Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A19 F53 I0 R3 198 62.2 2365906 95.5% 2259440 127.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 3,723,558 T→C 100% I124I (ATT→ATC)  U712_19195 → putative transporter ywcJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068813,723,5580TC100.0% 209.1 / NA 56I124I (ATT→ATC) U712_19195putative transporter ywcJ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (34/22);  total (34/22)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CGGAGATTTATTTACTGGCAACACTTTTTATTTCACTTATACCGCGCTCCGAAAAAAAATTCGCTGGCGAGACACCCTGTACCTGTGGATGTCAAGCTATGCCGGCAATTTGATCGGCGCCATTCTATTTGCCATTCTGATCAGCGCGACAGGGCTTTTTGAGGAGGCTTCTGTTCATTCCTTTTTGATTCATCTGGCAGAGCACAAAATGGAGCCGCCCGCTTCCGAACTGTTTTTCAGAGGGATGCTGTGCAATTGGCTTGTGTGCC  >  CP006881/3723423‑3723691
                                                                                                                                       |                                                                                                                                     
cGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCt                                                                                                                                     >  2:8030/1‑138 (MQ=255)
               cggCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCAGTCTGTTTGCCATCCTGATCAGCGCGACggga                                                                                                                     >  6:43266/2‑138 (MQ=255)
               cggCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCAGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCCACggga                                                                                                                     >  1:273161/2‑138 (MQ=255)
                        cttttATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGATTTTTTgag                                                                                                            <  2:166532/138‑1 (MQ=255)
                        cttttATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTgag                                                                                                            <  1:20772/138‑1 (MQ=255)
                        cttttATTTCACCTAAACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTgag                                                                                                            <  2:205303/138‑1 (MQ=255)
                                            cgcTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCtt                                                                                        >  7:104338/1‑139 (MQ=255)
                                            cgcTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCtt                                                                                        >  5:107685/1‑139 (MQ=255)
                                            cgcTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGGTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTccttcctt                                                                                        >  1:87996/1‑139 (MQ=255)
                                              cTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTgagga                                                                                                          >  1:223968/1‑119 (MQ=255)
                                              cTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTgagga                                                                                                          <  2:223968/119‑1 (MQ=255)
                                              cTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTATGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCtttt                                                                                      >  4:446/1‑139 (MQ=255)
                                                             agCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCa                                                                        <  3:446/137‑1 (MQ=255)
                                                                        cacCCTATACTGGTGCATGGCAAGCTAAGCCGGCAATTTAATCGGCCCCATTCTGTTTGCCAACCTGAGCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCCGAGCACAAAATg                                                            <  5:75091/139‑1 (MQ=255)
                                                                             tatACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACggg                                                                                                                      <  5:168469/74‑1 (MQ=255)
                                                                             tatACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACggg                                                                                                                      >  6:168469/3‑76 (MQ=255)
                                                                             tatACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCGGTTCATTCCGTTTTGATTCATTTGGCAGAGCACAAAATTGAgcc                                                       >  1:210189/3‑139 (MQ=255)
                                                                               tACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAgccgc                                                     >  4:208338/1‑139 (MQ=255)
                                                                                 cttgtgGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAgccgccg                                                   >  7:21525/3‑138 (MQ=255)
                                                                                    gtgGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGGCGGGACTTTTTGAGGAGGCGTCTGGTCATTGCTTTTTGAGTGATTTGGCTCAGCCACAAATGGAgccgccgggt                                                >  2:149509/1‑135 (MQ=255)
                                                                                    gtgGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTgagga                                                                                                          >  5:128322/1‑81 (MQ=255)
                                                                                    gtgGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTgagga                                                                                                          <  6:128322/81‑1 (MQ=255)
                                                                                    gtgGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCa                                                                               <  4:270324/108‑1 (MQ=255)
                                                                                    gtgGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCa                                                                               >  3:270324/1‑108 (MQ=255)
                                                                                    gtgGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCt                                                >  3:193903/1‑139 (MQ=255)
                                                                                     tgGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGCCGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGCGccgcccgcgt                                               >  4:18177/1‑137 (MQ=255)
                                                                                     tgGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGAc                                                                                                                         <  4:166532/65‑1 (MQ=255)
