Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A14 F56 I20 R2 1958 92.1 3575798 91.1% 3257551 126.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 2,196,234 G→A F244F (TTC→TTT)  U712_11005 ← 3‑dehydroquinate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,196,2340GA99.1% 334.5 / NA 106F244F (TTC→TTT) U712_110053‑dehydroquinate synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (61/44);  minor base T (0/1);  total (61/45)
Fisher's exact test for biased strand distribution p-value = 4.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 106 total aligned reads displayed.

TCCTGTCCATTTCACAGCCCGCTGTCTTTTCGCTTATATATAAAGCAAACTGCATCCCGAGGGCTACGGCATCACCGTGAGTGATCTGTCCGTATCCATATTCAGCCTCAACGGCATGGCCGAGCGTATGTCCGAAGTTTAAGTAAGCTCTGATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGATGCTTTTATTGAAATCCCTTTAAAAATCATGTCATTCAGCTGATCACTGGTGATATCACGCAGTGAGCGGATAT  >  CP006881/2196101‑2196361
                                                                                                                                     |                                                                                                                               
ttcTGTCCATTTCACAGCCTACAGTCTTTTCGCTTATATATAAAGCAAACTGCATTCCGAGGGCTACGGCATCACCGTGAGTGATCTGCCCATATCCATATTCCGCCTCAACGCCATGGCCGAGCGTATGCCCAAAGtt                                                                                                                            >  6:113433/3‑139 (MQ=255)
     tCCATTTCACAGCCTACAGTCTTTTCGCTTATATATAAAGCAAACTGCATTCCGAGGGCTACGGCATCACCGTGAGTGATCTGCCCATATCCATATTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTtaag                                                                                                                        >  3:413560/1‑138 (MQ=255)
       cATTTCACAGCCTACAGTCTTTTCGCTTATATATAAAGCAAACTGCATTCCGAGGGCTACGGCATCACCGTGAGTGATCTGCCCATATCCATATTCCGCCTCAACGGCATTGCCGAGCGTATGCCCAAAGTTtaagta                                                                                                                      <  7:250403/138‑1 (MQ=255)
       cATTTCACAGCCTACAGTCTTTTCGCTTATATATAAAGCAAACTGCATTCCGAGGGCTACGGCATCACCGTGAGTGATCTGCCCATATCCATATTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTtaagtaa                                                                                                                     <  7:28423/139‑1 (MQ=255)
        aTTTCACAGCCTACAGTCTTTTCGCTTATATATAAAGCAAACTGCATTCCGAGGGCTACGGCATCACCGTGAGTGATCTGCCCATATCCATATTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTtaagtaag                                                                                                                    <  5:258970/139‑1 (MQ=255)
                 cctacagTCTTTTCGCTTATATATAAAGCAAACTGCATTACGAGGGCTACGGCATCACCGTGAGTGATCTGCCCATATCCATATTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATcc                                                                                                           >  7:373918/7‑139 (MQ=255)
                  ctacagTCTTTTCGCTTATATATAAAGCTATCTGCATTCCGAGGGCTACGGCATCACCGTGAGTGATCTGCCCATATCCAAATTCCGCATCAACGGCATGGCCGAGCGTATGCCCAAATTTTAAGTAAGCTCTTATccc                                                                                                          <  5:412346/134‑1 (MQ=255)
                    acagTCTTTTCGCTTATATATAAAGCAAACTGCATTCCGAGGGCTACGGCATCACCGTGAGTGATCTGCCCATATCCATATTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCct                                                                                                        >  3:8185/4‑139 (MQ=255)
                      agTCTTTTCGCTTATATATAAAGCAAACTGCATTCCGAGGGCTACGGCATCACCGTGAGTGATCTGCCCATATCCATATTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCctct                                                                                                      >  6:165591/2‑139 (MQ=255)
                       gTCTTTTCGCTTATATATAAAGCAAACTGCATTCCGAGGGCTACGGCATCACCGTGAGTGATCTGCCCATATCCATATTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCtctt                                                                                                     <  3:162666/139‑1 (MQ=255)
                          ttttCGCTTATATATAAAGCAAACTGCATTCCGAGGGCTACGGCATCACCGTGAGTGATCTGCCCATATCCATATTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTcttctt                                                                                                  >  4:93122/1‑139 (MQ=255)
                           tttCGCTTATATATAAAGCATACTGCATTCCGCGGGCTACGGCATCACCGTGAGTGATCTGCCCATATCCATATTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCttt                                                                                                 >  4:226689/1‑139 (MQ=255)
                           tttCGCTTATATATAAAGCAACCTGCATTCCGAGGGCTACGGCATCACCGTGAGTGATCTGCCCATATCCCTATTCCGCCTCACCGGCATGGCCGCGCGTATGCCCACAGGTTACGTACGCTCTTAGCCCCTCGTCttt                                                                                                 >  4:324714/1‑139 (MQ=255)
                           tttCGCTTATATATAAAGCAAACTGCATTCCGAGGGCTACGGCATCACCGTGTGTGATCTGCCCATATCCATATTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCttt                                                                                                 >  5:93915/1‑139 (MQ=255)
                           tttCGCTTATATATAAAGCAAACTGCATTCCGAGGGCTACGGCATCACCGTGAGTGATCTGCCCATATCCATATTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGATTAAGTAAGATCTTATACCCTCTTAttt                                                                                                 >  5:317546/1‑139 (MQ=255)
                             tCGCTTATATATAAAGCAAACTGCATTCCGAGGGCTACGGCATCACCGTGAGTGATCTGCCCATATCCATATTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCttttt                                                                                               >  5:364601/1‑139 (MQ=255)
                             tCGCTTATATATAAAGCAAACTGAATTCCGAGGGCTACGGCATCACAGTGAGAGATCTGCACATATCCACATTACGCCACAACGGCATGGCCGAGCGTATGCGCAAAGTGTAAGAAAGCTCTTAACCCCTCTTCtgttt                                                                                               >  6:412346/1‑139 (MQ=255)
                               gCTTATATATAAAGCAAACTGCATTCCGAGGGCTACGGCATCACCGTGAGTGATCTGCCCATATCCATATTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCa                                                                                             <  4:129595/139‑1 (MQ=255)
                                    tatataAAGCAAACTGCATTCCGAGGGCTACGGCATCACCGTGAGTGATCTGCCCATATCCATATTCCGCCTCAACGTCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCctg                                                                                        <  8:417312/139‑1 (MQ=255)
                                    tatataAAGCAAACTGCATTCCGAGGGCTACGGCATCACCGTGAGTGATCTGCCCATATCCATATTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCctg                                                                                        >  7:184616/1‑139 (MQ=255)
