACCGGGCTTTTCGGGCCACTGGCGTTGGCGGCAAGCGATCCGCTGCCTGCTGAGtt  >  2:1086023/1‑140 (MQ=255)

TGGTTTCGGCCCCGGAGGATGGGTGCTTCGCCGTGCGCAATTCGATGCGTTATGCGGTGGATTATGCGGCAATGAACGACCACAGGTTGTGGCTCAAGGGATTCGCCTCGGGCGTTTCACGGTTAAACAGCCACAGCGTTATTGTTTGCTGCGTATTACGCCGCCTGCGCATCCTCAACCACTTGCAGCTGCATGGATGCAACGCGCGGAGGAAACCGGGCTTTTCGGGCCACTGGCGTTGGCGGCAAGCGATCCGCTGCCTGCTGAGTT  >  NC_000913/549767‑550036

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A