Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A18 F53 I0 R2
|
221 |
66.0 |
2680176 |
94.9% |
2543487 |
126.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
CP006881 |
3,724,101 |
A→C |
100% |
L242L (CTT→CTG) |
U712_19200 ← |
SacPA operon antiterminator |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | CP006881 | 3,724,101 | 0 | A | C | 100.0%
| 239.5
/ ‑6.6
| 67 | L242L (CTT→CTG) | U712_19200 | SacPA operon antiterminator |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base C (28/38); total (29/38) |
| Rejected as polymorphism: E-value score below prediction cutoff. |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
TCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGACTCCGGCAAATGTAATTGATATTCACTTTTCAAAAACTCTGCCAGCTCAAGCGCACATTGATAGGCAAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGACTGACTGCGTATCTCAAATGCGTGACAAGGCGTTGAT > CP006881/3723965‑3724231
|
tCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTCGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCAAg > 7:126496/1‑139 (MQ=255)
caagagATCACTCCCTGAAAGTCAAAGCGGTTATTCAGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAg < 8:126496/136‑1 (MQ=255)
agagATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGAACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAgcgc < 6:277314/139‑1 (MQ=255)
aTCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACAt > 3:213183/1‑139 (MQ=255)
cACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACAtt > 8:88403/1‑138 (MQ=255)
tGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGc > 6:231738/1‑138 (MQ=255)
cAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGcga < 1:84445/138‑3 (MQ=255)
tCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGaa > 2:183851/1‑139 (MQ=255)
aGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACt > 5:275839/1‑139 (MQ=255)
tATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACttttttt > 2:305211/1‑139 (MQ=255)
ccGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGa < 4:213183/138‑1 (MQ=255)
ccGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGa < 4:26793/138‑1 (MQ=255)
cGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGAt < 5:126011/138‑1 (MQ=255)
agagaTCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGa > 7:168191/1‑138 (MQ=255)
agagaTCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGa > 6:135130/1‑138 (MQ=255)
agagaTCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTATGGAtta > 8:43034/1‑136 (MQ=255)
agaTCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAg > 2:180496/1‑98 (MQ=255)
agaTCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAg < 1:180496/98‑1 (MQ=255)
gaTCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATa < 7:88403/139‑1 (MQ=255)
gaTCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATa < 4:61630/139‑1 (MQ=255)
aTCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATa > 1:194123/1‑138 (MQ=255)
ttGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGc < 1:120284/139‑1 (MQ=255)
gAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGc < 7:2790/137‑1 (MQ=255)
gACGCTGGACATGCTGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACCTTGATAGGCGAATGAATACTTTTTTTGGATGAATTAAAGCAtctc < 7:151176/139‑1 (MQ=255)
gACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCAtctc < 4:130477/139‑1 (MQ=255)
gACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCAtctc < 8:168191/139‑1 (MQ=255)
gACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCAtctc < 1:96389/139‑1 (MQ=255)
cGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCATAAACTCCGCCAGCTCCAGCTCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATctcct < 7:43034/139‑1 (MQ=255)
cGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATctcct > 6:169526/1‑139 (MQ=255)
gCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTTATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATctcctc > 8:119642/1‑139 (MQ=255)
gCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATctcctc > 7:301084/1‑139 (MQ=255)
gCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATctcctc < 2:250559/139‑1 (MQ=255)
gCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTATTCAAAAAATCCGCCAGCTCCAGCGCACAATGATACGCGAATGAATACTTTTTTTGGATGAAATAAAGCATctcct > 4:199682/1‑138 (MQ=255)
tGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGt > 2:255738/1‑139 (MQ=255)
tGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTGTTTTTGGATGAAATAAAGCATCTCCTCGt > 6:83300/1‑139 (MQ=255)
gACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGt < 8:313661/137‑1 (MQ=255)
aCATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCa < 8:265534/139‑1 (MQ=255)
aCATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCa < 3:319426/139‑1 (MQ=255)
aTGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATa < 1:110245/139‑1 (MQ=255)
gCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATg > 1:312132/1‑139 (MQ=255)
gTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGa < 5:292270/139‑1 (MQ=255)
tGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAg < 8:33338/139‑1 (MQ=255)
tGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAg < 8:301084/139‑1 (MQ=255)
tGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAg > 1:213961/1‑139 (MQ=255)
tGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAg < 7:316416/139‑1 (MQ=255)
aGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCtt > 5:292490/1‑138 (MQ=255)
ccGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAg < 5:24440/131‑1 (MQ=255)
ccGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAg > 6:24440/1‑131 (MQ=255)
ccGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCAt > 1:177356/1‑139 (MQ=255)
ggCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCAttt > 8:153511/1‑139 (MQ=255)
cTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGa < 5:141667/92‑1 (MQ=255)
cTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGa > 6:141667/1‑92 (MQ=255)
attCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCaa < 6:275839/137‑1 (MQ=255)
tGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCggctgaccg < 1:257/139‑3 (MQ=255)
tAATTGATATTGATTTTTCAAAAACTCCTCCAGCTCCAGCGCACATTGATATGCGAATGAATACTTTTTTTGGATGATATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGAATCCAACCGGCTGACcgcg < 4:93681/139‑1 (MQ=255)
aTTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGt < 8:80420/93‑1 (MQ=255)
aTTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGt > 7:80420/1‑93 (MQ=255)
atatTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTAtcgc < 7:180444/139‑3 (MQ=255)
atatTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTAtcgc < 6:155490/139‑3 (MQ=255)
tatTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCa < 3:260374/139‑1 (MQ=255)
tCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAAt > 6:100558/1‑139 (MQ=255)
ttCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGa > 1:45930/1‑139 (MQ=255)
cgCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCAt < 6:96753/59‑1 (MQ=255)
cgCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCAt > 5:96753/2‑60 (MQ=255)
gCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGAt < 5:75753/139‑1 (MQ=255)
gCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGAt < 5:61550/139‑1 (MQ=255)
gCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGAt < 6:141470/139‑1 (MQ=255)
|
TCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGACTCCGGCAAATGTAATTGATATTCACTTTTCAAAAACTCTGCCAGCTCAAGCGCACATTGATAGGCAAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGACTGACTGCGTATCTCAAATGCGTGACAAGGCGTTGAT > CP006881/3723965‑3724231
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
