Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A14 F56 I20 R2	1958	92.1	3575798	91.1%	3257551	126.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,174,024	T→C	K301R (AAA→AGA)	U712_10875 ←	CCA‑adding enzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,174,024	0	T	C	100.0%	116.8 / NA	36	K301R (AAA→AGA)	U712_10875	CCA‑adding enzyme
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (16/20); total (16/20)

AAGATCCCTAAGGCTTTTGATCGGCAGGTTTTGATAAGTATTTTGAATGTCCTTTAGTTTTTCATCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAAAGAAGTGATTTTTCCCCGGCTTTGTACATTGACATAGTGTCAAGGCTCTGGCCAAACGTATGGGAAATGTGAATCGCTTCCTTCATCACTTTTCCCGGAAGCTTCCATGCTTTCAGGAAAAGCGGCGCATCTTTCAAATCTATTTCAAGATCAAT  >  CP006881/2173891‑2174157

aaGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTa                                                                                                                                   <  1:18396/138‑1 (MQ=255)

        tAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTg                                                                                                                                     <  8:209914/128‑1 (MQ=255)

        tAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTg                                                                                                                                     >  7:209914/1‑128 (MQ=255)

         aaGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCAt                                                                                                                          <  2:112670/138‑1 (MQ=255)

         aaGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCAt                                                                                                                          <  2:415330/138‑1 (MQ=255)

               tttGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCATTGCGTc                                                                                                                    >  5:393025/1‑138 (MQ=255)

                ttGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCATTGCGTc                                                                                                                    >  3:271622/1‑137 (MQ=255)

                ttGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCATTGCGTc                                                                                                                    >  5:259403/1‑137 (MQ=255)

                ttGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCATTGCGTCa                                                                                                                   <  5:66793/138‑1 (MQ=255)

                    tCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCATTGCGTCAAGGct                                                                                                              <  4:271622/139‑1 (MQ=255)

                                      cgtactGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCATTGCGTCAAGGCTCTGGCCAAACGTATcgg                                                                                             >  3:94325/6‑138 (MQ=255)

                                       gtactGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCATTGCGTCAAGGCTCTGGCCAAACGTATcggca                                                                                           <  8:308515/135‑3 (MQ=255)

                                       gtactGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCATTGCGTCAAGGCTCTGGCCAAACGTATcggca                                                                                           <  7:293885/135‑3 (MQ=255)

                                       gtactGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCATTGCGTCAAGGCTCTGGCCAAACGTATcggca                                                                                           <  3:266262/135‑3 (MQ=255)

                                       gtactGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCATTGCGTCAAGGCTCTGGCCAAACGTATcggca                                                                                           <  5:226415/135‑3 (MQ=255)

                                           tGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACa                                                                                                                                 <  4:83430/97‑1 (MQ=255)

                                           tGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACa                                                                                                                                 >  3:83430/1‑97 (MQ=255)

                                               tGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCATTGCGTCAAGGCTCTGGCCAAACGTATCGGCAATGTGAAt                                                                                   >  5:350233/1‑139 (MQ=255)

                                               tGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCATTGCGTCAAGGCTCTGGCCAAACGTATCGGCAATGTGAAt                                                                                   >  6:290236/1‑139 (MQ=255)

                                                gTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGAGGTTTTTTCCCAGCGCTGTACAGTGTCATTGCGTCACCGCTCTGGCCAACCGTATCGGCAAGGTGAAtg                                                                                  >  1:215743/1‑138 (MQ=255)

                                                            cttttcatcCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCATTGCGTCAAGGCTCTGGCCAAACGTATCGGCAATGTGAATGGCTTCCTtgat                                                                       <  7:2406/134‑1 (MQ=255)

                                                             ttttcatcCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCATTGCGTCAAGGCTCTGGCCAAACGTATCGGCAATGTGAATGGCTTCCTtgatca                                                                     <  6:259403/136‑1 (MQ=255)

                                                                                 ttGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCATTGCGTCAAGGCTCTGGCCa                                                                                                       <  4:412150/85‑1 (MQ=255)

                                                                                 ttGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCATTGCGTCAAGGCTCTGGCCa                                                                                                       >  3:412150/1‑85 (MQ=255)

