Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A18 F48 I1 R1	70	76.3	2572620	98.7%	2539175	139.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	563,542	C→A	V71V (GTC→GTA)	sfmF →	putative fimbrial protein SfmF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	563,542	0	C	A	98.9%	323.4 / ‑3.0	94	V71V (GTC→GTA)	sfmF	putative fimbrial protein SfmF
Reads supporting (aligned to +/- strand): ref base C (1/0); new base A (48/45); total (49/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.29e-01

ACGATTAATATCAATTTGCACGGTAACGTTGTTGATTTCTCCTGTACCGTAAACACAGCGGATATTGATAAGACGGTAGATTTAGGCAGATGGCCTACGACACAACTACTGAACGCTGGCGATACCACGGCACTCGTCCCTTTTAGCCTGCGGCTGGAGGGATGTCCTCCGGGTTCAGTTGCGATTTTATTTACGGGAACGCCGGCATCCGATACCAACCTGCTGGCTCTGGATGATCCCGCAATGGCACAAACCGTCGCCATCGAAT  >  NC_000913/563405‑563672

aCGATTAATATCAATTTGCACGGTAACGTTGTTGATTTCTCCTGTACCGTAAACACAGCGGATATTGATAAGACGGTAGATTTAGGCAGATGGCCTACGACACAACTACTGAACGCTGGCGATACCACGGCACTCGTacc                                                                                                                                  >  1:746010/1‑140 (MQ=255)

   aTTAATATCAATTTGCACGGTAACGTTGTTGATTTCTCCTGTACCGTAAACACAGCGGATATTGATAAGACGGTAGATTTAGGCAGATGGTCTACGACACAACTACTGAACGCTGGCGATACCACGGCACTCGTACCttt                                                                                                                               >  2:975447/1‑140 (MQ=255)

           cAATTTGGATGGTAACGTTGTTGATTTCTTCTGTACCGTAAACACAGCGGATATTGATGAGACGGTAGATTTAGGCACATGGCCTACGACACAACTCCTGAACGCTGGCGATACCACGGCACTCGTACCTTTTAGCCTGc                                                                                                                       <  2:1250574/140‑1 (MQ=255)

           cAATTTGCACGGTAACGTTGTTGATTTCTCCTGTACCGTAAACACAGCGGATATTGATAAGACGGTAGATTTAGGCAGATGGCCTACGACACAACTACTGAACGCTGGCGATACCACGGCACTCGTACCTTTTAGCCTGc                                                                                                                       <  2:1133899/140‑1 (MQ=255)

            aaTTTGCACGGTAACGTTGTTGATTTCTCCTGTACCGTAAACACAGCGGATATTGATAAGACGGTATATTTAGGCAGATGGCCTACTACACAACTACTGAACGCTGGCGTTACCACGGCACTCGTACCTTTTAGCCTGCg                                                                                                                      >  1:311967/1‑140 (MQ=255)

             aTTTGCACGGTAACGTTGTTGATTTCTCCTGTACCGTAAACACAGCGGATATTGATAAGACGGTAGATTTAGGCAGATGGCCTACGACACAACTACTGAACGCTGGCGATACCACGGCACTCGTACCTTTTAGCCTGCgg                                                                                                                     >  1:526542/1‑140 (MQ=255)

              tttGCACGGTAACGTTGTTGATTTCTCCTGTACCGTAAACACAGCGGATATTGATAAGACGGAAGATTTAGGCAGATGGCCTACGACACAACTACTGAACGCTGGCGATACCACGGCACTCGTACCTTTTAGCCTGCGGc                                                                                                                    >  2:966577/1‑140 (MQ=255)

                tGCACGGTAACGTTGTTGATTTCTCCTGTACCGTAAACACAGCGGATATTGATAAGACGGTAGATTTAGGCAGATGGCCTACGACACAACTACTGAACGCTGGCGATACCACGGCACTCGTACCTTTTAGCCTGCGGCTg                                                                                                                  <  1:1123822/140‑1 (MQ=255)

                tGCACGGTAACGTTGTTGATTTCTCCTGTACCGTAAACACAGCGGATATTGATAAGACGGTAGATTTAGGCAGATGGCCTACGACACAACTACTGAACGCTGGCGATACCACGGCACTCGTACCTTTTAGCCTGCGGCTg                                                                                                                  <  1:893270/140‑1 (MQ=255)

                      gTAACGTTGTTGATTTCTCCTGTACCGTAAACACAGCGGATATTGATAAGACGGTAGATTTAGGCAGATGGCCTACGACACAACTACTGAACGCTGGCGATACCACGGCACTCGTACCTTTTAGCCTGCGGCTGGAGGGa                                                                                                            <  2:1219180/140‑1 (MQ=255)

