Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A14 F56 I20 R2	1958	92.1	3575798	91.1%	3257551	126.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,197,031	G→T	G369G (GGC→GGA)	U712_11010 ←	Chorismate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,197,031	0	G	T	98.4%	186.8 / NA	65	G369G (GGC→GGA)	U712_11010	Chorismate synthase
Reads supporting (aligned to +/- strand): ref base G (0/1); new base T (30/33); total (30/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTTCTGATGCCTTGTCCGATAAAAACAGGATACGACGAGGAAGCGGTTTGAACATGCAGTGTTTTCATTAAAATTCCCTCGACAGTTTTCTCATATTCTCCACATTTTCTCTAATGCGGTCGATTTGATCTAAGCCGAATTGTTCAACGACTGCGTTCGCAATCTCCCATGCGACAGCCGCTTCAGCTACTACACTTGCCGCAGGCACTGCACAGCTGTCTGAACGTTCAATGCTGGCAGAAAACGGTTCTTTCGTTTCAAT  >  CP006881/2196897‑2197159

ttttCTGATACCTTGTCCGATAAAAACAGGATATGACGAGGAAGCAGTTTGAACATGCAGTGTCTTCATTAAAATTCCCTCGACAGTTTTCTCATATTCTCCACATTTTCTCTAATGCGGTCAATTTGATCTAATCCg                                                                                                                               <  4:256387/138‑1 (MQ=255)

  ttCTGATACCTTGTCCGATAAAAACAGGATATGACGAGGAAGCAGTTTGAACATGCAGTGTCTTCATTAAAATTCCCTCGACAGTTTTCTCATATTCTCCACATTTTCTCTAATGCGGTCAATTTGATCTAATCCGaa                                                                                                                             <  5:416263/138‑1 (MQ=255)

  ttCTGATACCTTGTCCGATAAAAACAGGATATGACGAGGAAGCAGTTTGAACATGCAGTGTCTTCATTAAAATTCCCTCGACAGTTTTCTCATATTCTCCACATTTTCTCTAATGCGGTCAATTTGATCTAATCCGaa                                                                                                                             <  3:118271/138‑1 (MQ=255)

       ataccTTGTCCGATAAAAACAGGATATGAGGAGGAAGCAGTTTGAACATGCTGTGTCTTCATAAAAATTCCCTCGACATTTTTCTCATATTCTCCACATTTTCTCTAATGCGGTCAATTTGTTCTATTCCGAATTg                                                                                                                          <  2:329722/133‑1 (MQ=255)

       ataccTTGTCCGATAAAAACAGGATATGACGAGGAAGCAGTTTGAACATGCAGTGTCTTCATTAAAATTCCCTCGACAGTTTTCTCATATTCTCCACATTTTCTCTAATGCGGTCAATTTGATCTAATCCGAATTGtt                                                                                                                        >  7:429613/4‑138 (MQ=255)

       ataccTTGTCCGATAAAAACAGGATATGACGAGGAAGCAGTTTGAACATGCAGTGTCTTCATTAAAATTCCCTCGACAGTTTTCTCATATTCTCCACATTTTCTCTAATGCGGTCAATTTGATCTAATCCGAATTGtt                                                                                                                        >  4:155135/4‑138 (MQ=255)

       ataccTTGTCCGATAAAAACAGGATATGACGAGGAAGCAGTTTGAACATGCAGTGTCTTCATTAAAATTCCCTCGACAGTTTTCTCATATTCTCCACATTTTCTCTAATGCGGTCAATTTGATCTAATCCGAATTGtt                                                                                                                        >  4:150614/4‑138 (MQ=255)

             tGTCCGATAAAAACAGGATATGACGAGGAAGCAGTTTGAACATGCAGTGTCTTCATTAAAATTCCCTCGACAGTTTTCTCATATTCTCCACATTTTCTCTAATGCGGTCAATTTGATCTAATCCGAATTg                                                                                                                          <  6:40671/130‑1 (MQ=255)

             tGTCCGATAAAAACAGGATATGACGAGGAAGCAGTTTGAACATGCAGTGTCTTCATTAAAATTCCCTCGACAGTTTTCTCATATTCTCCACATTTTCTCTAATGCGGTCAATTTGATCTAATCCGAATTg                                                                                                                          >  5:40671/1‑130 (MQ=255)

