Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A17181920 F51 I0 R1 152 403.6 11685478 99.6% 11638736 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 3,724,078 C→T 100% S250N (AGT→AAT)  U712_19200 ← SacPA operon antiterminator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068813,724,0780CT100.0% 1697.9 / NA 476S250N (AGT→AAT) U712_19200SacPA operon antiterminator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (245/231);  total (245/231)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 480 total aligned reads displayed.

TATTACACGCTCAATCCCGATAAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGACTCCGGCAAATGTAATTGATATTCACTTTTCAAAAACTCTGCCAGCTCAAGCGCACATTGATAGGCAAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGACTGACTGCGTATCTCAAATGCGTGACAAGG  >  CP006881/3723931‑3724224
                                                                                                                                                   |                                                                                                                                                  
tactACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCAttt                                                                                                                                                  <  2:3958553/147‑1 (MQ=255)
     cacaCTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGTCATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGGAATTGATATTCATTTTTCaa                                                                                                                                             >  1:1010600/1‑150 (MQ=255)
      acactgaaTCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCaaa                                                                                                                                            <  1:4887511/144‑1 (MQ=255)
      acactgaaTCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCaaa                                                                                                                                            >  2:5689823/7‑150 (MQ=255)
       cactgaaTCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCaaaa                                                                                                                                           <  1:1083669/145‑1 (MQ=255)
       cactgaaTCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCaaaa                                                                                                                                           <  2:490335/145‑1 (MQ=255)
         ctgaaTCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAAc                                                                                                                                         >  2:1019158/4‑150 (MQ=255)
           gaaTCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAActc                                                                                                                                       <  1:4042615/149‑1 (MQ=255)
           gaaTCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAActc                                                                                                                                       <  1:3654351/149‑1 (MQ=255)
             aTCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAActccg                                                                                                                                     >  2:2743044/1‑148 (MQ=255)
                 tgATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAg                                                                                                                                 >  2:5490492/2‑150 (MQ=255)
                  gATCAATAAAAAAACTTCCAGAGATCAGTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCAGCCTCGGATTCCGTCAAATGTAATTGATATTCATTTTTTAAAAACTCCACCAGc                                                                                                                                >  1:5528607/1‑150 (MQ=255)
                      aataaaAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTcca                                                                                                                            >  1:3145345/1‑148 (MQ=255)
                        taaaAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAgc                                                                                                                          <  2:241164/150‑1 (MQ=255)
                        taaaAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAgc                                                                                                                          >  1:2113006/1‑150 (MQ=255)
                        taaaAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAgc                                                                                                                          <  1:2805087/150‑1 (MQ=255)
                         aaaaaaaCTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAgcg                                                                                                                         >  2:5799114/1‑150 (MQ=255)
                            aaaaCTTCCAGAGATCACTCCCTGCAAGTCAAAGCGATTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGcac                                                                                                                      <  1:3559312/150‑1 (MQ=255)
                             aaaCTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGcaca                                                                                                                     <  2:153099/150‑1 (MQ=255)
                             aaaCTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGcaca                                                                                                                     >  1:3504865/1‑150 (MQ=255)
                              aaCTTTCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACAt                                                                                                                    <  1:1605071/150‑1 (MQ=255)
                               aCTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACAtt                                                                                                                   >  2:386388/1‑150 (MQ=255)
                                cTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTg                                                                                                                  >  2:5657990/1‑150 (MQ=255)
                                cTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTg                                                                                                                  >  1:336370/1‑150 (MQ=255)
                                   ccAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATa                                                                                                               <  1:661331/150‑1 (MQ=255)
                                    cAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAg                                                                                                              >  2:5300294/1‑150 (MQ=255)
                                    cAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCCGGGATTGCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTTCAGCGCACATTGATAg                                                                                                              <  1:2450388/150‑1 (MQ=255)
                                     agagATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAgg                                                                                                             >  2:660800/1‑150 (MQ=255)
                                     agagATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAgg                                                                                                             >  2:3240761/1‑150 (MQ=255)
                                      gagaTCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGc                                                                                                            >  1:3959896/1‑150 (MQ=255)
                                      gagaTCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGc                                                                                                            <  1:4495337/150‑1 (MQ=255)
                                       agaTCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGcg                                                                                                           >  1:920010/1‑149 (MQ=255)
                                        gaTCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGcga                                                                                                          <  1:578995/150‑3 (MQ=255)
                                         aTCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCgaa                                                                                                         >  1:3241346/1‑150 (MQ=255)
                                              tCCGTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCgaatgaat                                                                                                    <  1:2259876/150‑1 (MQ=255)
                                              tCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCgaatgaat                                                                                                    <  2:5116512/150‑1 (MQ=255)
                                               cccTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATa                                                                                                   >  1:785861/1‑150 (MQ=255)
                                                ccTGCAAGTCAAACCGGTTAGTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATAc                                                                                                  <  2:4223318/150‑1 (MQ=255)
                                                 cTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACt                                                                                                 >  1:2021027/1‑150 (MQ=255)
                                                  tGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACtt                                                                                                <  2:785861/150‑1 (MQ=255)
                                                  tGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACtt                                                                                                <  1:4431558/150‑1 (MQ=255)
                                                     aaGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACttttt                                                                                             <  2:2465606/150‑1 (MQ=255)
                                                      aGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACtttttt                                                                                            >  2:4612466/1‑150 (MQ=255)
                                                       gTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACttttttt                                                                                           <  2:5619183/150‑1 (MQ=255)
                                                         cAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTgg                                                                                         >  1:2530202/1‑150 (MQ=255)
                                                          aaaGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGa                                                                                        <  1:2773707/150‑1 (MQ=255)
                                                          aaaGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGa                                                                                        >  1:1442636/1‑150 (MQ=255)
                                                            aGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATg                                                                                      >  1:2166957/1‑150 (MQ=255)
