Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A17 F55 I0 R1	217	81.2	3243344	95.0%	3081176	126.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP006881	3,723,505	C→T	100%	L107L (CTG→TTG)	U712_19195 →	putative transporter ywcJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	3,723,505	0	C	T	100.0%	146.5 / NA	44	L107L (CTG→TTG)	U712_19195	putative transporter ywcJ
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (23/21); total (23/21)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TCCGGCAGCCGCTGTCACATTCGGGGCCGCTATTCTGCTGATTGCTTACGGAGGCGGAGATTTATTTACTGGCAACACTTTTTATTTCACTTATACCGCGCTCCGAAAAAAAATTCGCTGGCGAGACACCCTGTACCTGTGGATGTCAAGCTATGCCGGCAATTTGATCGGCGCCATTCTATTTGCCATTCTGATCAGCGCGACAGGGCTTTTTGAGGAGGCTTCTGTTCATTCCTTTTTGATTCATCTGGCAGAGCA  >  CP006881/3723369‑3723626

tCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTTTGATGATTGCGTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATAACGCGCTCCGGAAAAAAATCAGCTGGCGCGGCACCCTCTACTtg                                                                                                                         >  5:70934/1‑139 (MQ=255)

  cGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTtgt                                                                                                                        <  2:45941/138‑1 (MQ=255)

           cTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCAATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAg                                                                                                              <  8:23717/139‑1 (MQ=255)

                   ttCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCg                                                                                                      >  4:354981/1‑139 (MQ=255)

                    agacagCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCgg                                                                                                     <  1:253033/134‑1 (MQ=255)

                             cTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAAt                                                                                            <  1:327015/139‑1 (MQ=255)

                                ttCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCgg                                                                                         <  8:65785/139‑1 (MQ=255)

                                     atgATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCa                                                                                    >  6:391035/2‑139 (MQ=255)

                                       gATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGCCACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCAtt                                                                                  >  4:215861/1‑139 (MQ=255)

                                        agtGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCAtt                                                                                  <  6:85262/136‑1 (MQ=255)

                                        aTTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTc                                                                                 >  1:15530/1‑139 (MQ=255)

                                        aTTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTc                                                                                 >  8:68011/1‑139 (MQ=255)

                                          tGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCAGGCAATTTAATCGGCGCCATTCtg                                                                               >  5:11856/1‑138 (MQ=255)

                                            cTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAAGTTAATCGGCGCCATTCTGtt                                                                             >  2:61716/1‑139 (MQ=255)

                                               aCGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATCCCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTg                                                                           <  2:3336/138‑1 (MQ=255)

                                               aCGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTg                                                                           <  3:354981/138‑1 (MQ=255)

                                               aCGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTg                                                                           <  1:298436/138‑1 (MQ=255)

                                               aCGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCAGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGAGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTg                                                                           <  5:176815/138‑1 (MQ=255)

                                                        gagaTTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGTCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTTAt                                                                 >  5:250983/1‑139 (MQ=255)

                                                                     cggCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCt                                                                                >  1:10212/2‑111 (MQ=255)

                                                                     cggCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCt                                                                                <  2:10212/110‑1 (MQ=255)

                                                                     cggCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACggga                                                    >  3:334204/2‑138 (MQ=255)

                                                                              cttttATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTgag                                           <  2:15530/138‑1 (MQ=255)

                                                                               ttttATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTgag                                           >  6:400168/1‑138 (MQ=255)

                                                                                 ttATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATCCTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTgagga                                         <  6:124469/138‑1 (MQ=255)

                                                                                 ttATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTgagga                                         <  8:197856/138‑1 (MQ=255)

                                                                                 ttATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTgagga                                         <  6:11856/138‑1 (MQ=255)

                                                                                        acctataCCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTg                                 >  5:699/4‑139 (MQ=255)

                                                                                              aCCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCAtt                           >  4:298328/1‑139 (MQ=255)

                                                                                                  cgcTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATGCTGCTCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTGATTCCtt                       >  4:232487/1‑139 (MQ=255)

                                                                                                  cgcTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGCTCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCtt                       >  6:159773/1‑139 (MQ=255)

                                                                                                  cgcTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGt                                <  5:152877/130‑1 (MQ=255)

                                                                                                  cgcTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGt                                >  6:152877/1‑130 (MQ=255)

                                                                                                  cgcTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCtt                       >  8:212764/1‑139 (MQ=255)

                                                                                                  cgcTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCtt                       >  1:223722/1‑139 (MQ=255)

                                                                                                  cgcTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCtt                       >  2:388709/1‑139 (MQ=255)

                                                                                                  cgcTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCtt                       >  2:77123/1‑139 (MQ=255)

                                                                                                  cgcTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGGTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTGTGGTCAttgcgt                       >  2:381141/1‑135 (MQ=255)

                                                                                                    cTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCtttt                     >  4:230449/1‑139 (MQ=255)

                                                                                                                  ccGCTGGCGCGACACCCTATACTTGGGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCa       <  1:381141/138‑1 (MQ=255)

                                                                                                                   agCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCa       <  5:83423/137‑1 (MQ=255)

                                                                                                                   agCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCa       <  5:400168/137‑1 (MQ=255)

                                                                                                                   agCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCa       <  2:264330/137‑1 (MQ=255)

                                                                                                                       ggCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGGAGAGca  <  1:388709/139‑1 (MQ=255)

TCCGGCAGCCGCTGTCACATTCGGGGCCGCTATTCTGCTGATTGCTTACGGAGGCGGAGATTTATTTACTGGCAACACTTTTTATTTCACTTATACCGCGCTCCGAAAAAAAATTCGCTGGCGAGACACCCTGTACCTGTGGATGTCAAGCTATGCCGGCAATTTGATCGGCGCCATTCTATTTGCCATTCTGATCAGCGCGACAGGGCTTTTTGAGGAGGCTTCTGTTCATTCCTTTTTGATTCATCTGGCAGAGCA  >  CP006881/3723369‑3723626

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A