              gTCCTATAAAAACAGTATATGACGAGGAAGCTGTTTGAACATGCAGTGTCTTCATTAAAATTCACTCGACAGTTTTCTCATATTCTCCACATTTTCTCTAATGCGGTCAATTTGATCTATTCCGATTTGTTCAACGAc                                                                                                                 <  1:142642/138‑1 (MQ=255)

               tCCGATAAAAACAGGATATGACGAGGAAGCAGTTTGAACATGCAGTGTCTTCATTAAAATTCCCTCGACAGTTTTCTCATATTCTCCACATTTTCTCTAATGCGGTCAATTTGATCTAATCCGAATTGTTCAACGACTg                                                                                                               >  1:88981/1‑139 (MQ=255)

               tCCGATAAAAACAGGATATGACGAGGAAGCAGTTTGAACATGCAGTGTCTTCATTAAAATTCCCTCGACAGTTTTCTCATATTCTCCACATTTTCTCTAATGCGGTCAATTTGATCTAATCCGAATTGTTCAACGACTg                                                                                                               >  2:160536/1‑139 (MQ=255)

               tCCGATAAAAACAGGATATGACGAGGAAGCAGTTTGAACATGCAGTGTCTTCATTAAAATTCCCTCGACAGTTTTCTCATATTCTCCACATTTTCTCTAATGCGGTCAATTTGATCTAATCCGAATTGTTCAACGACTg                                                                                                               >  2:233827/1‑139 (MQ=255)

                 cGATAAAAACAGGATATGACGAGGAAGCAGTTTGAACATGCAGTGTCTTCATTAAAATTCCCTCGACAGTTTTCTCATATTCTCCACATTTTCTCTAATGCGGTCAATTTGATCTAATCCGAATTGTTCAACGACTGCg                                                                                                             >  8:273677/1‑139 (MQ=255)

                      aaaaCAGGATATGACGAGGAAGCAGTTTGAACATGCAGTGTCTTCATTAAAATTCCCTCGACAGTTTTCTCATATTCTCCACATTTTCTCTAATGCGGTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGc                                                                                                        <  8:429613/139‑1 (MQ=255)

                             gATATGACGAGGAAGCAGTTTGAACATGCAGTGTCTTCATTAAAATTCCCTCGACAGTTTTCTCATATTCTCCACATTTTCTCTAATGCGGTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTcc                                                                                                 >  2:35253/1‑139 (MQ=255)

                                     gaGGAAGCAGTTTGAACATGCAGTGTCTTCATTAAAATTCCCTCGACAGTTTTCTCATATTCTCCACATTTTCTCTAATGCGGTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGa                                                                                          >  5:224281/1‑138 (MQ=255)

                                              gTTTGAACATGCAGTGTCTTCATTAAAATTCCCTCGACAGTTTTCTCATATTCTCCACATTTTCTCTAATGCGGTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTc                                                                                <  8:14756/139‑1 (MQ=255)

                                               tttGATCATGCTGTGTCTTAATTAAAATTCCCTGGACAGTTTTCTCATATTCTCCACATTTTCTCTAATGCGGGCAATTTGATATAATGCTATTTTTTCACCGACTGCGTTCGCAATTTCCCATGCGACAGCAGCTTc                                                                                <  8:119463/138‑1 (MQ=255)

                                                         cAGTGTCTTCATTAAAATTCCCTCGACAGTTTTCTCATATTCTCCACATTTTCTCTAATGCGGTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTccac                                                                     >  2:126382/1‑139 (MQ=255)

                                                         cAGTGTCTTCATTAAAATTCCCTCGACAGTTTTCTCATATTCTCCACATTTTCTCTAATGCGGTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTacac                                                                     >  6:169225/1‑139 (MQ=255)

                                                         cAGTGTCTTCATTAAAATTCCCTCGACAGTTTTCTCATATTCTCCACATTTTCTCTAATGCGGTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTacac                                                                     >  7:403107/1‑139 (MQ=255)

                                                           gtgtCTTCATTAAAATTCCCTCGACAGTTTTCTCATATTATCCACATTTTCTCTAATGCGGTCAATTTGATCTAAACCTAATGTTCCAACGACTGCGTTCGCAATTTCCCACGCGACAGCCGCTTCAGCGACTACACtt                                                                   <  6:33885/139‑1 (MQ=255)

