Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A15 F58 I0 R3
|
247 |
66.2 |
2815674 |
94.9% |
2672074 |
126.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
CP006881 |
3,724,902 |
A→C |
100% |
A89A (GCT→GCG) |
U712_19205 ← |
Uncharacterized protein ywcI |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | CP006881 | 3,724,902 | 0 | A | C | 100.0%
| 120.6
/ NA
| 37 | A89A (GCT→GCG) | U712_19205 | Uncharacterized protein ywcI |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (17/20); total (17/20) |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
TCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAGTACTTTATAGATTTTCAAGAGGCTAT‑CTCTCCCGCCAATTAGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCAGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTTTGTTTTTCTTCCTCATTCACAGCCATCCGCCACTCATAAAACGAAACAAGTCCCGCGGC > CP006881/3724768‑3725036
|
tCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTAT‑CTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGct < 6:122128/138‑1 (MQ=255)
tCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTAT‑CTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGct < 2:129167/138‑1 (MQ=255)
atcatcCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTCTAGATTTTCAAGAGGTTAT‑CTCTCCCGGCAATTTGCTTTTGACTGGCATTTTACCTCCTTTTGACCTCCATGTAAAGATGCGGGCCCGCtctctc < 8:298792/138‑1 (MQ=255)
atcatcCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTAT‑CTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCtctctc < 7:200576/138‑1 (MQ=255)
atcatcCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTAT‑CTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTcc > 5:248601/1‑139 (MQ=255)
tttATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTAT‑CTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGct < 1:136733/123‑1 (MQ=255)
tttATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTAT‑CTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGct > 2:136733/1‑123 (MQ=255)
tattaAAGCCGCATTGATGTTTAAAACTTTATAGATTTTCAAGAGGTTAT‑CTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCCTGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGa < 1:87425/138‑1 (MQ=255)
attaAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTAT‑CTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGAt > 7:170421/1‑138 (MQ=255)
aGCCGCATTGTTGTTTAATACTTTATAGAATTTCAAGAGGTTAT‑CTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCGATGTAAAGTTGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTg < 8:299902/139‑1 (MQ=255)
gCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTAT‑CTCTCCCGCCAATTTGCTTTTGCCTGGGATTTTCCATCCTTTAGACCGCCATGTAAAGATCCGGGCCCGCTCTCTCCTTCTTTGGCGATTCTTTg > 7:153947/1‑138 (MQ=255)
gCCGCATTGTTGATTAATACTTTATAGATTTTCAAGAGGTTAT‑CTCTCCCGCCAATTAGCTTTTGACTGGGATTTTACGTCCTTTTGACCGACATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTg > 4:140215/1‑138 (MQ=255)
cATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTAT‑CTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGc > 8:155547/1‑139 (MQ=255)
cATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTAT‑CTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGc > 3:181944/1‑139 (MQ=255)
cATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTAT‑CTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGc < 6:146440/139‑1 (MQ=255)
ttgttTAATACTTTATAGATTTTCAAGAGG‑TATGCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACAGCCATGTAAATATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCa < 2:137538/139‑1 (MQ=255)
gttTAATACTTTATAGATTTTCAAGAGGTTAT‑CTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCaa > 1:47710/1‑138 (MQ=255)
taatACTTTATAGATTTTCAAGAGGTTAT‑CTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCTCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACa > 1:311721/4‑138 (MQ=255)
cTTTATAGATTTTCAAGAGGTTAT‑CTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACAtcttct > 1:5284/1‑139 (MQ=255)
tataGATTTTCAAGAGGTTAT‑CTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTTCAAACATCTTCtttt > 6:328915/1‑139 (MQ=255)
tataGATTTTCAAGAGGTTAT‑CTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCa < 4:83389/126‑1 (MQ=255)
tataGATTTTCAAGAGGTTAT‑CTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCa > 3:83389/1‑126 (MQ=255)
aTTTTCAAGAGGTTAT‑CTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATaat > 8:295033/1‑138 (MQ=255)
tttCAAGAGGTTAT‑CTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAgc > 7:266139/1‑139 (MQ=255)
aaGAGGTTAT‑CTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCgcgg < 2:47710/139‑1 (MQ=255)
aaGAGGGAAT‑CTCTCCCGCCAATTGGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGcgcg < 1:324611/138‑1 (MQ=255)
cccGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCtttc > 5:81992/1‑138 (MQ=255)
aTTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGctttt < 6:248601/139‑1 (MQ=255)
cTTTTGACTGGGATTTTACCTCCTTTTGACCTCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTttcttc < 2:311721/138‑1 (MQ=255)
cTGGGCTTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTca < 1:253592/139‑1 (MQ=255)
cTGGGATTTTAGCTCCTTTTGACAGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTca < 8:153947/139‑1 (MQ=255)
cTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTca < 5:183995/139‑1 (MQ=255)
ggATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAg > 5:346316/1‑139 (MQ=255)
ctcctTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGcc < 3:92246/139‑1 (MQ=255)
aTGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTCTAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAACACGAAACGAGTcct < 8:170421/139‑2 (MQ=255)
cGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTAAACAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCtgcg < 4:181944/139‑1 (MQ=255)
ggCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCGTTCGCTTTATGCGTTTATTCCGCATTAACAGCCCTCCCCCAATCATAAAACGAAACGAGTCCAGCGGc > 2:4189/1‑139 (MQ=255)
|
TCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAGTACTTTATAGATTTTCAAGAGGCTAT‑CTCTCCCGCCAATTAGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCAGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTTTGTTTTTCTTCCTCATTCACAGCCATCCGCCACTCATAAAACGAAACAAGTCCCGCGGC > CP006881/3724768‑3725036
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
