Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A14 F56 I20 R2 1958 92.1 3575798 91.1% 3257551 126.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 2,197,229 T→C G303G (GGA→GGG)  U712_11010 ← Chorismate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,197,2290TC100.0% 181.7 / NA 57G303G (GGA→GGG) U712_11010Chorismate synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (33/24);  total (33/24)

TTGCCGCAGGCACTGCACAGCTGTCTGAACGTTCAATGCTGGCAGAAAACGGTTCTTTCGTTTCAATATCTACGCTTTTCAGCGGTTTATACAAAGTCGGAATCGGCTTCATGACTCCGCGGACAACAATCGGCATTCCTGTCGTCATGCCGCCTTCCAGTCCTCCGAGTCTGTTTGTAGCGCGGGTATATCCCTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCTTCCGGCTGCTTCAAAGCCGATGCCGAATTCC  >  CP006881/2197093‑2197364
                                                                                                                                        |                                                                                                                                       
ttGCCGCAGGAACAGCACAGCTGTCTGAACGTTCAATGCTGGCAGAAAACGGTTCTTTCGTTTCAATATCGACACTTTTCAGCGGTTTATACAAAGTCGGAATCGGCTTCATGACTCCGCGGACAACAATCGGCATccc                                                                                                                                       >  6:59107/1‑139 (MQ=255)
     gCAGGAACAGCACAGCTGTCTGAACGTTCAATGCTGGCAGAAAACGGTTCTTTCGTTTCAATATCGACACTTTTCAGCGGTTTATACAAAGTCGGAATCGGCTTCATGACTCCGCGGACAACAATCGGCATCCCTgtcg                                                                                                                                  >  4:256493/1‑139 (MQ=255)
          aacagCACAGCTGTCTGAACGTTCAATGCTGGCAGAAAACGGTTCTTTCGTTTCAATATCGACACTTTTCAGCGGTTTATACAAAGTCGGAATCGGCTTCATGACTCCGCGGACAACAATCGGCATCCCTGTCGTCATg                                                                                                                             <  4:108441/135‑1 (MQ=255)
            cagCACAGCTGTCTGAACGTTCAATGCTGGCAGAAAACGGTTCTTTCGTTTCAATATCGACACTTTTCAGCGGTTTATACAAAGTCGGAATCGGCTTCATGACTCCGCGGACAACAATCGGCATCCCTGTCGTCATgcc                                                                                                                           <  3:320722/137‑1 (MQ=255)
                   gCTGTCTGAACGTTCAATGCTGGCAGAAAACGGTTCTTTCGTTTCAATATCGACACTTTTCAGCGGTTTATACAAAGTCGGAATCGGCTTCATGACTCCGCGGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTcc                                                                                                                    <  2:386925/139‑1 (MQ=255)
                    cTGTCTGAACGTTCAATGCTGGCAGAAAACGGTTCTTTCGTTTCAATATCGACACTTTTCAGCGGTTTATACAAAGTCGGAATCGGCTTCATGACTCCGCGGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTc                                                                                                                     >  6:401933/1‑137 (MQ=255)
                       tCTGAATGTTCAATGCTGGCAGAAAACGGTTCTTTCGTTTCAATATCGACACTTTTCAGCGGTTTATACAAAGTCGGAATCGGCTTCATGACTCCGCGGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGtc                                                                                                                >  8:306941/1‑139 (MQ=255)
                         tGAACGTTCAATGCTGGCAGAAAACGGTTCTTTCGTTTCAATATCGACACTTTTCAGCGGTTTATACAAAGTCGGAATCGGCTTCATGACTCCGCGGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGtccg                                                                                                              >  3:32975/1‑138 (MQ=255)
                         tGAACGTTCAATGCTGGCAGAAAACGGTTCTTTCGTTTCAATATCGACACTTTTCAGCGGTTTATACAAAGTCGGAATCGGCTTCATGACTCCGCGGACAACAATCGGCATCCCTGTCGTCATGCCGCCATACAGtccg                                                                                                              >  1:397004/1‑138 (MQ=255)
                           aaCGTTCAATGCTGGCAGAAAACGGTTCTTTCGTTTCAATATCGACACTTTTCAGCGGTTTATACAAAGTCGGAATCGGCTTCATGACTCCGCGGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGtccgcc                                                                                                            <  3:339548/139‑1 (MQ=255)
                           aaCGTTCAATGCTGGCAGAAAACGGTTCTTTCGTTTCAATATCGACACTTTTCAGCGGTTTATACAAAGTCGGAATCGGCTTCATGACTCCGCGGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGtccg                                                                                                              <  1:59412/137‑2 (MQ=255)
                            aCGTTCAATGCTGGCAGAAAACGGTTCTTTCGTTTCAATATCGACACTTTTCAGCGGTTTATACAAAGTCGGAATCGGCTTCATGACTCCGCGGCCAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGtccgcc                                                                                                            >  4:429308/1‑138 (MQ=255)
                              gTTCAATGCTGGCAGAAAACGGTTCTTTCGTTTCAATATCGACACTTTTCAGCGGTTTATACAAAGTCGGAATCGGCTTCATGACTCCGCGGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGtccgcca                                                                                                           >  4:67751/1‑136 (MQ=255)
                              gTTCAATGCTGGCAGAAAACGGTTCTTTCGTTTCAATATCGACACTTTTCAGCGGTTTATACAAAGTCGGAATCGGCTTCATGACTCCGCGGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGtccgcca                                                                                                           <  3:67751/137‑2 (MQ=255)
                                 cAATGCTGGCAGAAAACGGTTCTTTCGTTTCAATATCGACACTTTTCAGCGGTTTATACAAAGTCGGAATCGGCTTCATGACTCCGCGGACAACAATCGGCATCCCTGTCGTCATg                                                                                                                             >  8:332493/1‑116 (MQ=255)
                                 cAATGCTGGCAGAAAACGGTTCTTTCGTTTCAATATCGACACTTTTCAGCGGTTTATACAAAGTCGGAATCGGCTTCATGACTCCGCGGACAACAATCGGCATCCCTGTCGTCATg                                                                                                                             <  7:332493/116‑1 (MQ=255)