                      gTAACGTTGTTGATTTCTCCTGTACCGTAAACACAGCGGATATTGATAAGACGGTAGATTTAGGCAGATGGCCTACGACACAACTACTGAACGCTGGCGATACCACGGCACTCGTACCTTTTAGCCTGCGGCTGGAGGGa                                                                                                            >  1:1187881/1‑140 (MQ=255)

                      gTAACGTTGTTGATTTCTCCTGTACCGTAAACACAGCGGATATTGATAAGACGGTAGATTTAGGCAGATGGCCTACGACACAACTACTGAACGCTGGCGATACCACGGCACTCGTACCTTTTAGCCTGCGGCTGGAGGGa                                                                                                            <  1:1272477/140‑1 (MQ=255)

                       tAACGTTGTTGATTTCTCCTGTACCGTAAACACAGCGGATATTGATAAGACGGTAGATTTAGGCAGATGGCCTACGACACAACTACTGAACGCTGGCGATACCACGGCACTCGTACCTTTTAGCCTGCGGCTGGAGGGAt                                                                                                           <  2:273556/140‑1 (MQ=255)

                       tAACGTTGTTGATTTCTCCTGTACCGTAAACACAGCGGATATTGATAAGACGGTAGATTTAGGCAGATGGCCTACGACACAACTACTGAACGCTGGCGATACCACGGCACTCGTACCTTTTAGCCTGCGGCTGGAGGGAt                                                                                                           >  2:372971/1‑140 (MQ=255)

                       tAACGTTGTTGATTTCTCCTGTACCGTAAACACAGCGGATATTGATAAGACGGTAGATTTAGGCAGATGGCCTACGACACAACTACTGAACGCTGGCGATACCACGGCACTCGTACCTTTTAGCCTGCGGCTGGAGGGAt                                                                                                           <  1:457654/140‑1 (MQ=255)

                        aaCGTTGTTGATTTCTCCTGTACCGTAAACACAGCGGATATTGATAAGACGGTAGATTTAGGCAGATGGCCTACGACACAACTACTGAACGCTGGCGATACCACGGCACTCGTACCTTTTAGCCTGCGGCTGGAGGGATg                                                                                                          >  1:628386/1‑140 (MQ=255)

                            ttgttgATTTCTCCTGTACCGTAAACACAGCGGATATTGATAAGACGGTAGATTTAGGCAGATGGCCTACGACACAACTACTGAACGCTGGCGATACCACGGCACTCGTACCTTTTAGCCTGCGGCTGGAGGGATGtcct                                                                                                      >  2:943140/1‑140 (MQ=255)

                               ttgATTTCTCCTGTACCGTAAACACAGCGGATATTGATAAGACGGTAGATTTAGGCAGATGGCCTACGACACAACTACTGAACGCTGGCGATACCACGGCACTCGTACCTTTTAGCCTGCGGCTGGAGGGATGTCCTCCg                                                                                                   >  1:1159084/1‑140 (MQ=255)

                               ttgATTTCTCCTGTACCGTAAACACAGCGGATATTGAAAAGACGGTAGATTTAGGCATATGGCCGGCGACACAACTACTGAACGCTGGCGATACCACGGCACTCGTACCTTTTAGCCTGCGGCTGGAGGGATGTCCTCCg                                                                                                   >  2:858001/1‑140 (MQ=255)

                                 gATTTCTCCTGTACCGTAAACACAGCGGATATTGATAAGACGGTAGATTTAGGCAGATGGCCTACGACACAACTACTGAACGCTGGCGATACCACGGCACTCGTACCTTTTAGCCTGCGGCTGGAGGGATGTCCTCCggg                                                                                                 <  2:753468/140‑1 (MQ=255)

                                 gATTTCTCCTGTACCGTAAACACAGCGGATATTGATAAGACGGTAGATTTAGGCAGATGGCCTACGACACAACTACTGAACGCTGGCGATACCACGGCACTCGTACCTTTTAGCCTGCGGCTGGAGGGATGTCCTCCggg                                                                                                 >  2:771651/1‑140 (MQ=255)

                                 gATTTCTCCTGTACCGTAAACACAGCGGATATTGATAAGACGGTAGATTTAGGCAGATGGCCTACGACACAACTACTGAACGCTGGCGATACCACGGCACTCGTACCTTTTAGCCTGCGGCTGGAGGGATGTCCTCCggg                                                                                                 >  1:378082/1‑140 (MQ=255)

                                     tctcCTGTACCGTAAACACAGCGGATATTGATAAGACGGTAGATTTAGGCAGATGGCCTACGACACAACTACTGAACGCTGGCGATACCACGGCACTCGTACCTTTTAGCCTGCGGCTGGAGGGATGTCCTCCGGGTTCa                                                                                             >  1:93704/1‑140 (MQ=255)