                                          aaGCAAACTGCATTCCGAGGGCTACGGCATCACCGTGAGTGATCTGCCCATATCCATATTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAAc                                                                                  <  3:390644/139‑1 (MQ=255)
                                          aaGCAAACTGCATTCCGAGGGCTACGGCATCACCGTGAGTGATCTGCCCATATCCATATTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAAc                                                                                  <  7:250270/139‑1 (MQ=255)
                                           aGCAAACTGCATTCCGAGGGCTACGGCATCACCGTGAGTGCTCTGCCCATATCCATATTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAAc                                                                                  >  2:147245/1‑138 (MQ=255)
                                           aGCAAACTGCATTCCGAGGGCTACGGCATCACCGTGAGTGATCTGCCCATATCCATATTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTAATCCTGCTGAACg                                                                                 >  7:420745/1‑139 (MQ=255)
                                                aCTGCATTCCGAGGGCTACGGCATCACCGTGAGTGATCTGCCCATATCCATATTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGa                                                                            >  4:170242/1‑139 (MQ=255)
                                                aCTGCATTCCGAGGGCTACGGCATCACCGTGAGTGATCTGCCCATATCCATATTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGa                                                                            <  3:110314/139‑1 (MQ=255)
                                                aCTGCATTCCGAGGGCTACGGCATCACCGTGAGTGATCTGCCCATATCCATATTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGa                                                                            <  3:198445/139‑1 (MQ=255)
                                                aCTGCATTCCGAGGGCTACGGCATCACCGTGAGTGATCTGCCCATATCCATATTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGa                                                                            <  1:231931/139‑1 (MQ=255)
                                                      ttccGAGGGCTACGGCATCACCGTGAGTGATCTGCCCATATCCATATTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCtt                                                                       >  3:71540/3‑138 (MQ=255)
                                                       tccGAGGGCTACGGCATCACCGTGAGTGATCTGCCCATATCCATATTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATACCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCttt                                                                      >  6:255989/2‑138 (MQ=255)
                                                              gCTACGGCATCACCGTGAGTGATCTGCCCATATCCATATTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTtaagt                                                                                                                       <  4:51928/82‑1 (MQ=255)
                                                              gCTACGGCATCACCGTGAGTGATCTGCCCATATCCATATTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGaaa                                                              >  7:228746/1‑139 (MQ=255)
                                                              gCTACGGCATCACCGTGAGTGATCTGCCCATATCCATATTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGaaa                                                              >  2:63634/1‑139 (MQ=255)
                                                                   ggCATCACCGTGAGTGATCTGCCCATATCCATATTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCt                                                         >  7:376562/1‑139 (MQ=255)
                                                                        cACCGTGAGTGATCTGCCCATATCCATATTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTtaagta                                                                                                                      >  2:88422/1‑73 (MQ=255)
                                                                        cACCGTGAGTGATCTGCCCATATCCATATTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTtaagta                                                                                                                      <  1:88422/73‑1 (MQ=255)
                                                                        cACCGTGAGTGATCTGCCCATATCCATATTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGCACGACAGCCGCTTTAATTTATATGCCTTTaa                                                     >  8:143734/1‑138 (MQ=255)
                                                                             tgagtgaTCTGCCCATATCCATATTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAAtaa                                               >  5:365551/1‑137 (MQ=255)
                                                                               agtgaTCTGCCCATATCCATATTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACAACAGACGCTTTAATTGAAATGCCTTTAAAAATCATg                                             >  5:34387/1‑139 (MQ=255)
                                                                                   aTCTGCCCATATCCATATTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTtaagta                                                                                                                      >  8:230762/1‑62 (MQ=255)
                                                                                   aTCTGCCCATATCCATATTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTtaagta                                                                                                                      <  7:230762/62‑1 (MQ=255)
                                                                                   aTCTGCCCATATCCATATTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCa                                          <  8:413273/138‑1 (MQ=255)
                                                                                   aTCTGCCCATATCCATATTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCAt                                         >  7:78506/1‑139 (MQ=255)
                                                                                       gcccatATCCATATTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGa                                                                            >  1:172247/6‑100 (MQ=255)
                                                                                       gcccatATCCATATTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGa                                                                            <  2:172247/95‑1 (MQ=255)