                                 cAATGCTGGCAGAAAACGGTTCTTTCGTTTCAATATCGACACTTTTCAGCGGTTTATACAAAGTCGGAATCGGCTTCATGACTCCGCGGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCt                                                                                                      >  7:445879/1‑139 (MQ=255)
                                    tGCTGGCAGAAAACGGTTCTTTCGTTTCAATATCGACACTTTTCAGCGGTTTATACAAAGTCGGAATCGGCTTCATGACTCCGCGGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGtt                                                                                                   <  4:213793/139‑1 (MQ=255)
                                      cTGGCAGAAAACGGTTCTTTCGTTTCAATATCGACACTTTTCAGCGGTTTATACAAAGTCGGAATCGGCTTCATGACTCCGCGGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGttag                                                                                                 <  8:321943/139‑3 (MQ=255)
                                       ttgCAGAAAACGGTTCTTTCGTTTCAATATCGACACTTTTCAGCGGTTTATACAAAGTAGGAATCGTCTTCATGACTCCGCGGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGt                                                                                                <  5:448148/137‑1 (MQ=255)
                                          cAGAAAACGGTTCTTTCGTTTCAATATCCACACTTTTCAGCGGTTTATACAAAGTCGTAAGCGGCTTCATGCCTCCGCGGACAACAATCGGCATCCCTGTCGTCATGCCGCATACCAGTCCGACAATTCTGttattag                                                                                              >  6:99702/1‑133 (MQ=255)
                                           aGAAAACGGTTCTTTCGTTTCAATATCGACACTTTTCAGCGGTTTATACAAAGTCGGAATCGGCTTCATGACTCCGCGGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAgca                                                                                            <  3:256493/139‑2 (MQ=255)
                                           aGAAAACGGTTCTTTAGTTTCAATATCGACACTTTTCAGCGGTTTATACAAAGTCGGAATCGGCTTCATGACTCCGCGGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAgca                                                                                            <  8:445879/139‑2 (MQ=255)
                                            gAAAACGGTTCTTTCGTTTCAATATCGACACTTTTCAGCGGTTTATACAAAGTCGGAATCGGCTTCATGACTCCGCGGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAgcac                                                                                           <  2:49339/139‑3 (MQ=255)
                                             aaaaCGGTTCTTTCGTTTCAATATCGACACTTTTCAGCGGTTTATACAAAGTCGGAATCGGATTCATGACTCCGCGGACAACAATCGGCATCCCTGTCGTCATGCCGACTTCCAGTCCGCCAAGTCTGTTAGTAgcaag                                                                                          >  8:233716/1‑136 (MQ=255)
                                                 cGGTTCTTTCGTTTCAATCTCGACACTTTTCAGCGGTTTATACAAAGTCGGAATCGGCTTCATGACTCCGCGGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGtg                                                                                      <  6:261707/139‑2 (MQ=255)
                                                       tttCGTTTCAATATCGACACTTTTCAGCGGTTTATACAAAGTCGGAATCGGCTTCATGACTCCGCGGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGGCTGTTAGTAGCACGGGTGTATcct                                                                                >  2:375946/1‑138 (MQ=255)
                                                          cGTTTCAATATCGACACTTTTCAGCGGTTTATACAAAGTCGGAATCGGCTTCATGACTCAGCGTACAACAATCGGCATCCCTGTCGTAATTCCGCCTTCCAGTCAGCCAAGTCTGTTATTAGCACGGGTGTTTCCtttt                                                                             >  6:315636/1‑139 (MQ=255)
                                                            tttCAATATCGACACTTTTCAGCGGTTTATACAAAGTCGGAATCGGCTTCATGACTCCGCGGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTc                                                                           >  7:337300/1‑139 (MQ=255)
                                                               cAATATCGACACTTTTCAGCGGTTTATACAAAGTCGGAATCGGCTTCATGACTCCGCGGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCt                                                                         <  7:146871/138‑1 (MQ=255)
                                                                      gacacTTTTAAGCGGTTTATACAAAGTCGGAATCGGCTTCATGACTCCGCGGACAACAATAGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCaa                                                                 <  6:288502/135‑1 (MQ=255)
                                                                             ttCAGCGGTTTATACAAAGTCGGAATCGGCTTCATGACTCCGCGGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGAtct                                                          >  3:14687/1‑139 (MQ=255)
                                                                             ttCAGCGGTTTATACAAAGTCGGAATCGGCTTCATGACTCCGCGGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGAtct                                                          >  6:91346/1‑139 (MQ=255)
                                                                             ttCAGCGGTTTATACAAAGTCGGAATCGGCTTCATGACTCCGCGGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGAtc                                                           <  1:126382/138‑1 (MQ=255)
                                                                                  ctgTTTATAGAAAGTCGGAATCGTCTTCATGACTCCGCGGACAACAATCGTCATCCCTGTCGTCATGCCGCCTTCGAGTCCGACAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCa                                                     <  6:134870/137‑1 (MQ=255)
                                                                                     tttATACAAAGTCGGAATCGGCTTCATGACTCCGCGGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTATTAGCACGGGTGTATCATTTTTCCTCTTCCCAAATGATATCGTCATg                                                   >  5:134870/1‑138 (MQ=255)
                                                                                     tttATACAAAGTCGGAATCGGCTTCATGACTCCGCGGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATgg                                                  >  6:412915/1‑139 (MQ=255)