                                      ctcCTGTACCGTAAACACAGCGGATATTGATAAGACGGTAGATTTAGGCAGATGGCCTACGACACAACTACTGAACGCTGGCGATACCACGGCACTCGTACCTTTTAGCCTGCGGCTGGAGGGATGTCCTCCGGGTTCAg                                                                                            >  1:434854/1‑140 (MQ=255)

                                         cTGTACCGTAAACACAGCGGATATTTATAAGACGGTAGATTTAGGCAGATGGCCTACGACACAACTACTGAACGCTGGCGATACCACGGCACTCGTACCTTTTAGCCTGCGGCTGGAGGGATGTCCTCCGGGTTCAGTTg                                                                                         <  1:1021245/140‑1 (MQ=255)

                                         cTGTACCGTAAACACAGCGGATATTGATAAGACGGTAGATTTAGGCAGATGGCCTACGACACAACTACTGAACGCTGGCGATACCACGGCACTCGTACCTTTTAGCCTGCGGCTGGAGGGATGTCCTCCGGGTTCAGTTg                                                                                         >  1:1229598/1‑140 (MQ=255)

                                           gTACCGTAAACACAGCGGATATTGATAAGACGGTAGATTTAGGCAGATGGCCTACGACACAACTACTGAACGCTGGCGATACCACGGCACTCGTACCTTTTAGCCTGCGGCTGGAGGGATGTCCTCCGGGTTCAGTTGCg                                                                                       <  2:351557/140‑1 (MQ=255)

                                            aaCCGTAAACACAGCGGATATTGATAAAACGGCAGATTTAGCCAGAGGGCCTACGACACAACTACTGACCGCTGGCGATACCACCGCACTCGTACCTTTTAGCCTGCGGCTGGAGGGATGTCCTCCGGGTTCAGTTGCGa                                                                                      >  2:268789/2‑140 (MQ=255)

                                             aCCGTAAACACAGCGGATATTGATAAGACGGTAGATTTAGGCAGATGGCCTACGACACAACTACTGAACGCTGGCGATACCACGGCACTCGTACCTTTTAGCCTGCGGCTGGAGGGATGTCCTCCGGGTTCAGTTGCGAt                                                                                     <  2:1229598/140‑1 (MQ=255)

                                              acGTAAACACAGCGGATATTGATAAGACGGTAGATTTAGGCAGATGGCCTACGACACAACTACTGAACGCTGGCGATACCACGGCACTCGTACCTTTTAGCCTGCGGCTGGAGGGATGTCCTCCGGGTTCAGTTGCGAtt                                                                                    <  2:1063628/139‑1 (MQ=255)

                                                 tAAACACAGCGGATATTGATAAGACGGTAGATTTAGGCAGATGGCCTACGACACAACTACTGAACGCTGGCGATACCACGGCACTCGTACCTTTTAGCCTGCGGCTGGAGGGATGTCCTCCGGGTTCAGTTGCGAttttt                                                                                 >  1:1137007/1‑139 (MQ=255)

                                                 tAAACACAGCGGATATTGATAAGACGGTAGATTTAGGCAGATGGCCTACGACACAACTACTGAACGCTGGCGATACCACGGCACTCGTACCTTTTAGCCTGCGGCTGGAGGGATGTCCTCCGGGTTCAGTTGCGATttta                                                                                 >  1:1053255/1‑140 (MQ=255)

                                                 tAAACACAGCGGATATTGATAAGACGGTAGATTTAGGCAGATGGCCTACGACACAACTACTGAACGCTGGCGATACCACGGCACTCGTACCTTTTAGCCTGCGGCTGGAGGGATGTCCTCCGGGTTCAGTTGCGATttta                                                                                 >  1:772205/1‑140 (MQ=255)

                                                        aGCGGATATTGATAAGACGGTAGATTTAGGCAGATGGCCTACGACACAACTACTGAACGCTGGCGATACCACGGCACTCGTACCTTTTAGCCTGCGGCTGGAGGGATGTCCTCCGGGTTCAGTTGCGATTTTATTTACgg                                                                          >  2:621630/1‑140 (MQ=255)

                                                        aGCGGATATTGATAAGACGGTAGATTTAGGCACATAGCCTACGACACAACTACTGAACGCTGGGGATACCACGGCACTCGTACCTTTTAGCCTGCGGCTGGAGGGATGTCCTCCGGGTTCAGTTGCGATTTTATTTACgt                                                                          >  2:311098/1‑139 (MQ=255)

                                                         gCGGATATTGATAAGACGGTAGATTTAGGCAGATGGCCTACGACACAACTACTGAACGCTGGCGATACCACGGCACTCGTCCCTTTTAGCCTGCGGCTGGAGGGATGTCCTCCGGGTTCAGTTGCGATTTTATTTACggg                                                                         >  1:1140649/1‑140 (MQ=255)

