Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A14 F56 I20 R2
|
1958 |
92.1 |
3575798 |
91.1% |
3257551 |
126.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
CP006881 |
2,197,505 |
G→T |
A211A (GCC→GCA) |
U712_11010 ← |
Chorismate synthase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | CP006881 | 2,197,505 | 0 | G | T | 100.0%
| 174.1
/ NA
| 56 | A211A (GCC→GCA) | U712_11010 | Chorismate synthase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (24/32); total (24/32) |
TAAATGCGTTAATTGACAGAACAGCAGCAGCAAGCTTGCTGTCCAGTTTGCGGTCATAATGGACGTAGCTGCCAACGCCTACTGGCATTCCCTCGACAATGACTTCTACAATTCCGCCGATGGAATCTCCGTTGGCCTTCGCTTCATCAATGGCAGCCATCATTTTTTTGCCCGCTTCTTCATCGTAGCATCTGACCGGAGACTCCTCCGTTACACGCTGCAGGTCATCAATTGACGTATATTCTGTTTTTTCAGCTTTAACTGCGCCA > CP006881/2197372‑2197640
|
tAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAATGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGcc < 3:41984/137‑1 (MQ=255)
tAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAATGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGcc < 3:74556/137‑1 (MQ=255)
gTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAATGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTtca > 8:21415/1‑139 (MQ=255)
gTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAATGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTtca > 5:164864/1‑139 (MQ=255)
gTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAATGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTtca > 6:326869/1‑139 (MQ=255)
gTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAATGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTtca > 7:222200/1‑139 (MQ=255)
aGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAATGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAg > 1:423868/1‑139 (MQ=255)
aaCAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAATGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGc > 5:255520/1‑138 (MQ=255)
cggcagcaAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAATGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCcatcat > 5:164418/3‑139 (MQ=255)
cggcagcaAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAATGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCcatcat > 5:172034/3‑139 (MQ=255)
aGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAATGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGc > 4:169575/1‑139 (MQ=255)
aGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAATGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGc > 3:84073/1‑139 (MQ=255)
gCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAATGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGcc < 4:84073/139‑1 (MQ=255)
gCTTGCTATCCAGCTTGCGGTCATAATGCACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAATGACTTCTACTAATCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGcc < 6:385499/139‑1 (MQ=255)
gCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAATGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGc < 7:233716/138‑1 (MQ=255)
ctatCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAATGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGcct < 6:255520/136‑3 (MQ=255)
cttGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAATGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCg < 5:170836/138‑1 (MQ=255)
tCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAATGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATc < 7:149283/139‑1 (MQ=255)
gACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAATGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGga < 5:395109/139‑1 (MQ=255)
catAGCTGCCCACGCCTACCGGCATTCCCTCGACAATGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGcct > 8:197579/3‑112 (MQ=255)
catAGCTGCCCACGCCTACCGGCATTCCCTCGACAATGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGcct < 7:197579/112‑3 (MQ=255)
catAGCTGCCCACGCCTACCGGCATTCCCTCGACAATGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGgaga < 6:164418/137‑1 (MQ=255)
catAGCTGCCCACGCCTACCGGCATTCCCTCGACAATGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGgaga < 1:7603/137‑1 (MQ=255)
catAGCTGCCCACGCCTACCGGCATTCCCTCGACAATGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGAAAGgaga < 8:222200/137‑1 (MQ=255)
aGCTGCCCACGCCTACCGGCATTCCCTCGACAATGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGAct > 6:251040/1‑138 (MQ=255)
gCTGCCCACGCCTACCGGCATTCCCTCGACAATGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGActc < 3:423553/138‑1 (MQ=255)
gCTGCCCACGCCTACCGGCATTCCCTCGACAATGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGActc < 5:326869/138‑1 (MQ=255)
gCTGCCCACGCCTACCGGCATTCCCTCGACAATGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGActc < 7:287665/138‑1 (MQ=255)
cccaCGCCTACCGGCATTCCCTCGACAATGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGACTCTTCCg > 1:357099/4‑139 (MQ=255)
gCCTACCGGCATTCCCTCGACAATGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGTCAGCCAACATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGACTCTTCCGTTaca > 2:194088/1‑139 (MQ=255)
gCCTACCGGCATTCCCTCGACAATGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGACTCTTCCGTTaca < 3:438631/139‑1 (MQ=255)
gCCTACCGGCATTCCCTCGACAATGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGACTCTTCCGTTaca < 6:164864/139‑1 (MQ=255)
gCCTACCGGCATTCCCTCGACAATGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGCCTCGTCATCGGAGCATCTGACAGGCGACTGTTCCGGTacg > 1:184932/1‑138 (MQ=255)
ccggCATTCCCTCGACAATGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGACTCTTCCGTTACACGCTg < 8:147048/137‑1 (MQ=255)
ccggCATTCCCTCGACAATGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGACTCTTCCGTTACACGCTg > 2:79348/3‑139 (MQ=255)
gACAATGTCTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCaa < 3:378828/137‑1 (MQ=255)
gACAATGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCaa < 7:21415/137‑1 (MQ=255)
gACAATGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCaa < 2:17903/137‑1 (MQ=255)
gACAATGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCAAtt < 1:79348/139‑1 (MQ=255)
aaTGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCAATTGAc < 4:234252/139‑1 (MQ=255)
aaTGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCAATTGAc < 2:200164/139‑1 (MQ=255)
tGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCAAATGACg > 4:142479/1‑138 (MQ=255)
ttCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGCCTCTTCCTCGTAGCATCTGACAGGAGACTCTTCCGTTACCCGCTGCAAGTCTTCAATTGACGTATACTCTGtttt > 3:81987/1‑139 (MQ=255)
ttCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGtttt > 7:99477/1‑139 (MQ=255)
ttCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGtttt > 8:56492/1‑139 (MQ=255)
ccgccgATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTTcc > 2:426245/1‑59 (MQ=255)
ccgccgATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGcc < 1:426245/59‑1 (MQ=255)
ccgccgATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGttttt < 2:345883/138‑1 (MQ=255)
ccgccgATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGttttt < 2:357099/138‑1 (MQ=255)
ccgccgATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGttttt > 3:54023/1‑138 (MQ=255)
ccgccgATGGAATCTACGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGttttt < 2:383465/138‑1 (MQ=255)
cgccgATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTc < 8:99477/139‑1 (MQ=255)
ccgATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAg > 5:357860/1‑139 (MQ=255)
cGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGActc > 2:257032/1‑76 (MQ=255)
cGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGActc < 1:257032/76‑1 (MQ=255)
gTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCa < 4:54023/139‑1 (MQ=255)
|
TAAATGCGTTAATTGACAGAACAGCAGCAGCAAGCTTGCTGTCCAGTTTGCGGTCATAATGGACGTAGCTGCCAACGCCTACTGGCATTCCCTCGACAATGACTTCTACAATTCCGCCGATGGAATCTCCGTTGGCCTTCGCTTCATCAATGGCAGCCATCATTTTTTTGCCCGCTTCTTCATCGTAGCATCTGACCGGAGACTCCTCCGTTACACGCTGCAGGTCATCAATTGACGTATATTCTGTTTTTTCAGCTTTAACTGCGCCA > CP006881/2197372‑2197640
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
