Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A13 F56 I0 R1 198 55.3 2323794 95.3% 2214575 126.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 3,723,933 T→C 100% Y249Y (TAT→TAC)  U712_19195 → putative transporter ywcJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068813,723,9330TC100.0% 208.2 / ‑6.6 58Y249Y (TAT→TAC) U712_19195putative transporter ywcJ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (36/21);  minor base A (0/1);  total (36/22)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ATATGTGCACATTCGCCATCTCACTTTTGATCGAGCACCCTGATACAGTGACAGTAATGGGAGCAGTCAGAAACTTAATTCCCGTTACACTCGGCAACCTGACCGCGGGAATGGTCATGATGGGCTGGATGTATTACACGCTCAATCCCGATAAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGACTCCGGCA  >  CP006881/3723800‑3724060
                                                                                                                                     |                                                                                                                               
atatGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGGTATGATGGGCTGGATGTACTaca                                                                                                                             >  4:65716/1‑138 (MQ=255)
       cacaTTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAAt                                                                                                                     >  3:150685/1‑139 (MQ=255)
       cacaTTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGAGGTACTACACACTGAAt                                                                                                                     >  4:284847/1‑139 (MQ=255)
              gCCATCTCACTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGatc                                                                                                              <  2:82286/139‑2 (MQ=255)
                             aTCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGTGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTcc                                                                                               <  5:55292/139‑1 (MQ=255)
                                       cTGATACAGTGACACGGATGGGAGCAGTCAGAAACTTAATCCCCGTTCCGCGCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACt                                                                                     <  4:66346/139‑1 (MQ=255)
                                              aGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGc                                                                               >  8:246749/1‑138 (MQ=255)
                                                tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGTGAATAGTTATGATGGTCTCGATGTACTACACAATGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGc                                                                               >  3:18927/1‑136 (MQ=255)
                                                tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGc                                                                               >  4:73316/1‑136 (MQ=255)
                                                tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAg                                                                            >  8:166483/1‑139 (MQ=255)
                                                tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAg                                                                            >  2:216033/1‑139 (MQ=255)
                                                tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAg                                                                            >  3:24047/1‑139 (MQ=255)
                                                tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAg                                                                            >  7:135058/1‑139 (MQ=255)
                                                tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAg                                                                            >  4:271846/1‑139 (MQ=255)
                                                tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAg                                                                            >  2:206603/1‑139 (MQ=255)
                                                tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAg                                                                            >  5:57971/1‑139 (MQ=255)
                                                tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAg                                                                            >  3:66346/1‑139 (MQ=255)
                                                tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAg                                                                            >  2:153965/1‑139 (MQ=255)
                                                tGACACTGATGGGAGCAGACAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGCAATACATATGATCGGCTGGATGTACTACACACTGAATCCTCATCAATAAAAAAACATCCAGAGATCACTCCCTGc                                                                               >  4:39382/1‑136 (MQ=255)
                                                  acactgaTGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCGTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGt                                                                           >  4:24859/7‑138 (MQ=255)
                                                    actgaTGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCa                                                                         >  3:78532/5‑138 (MQ=255)
                                                    actgaTGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCa                                                                         >  6:97498/5‑138 (MQ=255)
                                                    actgaTGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCa                                                                         >  8:11015/5‑138 (MQ=255)
                                                        aTGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGAAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCg                                                                    <  4:150685/139‑1 (MQ=255)
                                                             aGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTAt                                                               >  4:156783/1‑139 (MQ=255)
                                                             aGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTAt                                                               >  4:58273/1‑139 (MQ=255)
                                                                aGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTcc                                                            >  6:51232/1‑139 (MQ=255)
                                                                aGTCAGAAACTTAATCCCAGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGAGGGGCTGGATGTACTACACACTGCATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTcc                                                            >  1:176927/1‑139 (MQ=255)
                                                                  tCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTcc                                                            >  1:225017/1‑137 (MQ=255)