                                                         gCGGATATTGATAAGACGGTAGATTTAGGCAGATGGCCTACGACACAACTACTGAACGCTGGCGATACCACGGCACTCGTACCTTTTAGCCTGCGGCTGGAGGGATGTCCTCCGGGTTCAGTTGCGATTTTATTTACggg                                                                         >  1:987041/1‑140 (MQ=255)

                                                         gCGGATATTGATAAGACGGTAGATTTAGGCAGATGGCCTACGACACAACTACTGAACGCTGGCGATACCACGGCACTCGTACCTTTTAGCCTGCGGCTGGAGGGATGTCCTCCGGGTTCAGTTGCGATTTTATTTACggg                                                                         >  2:113159/1‑140 (MQ=255)

                                                         gCGGATATTGATAAGACGGTAGATTTAGGCAGATGGCCTACGACACAACTACTGAACGCTGGCGATACCACGGCACTCGTACCTTTTAGCCTGCGGCTGGAGGGATGTCCTCCGGGTTCAGTTGCGATTTTATTTACggg                                                                         >  1:316572/1‑140 (MQ=255)

                                                          cGGATATTGATAAGACGGTAGATTTAGGCAGATGGCCTACGACACAACTACTGAACGCTGGCGATACCACGGCACTCGTACCTTTTAGCCTGCGGCTGGAGGGATGTCCTCCGGGTTCAGTTGCGATTTTATTTACGGGa                                                                        >  2:180221/1‑140 (MQ=255)

                                                           ggATATTGATAAGACGGTAGATTTAGGCAGATGGCCTACGACACAACTACTGAACGCTGGCGATACCACGGCACTCGTACCTTTTAGCCTGCGGCTGGAGGGATGTCCTCCGGGTTCAGTTGCGATTTTATTTACGGGaa                                                                       >  2:1278303/1‑140 (MQ=255)

                                                           ggATATTGATAAGACGGTAGATTTAGGCAGATGGCCTACGACACAACTACTGAACGCTGGCGATACCACGGCACTCGTACCTTTTAGCCTGCGGCTGGAGGGATGTCCTCCGGGTTCAGTTGCGATTTTATTTACGGGaa                                                                       >  2:694196/1‑140 (MQ=255)

                                                                 tGATAAGACGGTAGATTTAGGCAGATGGCCTACGACACAACTACTGAACGCTGGCGATACCACGGCACTCGTACCTTTTAGCCTGCGGCTGGAGGGATGTCCTCCGGGTTCAGTTGCGATTTTATTTACGGGAACGCCgg                                                                 <  2:628386/140‑1 (MQ=255)

                                                                     aaGACGGTAGATTTAGGCAGATGGCCTACGACACAACTACTGAACGCTGGCGATACCACGGCACTCGTACCTTTTAGCCTGCGGCTGGAGGGATGTCCTCCGGGTTCAGTTGCGATTTTATTTACGGGAACGCCGGCATc                                                             <  2:772205/140‑1 (MQ=255)

                                                                     aaGACGGTAGATTTAGGCAGATGGCCTACGACACAACTACTGAACGCTGGCGATACCACGGCACTCGTACCTTTTAGCCTGCGGCTGGAGGGATGTCCTCCGGGTTCAGTTGCGATTTTATTTACGGGAACGCCGGCATc                                                             <  2:1187881/140‑1 (MQ=255)

                                                                         cGGTAGATTTAGGCAGATGGCCTACGACACAACTACTGAACGCTGGCGATACCACGGCACTCGTACCTTTTAGCCTGCGGCTGGAGGGATGTCCTCCGGGTTCAGTTGCGATTTTATTTACGGGAACGCCGGCATCCGAt                                                         >  2:28948/1‑140 (MQ=255)

                                                                            tAGATTTAGGCAGATGGCCTACGACACAACTACTGAACGCTGGCGATACCACGGCACTCGTACCTTTTAGCCTGCGGCTGGAGGGATGTCCTCCGGGTTCAGTTGCGATTTTATTTACGGGAACGCCGGCATCCGATAcc                                                      <  2:951943/140‑1 (MQ=255)

                                                                            tAGATTTAGGCAGATGGCCTACGACACAACTACTGAACGCTGGCGATACCACGGCACTCGTACCTTTTAGCCTGCGGCTGGAGGGATGTCCTCCGGGTTCAGTTGCGATTTTATTTACGGGAACGCCGGCATCCGATAcc                                                      <  1:977667/140‑1 (MQ=255)

                                                                             aGATTTAGGCAGATGGCCTACGACACAACTACTGAACGCTGGCGATACCACGGCACTCGTACCTTTTAGCCTGCGGCTGGAGGGATGTCCTCCGGGTTCAGTTGCGATTTTATTTACGGGAACGCCGGCATCCGATACCa                                                     >  2:972168/1‑140 (MQ=255)

