Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A14 F56 I20 R2
|
1958 |
92.1 |
3575798 |
91.1% |
3257551 |
126.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
CP006881 |
2,196,023 |
C→T |
D315N (GAC→AAC) |
U712_11005 ← |
3‑dehydroquinate synthase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | CP006881 | 2,196,023 | 0 | C | T | 98.5%
| 202.9
/ NA
| 66 | D315N (GAC→AAC) | U712_11005 | 3‑dehydroquinate synthase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (33/32); minor base G (0/1); total (33/33) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CTCCAATCGCCATTTGTTCAGCCAGCTCTCAAGTTCATTTCTGGAAAATGCGTGATCAGCAACTTTTCCTAATTCGTTGAGCACGATAAACTGAATCTTCCCGCCGCGGGTTTTCTTATCATTCATCATACGGTCCAATAGAACCGAAGTTTCCGTCTCATTTTTAATTTGACTTGGATAGCCCAGGCTTATTAGCCAACTGACCAAACGTTTCCTGTCCATTTCACAGCCCGCTGTCTTTTCGCTTATATATAAAGC > CP006881/2195889‑2196146
|
ctccAATCGCCATTTGTTCAGCCAGCTCTCAAGTTCATTTCTGGAAAATGTATGATCAGCAACTTTCCCTAATTCGTTGAGCACAATAAACTGAATCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCaa < 2:302167/138‑1 (MQ=255)
aTCGCCATTTGTTCAGCCAGCTCTCAAGTTCATTTCACGAAAAAGTATGATCAGCAACTTTCCCTAATTCGTTGAGCACAATAAACTGAAACAACACGCCACCGGAGTTCTAATCATTAATCATACGGTTCAAGAGAAc > 2:135035/1‑139 (MQ=255)
tCGCCATTTGTTCAGCCAGCTCTCAAGTTCATTTCTGGAAAATGTATGATCAGCAACTTTCCCTAATTCGTTGAGCACAATAAACTGAATCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAAcc > 3:360337/1‑139 (MQ=255)
cATTTGTTCAGCCAGCTCTCAAGTTCATTTCTGGAAAATGTATGATCAGCAACTTTCCCTAATTCGTTGAGCACAATAACCTGAATCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAg > 7:2848/1‑139 (MQ=255)
gTTCAGCCAGCTCTCAAGTTCATTTCTGGAAAATGTATGATCAGCAACTTTCCCTAATTCGTTGAGCACAATAAACTGAATCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTc < 4:103969/138‑1 (MQ=255)
ttCAGCCAGCTCTCAAGTTCATTTCTGGAAAATGTATGATCAGCAACTTTCCCTAATTCGTTGAGCACAATAAACTGAATCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCg < 6:38809/139‑1 (MQ=255)
cagccagcTATCAAGTTCATTTCTGGAAAATGTATGATCAGCAACTTTCCCTAATTCGTTGAGCAGAATAAACTGAATCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGt < 8:23718/138‑1 (MQ=255)
cAAGTTCATTTCTGGAAAATGTATGATCAGCAACTTTCCCTAATTCGTTGAGCACAATAAACTGAATCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCttttctgat < 4:28854/139‑6 (MQ=255)
cATTTCTGGAAAATGTATGATCAGCAACTTTCCCTAATTCGTTGAGCACAATAAACTGAATCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGAc < 8:37723/138‑1 (MQ=255)
ttCTGGAAAATGTATGATCAGCAACTTTCCCTAATTCGTTGAGCACAATAAACTGAATCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTgg > 7:239548/1‑139 (MQ=255)
ttCTGGAAAATGTATGATCAGCAACTTTCCCTAATTCGTTGAGCACAATAAACTGAATCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTg > 7:176453/1‑138 (MQ=255)
cTGGAAAATGTATGATCAGCAACTTTCCCTAATTCGTTGAGCACAATAAACTGAATCTTCCCGCCACGTGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGa < 6:410145/138‑1 (MQ=255)
cTGGAAAATGTATGATCAGCAACTTTCCCTAATTCGTTGAGCACAATAAACTGAATCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGa < 4:360337/138‑1 (MQ=255)
cTGGAAAATGTATGATCAGCAACTTTCCCTAATTCGTTGAGCACAATAAACTGAATCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGa < 6:84581/138‑1 (MQ=255)
atgtatgatgaGCAACTTTCGCTAATTCTGTGAGCACATTAAACTGAATCTTCCCGCCACGGGTTTTCTTATCATTCAGCATACGGTTCAAGAGAAGCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATATCCCAg < 2:313797/134‑1 (MQ=255)
atgtatGATCAGCAACTTTCCCTAATTCGTTGAGCACAATAAACTGAATCTTCCCGCCACGGGTTTTCTTATCATTCCTCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAg < 3:19893/134‑1 (MQ=255)
atgtatGATAAGCAACTTTCCCTAATTCGTTGAGCACAATAAACTGAATCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGATCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAg < 5:431359/134‑1 (MQ=255)
tgtatGATCAGCAACTTTCCCTAATTCGTTGAGCACAATAAACTGAATCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAgg > 8:28423/5‑139 (MQ=255)
tCAGCAACTTTCCCTAATTCGTTGAGCACAATAAACTGAATCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCttttta < 8:2848/139‑1 (MQ=255)
cAACTTTCCCTAATTCGTTGAGCACAATAAACTGAATCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCGTTTTAGCCa > 3:53272/1‑139 (MQ=255)
aTTCGTTGAGCACAATAAACTGAATCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAAc < 7:34876/138‑1 (MQ=255)
ttCGTTGAGCACAATAAACTGAATCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGt > 7:375647/1‑139 (MQ=255)
ttCGTTGAGCACAATAAACTGAATCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCGTTTCTGATTTGACGTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGt > 6:900/1‑139 (MQ=255)
tGAGCACAATAAACTGAATCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCt > 8:429580/1‑139 (MQ=255)
gAGCACAATAAACTGAATCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCt < 3:60894/138‑1 (MQ=255)
cacaATAAACTGAATCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGAATTGGATAACACAGGCGTTTTAGCCAGCTGACCAAACGTTTTCTGTc > 4:89703/1‑138 (MQ=255)
cacaATAAACTGAATCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCCGGTTTCGGATTTGACATGGATAACCCCGGCGTTTTAGGCAGCTGACCAAACGTTTTATGTc > 6:66415/1‑138 (MQ=255)
tAAACTGAATCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGtttt > 5:204472/1‑127 (MQ=255)
tAAACTGAATCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGtttt < 6:204472/128‑2 (MQ=255)
aaCTGAATCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTcac < 6:410882/139‑1 (MQ=255)
aaCTGAATCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTca < 8:239548/138‑1 (MQ=255)
aCTGAATCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTca > 7:56049/1‑137 (MQ=255)
aTCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTGTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGCCCAAACGTTTTCTGTCCATTTCACAGccta > 7:417312/1‑137 (MQ=255)
aTCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAg > 8:148010/1‑92 (MQ=255)
aTCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAg < 7:148010/92‑1 (MQ=255)
aTCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTTTCCATTTCACAGccta > 2:181963/1‑137 (MQ=255)
aTCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTCACAGccta > 4:93545/1‑137 (MQ=255)
aTCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTCACAGccta > 8:26394/1‑137 (MQ=255)
aTCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTCACAGccta > 8:368071/1‑137 (MQ=255)
aTCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTCACAGccaa > 2:231931/1‑137 (MQ=255)
aTCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCGTTTTCGCCAGATGCCCAAACGTTTTCTGTCCATTTCACAGcctc > 4:198445/1‑137 (MQ=255)
aTCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTGTTCTGATTTTAGTTGGATTACCCCGGCTTTTTTGCCAGCTGACCAAACGTTTTCTGTCCATTTAACAGccta > 1:143465/1‑137 (MQ=255)
aTCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTACGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTCACAggcta < 1:135035/139‑5 (MQ=255)
tCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTCACAGcctacagt > 3:129595/1‑133 (MQ=255)
tCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTCACAGcctacagt > 7:65395/1‑133 (MQ=255)
cccGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCCAACGTTTTCTGTCCATTTCACAGcctac < 6:343478/135‑4 (MQ=255)
ccacgGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTCACAGCCTACAGTCtttt < 6:252034/136‑1 (MQ=255)
ccacgGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTCACAGCCTACAGTCtttt < 1:224719/136‑1 (MQ=255)
ccacgGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTCACAGCCTACAGTCtttt < 8:176453/136‑1 (MQ=255)
ccacgGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTCACAGCCTACAGTCtttt < 4:153830/136‑1 (MQ=255)
ccacgGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTCACAGCCTACAGTCtttt < 4:53272/136‑1 (MQ=255)
ccacgGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTCACAGCCTACAGTCtttt < 5:174154/136‑1 (MQ=255)
gTTTTCTTATCATTCATCATACGGTGCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTCACAGCCTACAGTCTTTTCGCtt < 5:423230/138‑1 (MQ=255)
ttCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTca > 4:363709/1‑114 (MQ=255)
ttCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTca < 3:363709/114‑1 (MQ=255)
ttCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCGTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTAACAGCCTACACTCTTTTAGCTtata > 4:2036/1‑138 (MQ=255)
aTCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTCACAGCCTACAGTCTTTTCGCTtatatat < 4:395418/136‑1 (MQ=255)
cattcatCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTca < 4:397441/107‑1 (MQ=255)
cattcatCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTca > 3:397441/1‑107 (MQ=255)
cattcatCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTCACAGCCTACAGTCTTTTCGCTTAAATATAAAg > 7:7070/1‑138 (MQ=255)
cattcatCATACGGTTCAAGAGAACCGAAGTTTCCGTCGCTTTTATGATTTGACTTGGATAACCCACGCTTTTTAGCCAGCGGACCAAACGTTTTCTGTCCATTTGACATCCTACAGTCTTTTAGCGTTTATATaaat > 2:195551/1‑137 (MQ=255)
attcatCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTCACAGCCTACAGTCTTTTCGCTTATATATAAAGc > 1:43975/1‑138 (MQ=255)
atcatACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGAc < 7:407578/80‑1 (MQ=255)
atcatACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGAc > 8:407578/1‑80 (MQ=255)
aCGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTCACAGCCTACAGTCtttt > 4:111418/1‑113 (MQ=255)
aCGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTCACAGCCTACAGTCtttt < 3:111418/113‑1 (MQ=255)
|
CTCCAATCGCCATTTGTTCAGCCAGCTCTCAAGTTCATTTCTGGAAAATGCGTGATCAGCAACTTTTCCTAATTCGTTGAGCACGATAAACTGAATCTTCCCGCCGCGGGTTTTCTTATCATTCATCATACGGTCCAATAGAACCGAAGTTTCCGTCTCATTTTTAATTTGACTTGGATAGCCCAGGCTTATTAGCCAACTGACCAAACGTTTCCTGTCCATTTCACAGCCCGCTGTCTTTTCGCTTATATATAAAGC > CP006881/2195889‑2196146
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