                                                                                       tataCAAAGTCGGAATCGGCTTCATGACTCCGCGGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTTGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGAc                                                >  4:423553/1‑139 (MQ=255)
                                                                                       tataCAAAGTCGGAATCGGCTTCATGACTCCGCGGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGa                                                 <  8:348650/138‑1 (MQ=255)
                                                                                             aaGTCGGAATCGGCTTCATGACTCCGCGGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCt                                          <  5:91346/139‑1 (MQ=255)
                                                                                              aGTCGGAATCGGCTTCATGACTCCGCGGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGtccgcc                                                                                                            <  1:100485/72‑1 (MQ=255)
                                                                                              aGTCGGAATCGGCTTCATGACTCCGCGGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGtccgcc                                                                                                            >  2:100485/1‑72 (MQ=255)
                                                                                              aGTCGGAATCGGCTTCATGACTCCGCGGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGAtc                                                           >  7:104759/1‑121 (MQ=255)
                                                                                              aGTCGGAATCGGCTTCATGACTCCGCGGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGAtc                                                           <  8:104759/121‑1 (MQ=255)
                                                                                              aGTCGGAATCGGCTTCATGACTCCGCGGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGAtc                                                           >  5:72761/1‑121 (MQ=255)
                                                                                              aGTCGGAATCGGCTTCATGACTCCGCGGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCtt                                         >  2:7603/1‑139 (MQ=255)
                                                                                              aGTCGGAATCGGCTTCATGACTCCGCGGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCtt                                         >  1:401808/1‑139 (MQ=255)
                                                                                              aGTCGGAATCGGCTTCATGACTCCGCGGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCtt                                         >  1:17903/1‑139 (MQ=255)
                                                                                              aGTCGGAATCGGCTTCATGACTCCGCGGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCt                                          >  4:126194/1‑138 (MQ=255)
                                                                                              aGTCGGAATCGGCTTCATGACTCCGCGGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGGGTATCCTTTTTCCTCGTCCCAAATGAtc                                                           <  6:72761/121‑1 (MQ=255)
                                                                                                        ggCTTCATGACTCCGCGGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATttcgcc                               >  8:238416/1‑136 (MQ=255)
                                                                                                                     cgcgGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCaaa                  >  5:186762/1‑139 (MQ=255)
                                                                                                                     cgcgGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCaaa                  >  1:216402/1‑139 (MQ=255)
                                                                                                                     cgcgGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCaa                   >  7:351700/1‑138 (MQ=255)
                                                                                                                       cgGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCaaatc                >  7:302402/1‑137 (MQ=255)
                                                                                                                       cgGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCACTCCGCCAACTCTGTTAGTATCACCGGTGTATACTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTACATTTCGCCCTGCTGCCTCaaatc                >  4:151282/1‑137 (MQ=255)
                                                                                                                                 tCGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGACCTGCTGCCTCAAATCCGATGcgcaa      >  5:385499/1‑135 (MQ=255)
                                                                                                                                     cATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTcc  <  5:405785/139‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                       
TTGCCGCAGGCACTGCACAGCTGTCTGAACGTTCAATGCTGGCAGAAAACGGTTCTTTCGTTTCAATATCTACGCTTTTCAGCGGTTTATACAAAGTCGGAATCGGCTTCATGACTCCGCGGACAACAATCGGCATTCCTGTCGTCATGCCGCCTTCCAGTCCTCCGAGTCTGTTTGTAGCGCGGGTATATCCCTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCTTCCGGCTGCTTCAAAGCCGATGCCGAATTCC  >  CP006881/2197093‑2197364

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A