                                                                              gATTTAGGCAGATGGCCTACGACACAACTACTGAACGCTGGCGATACCACGGCACTCGTACCTTTTAGCCTGCGGATGGAGGGATGTCCTCCTGGTTCAGTTGCGATTTTATTTACGCGAACGCCGGCATCCGATACCaa                                                    <  1:311098/140‑1 (MQ=255)

                                                                                  tAGGCAGATGGCCTACGACACAACTACTGAACGCTGGCGATACCACGGCACTCGTACCTTTTAGCCTGCGGCTGGAGGGATGTCCTCCGGGTTCAGTTGCGATTTTATTTACGGGAACGCCGGCATCCGATACCAACctg                                                <  1:1012964/140‑1 (MQ=255)

                                                                                  tAGGCAGATGGCCTACGACACAACTACTGAACGCTGGCGATACCACGGCACTCGTACCTTTTAGCCTGCGGCTGGAGGGATGTCCTCCGGGTTCAGTTGCGATTTTATTTACGGGAACGCCGGCATCCGATACCAACctg                                                <  2:746010/140‑1 (MQ=255)

                                                                                  tAGGCAGATGGCCTACGACACAACTACTGAACGCTGGCGATACCACGGCACTCGTACCTTTTAGCCTGCGGCTGGAGGGATGTCCTCCGGGTTCAGTTGCGATTTTATTTACGGGAACGCCGGCATCCGATACCAACctg                                                <  1:626762/140‑1 (MQ=255)

                                                                                  tAGGCAGATGGCCTACGACACAACTACTGAACGCTGGCGATACCACGGCACTCGTACCTTTTAGCCTGCGGCTGGAGGGATGTCCTCCGGGTTCAGTTGCGATTTTATTTACGGGAACGCCGGCATCCGATACCAACctg                                                <  1:943140/140‑1 (MQ=255)

                                                                                   aGGCAGATGGCCTACGACACAACTACTGAACGCTGGCGATACCACGGCACTCGTACCTTTTAGCCTGCGGCTGGAGGGATGTCCTCCGGGTTCAGTTGCGATTTTATTTACGAGAACGCCGGCATCCGATACCAACctgc                                               <  1:1044750/140‑1 (MQ=255)

                                                                                    ggCAGATGGCCTACGACACAACTACTGAACGCTGGCGATACCACGGCACTCGTACCTTTTAGCCTGCGGCTGGAGGGATGTCCTCCGGGTTCAGTTGCGATTTTATTTACGGGAACGCCGGCATCCGATACCAACctgct                                              <  2:774094/140‑1 (MQ=255)

                                                                                    ggCAGATGGCCTACGACACAACTACTGAACGCTGGCGATACCACGGCACTCGTACCTTTTAGCCTGCGGCTGGAGGGATGTCCTCCGGGTTCAGTTGCGATTTTATTTACGGGAACGCCGGCATCCGATACCAACctgct                                              >  1:574138/1‑140 (MQ=255)

                                                                                    ggCAGATGGCCTACGACACAACTACTGAACGCTGGCGATACCACGGCACTCGTACCTTTTAGCCTGCGGCTGGAGGGATGTCCTCCCGGTTCAGTTGCGATTTTATTTACGTGAACGCCGGCATCCGATACCAACctgct                                              >  2:1207038/1‑140 (MQ=255)

                                                                                      aaaaTGGCCTACGACACAACTACTGAACGCTGGCGATACCACGGCACTCGTACCTTTTAGCCTGCGGCTGGAGGGATGTCCTCCGGGTTCAGTTGCGATTTTATTTACGGGAACGCCGGCATCCGATACCAACCTGCTgg                                            <  2:295232/137‑1 (MQ=255)

                                                                                        gATGGCCTACGACACAACTACTGAACGCTGGCGATACCACGGCACTCGTACCTTTTAGCCTGCGGCTGGAGGGATGTCCTCCGGGTTCAGTTGCGATTTTATTTACGGGAACGCCGGCATCCGATACCAACCTGCTGGct                                          >  2:1085826/1‑140 (MQ=255)

                                                                                        gATGGCCTACGACACAACTACTGAACGCTGGCGATACCACGGCACTCGTACCTTTTAGCCTGCGGCTGGAGGGATGTCCTCCGGGTTCAGTTGCGATTTTATTTACGGGAACGCCGGCATCCGATACCAACCTGCTGGct                                          <  1:180221/140‑1 (MQ=255)

                                                                                        gATGGCCTACGACACAACTACTGAACGCTGGCGATACCACGGCACTCGTACCTTTTAGCCTGCGGCTGGAGGGATGTCCTCCGGGTTCAGTTGCGATTTTATTTACGGGAACGCCGGCATCCGATACCAACCTGCTGGct                                          <  2:1217244/140‑1 (MQ=255)

