Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A18 F53 I0 R2 221 66.0 2680176 94.9% 2543487 126.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 3,723,939 G→A 100% T251T (ACG→ACA)  U712_19195 → putative transporter ywcJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068813,723,9390GA100.0% 208.9 / NA 64T251T (ACG→ACA) U712_19195putative transporter ywcJ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (42/22);  total (42/22)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GTGCACATTCGCCATCTCACTTTTGATCGAGCACCCTGATACAGTGACAGTAATGGGAGCAGTCAGAAACTTAATTCCCGTTACACTCGGCAACCTGACCGCGGGAATGGTCATGATGGGCTGGATGTATTACACGCTCAATCCCGATAAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGACTCCGGCAAATGTAATTGA  >  CP006881/3723804‑3724071
                                                                                                                                       |                                                                                                                                    
gtgCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGCTGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACt                                                                                                                                    <  5:49638/138‑1 (MQ=255)
   cacaTTCGCCATCTCGCTTTTGATCTAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCACCGTTACGCTCGGCAATCTGACCGCGTGAATAGTTATGATGGGCTGGATGTACTACACACTGAAt                                                                                                                                >  8:111306/1‑139 (MQ=255)
   cacaTTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAAt                                                                                                                                >  1:250559/1‑139 (MQ=255)
   cacaTTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAAt                                                                                                                                >  6:243897/1‑139 (MQ=255)
   cacaTTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAAt                                                                                                                                >  6:142853/1‑139 (MQ=255)
                                 cccTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCa                                                                                                  <  3:32632/139‑1 (MQ=255)
                                    tGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTc                                                                                               >  6:292270/1‑139 (MQ=255)
                                            tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGAtcaataaa                                                                                                                   >  6:202116/1‑111 (MQ=255)
                                            tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGAtcaataaa                                                                                                                   <  5:202116/111‑1 (MQ=255)
                                            tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATaaaaa                                                                                                                 <  6:156797/113‑1 (MQ=255)
                                            tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATaaaaa                                                                                                                 >  5:156797/1‑113 (MQ=255)
                                            tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGAt                                                                                                    >  5:110956/1‑126 (MQ=255)
                                            tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGAt                                                                                                    <  6:110956/126‑1 (MQ=255)
                                            tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAg                                                                                       >  3:256407/1‑139 (MQ=255)
                                            tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAg                                                                                       >  1:97911/1‑139 (MQ=255)
                                            tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAg                                                                                       >  1:132145/1‑139 (MQ=255)
                                            tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAAT‑AAAAAACGTCCAGAGCTCACGACCTGCaat                                                                                       >  8:33437/1‑137 (MQ=255)
                                            tGACACTCATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCTGGAAGAGTAATGATGGGCTGGATGTAATACACACTGAATCCTGATCAATAAAAACACTTCCAGAGATCACTCCCTGCAAg                                                                                       >  2:110245/1‑139 (MQ=255)
                                              ccactgaTGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGTCAATCTGACCGCGGGAATAGTTATGATGTGCTGGATTTACTACACACTGAATCCTGATCAATAAAAAAAATTCAAGATATCACTACCTCCAAGt                                                                                      >  5:72186/7‑138 (MQ=255)
                                              acactgaTGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGAt                                                                                                    >  1:113226/7‑124 (MQ=255)
                                              acactgaTGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGAt                                                                                                    <  2:113226/118‑1 (MQ=255)
                                              acactgaTGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGt                                                                                      >  4:260374/7‑138 (MQ=255)
                                              acactgaTGGGAGCAGTCAGAAACTTAATCCACGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATCGGATGGATGTACTACACACTGAATCCTGATAAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGt                                                                                      >  8:151176/7‑138 (MQ=255)
                                                actgaTGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCaa                                                                                   <  5:243897/135‑1 (MQ=255)
                                                actgaTGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCa                                                                                    >  4:206131/5‑138 (MQ=255)
                                                actgaTGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCa                                                                                    >  3:26793/5‑138 (MQ=255)
                                                    aTGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCg                                                                               >  6:242600/1‑139 (MQ=255)
                                                    aTGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCg                                                                               >  3:302208/1‑139 (MQ=255)
                                                    aTGGGAGCAGTCAGAAACTTAATACCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAg                                                                                 <  7:78599/137‑1 (MQ=255)
                                                          gCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTAtt                                                                         >  1:291831/1‑139 (MQ=255)
                                                           cAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCa                                                                                                         <  6:229985/106‑1 (MQ=255)
                                                              tCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTcc                                                                       >  3:244620/1‑137 (MQ=255)