                                                             gtcttCATTAAAATTCCCTCGACAGTTTTCTCATATTCTCCACATTTTCTCTAATGCGGTCAAGTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGc                                                                 >  7:151115/4‑139 (MQ=255)

                                                             gtcttCATTAAAATTCCCTCGACAGTTTTCTCATATTCTCCACATTTTCTCTAATGCGGACAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGc                                                                 >  7:348650/4‑139 (MQ=255)

                                                              tcttCATTAAAATTCCCTCGACAGTTTTCTCATATTCTCCACATTTTCTCTAATGCGGTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGcga                                                                           <  7:386365/126‑3 (MQ=255)

                                                              tcttCATTAAAATTCCCTCGACAGTTTTCTCATATTCTCCACATTTTCTCTAATGCGGTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGcga                                                                           >  8:386365/3‑126 (MQ=255)

                                                              tcttCATTAAAATTCCCTCGACAGTTTTCTCATATTCTCCACATTTTCTCTAATGCGGTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGcc                                                                >  2:59412/3‑139 (MQ=255)

                                                                  aataaaaaTGCCCGCCCCAGTTTACTCATATTCTCCCCATTTTCTCAAATGCGGCAAATTTCATAAAATCCGAATTGTCCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGc                                                              <  6:5800/133‑1 (MQ=255)

                                                                   aTTAACATGCCCTCGACAGTTTTCTCAAATTCTCCACATTTGCTCAAATGCGGGCAATTTGATCAAATCCGAATTGTTCAACGACTGCGTGCGCAAATTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAgg                                                           <  6:437481/139‑1 (MQ=255)

                                                                   aTTAAAATTCCCTCGACAGTTTTCTCATATTCTCCACATTTTCTCTAATGCGGTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAg                                                            >  4:258062/1‑138 (MQ=255)

                                                                    ttAAAATTCCCTCGACAGTTTTCTCATATTCTCCACATTTTCTCTAATGCGGTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGCCTACCCTTGCCGCAgg                                                           >  3:108441/1‑138 (MQ=255)

                                                                    ttAAAATTCCCTCGACAGTTTTCTCATATTCTCCACATTTTCTCTAATGCGGTCAATTTGATCTAATCCGAATTGTTCAACGAATGCGTTAGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAgg                                                           >  8:146871/1‑138 (MQ=255)

                                                                      aaatTTCCCTCGACAGTTTTCTCATATTCTCCACATTTTCTCTAATGCGGTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAggaa                                                         <  2:39212/138‑3 (MQ=255)

                                                                      aaaaTTCCCTCGACAGTTTTCTCATATTCTCCACATTTTCTCTAATGGGGTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAggaa                                                         <  8:151115/138‑3 (MQ=255)

                                                                      aaaaTTCCCTCGACAGTTTTCTCATATTCTCCACATTTTCTCTAATGCGGTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCaa                                                                                                      >  8:416938/1‑93 (MQ=255)

                                                                      aaaaTTCCCTCGACAGTTTTCTCATATTCTCCACATTTTCTCTAATGCGGTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCaa                                                                                                      <  7:416938/93‑1 (MQ=255)

                                                                      aaaaTTCCCTCGACAGTTTTCTCATATTCTCCACATTTTCTCTAATGCGGTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAggaa                                                         <  1:373438/138‑3 (MQ=255)

                                                                      aaaaTTCCCTCGACAGTTTTCTCATATTCTCCACATTTTCTCTAATGCGGTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAggaa                                                         <  8:403107/138‑3 (MQ=255)

                                                                      aaaaTTCCCTCGACAGTTTTCTCATATTCTCCACATTTTCTCTAATGCGGTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAggaa                                                         <  3:224727/138‑3 (MQ=255)

                                                                      aaaaTTCCCTCGACAGTTTTCTCATATTCTCCACATTTTCTCTAATGCGGTCAATTTGATCTAATCCGAATTGTGCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAggaa                                                         <  7:273677/138‑3 (MQ=255)

                                                                             ccTCGACAGTTTTCTCATATTCTCCACATTTTCTCTAATGCGGTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAg                                                 <  2:247110/139‑1 (MQ=255)