                                                                                        gATGGCCTACGACACAACTACTGAACGCTGGCGATACCACGGCACTCGTACCTTTTAGCCTGCGGCTGGAGGGATGTCCTCCGGGTTCAGTTGCGATTTTATTTACGGGAACGCCGGCATCCGATACCAACCTGCTGGct                                          <  1:476335/140‑1 (MQ=255)

                                                                                        gATGGCCTACGACACAACTACTGAACGCTGGCGATACCACGGCACTCGTACCTTTTAGCCTGCGGCTGGAGGGATGTCCTCCGGGTTCAGTTGCGATTTTATTTACGGGAACGCCGGCATCCGATACCAACCTGCTGGct                                          <  1:966577/140‑1 (MQ=255)

                                                                                         aTGGCCTACGACACAACTACTGAACGCTGGCGATACCACGGCACTCGTACCTTTTAGCCTGCGGCTGGAGGGATGTCCTCCGGGTTCAGTTGCGATTTTATTTACGGGAACGCCGGCATCCGATACCAACCTGCTGGctc                                         >  2:1252124/1‑140 (MQ=255)

                                                                                         aTGGCCTACGACACAACTACTGAACGCTGGCGATACCACGGCACTCGTACCTTTTAGCCTGCGGCTGGAGGGATGTCCTCCGGGTTCAGTTGCGATTTTATTTACGGGAACGCCGGCATCCGATACCAACCTGCTGGctc                                         >  1:401999/1‑140 (MQ=255)

                                                                                         aTGGCCTACGACACAACTACTGAACGCTGGCGATACCACGGCACTCGTACCTTTTAGCCTGCGGCTGGAGGGATGTCCTCCGGGTTCAGTTGCGATTTTATTTACGGGAACGCCGGCATCCGATACCAACCTGCTGGctc                                         >  2:1096541/1‑140 (MQ=255)

                                                                                                   acacAACTACTGAACGCTGGCGATACCACGGCACTCGTACCTTTTAGCCTGCGGCTGGAGGGATGTCCTCCGGGTTCAGTTGCGATTTTATTTACGGGAACGCCGGCATCCGATACCAACCTGCTGGCTCTGGATGATcc                               <  2:1053255/140‑1 (MQ=255)

                                                                                                       aaCTACTGAACGCTGGCGATACCACGGCACTCGTACCTTTTAGCCTGCGGCTGGAGGGATGTCCTCCGGGTTCAGTTGCGATTTTATTTACGGGAACGCCGGCATCCGATACCAACCTGCTGGCTCTGGATGATCCCGCa                           >  1:1235495/1‑140 (MQ=255)

                                                                                                       aaCTACTGAACGCTGGCGATACCACGGCACTCGTACCTTTTAGCCTGCGGCTGGAGGGATGTCCTCCGGGTTCAGTTGCGATTTTATTTACGGGAACGCCGGCATCCGATACCAACCTGCTGGCTCTGGATGATCCCGCa                           >  2:78814/1‑140 (MQ=255)

                                                                                                       aaCTACTGAACGCTGGCGATACCACGGCACTCGTACCTTTTAGCCTGCGGCTGGAGGGATGTCCTCCGGGTTCAGTTGCGATTTTATTTACGGGAACGCCGGCATCCGATACCAACCTGCTGGCTCTGGATGATCCCGCa                           >  1:45282/1‑140 (MQ=255)

                                                                                                       aaCTACTGAACGCTGGCGATACCACGGCACTCGTACCTTTTAGCCTGCGGCTGGAGGGATGTCCTCCGGGTTCAGTTGCGATTTTATTTACGGGAACGCCGGCATCCGATACCAACCTGCTGGCTCTGGATGATCCCGCa                           >  1:1206207/1‑140 (MQ=255)

                                                                                                        actactGAACGCTGGCGATACCACGGCACTCGTACCTTTTAGCCTGCGGCTGGAGGGATGTCCTCCGGGTTCAGTTGCGATTTTATTTACGGGAACGCCGGCATCCGATACCAACCTGCTGGCTCTGGATGATCCCGCaa                          <  1:113159/140‑1 (MQ=255)

                                                                                                        actactGAACGCTGGCGATACCACGGCACTCGTACCTTTTAGCCTGCGGCTGGAGGGATGTCCTCCGGGTTCAGTTGCGATTTTATTTACGGGAACGCCGGCATCCGATACCAACCTGCTGGCTCTGGATGATCCCGCaa                          <  1:975447/140‑1 (MQ=255)