                                                                                     tgGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGAc                                                                                                                         >  3:166532/1‑65 (MQ=255)
                                                                                     tgGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTTATTCCTTTTTGATTCATTTGGCAGAGCACAAAATTGAGccgccggcgt                                               >  1:273137/1‑137 (MQ=255)
                                                                                     tgGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCTTTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAgccgcc                                                    <  4:115768/134‑1 (MQ=255)
                                                                                     tgGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGca                                                                   <  7:182624/119‑1 (MQ=255)
                                                                                     tgGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGca                                                                   >  8:182624/1‑119 (MQ=255)
                                                                                     tgGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAgccgcc                                                    >  3:115768/1‑134 (MQ=255)
                                                                                     tgGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCtt                                               >  3:42736/1‑139 (MQ=255)
                                                                                     tgGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCtt                                               >  7:61797/1‑139 (MQ=255)
                                                                                     tgGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCATTTTTGATTCCTTTTGCAGAGAACAAAATGGAGCCGACGGCtt                                               >  6:32183/1‑139 (MQ=255)
                                                                                     tgGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTAATTCCTTTTTTATTAATTTGGCAGAGCACAAAATTGATCCGCCGGCtt                                               >  2:289687/1‑139 (MQ=255)
                                                                                     tgGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCAACCTGATCAGCGCGACGGGACTGTTTGAGGAGCCTTCTGGTCATTCATTTTTGAGTCATTTGTCAGAGCACAAAATGGACCAGCCGGCt                                                >  5:87250/1‑138 (MQ=255)
                                                                                       gATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTcc                                             >  2:86838/1‑139 (MQ=255)
                                                                                       gATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTcc                                             >  5:100340/1‑139 (MQ=255)
                                                                                       gATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTcc                                             >  2:169034/1‑139 (MQ=255)
                                                                                       gATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTATGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGGAGAGCACAAAATGGAGCCGCCGGCTTcc                                             >  1:39241/1‑139 (MQ=255)
                                                                                        aTGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCg                                            <  8:21525/139‑1 (MQ=255)
                                                                                         tGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCg                                            >  7:42011/1‑138 (MQ=255)
                                                                                            cAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGaatt                                        >  3:24481/1‑137 (MQ=255)
                                                                                            cAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGaatt                                        >  7:26948/1‑137 (MQ=255)
                                                                                                   tGCCGGCAATTTACTCGGCGCCATGCGGGTGGCCATCCTGAGCCGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCCGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGttttt                                  <  2:87996/138‑1 (MQ=255)
                                                                                                   tGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGttttt                                  <  3:276504/138‑1 (MQ=255)
                                                                                                                 tCGGCGCCATTCTGTTTGCCATCGTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTAATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCtgtg                   <  6:150026/139‑1 (MQ=255)
                                                                                                                   ggCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCa                 <  1:86838/139‑1 (MQ=255)
                                                                                                                    gcgcCATTGTGTTTGCCATCCTTATCAGCGCGACGGGTCTTTTTGAGGAGCGTTCTGTTCATTCCTTTTTTATTCATTTGGAAGAGCACAAAAGGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCaa                <  2:39241/139‑1 (MQ=255)
                                                                                                                    gcgcCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCaa                <  8:42011/139‑1 (MQ=255)
                                                                                                                    gcgcCATACTGTTTGCACTCCTTATCACCGCGAGGGGACTTTTTGAGGCGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCaa                <  4:256644/139‑1 (MQ=255)
                                                                                                                      gcCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAAtt              <  8:61797/139‑1 (MQ=255)
                                                                                                                               tttGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTTGCAGAGCACAAAATGGAGCCGCCGGCGTCCGAATTTTTTTTAAGAGGTATTGTGTGGAATTTGCgtgtgt     >  1:137626/1‑139 (MQ=255)
                                                                                                                                   ccATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCATAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGcc  <  1:169034/138‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                     
CGGAGATTTATTTACTGGCAACACTTTTTATTTCACTTATACCGCGCTCCGAAAAAAAATTCGCTGGCGAGACACCCTGTACCTGTGGATGTCAAGCTATGCCGGCAATTTGATCGGCGCCATTCTATTTGCCATTCTGATCAGCGCGACAGGGCTTTTTGAGGAGGCTTCTGTTCATTCCTTTTTGATTCATCTGGCAGAGCACAAAATGGAGCCGCCCGCTTCCGAACTGTTTTTCAGAGGGATGCTGTGCAATTGGCTTGTGTGCC  >  CP006881/3723423‑3723691

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A