                                                                                        cccatATCCATATTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAg                                     <  3:93122/134‑1 (MQ=255)
                                                                                        cccatATCCATATTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGc                                    >  2:418706/5‑139 (MQ=255)
                                                                                        cccatATCCATATTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGc                                    >  1:282568/5‑139 (MQ=255)
                                                                                         ccatATCCATATTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCt                                   <  4:411308/136‑1 (MQ=255)
                                                                                         ccatATCCATATTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACAACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCt                                   <  6:34387/136‑1 (MQ=255)
                                                                                             aTCCATATTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCGCCTCTTCTTTTTCATCCTGCTGAACGACAGCCGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGAt                                >  2:155432/1‑138 (MQ=255)
                                                                                             aTCCATATTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACa                                                                              <  5:284012/92‑1 (MQ=255)
                                                                                             aTCCATATTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACa                                                                              >  6:284012/1‑92 (MQ=255)
                                                                                             aTCCATATTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGAt                                >  6:398947/1‑138 (MQ=255)
                                                                                              tCCATATTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATc                                                                                                            <  3:49/61‑1 (MQ=255)
                                                                                              tCCATATTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATc                                                                                                            >  4:49/1‑61 (MQ=255)
                                                                                                cATATTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCat                             <  4:413560/138‑2 (MQ=255)
                                                                                                  tatTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGtt                                                                                                                            <  1:292019/41‑1 (MQ=255)
                                                                                                  tatTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCattag                          >  3:238662/1‑135 (MQ=255)
                                                                                                  tatTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCattag                          <  3:226689/139‑5 (MQ=255)
                                                                                                  tatTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCattag                          <  1:147245/139‑5 (MQ=255)
                                                                                                  tatTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCAAGTCATTCAGCTGATCattag                          >  7:102934/1‑135 (MQ=255)
                                                                                                  tatTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCATTTAAAAATCATGTCATTCAGCTGATCattag                          >  2:136297/1‑135 (MQ=255)
                                                                                                  tatTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCAACTCTTCTTTTTCATCCTGCTGAACGACAGACGATTTAATTGAAATGCATTTAAAAATCATTTCATTCAGCTGATCattag                          >  6:30939/1‑135 (MQ=255)
                                                                                                    ttCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTg                        <  5:173907/139‑1 (MQ=255)
                                                                                                    ttCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTg                        <  3:85729/139‑1 (MQ=255)
                                                                                                     tccgCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTGa                       >  1:301485/4‑139 (MQ=255)
                                                                                                       cgCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTGata                     >  3:107397/2‑139 (MQ=255)
                                                                                                       cgCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTGata                     >  5:167524/2‑139 (MQ=255)
                                                                                                       cgCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTGata                     >  8:405359/2‑139 (MQ=255)
                                                                                                        gCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTGata                     >  7:398478/1‑138 (MQ=255)
                                                                                                         ccTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCttt                                                                      <  8:190802/88‑1 (MQ=255)
                                                                                                         ccTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCttt                                                                      >  7:190802/1‑88 (MQ=255)
                                                                                                         ccTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTGatat                    >  1:342056/1‑138 (MQ=255)