                                                                                                               aaCGCTGGCGATACCACGGCACTCGTACCTTTTAGCCTGCGGCTGGAGGGATGTCCTCCGGGTTCAGTTGCGATTTTATTTACGGGAACGCCGGCATCCGATACCAACCTGCTGGCTCTGGATGATCCCGCAATGGcaca                   >  1:1039370/1‑140 (MQ=255)

                                                                                                               aaCGCTGGCGATACCACGGCACTCGTACCTTTTAGCCTGCGGCTGGAGGGATGTCCTCCGGGTTCAGTTGCGATTTTATTTACGGGAACGCCGGCATCCGATACCAACCTGCTGGCTCTGGATGATCCCGCAATGGcaca                   >  1:406172/1‑140 (MQ=255)

                                                                                                               aaCGCTGGCGATACCACGGCACTCGTACCTTTTAGCCTGCGGCTGGAGGGATGTCCTCCGGGTTCAGTTGCGATTTTATTTACGGGAACGCCGGCATCCGATACCAACCTGCTGGCTCTGGATGATCCCGCAATGGcaca                   >  1:436491/1‑140 (MQ=255)

                                                                                                               aaCGCTGGCGATACCACGGCACTCGTACCTTTTAGCCTGCGGCTGGAGGGATGTCCTCCGGGTTCAGCTGCGATTTTATTTACGGGAACGCCGGCATCCGATACCAACCTGCTGGCTCTGGATGATCCCGCAATGGcaca                   >  2:673307/1‑140 (MQ=255)

                                                                                                                aCGCTGGCGATACCACGGCACTCGTACCTTTTAGCCTGCGGCTGGAGGGATGTCCTCCGGGTTCAGTTGCGATTTTATTTACGGGAACGCCGGCATCCGATACCAACCTGCTGGCTCTGGATGATCCCGCAATGGCACaa                  <  2:574138/140‑1 (MQ=255)

                                                                                                                 cGCTGGCGATACCACGGCACTCGTACCTTTTAGCCTGCGGCTGGAGGGATGTCCTCCGGGTTCAGTTGCGATTTTATTTACGGGAACGCCGGCATCCGATACCAACCTGCTGGCTCTGGATGATCCCGCAATGGCACaaa                 >  2:449086/1‑140 (MQ=255)

                                                                                                                 cGCTGGCGATACCACGGCACTCGTACCTTTTAGCCTGCGGCTGGAGGGATGTCCTCCGGGTTCAGTTGCGATTTTATTTACGGGAACGCCGGCATCCGATACCAACCTGCTGGCTCTGGATGATCCCGCAATGGCACaaa                 >  1:834487/1‑140 (MQ=255)

                                                                                                                 cGCTGGCGATACCACGGCACTCGTACCTTTTAGCCTGCGGCTGGAGGGATGTCCTCCGGGTTCAGTTGCGATTTTATTTACGGGAACGCCGGCATCCGATACCAACCTGCTGGCTCTGGATGATCCCGCAATGGCACaaa                 >  1:160712/1‑140 (MQ=255)

                                                                                                                   cTGGCGATACCACGGCACTCGTACCTTTTAGCCTGCGGCTGGAGGGATGTCCTCCGGGTTCAGTTGCGATTTTATTTACGGGAACGCCGGCATCCGATACCAACCTGCTGGCTCTGGATGATCCCGCAATGGCACAAAcc               >  1:1036407/1‑140 (MQ=255)

                                                                                                                   cTGGCGATACCACGGCACTCGTACCTTTTAGCCTGCGGCTGGAGGGATGTCCTCCGGGTTCAGTTGCGATTTTATTTACGGGAACGCCGGCATCCGATACCAACCTGCTGGCTCTGGATGATCCCGCAATGGCACAAAcc               >  1:1223466/1‑140 (MQ=255)

                                                                                                                     ggCGATACCACGGCACTCGTACCTTTTAGCCTGCGGCTGGAGGGATGTCCTCCGGGTTCAGTTGCGATTTTATTTACGGGAACGCCGGCATCCGATACCAACCTGCTGGCTCTGGATGATCCCGCAATGGCACAAACCGt             <  2:526542/140‑1 (MQ=255)

                                                                                                                          tACCACGGCACTCGTACCTTTTAGCCTGCGGCTGGAGGGATGTCCTCCGGGTTCAGTTGCGATTTTATTTACGGGAACGCCGGCATCCGATACCAACCTGCTGGCTCTGGATGATCCCGCAATGGCACAAACCGTCGCCa        <  1:449086/140‑1 (MQ=255)

                                                                                                                          tACCACGGCACTCGTACCTTTTAGCCTGCGGCTGGAGGGATGTCCTCCGGGTTCAGTTGCGATTTTATTTACGGGAACGCCGGCATCCGATACCAACCTGCTGGCTCTGGATGATCCCGCAATGGCACAAACCGTCGCCa        <  2:1159084/140‑1 (MQ=255)