                                                            aGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATg                                                                                      <  2:2507442/150‑1 (MQ=255)
                                                                gTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAAt                                                                                  >  1:4388604/1‑150 (MQ=255)
                                                                 ttATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATa                                                                                 <  2:379984/150‑1 (MQ=255)
                                                                  tATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGTCGAATGAATACTTTTTTTGGATGAAATaa                                                                                >  1:5228861/1‑150 (MQ=255)
                                                                   aTTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATaaa                                                                               >  1:4121745/1‑150 (MQ=255)
                                                                       cGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCAt                                                                           >  2:5567756/1‑150 (MQ=255)
                                                                        gagagaTCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCAtc                                                                          <  2:1629942/150‑1 (MQ=255)
                                                                        gagagaTCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCAtc                                                                          >  2:4090273/1‑150 (MQ=255)
                                                                         agagaTCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCAtct                                                                         <  1:5657989/150‑1 (MQ=255)
                                                                          gagaTCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCAtctc                                                                        >  2:1478692/1‑150 (MQ=255)
                                                                          gagaTCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCAtctc                                                                        <  1:5440286/150‑1 (MQ=255)
                                                                            gaTCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATctcct                                                                      >  2:1777905/1‑150 (MQ=255)
                                                                             aTCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCCTGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATctcctc                                                                     <  2:1896133/150‑1 (MQ=255)
                                                                              tCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCg                                                                    <  2:3031483/150‑1 (MQ=255)
                                                                              tCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCg                                                                    <  2:498568/150‑1 (MQ=255)
                                                                              tCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCg                                                                    <  1:465035/150‑1 (MQ=255)
                                                                               cTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGt                                                                   >  1:2314980/1‑150 (MQ=255)
                                                                                ttGAAGACGCTGGACATGGAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTc                                                                  <  2:3504865/150‑1 (MQ=255)
                                                                                 tGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTcc                                                                 >  2:424512/1‑150 (MQ=255)
                                                                                  gAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCa                                                                >  2:1140264/1‑150 (MQ=255)
                                                                                       cGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGa                                                           >  2:4327300/1‑150 (MQ=255)
                                                                                           ggACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAACTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGa                                                       >  2:3120042/1‑150 (MQ=255)
                                                                                            gACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAg                                                      >  2:5042188/1‑150 (MQ=255)
                                                                                             aCATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAgc                                                     <  1:4612466/150‑1 (MQ=255)
                                                                                               aTGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAgcgc                                                   >  1:1836558/1‑150 (MQ=255)
                                                                                                tGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCt                                                  <  1:726926/150‑1 (MQ=255)
                                                                                                 gCAGCGTGATATCGCCGGCCTCGGATTCCGGCAAATGTAATTCATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCCAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCtt                                                 <  1:3788880/150‑1 (MQ=255)
                                                                                                 gCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCtt                                                 <  1:5361633/150‑1 (MQ=255)
                                                                                                   aGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCa                                               >  2:281055/1‑150 (MQ=255)
                                                                                                     cGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCAtt                                             <  2:2113006/150‑1 (MQ=255)
                                                                                                         atatAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGAt                                         >  1:2850105/1‑150 (MQ=255)
                                                                                                          tataGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGAtt                                        >  2:5210547/1‑150 (MQ=255)
                                                                                                              gCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCaa                                    >  1:5134673/1‑150 (MQ=255)
                                                                                                              gCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCaa                                    <  2:2042707/150‑1 (MQ=255)
                                                                                                                 ggCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCg                                 >  2:1759891/1‑150 (MQ=255)
                                                                                                                 ggCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCg                                 >  2:2241398/1‑150 (MQ=255)
                                                                                                                 ggCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCg                                 <  2:4725630/150‑1 (MQ=255)
                                                                                                                   ccTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCggc                               >  2:3716909/1‑148 (MQ=255)
                                                                                                                    cTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCggct                              >  1:3740984/1‑150 (MQ=255)
                                                                                                                    cTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCggct                              <  2:3401651/150‑1 (MQ=255)
                                                                                                                               gCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTAtc                   <  1:386388/150‑1 (MQ=255)
                                                                                                                               gCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTAtc                   <  2:1245724/150‑1 (MQ=255)
                                                                                                                                  aaTGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCa                >  2:4502611/1‑150 (MQ=255)
                                                                                                                                   aTGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGTGCGCTTCATTTGATTCCAACCGGCTGCCCGCGTATCGCaa               >  1:3075921/1‑150 (MQ=255)
                                                                                                                                    tGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCaaa              >  2:2582854/1‑150 (MQ=255)
                                                                                                                                    tGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCaaa              >  1:3008649/1‑150 (MQ=255)
                                                                                                                                      tAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATg            >  2:2140352/1‑150 (MQ=255)
                                                                                                                                      tAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATg            <  2:266816/150‑1 (MQ=255)
                                                                                                                                         ttGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGt         <  1:1230713/150‑1 (MQ=255)
                                                                                                                                              atTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACaa    >  1:2677421/1‑150 (MQ=255)
                                                                                                                                              atTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACaa    >  1:1702678/1‑150 (MQ=255)
                                                                                                                                                tCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAgg  >  2:4127552/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TATTACACGCTCAATCCCGATAAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGACTCCGGCAAATGTAATTGATATTCACTTTTCAAAAACTCTGCCAGCTCAAGCGCACATTGATAGGCAAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGACTGACTGCGTATCTCAAATGCGTGACAAGG  >  CP006881/3723931‑3724224

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A