Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A13 F56 I0 R1 198 55.3 2323794 95.3% 2214575 126.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 3,724,194 A→G 100% S211S (AGT→AGC)  U712_19200 ← SacPA operon antiterminator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068813,724,1940AG100.0% 201.9 / NA 56S211S (AGT→AGC) U712_19200SacPA operon antiterminator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (32/24);  total (32/24)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AATGTAATTGATATTCACTTTTCAAAAACTCTGCCAGCTCAAGCGCACATTGATAGGCAAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGACTGACTGCGTATCTCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTCTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATG  >  CP006881/3724061‑3724329
                                                                                                                                     |                                                                                                                                       
aaTGTAATTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCggctgac                                                                                                                                    <  7:246749/139‑1 (MQ=255)
     aaTTGATATTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGCCcgcg                                                                                                                                >  1:161610/1‑138 (MQ=255)
           tatTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCa                                                                                                                         <  4:87724/139‑1 (MQ=255)
            atTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCa                                                                                                                         >  1:90586/1‑138 (MQ=255)
            atTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCa                                                                                                                         >  7:207201/1‑138 (MQ=255)
            atTCATTTTTCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCa                                                                                                                         >  2:244279/1‑138 (MQ=255)
                     tCAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGa                                                                                                                >  7:5015/1‑138 (MQ=255)
                      cAAAAACTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGAc                                                                                                               <  1:36845/138‑1 (MQ=255)
                           aCTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCg                                                                                                         <  1:206603/139‑1 (MQ=255)
                           aCTCCGCCAGCTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCg                                                                                                         <  2:285929/139‑1 (MQ=255)
                                     cTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCTTTTGATTCCAACCggctgaccg                                                                                                                                  >  8:142998/1‑102 (MQ=255)
                                     cTCCAGCGCACATTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCggctgaccg                                                                                                                                  <  7:142998/104‑3 (MQ=255)
                                                aTTGATAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGtttt                                                                                     >  1:249146/1‑138 (MQ=255)
                                                    aTAGGCGAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATc                                                                                 <  2:30080/138‑1 (MQ=255)
                                                            aTGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACa                                                                                                              >  3:90908/1‑101 (MQ=255)
                                                            aTGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACa                                                                                                              <  4:90908/101‑1 (MQ=255)
                                                            aTGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTACATTTGATTCCAACCGGCAGACCGCGTATCGCAAATGCGTGACAAGCCGTTCATAGGAAATGCTGTTTTCATCCACCTTTc                                                                         >  8:163278/1‑138 (MQ=255)
                                                                      tttttGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTa                                                               <  5:184165/138‑1 (MQ=255)
                                                                      ttattGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTACATCCACCTTTCGATTAAAGTAt                                                              >  6:159154/4‑139 (MQ=255)
                                                                              tGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTAt                                                      >  6:258096/1‑139 (MQ=255)
                                                                                aaaTAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTAtt                                                     <  3:216594/138‑1 (MQ=255)
                                                                                           ctcctcGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCAtt                                         >  7:30515/1‑139 (MQ=255)
                                                                                           ctcctcGTCCATACGATGGAGCGCTTCATTTGATTCAAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCAtt                                         >  2:88462/1‑139 (MQ=255)
                                                                                                cGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTAGCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATGTTCTCTATCATTTCttt                                    >  8:216547/1‑139 (MQ=255)
                                                                                                  tCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGa                                  >  4:79991/1‑139 (MQ=255)
                                                                                                     aTACGATGGAGCGCTTCATTTGATTCTAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCa                               <  1:88462/139‑1 (MQ=255)
                                                                                                     aTACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGGGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCa                               <  8:60990/139‑1 (MQ=255)
                                                                                                      tACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCAt                              >  6:116691/1‑139 (MQ=255)
                                                                                                      tACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCAt                              >  6:200308/1‑139 (MQ=255)
                                                                                                      tACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCAt                              >  3:2584/1‑139 (MQ=255)
                                                                                                      tACGATGGAGCGCTTCATTTGACTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATGATTTATTTGATCAt                              >  4:149030/1‑139 (MQ=255)
                                                                                                       aCGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCGACCTTTCGATTAAAGTATTGTTTTTTTTTCTAAATCATTTTTTTGATCaag                             >  2:270332/1‑137 (MQ=255)
                                                                                                        cGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATgg                            <  2:161610/139‑1 (MQ=255)
                                                                                                           tGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGAc                                                                                                               >  8:1406/1‑53 (MQ=255)
                                                                                                           tGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGAc                                                                                                               <  7:1406/53‑1 (MQ=255)
                                                                                                           tGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTTTTTTATCGATACTTTCTTTGTTCATGGTCg                         >  2:133723/1‑139 (MQ=255)
                                                                                                           tGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCg                         >  5:175733/1‑139 (MQ=255)
                                                                                                           tGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCg                         >  8:134676/1‑139 (MQ=255)
                                                                                                           tGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCg                         >  6:278499/1‑139 (MQ=255)
                                                                                                             gAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTa                       <  5:234252/139‑1 (MQ=255)
                                                                                                              aGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATc                                                                                 <  1:64951/80‑1 (MQ=255)
                                                                                                              aGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATc                                                                                 >  2:64951/1‑80 (MQ=255)
                                                                                                              aGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTAt                      >  4:55311/1‑139 (MQ=255)
                                                                                                                cgcTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGc                    >  2:165716/1‑139 (MQ=255)
                                                                                                                  cTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCtt                  <  7:134676/139‑1 (MQ=255)
                                                                                                                  cTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCtt                  <  4:2584/139‑1 (MQ=255)
                                                                                                                   ttCATTTGATTCCATCCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTAATCCCCCTTTCGAATAAAGTATTGTTTTATTTTCACTATCATTTCTTTTATCTTGGTCGTATGCtt                  >  3:104049/1‑138 (MQ=255)
                                                                                                                   ttCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCtt                  >  7:283475/1‑138 (MQ=255)
                                                                                                                     cATTTGATTCCAACCGGCTGACCGCTTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATTCGGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTGTGAGAATTGTCGTATGCTTcct               >  6:43128/1‑137 (MQ=255)
                                                                                                                     cATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAg               >  8:178506/1‑139 (MQ=255)
                                                                                                                       tttGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAgcg             <  5:257960/139‑1 (MQ=255)
                                                                                                                       tttGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAgcg             <  8:5015/139‑1 (MQ=255)
                                                                                                                         tGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAgcgc            <  6:175733/138‑1 (MQ=255)
                                                                                                                           aTTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTg          <  6:135615/138‑1 (MQ=255)
                                                                                                                            ttCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATACAACTTTCGATTAAAGTCTTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGaa        <  5:43128/139‑1 (MQ=255)
                                                                                                                               cAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATAc     <  6:263568/139‑1 (MQ=255)
                                                                                                                                 aCCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACAt   >  5:158897/1‑139 (MQ=255)
                                                                                                                                  ccGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATg  <  7:256383/139‑1 (MQ=255)
                                                                                                                                  ccGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATg  <  8:283475/139‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                       
AATGTAATTGATATTCACTTTTCAAAAACTCTGCCAGCTCAAGCGCACATTGATAGGCAAATGAATACTTTTTTTGGATGAAATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGACTGACTGCGTATCTCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTCTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATG  >  CP006881/3724061‑3724329

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A