                                                                  tCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTcc                                                            >  4:222726/1‑137 (MQ=255)
                                                                   cAGAAACTTAATCCCCGTTACGCGCGGCATTCTGACCGCGGGAATATGTATGATGGGCTGGATTAACTACACCCTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCgag                                                         <  2:176927/139‑1 (MQ=255)
                                                                    aGAAACTTAATCGCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCgag                                                         <  7:166483/138‑1 (MQ=255)
                                                                    aGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCgag                                                         <  8:274932/138‑1 (MQ=255)
                                                                    aGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCgag                                                         <  3:222726/138‑1 (MQ=255)
                                                                            aaTCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAAAAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTg                                                 >  7:36391/1‑138 (MQ=255)
                                                                             atcccggTTACGCGCGGCAATCTGACCGCGGGAATAGTTATGATGGGGTGGATGTACAACACAGTTAATCCTTATAAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGa                                                <  3:271846/132‑1 (MQ=255)
                                                                              tccccGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGCTAATTCCGAGAGATCTTg                                                 >  7:175442/3‑136 (MQ=255)
                                                                                  cGTTACGATCGGCAATATGACGGAGGGAATAGTTTTGTTGGGCTGGTTGTAATAAACAGTGAAACCTGATAAATAAAAACACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGc                                          <  8:135058/139‑1 (MQ=255)
                                                                                    ttACGCTCGGCATTCTGAGCGCGTGAATAGTTTTGATGGGCTGGATGTTCTACAAACTGATTCCTGATCATTAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTg                                        <  8:175442/139‑1 (MQ=255)
                                                                                            ggCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCgag                                                         >  8:107129/1‑114 (MQ=255)
                                                                                            ggCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCgag                                                         <  7:107129/114‑1 (MQ=255)
                                                                                             gCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAg                               <  4:24047/139‑1 (MQ=255)
                                                                                                    gACCGCGGGAATAGTTATGATGGTCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGata                        >  6:53881/1‑139 (MQ=255)
                                                                                                    gACCGAGGGAATAGTAATGATGGGCTTGATGTACTACACAATTAATACTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGAAAGACAAAGCTTATATTCCGAGAGATCTTGAAGACTCTGGACATGAAGCGTGat                         >  4:9978/1‑138 (MQ=255)
                                                                                                     aCCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCGTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGat                         <  3:24859/137‑1 (MQ=255)
                                                                                                      ccGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGata                        >  3:133488/1‑137 (MQ=255)
                                                                                                        gcgGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTTATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGc                    <  3:65716/139‑1 (MQ=255)
                                                                                                        gcgGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGc                    <  7:161599/139‑1 (MQ=255)
                                                                                                        gcgGGAATAGTTATGAGGGGCTGGATGAACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGc                    <  3:284847/139‑1 (MQ=255)
                                                                                                                       atgGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGc                              <  7:82292/114‑1 (MQ=255)
                                                                                                                       atgGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGc                              >  8:82292/1‑114 (MQ=255)
                                                                                                                       atgGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCg                             <  6:12572/115‑1 (MQ=255)
                                                                                                                       atgGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCg                             >  5:12572/1‑115 (MQ=255)
                                                                                                                         gggCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGc   <  3:73316/139‑1 (MQ=255)
                                                                                                                          ggCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGc   <  5:131327/138‑1 (MQ=255)
                                                                                                                          ggCTGGATGTACTACACACTGAATCCTGATCAAT‑AAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCa  >  8:252311/1‑138 (MQ=255)
                                                                                                                            cTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCgag                                                         >  6:273857/1‑82 (MQ=255)
                                                                                                                            cTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCgag                                                         <  5:273857/82‑1 (MQ=255)
                                                                                                                                     |                                                                                                                               
ATATGTGCACATTCGCCATCTCACTTTTGATCGAGCACCCTGATACAGTGACAGTAATGGGAGCAGTCAGAAACTTAATTCCCGTTACACTCGGCAACCTGACCGCGGGAATGGTCATGATGGGCTGGATGTATTACACGCTCAATCCCGATAAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGACTCCGGCA  >  CP006881/3723800‑3724060

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A