                                                                aGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCgaga                                                                   <  5:142853/139‑1 (MQ=255)
                                                                aGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCgag                                                                    <  4:244620/138‑1 (MQ=255)
                                                                 gAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCgagag                                                                  <  4:256407/139‑1 (MQ=255)
                                                                        aaTCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAGTCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTg                                                            >  7:282560/1‑138 (MQ=255)
                                                                         atccccGTTACGGCCGGAAATCTGACCGCGGAAATAGTTATGATGGGCTGTATGTACAACACACTCAAACCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGaa                                                          <  2:291831/136‑1 (MQ=255)
                                                                                     cTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACAt                                              >  6:126011/1‑139 (MQ=255)
                                                                                        ggCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATg                                             <  5:242600/137‑1 (MQ=255)
                                                                                        ggCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATg                                             <  2:97911/137‑1 (MQ=255)
                                                                                           aatcTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCg                                        <  3:206131/136‑1 (MQ=255)
                                                                                                  ccGCGGGAATAGTTATGATGGGCTGGATGTACTACACACtg                                                                                                                                   >  8:168683/1‑40 (MQ=255)
                                                                                                  ccGCGGGAATAGTTATGATGGGCTGGATGTACTACACACtg                                                                                                                                   <  7:168683/41‑2 (MQ=255)
                                                                                                  ccGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCgag                                                                    <  5:192692/104‑1 (MQ=255)
                                                                                                  ccGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCgag                                                                    >  6:192692/1‑104 (MQ=255)
                                                                                                  ccGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGata                                   >  5:155490/1‑137 (MQ=255)
                                                                                                       ggAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCgg                            >  5:314986/1‑139 (MQ=255)
                                                                                                              ttatgatgGGCTGCATGTACTACACACTGAATCCTGATCAATAAAAACACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGa                     <  7:245797/137‑1 (MQ=255)
                                                                                                                   atgGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGAc                                                       >  1:50028/1‑100 (MQ=255)
                                                                                                                   atgGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGAc                                                       <  2:50028/100‑1 (MQ=255)
                                                                                                                      ggCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGc              >  2:96389/1‑138 (MQ=255)
                                                                                                                      ggCTGGATGTACTACACACTGAATCCTGATCAAT‑AAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCa             >  8:3016/1‑138 (MQ=255)
                                                                                                                      ggCTGGATGTACTACACACAGAAACCTGATCAATAAAAAAACTTCCAGAGATCACACCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGAAGCGTGATATAGCCGGCCTCGGATTCCGGc              >  3:93681/1‑138 (MQ=255)
                                                                                                                       gCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCGAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTCAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCa             >  3:314427/1‑138 (MQ=255)
                                                                                                                        cTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGAc                                                       >  3:112858/1‑95 (MQ=255)
                                                                                                                        cTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGAc                                                       <  4:112858/95‑1 (MQ=255)
                                                                                                                        cTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCTGCCTCGGATTCCGGCaaa           >  4:319426/1‑139 (MQ=255)
                                                                                                                        cTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCaaa           >  1:331057/1‑139 (MQ=255)
                                                                                                                        cTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCaaa           >  6:3343/1‑139 (MQ=255)
                                                                                                                        cTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCaaa           >  1:191528/1‑139 (MQ=255)
                                                                                                                        cTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCaaa           >  8:180444/1‑139 (MQ=255)
                                                                                                                        cTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGAAAGTCACAGCGGTTATTCCGAGAGATCTTGAAGACTCTGGACATGCAGAGTGATATAGCCTGCGTAGGATTCCGTCaaa           >  6:176797/1‑139 (MQ=255)
                                                                                                                              ttaatACACACTTAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGGCAAAGCGGTTATTCCGAGATATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTaa      <  5:106372/134‑1 (MQ=255)
                                                                                                                                 ctACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAATACGCTGGACATGCAGCGTGATATAGCCGCCCTCGGCTTCCGGCAAAAGTAATTGa  >  6:106372/2‑139 (MQ=255)
                                                                                                                                       |                                                                                                                                    
GTGCACATTCGCCATCTCACTTTTGATCGAGCACCCTGATACAGTGACAGTAATGGGAGCAGTCAGAAACTTAATTCCCGTTACACTCGGCAACCTGACCGCGGGAATGGTCATGATGGGCTGGATGTATTACACGCTCAATCCCGATAAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGACTCCGGCAAATGTAATTGA  >  CP006881/3723804‑3724071

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A