                                                                                                                             cACGGCACTCGTACCTTTTAGCCTGCGGCTGGAGGGATGTCCTCCGGGTTCAGTTGCGATTTTATTTACGGGAACGCCGGCATCCGATACCAACCTGCTGGCTCTGGATGATCCCGCAATGGCACAAACCGTCGCCATCg     <  2:1218159/140‑1 (MQ=255)

                                                                                                                               cGGCACTCGTACCTTTTAGCCTGCGGCTGGAGGGATGTCCTCCGGGTTCAGTTGCGATTTTATTTACGGGAACGCCGGCATCCGATACCAACCTGCTGGCTCTGGATGATCCCGCAATGGCACAAACCGTCGCCATCGaa   <  1:621630/140‑1 (MQ=255)

                                                                                                                               cGGCACTCGTACCTTTTAGCCTGCGGCTGGAGGGATGTCCTCCGGGTTCAGTTGCGATTTTATTTACGGGAACGCCGGCATCCGATACCAACCTGCTGGCTCTGGATGATCCCGCAATGGCACAAACCGTCGCCATCGaa   <  2:719236/140‑1 (MQ=255)

                                                                                                                               cGGCACTCGTACCTTTTAGCCTGCGGCTGGAGGGATGTCCTCCGGGTTCAGTTGCGATTTTATTTACGGGAACGCCGGCATCCGATACCAACCTGCTGGCTCTGGATGATCCCGCAATGGCACAAACCGTCGCCATCGaa   <  1:78814/140‑1 (MQ=255)

                                                                                                                               cGGCACTCGTACCTTTTAGCCTGCGGCTGGAGGGATGTCCTCCGGGTTCAGTTGCGATTTTATTTACGGGAACGCCGGCATCCGATACCAACCTGCTGGCTCTGGATGATCCCGCAATGGCACAAACCGTCGCCATCGaa   <  2:93704/140‑1 (MQ=255)

                                                                                                                               cGGCACTCGTACCTTTTAGCCTGCGGCTGGAGGGATGTCCTCCGGGTTCAGTTGCGATTTTATTTACGGGAACGCCGGCATCCGATACCAACCTGCTGGCTCTGGATGATCCCGCAATGGCACAAACCGTCGCCATCGaa   <  2:378082/140‑1 (MQ=255)

                                                                                                                               cGGCACTCGTACCTTTTAGCCTGCGGCTGGAGGGATGTCCTCCGGGTTCAGTTGCGATTTTATTTACGGGAACGCCGGCATCCGATACCAACCTGCTGGCTCTGGATGATCCCGCAATGGCACAAACCGTCGCCATCGaa   <  1:858001/140‑1 (MQ=255)

                                                                                                                               cGGCACTCGTACCTTTTAGCCTGCGGCTGGAGGGATGTCCTCCGGGTTCAGTTGCGATTTTATTTACGGGAACGCCGGCATCCGATACCAACCTGCTGGCTCTGGATGATCCCGCAATGGCACAAACCGTCGCCATCGaa   <  1:972168/140‑1 (MQ=255)

                                                                                                                               aggCACTCGTACCTTTTAGCCTGCGGCTGGAGGGATGTCCTCCGGGTTCAGTTGCGATTTTATTTACGGGAACGCCGGCATCCGATACCAACCTGCTGGCTCTGGATGATCCCGCAATGGCACAAACCGTCGCCATCGaa   <  2:987041/139‑1 (MQ=255)

                                                                                                                                ggCACTCGTACCTTTTAGCCTGCGGCTGGAGGGATGTCCTCCGGGTTCAGTTGCGATTTTATTTACGGGAACGCCGGCATCCGATACCAACCTGCTGGCTCTGGATGATCCCGCAATGGCACAAACCGTCGCCATCGAAt  >  1:810466/1‑140 (MQ=255)

                                                                                                                                ggCACTCGTACCTTTTAGCCTGCGGCTGGAGGGATGTCCTCCGGGTTCAGTTGCGATTTTATTTACGGGAACGCCGGCATCCGATACCAACCTGCTGGCTCTGGATGATCCCGCAATGGCACAAACCGTCGCCATCGAAt  >  1:367514/1‑140 (MQ=255)

ACGATTAATATCAATTTGCACGGTAACGTTGTTGATTTCTCCTGTACCGTAAACACAGCGGATATTGATAAGACGGTAGATTTAGGCAGATGGCCTACGACACAACTACTGAACGCTGGCGATACCACGGCACTCGTCCCTTTTAGCCTGCGGCTGGAGGGATGTCCTCCGGGTTCAGTTGCGATTTTATTTACGGGAACGCCGGCATCCGATACCAACCTGCTGGCTCTGGATGATCCCGCAATGGCACAAACCGTCGCCATCGAAT  >  NC_000913/563405‑563672

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A