Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A14 F56 I20 R2 1958 92.1 3575798 91.1% 3257551 126.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 2,197,274 G→A R288R (CGC→CGT)  U712_11010 ← Chorismate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,197,2740GA100.0% 164.6 / NA 58R288R (CGC→CGT) U712_11010Chorismate synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (37/21);  total (37/21)

CGGTTCTTTCGTTTCAATATCTACGCTTTTCAGCGGTTTATACAAAGTCGGAATCGGCTTCATGACTCCGCGGACAACAATCGGCATTCCTGTCGTCATGCCGCCTTCCAGTCCTCCGAGTCTGTTTGTAGCGCGGGTATATCCCTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCTTCCGGCTGCTTCAAAGCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCAGCAGCAAGCTTG  >  CP006881/2197142‑2197409
                                                                                                                                    |                                                                                                                                       
cGGTTCTTTCGTTTCAATCTCGACACTTTTCAGCGGTTTATACAAAGTCGGAATCGGCTTCATGACTCCGCGGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGtg                                                                                                                                   <  6:261707/139‑2 (MQ=255)
      tttCGTTTCAATATCGACACTTTTCAGCGGTTTATACAAAGTCGGAATCGGCTTCATGACTCCGCGGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGGCTGTTAGTAGCACGGGTGTATcct                                                                                                                             >  2:375946/1‑138 (MQ=255)
         cGTTTCAATATCGACACTTTTCAGCGGTTTATACAAAGTCGGAATCGGCTTCATGACTCAGCGTACAACAATCGGCATCCCTGTCGTAATTCCGCCTTCCAGTCAGCCAAGTCTGTTATTAGCACGGGTGTTTCCtttt                                                                                                                          >  6:315636/1‑139 (MQ=255)
           tttCAATATCGACACTTTTCAGCGGTTTATACAAAGTCGGAATCGGCTTCATGACTCCGCGGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTc                                                                                                                        >  7:337300/1‑139 (MQ=255)
              cAATATCGACACTTTTCAGCGGTTTATACAAAGTCGGAATCGGCTTCATGACTCCGCGGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCt                                                                                                                      <  7:146871/138‑1 (MQ=255)
                     gacacTTTTAAGCGGTTTATACAAAGTCGGAATCGGCTTCATGACTCCGCGGACAACAATAGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCaa                                                                                                              <  6:288502/135‑1 (MQ=255)
                            ttCAGCGGTTTATACAAAGTCGGAATCGGCTTCATGACTCCGCGGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGAtct                                                                                                       >  6:91346/1‑139 (MQ=255)
                            ttCAGCGGTTTATACAAAGTCGGAATCGGCTTCATGACTCCGCGGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGAtct                                                                                                       >  3:14687/1‑139 (MQ=255)
                            ttCAGCGGTTTATACAAAGTCGGAATCGGCTTCATGACTCCGCGGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGAtc                                                                                                        <  1:126382/138‑1 (MQ=255)
                                 ctgTTTATAGAAAGTCGGAATCGTCTTCATGACTCCGCGGACAACAATCGTCATCCCTGTCGTCATGCCGCCTTCGAGTCCGACAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCa                                                                                                  <  6:134870/137‑1 (MQ=255)
                                    tttATACAAAGTCGGAATCGGCTTCATGACTCCGCGGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTATTAGCACGGGTGTATCATTTTTCCTCTTCCCAAATGATATCGTCATg                                                                                                >  5:134870/1‑138 (MQ=255)
                                    tttATACAAAGTCGGAATCGGCTTCATGACTCCGCGGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATgg                                                                                               >  6:412915/1‑139 (MQ=255)
                                      tataCAAAGTCGGAATCGGCTTCATGACTCCGCGGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTTGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGAc                                                                                             >  4:423553/1‑139 (MQ=255)
                                      tataCAAAGTCGGAATCGGCTTCATGACTCCGCGGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGa                                                                                              <  8:348650/138‑1 (MQ=255)
                                            aaGTCGGAATCGGCTTCATGACTCCGCGGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCt                                                                                       <  5:91346/139‑1 (MQ=255)
                                             aGTCGGAATCGGCTTCATGACTCCGCGGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGAtc                                                                                                        >  7:104759/1‑121 (MQ=255)
                                             aGTCGGAATCGGCTTCATGACTCCGCGGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGAtc                                                                                                        <  8:104759/121‑1 (MQ=255)
                                             aGTCGGAATCGGCTTCATGACTCCGCGGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGAtc                                                                                                        >  5:72761/1‑121 (MQ=255)
                                             aGTCGGAATCGGCTTCATGACTCCGCGGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCtt                                                                                      >  1:17903/1‑139 (MQ=255)
                                             aGTCGGAATCGGCTTCATGACTCCGCGGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCtt                                                                                      >  1:401808/1‑139 (MQ=255)
                                             aGTCGGAATCGGCTTCATGACTCCGCGGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCtt                                                                                      >  2:7603/1‑139 (MQ=255)
                                             aGTCGGAATCGGCTTCATGACTCCGCGGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCt                                                                                       >  4:126194/1‑138 (MQ=255)
                                             aGTCGGAATCGGCTTCATGACTCCGCGGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGGGTATCCTTTTTCCTCGTCCCAAATGAtc                                                                                                        <  6:72761/121‑1 (MQ=255)
                                                       ggCTTCATGACTCCGCGGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATttcgcc                                                                            >  8:238416/1‑136 (MQ=255)
                                                                    cgcgGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCaaa                                                               >  1:216402/1‑139 (MQ=255)
                                                                    cgcgGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCaaa                                                               >  5:186762/1‑139 (MQ=255)
                                                                    cgcgGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCaa                                                                >  7:351700/1‑138 (MQ=255)