                                                                                  tGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCAt                                                                                                                          >  5:1304/1‑65 (MQ=255)

                                                                                  tGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCAt                                                                                                                          <  6:1304/65‑1 (MQ=255)

                                                                                       aaGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCATTGCGTCAAGGCTCTGGCCAAACGTATCGGCAATGTGAATGGCTTCCTTGATCACTTTTCCCGGGAGCTTCCAGGCTTTc                                           <  2:331649/139‑1 (MQ=255)

                                                                                             cGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCATTGCGTCAAGGCTCTGGCCAAACGTATCGGCAATGTGAATGGCTTCCTTGATCACTTTTCCCGGGAGCTTCCAGGCTTTCAGGaa                                      >  1:359579/1‑138 (MQ=255)

                                                                                                       gCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCATTGCGTCAAGGCTCTGGCCAAACGTATCGGCAATGTGAATGGCTTCCTTGATCACTTTTCCCGGGAGCTTCCAGGCTTTCAGGAAAAGGGgcgcgt                           >  1:42345/1‑137 (MQ=255)

                                                                                                           cTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCATTGCGTCAAGGCTCTGGCCAAACGTATCGGCAATGTGAATGGCTTCCTTGATCACTTTTCCCGGGAGCTTCCAGGCTTTCAGGAAAAGGGGCGCGTCttt                       <  2:42345/139‑1 (MQ=255)

                                                                                                            tGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCATTGCGTCAAGGCTCTGGCCAAACGTATCGGCAATGTGAATGGCTTCCTTGATCACTTTTCCCGGGAGCTTCCAGGCTTTCAGGAAAAGGGGCGCGTCTTTc                      >  3:440333/1‑139 (MQ=255)

                                                                                                                     tgcttttttCCCAGCTCTGTACATTGTCATTGCGTCAAGGCTCTGGCCAAACGTATCGGCAATGTGAATGGCTTCCTTGATCACTTTTCCCGGGAGCTTCCAGGCTTTCAGGAAAAGGGGCGCGTCTTTCAAAACTAtg             >  4:305824/4‑138 (MQ=255)

                                                                                                                       cttttttCCCAGCTCTGTACATTGTCATTGCGTCAAGGCTCTGGCCAAACGTATCGGCAATGTGAATGGCTTCCTTGATCACTTTTCCCGGGAGCTTCCAGGCTTTCAGGAAAAGGGGCGCGTCTTTCAAAACTAtgcc           <  3:305824/138‑4 (MQ=255)

                                                                                                                       cgattttCCCAGCTCTGTACATTGTCATTGCGTCAAGGCTCTGGCCAAACGTATCGGCAATGTGAATGGCTTCCTTGATCACTTTTCCCGGGAGCTTCCAGGCTTTCAGGAAAAGGGGCGCGTCTTTCAAAACTAtgcc           <  8:197962/136‑4 (MQ=255)

                                                                                                                            ttcccAGCTCTGTACATTGTCATTGCGTCAAGGCTCTGGCCAAACGTATAGTCAATGTGAATGGCTTCCTTGATCACTTTTCCCGGGAGCTTCCAGGCTTTCAGGAAAAGGGGCGCGTCTTTCAAAACTATGCCAAgg       <  4:179756/136‑2 (MQ=255)

                                                                                                                                 agCTCTGTACATTGTCATTGCGTCAAGGCTCTGGCCAAACGTATCGGCAATGTGAATGGCTTCCTTGATCACTTTTCCCGGGAGCTTCCAGGCTTTCAGGAAAAGGGGCGCGTCTTTCAAAACTATGCCAAGGTTAAt  >  3:386818/2‑138 (MQ=255)

AAGATCCCTAAGGCTTTTGATCGGCAGGTTTTGATAAGTATTTTGAATGTCCTTTAGTTTTTCATCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAAAGAAGTGATTTTTCCCCGGCTTTGTACATTGACATAGTGTCAAGGCTCTGGCCAAACGTATGGGAAATGTGAATCGCTTCCTTCATCACTTTTCCCGGAAGCTTCCATGCTTTCAGGAAAAGCGGCGCATCTTTCAAATCTATTTCAAGATCAAT  >  CP006881/2173891‑2174157

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A