                                                                                                         ccTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTGatat                    >  2:265732/1‑138 (MQ=255)
                                                                                                         ccTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTGatat                    >  7:169803/1‑138 (MQ=255)
                                                                                                          cTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTGATATCa                  <  8:56049/139‑1 (MQ=255)
                                                                                                             aaCGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTGATATCACg                >  4:277398/1‑138 (MQ=255)
                                                                                                             aaCGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAAAGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATTTCATTAAGCTGATCATTAGTGATATCACg                <  7:160367/138‑1 (MQ=255)
                                                                                                              aCGGGATGGCCGAGCGTATGCCCAAAGTTTTACTAAGCTCTTATCCCCTGTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTGATATCACGCa              <  5:113433/139‑1 (MQ=255)
                                                                                                              aCGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTGATATCACGCa              <  2:143465/139‑1 (MQ=255)
                                                                                                              aCGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTGATATCACGCa              <  4:238662/139‑1 (MQ=255)
                                                                                                              aCGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTGATATCACGCa              <  8:228746/139‑1 (MQ=255)
                                                                                                                 gCATGGCCGAGCGTATGCCCTAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTGATATCACGCAGTg           <  6:317546/139‑1 (MQ=255)
                                                                                                                 gCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTGatat                    <  6:422708/130‑1 (MQ=255)
                                                                                                                 gCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTGatat                    >  5:422708/1‑130 (MQ=255)
                                                                                                                 gCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTGATATCACGCAGTg           <  4:228328/139‑1 (MQ=255)
                                                                                                                    tGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTGATATCACGCAGTGAgt        <  2:301485/139‑2 (MQ=255)
                                                                                                                    tGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTGATATCACGCAGTGAgt        <  1:265732/139‑2 (MQ=255)
                                                                                                                    tGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTGATATCACGCAGTGAgt        <  5:398947/139‑2 (MQ=255)
                                                                                                                    tGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTGATATCACGCAGTGAgt        >  3:222563/1‑138 (MQ=255)
                                                                                                                    tGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTGATATCACGCAGTGAgt        <  2:342056/139‑2 (MQ=255)
                                                                                                                    tGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCATTAAAAATCATGTCATTCAGCTGATCATTAGTGATATCACGCAGTGAgt        >  8:159267/1‑138 (MQ=255)
                                                                                                                       ccGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGaa                                                                                   <  1:401725/61‑1 (MQ=255)
                                                                                                                       ccGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGaa                                                                                   >  2:401725/1‑61 (MQ=255)
                                                                                                                          aGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACCGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCCGCTGATCATTAGTGATATCACGCAGTGAGTGGatat  >  8:260560/1‑139 (MQ=255)
                                                                                                                          aGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCa                                          <  3:314682/99‑1 (MQ=255)
                                                                                                                          aGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCa                                          >  4:314682/1‑99 (MQ=255)
                                                                                                                          aGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTGATATCACGCAGTGAGTGGatat  >  1:359369/1‑139 (MQ=255)
                                                                                                                          aGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTGTAAAAATCATGTCATTCAGCTGATCATTAGTGATATAACGCAGTGAGTGGatat  >  6:161377/1‑139 (MQ=255)
                                                                                                                                     |                                                                                                                               
TCCTGTCCATTTCACAGCCCGCTGTCTTTTCGCTTATATATAAAGCAAACTGCATCCCGAGGGCTACGGCATCACCGTGAGTGATCTGTCCGTATCCATATTCAGCCTCAACGGCATGGCCGAGCGTATGTCCGAAGTTTAAGTAAGCTCTGATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGATGCTTTTATTGAAATCCCTTTAAAAATCATGTCATTCAGCTGATCACTGGTGATATCACGCAGTGAGCGGATAT  >  CP006881/2196101‑2196361

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A