                                                                      cgGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCaaatc                                                             >  7:302402/1‑137 (MQ=255)
                                                                      cgGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCACTCCGCCAACTCTGTTAGTATCACCGGTGTATACTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTACATTTCGCCCTGCTGCCTCaaatc                                                             >  4:151282/1‑137 (MQ=255)
                                                                                tCGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGACCTGCTGCCTCAAATCCGATGcgcaa                                                   >  5:385499/1‑135 (MQ=255)
                                                                                    cATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTcc                                               <  5:405785/139‑1 (MQ=255)
                                                                                     atcccTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCca                                              >  8:287665/4‑139 (MQ=255)
                                                                                     atcccTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCca                                              <  7:238416/136‑1 (MQ=255)
                                                                                     atcccTGTCGTCATGACGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCca                                              <  1:375946/136‑1 (MQ=255)
                                                                                      tcccTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCcac                                             <  5:59107/137‑1 (MQ=255)
                                                                                      tcccTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCcac                                             <  5:412915/137‑1 (MQ=255)
                                                                                      tcccTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTATCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCcac                                             <  5:99702/137‑1 (MQ=255)
                                                                                       cccTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCa                                                                                                  >  7:165719/2‑85 (MQ=255)
                                                                                       cccTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCa                                                                                                  <  8:165719/84‑1 (MQ=255)
                                                                                       cccTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCcaca                                            >  4:399272/2‑139 (MQ=255)
                                                                                       cccTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCcaca                                            >  5:16561/2‑139 (MQ=255)
                                                                                        ccTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCGCAAATCCGATGCCGAATTCCacac                                           >  2:59021/1‑139 (MQ=255)
                                                                                                 aTGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATg                                  >  1:364205/1‑139 (MQ=255)
                                                                                                 aTGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATg                                  >  8:21503/1‑139 (MQ=255)
                                                                                                  tGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGc                                 >  8:103443/1‑139 (MQ=255)
                                                                                                   gccgccTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCg                                >  7:276400/1‑139 (MQ=255)
                                                                                                    ccgccTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGt                               >  8:72275/1‑139 (MQ=255)
                                                                                                      gccTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTa                             <  2:397004/139‑1 (MQ=255)
                                                                                                         ttCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAAtt                          >  4:343039/1‑139 (MQ=255)
                                                                                                            cAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGa                        <  6:16561/138‑1 (MQ=255)
                                                                                                                 cgccaaGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCca                                              >  3:236886/6‑111 (MQ=255)
                                                                                                                 cgccaaGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCca                                              <  4:236886/106‑1 (MQ=255)
                                                                                                                    caaGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAAcagc               >  2:301238/3‑139 (MQ=255)
                                                                                                                            ttagTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACagcggcagc         >  6:445186/4‑137 (MQ=255)
                                                                                                                            ttagTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTACCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACagcggcagc         >  3:183935/4‑137 (MQ=255)
                                                                                                                               ggaGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCt    <  7:306941/137‑1 (MQ=255)
                                                                                                                               gTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCt    <  2:401808/139‑1 (MQ=255)
                                                                                                                                 aGCACGGGTGTATCCTTTTTCATCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCAGATTCCTAAATCCACACCTTTAAATGCGTTAATTTACAGAACAGCGCCAGCAAGCttc  >  8:267165/1‑138 (MQ=255)
                                                                                                                                    |                                                                                                                                       
CGGTTCTTTCGTTTCAATATCTACGCTTTTCAGCGGTTTATACAAAGTCGGAATCGGCTTCATGACTCCGCGGACAACAATCGGCATTCCTGTCGTCATGCCGCCTTCCAGTCCTCCGAGTCTGTTTGTAGCGCGGGTATATCCCTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCTTCCGGCTGCTTCAAAGCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCAGCAGCAAGCTTG  >  CP006881/2197142‑2197409

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A