Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A14 F56 I20 R2
|
1958 |
92.1 |
3575798 |
91.1% |
3257551 |
126.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
CP006881 |
2,173,945 |
T→C |
L327L (CTA→CTG) |
U712_10875 ← |
CCA‑adding enzyme |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | CP006881 | 2,173,945 | 0 | T | C | 98.2%
| 171.1
/ NA
| 55 | L327L (CTA→CTG) | U712_10875 | CCA‑adding enzyme |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (23/31); minor base G (0/1); total (23/32) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GCTCGATCCGCTGTAATTCTTCCGACACCCATTTTCCGGCAGGCCGGTTACGAAGCGCTAGTAAATCTTTACCCGTGATATCAAGATCCCTAAGGCTTTTGATCGGCAGGTTTTGATAAGTATTTTGAATGTCCTTTAGTTTTTCATCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAAAGAAGTGATTTTTCCCCGGCTTTGTACATTGACATAGTGTCAAGGCTCTGGCCAAACGTATGGGAAATGTGAATC > CP006881/2173809‑2174077
|
gCTCGATCCACTGTAATTCTTCTGACACCCATTTTCCGGCCGGCCGGTTTCGAAGCGCAAGCAAGTCCTTACCCGTGATATCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAg > 1:306096/1‑139 (MQ=255)
gCTCGATCCACTGTAATTCTTCTGACACCCATTTTCCGGCCGGCCGGTTTCGAAGCGCAAGCAAGTCCTTACCCGTGATATCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAg > 2:18396/1‑139 (MQ=255)
cTCGATCCACTGTAATTCTTCTGACACCCATTTTCCGGCCGGCCGGTTTCGAAGCGCAAGCAAGTCCTTACCCGTGATATCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGt < 5:440005/139‑1 (MQ=255)
tCGATCCACTGTAATTCTTCTGACACCCATTTTCCGGCCGGCCGGTTTCGAAGCGCAAGCAAGTCCTTACCCGTGATATCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGtt > 8:132528/1‑139 (MQ=255)
ctgACACCCATTTTCCGGCCGGCCGGTTTCGAAGCGCAAGCAAGTCCTTACCCGTGATATCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTc > 7:392584/3‑139 (MQ=255)
ctgACACCCATTTTCCGGCCGGCCGGTTTCGAAGCGCAAGCAAGTCCTTACCCGTGATATCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTc > 6:198396/3‑139 (MQ=255)
gCCGGTTTCGAAGCGCAAGCAAGTCCTTACCCGTGATATCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGatat > 6:253246/1‑139 (MQ=255)
gCCGGTTTCGAAGCGCAAGCAAGTCCTTACCCGTGATATCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGatat > 7:308515/1‑139 (MQ=255)
tttcGAAGCGCAAGCAAGTCCTTACCCGTGATATCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTgg < 4:412317/136‑1 (MQ=255)
aaGCGCAAGCAAGTCCTTACCCGTGATATCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCt < 7:253740/139‑1 (MQ=255)
cttACCCGTGATATCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCttt < 2:306096/137‑1 (MQ=255)
cttACCCGTGATATCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCttt < 8:182370/137‑1 (MQ=255)
cttACCCGTGATATCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCttt < 8:392584/137‑1 (MQ=255)
aCCCGTGATATCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCttt < 6:62259/135‑1 (MQ=255)
aCCCGTGATATCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCttt > 5:62259/1‑135 (MQ=255)
cccGTGATATGAAGATCCTTAAGGATTTTGATAGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTtcc < 7:322641/139‑1 (MQ=255)
cccGTGATATCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTtcc < 5:198396/139‑1 (MQ=255)
cccGTGATATCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTtcc < 2:302637/139‑1 (MQ=255)
cccGTGATATCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTGGCCGAAGCTGCGATATTTTGGCCGCTGAGATCAGTGCTTTTTtcc < 5:11784/139‑1 (MQ=255)
cccGTGATATCAAGATCCTTAAGGCGTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTtcc < 3:134381/139‑1 (MQ=255)
tGATATCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCt > 4:35238/1‑116 (MQ=255)
tGATATCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCt < 3:35238/116‑1 (MQ=255)
tGATATCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGc > 8:2406/1‑139 (MQ=255)
tGATATCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGc > 1:331649/1‑139 (MQ=255)
tGATATCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGc > 1:112670/1‑139 (MQ=255)
tGATATCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGc > 6:66793/1‑139 (MQ=255)
gATATCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCt < 5:253246/139‑1 (MQ=255)
gATATCAAGATCCTTAAGGCTTTTGATCGCCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAg < 4:160686/96‑1 (MQ=255)
gATATCAAGATCCTTAAGGCTTTTGATCGCCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAg > 3:160686/1‑96 (MQ=255)
atttCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTGAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCt < 4:422298/135‑1 (MQ=255)
atatCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATGGAGTTTTTCGTTTTGCCGATGCTGCGTTATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCtg < 5:140245/139‑2 (MQ=255)
atatCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCtc < 3:422123/139‑2 (MQ=255)
atatCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCt < 7:132528/138‑1 (MQ=255)
atatCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCt < 4:66477/138‑1 (MQ=255)
atatCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAAAGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCtc < 6:204147/139‑2 (MQ=255)
aaGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTa < 1:18396/138‑1 (MQ=255)
tAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTg < 8:209914/128‑1 (MQ=255)
tAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTg > 7:209914/1‑128 (MQ=255)
aaGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCAt < 2:112670/138‑1 (MQ=255)
aaGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCAt < 2:415330/138‑1 (MQ=255)
tttGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCATTGCGTc > 5:393025/1‑138 (MQ=255)
ttGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCATTGCGTc > 3:271622/1‑137 (MQ=255)
ttGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCATTGCGTc > 5:259403/1‑137 (MQ=255)
ttGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCATTGCGTCa < 5:66793/138‑1 (MQ=255)
tCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCATTGCGTCAAGGct < 4:271622/139‑1 (MQ=255)
cgtactGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCATTGCGTCAAGGCTCTGGCCAAACGTATcgg > 3:94325/6‑138 (MQ=255)
gtactGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCATTGCGTCAAGGCTCTGGCCAAACGTATcggca < 7:293885/135‑3 (MQ=255)
gtactGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCATTGCGTCAAGGCTCTGGCCAAACGTATcggca < 5:226415/135‑3 (MQ=255)
gtactGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCATTGCGTCAAGGCTCTGGCCAAACGTATcggca < 8:308515/135‑3 (MQ=255)
gtactGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCATTGCGTCAAGGCTCTGGCCAAACGTATcggca < 3:266262/135‑3 (MQ=255)
tGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACa < 4:83430/97‑1 (MQ=255)
tGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACa > 3:83430/1‑97 (MQ=255)
tGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCATTGCGTCAAGGCTCTGGCCAAACGTATCGGCAATGTGAAt > 5:350233/1‑139 (MQ=255)
tGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCATTGCGTCAAGGCTCTGGCCAAACGTATCGGCAATGTGAAt > 6:290236/1‑139 (MQ=255)
gTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGAGGTTTTTTCCCAGCGCTGTACAGTGTCATTGCGTCACCGCTCTGGCCAACCGTATCGGCAAGGTGAAtg > 1:215743/1‑138 (MQ=255)
|
GCTCGATCCGCTGTAATTCTTCCGACACCCATTTTCCGGCAGGCCGGTTACGAAGCGCTAGTAAATCTTTACCCGTGATATCAAGATCCCTAAGGCTTTTGATCGGCAGGTTTTGATAAGTATTTTGAATGTCCTTTAGTTTTTCATCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAAAGAAGTGATTTTTCCCCGGCTTTGTACATTGACATAGTGTCAAGGCTCTGGCCAAACGTATGGGAAATGTGAATC > CP006881/2173809‑2174077
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