                                                                             ccTCGACAGTTTTCTCATATTCTCAACATTTTCTCTAATGCGGTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAg                                                 <  8:337331/139‑1 (MQ=255)

                                                                             ccTCGACAGCTTTCTCATAATCGCCACATATACGCTAATGCCGTCAATATGACCTAAGCCGACTTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAg                                                 <  3:164238/139‑1 (MQ=255)

                                                                               tCGACAGTTTTCTCATATTCTCCACATTTTCTCTAATGCGGTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCt                                               >  5:261707/1‑139 (MQ=255)

                                                                                     gTTTTCTCATATTCTCCATTTTTTCTCTTATGCGGTCAACTTCAACTAATCCCAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGa                                         <  1:340057/139‑1 (MQ=255)

                                                                                     gTTTTCTCATATTCTCCACATTTTCTCTAATGCGGTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGa                                         <  5:169225/139‑1 (MQ=255)

                                                                                     gTTTCCTCATATTCTCCACATTTTCTCTAATGCGGTCAATTTGATCAAATCCGACTTGTTCAACGACTGCGGTCTCAATCTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGa                                         <  7:58997/139‑1 (MQ=255)

                                                                                                ttCTCCACATTTTCTCTAATGCGGTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGCCTACACTTGCCGCAGGAACAGCACAGCTGTCTGAACGTTCAATGc                              >  6:405785/1‑139 (MQ=255)

                                                                                                ttCTCCACATTTTCTCTAATGCGGTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGc                                                              >  6:358060/1‑107 (MQ=255)

                                                                                                ttCTCCACATTTTCTCTAATGCGGTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGc                                                              <  5:358060/107‑1 (MQ=255)

                                                                                                ttCTCCACATTTTCTCTAATGCGGTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAgg                                                           >  7:214238/1‑110 (MQ=255)

                                                                                                ttCTCCACATTTTCTCTAATGCGGTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAgg                                                           <  8:214238/110‑1 (MQ=255)

                                                                                                     cacaTTTTCTCTAATGCGGTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGAACGTTCAATGCTgg                           <  8:424264/137‑1 (MQ=255)

                                                                                                                  aTGCGGTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGAACGTTCAATGCTGGCAGAAAACGGTTCt             <  1:35253/138‑1 (MQ=255)

                                                                                                                       gTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGa                                         <  1:359962/105‑1 (MQ=255)

                                                                                                                       gTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGa                                         >  2:359962/1‑105 (MQ=255)

                                                                                                                       gTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGa                                         >  2:415186/1‑105 (MQ=255)

                                                                                                                       gTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGa                                         >  2:365963/1‑105 (MQ=255)

                                                                                                                       gTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGa                                         <  1:415186/105‑1 (MQ=255)

                                                                                                                       gTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGa                                         <  1:365963/105‑1 (MQ=255)

                                                                                                                       gTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGAACGTTCAATGCTGGCAGAAAACGGTTCTTTCGtt       >  7:301636/1‑139 (MQ=255)

                                                                                                                       gTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGAACGATCAATGCTGGCAGAAAACGGTTCTTTCGtt       >  6:448148/1‑139 (MQ=255)

                                                                                                                          aaTTTGATCTAATCCGACTTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGAACGTTCAATGCTGGCAGAAAACGGTTCTTTCGTTTc     <  8:404363/137‑1 (MQ=255)

                                                                                                                             tttaTCTATTCCCAATTGTTCAACCCCTGGGTGCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGAACGTTCAATGCTGGCAGAAAACGGTTCTTTCGTTTCAat  <  8:395324/135‑1 (MQ=255)

TTTTCTGATGCCTTGTCCGATAAAAACAGGATACGACGAGGAAGCGGTTTGAACATGCAGTGTTTTCATTAAAATTCCCTCGACAGTTTTCTCATATTCTCCACATTTTCTCTAATGCGGTCGATTTGATCTAAGCCGAATTGTTCAACGACTGCGTTCGCAATCTCCCATGCGACAGCCGCTTCAGCTACTACACTTGCCGCAGGCACTGCACAGCTGTCTGAACGTTCAATGCTGGCAGAAAACGGTTCTTTCGTTTCAAT  >  CP006881/2196897‑2197159

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A