Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A14 F56 I20 R2 1958 92.1 3575798 91.1% 3257551 126.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 2,197,577 C→T E187E (GAG→GAA)  U712_11010 ← Chorismate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,197,5770CT100.0% 150.7 / NA 49E187E (GAG→GAA) U712_11010Chorismate synthase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (26/23);  total (26/23)

CCAACGCCTACTGGCATTCCCTCGACAATGACTTCTACAATTCCGCCGATGGAATCTCCGTTGGCCTTCGCTTCATCAATGGCAGCCATCATTTTTTTGCCCGCTTCTTCATCGTAGCATCTGACCGGAGACTCCTCCGTTACACGCTGCAGGTCATCAATTGACGTATATTCTGTTTTTTCAGCTTTAACTGCGCCAATTTGCAATACGTGACCTGCCACCTTAATGCCGAGCTCAGAAAGAATCTTTTTAGCGACAGCTCCTGCCGC  >  CP006881/2197443‑2197711
                                                                                                                                      |                                                                                                                                      
cccaCGCCTACCGGCATTCCCTCGACAATGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGACTCTTCCg                                                                                                                                    >  1:357099/4‑139 (MQ=255)
     gCCTACCGGCATTCCCTCGACAATGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGTCAGCCAACATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGACTCTTCCGTTaca                                                                                                                               >  2:194088/1‑139 (MQ=255)
     gCCTACCGGCATTCCCTCGACAATGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGACTCTTCCGTTaca                                                                                                                               <  6:164864/139‑1 (MQ=255)
     gCCTACCGGCATTCCCTCGACAATGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGACTCTTCCGTTaca                                                                                                                               <  3:438631/139‑1 (MQ=255)
     gCCTACCGGCATTCCCTCGACAATGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGCCTCGTCATCGGAGCATCTGACAGGCGACTGTTCCGGTacg                                                                                                                               >  1:184932/1‑138 (MQ=255)
          ccggCATTCCCTCGACAATGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGACTCTTCCGTTACACGCTg                                                                                                                          <  8:147048/137‑1 (MQ=255)
          ccggCATTCCCTCGACAATGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGACTCTTCCGTTACACGCTg                                                                                                                          >  2:79348/3‑139 (MQ=255)
                       gACAATGTCTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCaa                                                                                                               <  3:378828/137‑1 (MQ=255)
                       gACAATGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCaa                                                                                                               <  2:17903/137‑1 (MQ=255)
                       gACAATGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCaa                                                                                                               <  7:21415/137‑1 (MQ=255)
                       gACAATGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCAAtt                                                                                                             <  1:79348/139‑1 (MQ=255)
                          aaTGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCAATTGAc                                                                                                          <  2:200164/139‑1 (MQ=255)
                          aaTGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCAATTGAc                                                                                                          <  4:234252/139‑1 (MQ=255)
                            tGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCAAATGACg                                                                                                         >  4:142479/1‑138 (MQ=255)
                                        ttCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGCCTCTTCCTCGTAGCATCTGACAGGAGACTCTTCCGTTACCCGCTGCAAGTCTTCAATTGACGTATACTCTGtttt                                                                                            >  3:81987/1‑139 (MQ=255)
                                        ttCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGtttt                                                                                            >  8:56492/1‑139 (MQ=255)
                                        ttCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGtttt                                                                                            >  7:99477/1‑139 (MQ=255)
                                          ccgccgATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGttttt                                                                                           <  2:345883/138‑1 (MQ=255)
                                          ccgccgATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGttttt                                                                                           >  3:54023/1‑138 (MQ=255)
                                          ccgccgATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGttttt                                                                                           <  2:357099/138‑1 (MQ=255)
                                          ccgccgATGGAATCTACGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGttttt                                                                                           <  2:383465/138‑1 (MQ=255)
                                           cgccgATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTc                                                                                         <  8:99477/139‑1 (MQ=255)
                                             ccgATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAg                                                                                       >  5:357860/1‑139 (MQ=255)
                                                           gTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCa                                                                         <  4:54023/139‑1 (MQ=255)
                                                              tgCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCAATTGACGTATACTc                                                                                                  <  8:229398/110‑1 (MQ=255)
                                                              tgCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCAATTGACGTATACTc                                                                                                  >  7:229398/2‑111 (MQ=255)
                                                                 cTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGc                                                                   <  3:169575/139‑1 (MQ=255)
                                                                          atcaATGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTc                                                                                         <  5:174221/108‑1 (MQ=255)
                                                                          atcaATGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTc                                                                                         >  6:174221/1‑108 (MQ=255)
                                                                          atcaATGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTgg                                                          >  7:3946/1‑138 (MQ=255)
                                                                               tttcatccATCTTTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATATGCAACACGTGGCCCGc                                                     <  1:424370/133‑1 (MQ=255)
                                                                               tGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGc                                                     <  5:251040/139‑1 (MQ=255)
                                                                               tGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGc                                                     <  6:357860/139‑1 (MQ=255)
                                                                                    gCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCt                                                <  1:194088/139‑1 (MQ=255)
                                                                                    gCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGACTCTTCCGGTAAACGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCACGCCACCt                                                >  6:212272/1‑139 (MQ=255)
                                                                                                        ctcttcATCGAAGCTTCTGACAGGTGACTCTTCCGTTACACGCTGCATGGCTTCAATTGCCTTATACTCTGTTTTTTCAGCTTTAACAGCACCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCagaaaga                            <  4:202173/138‑1 (MQ=255)
                                                                                                                  tAGCATCTGACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAg                  >  4:364041/1‑139 (MQ=255)
                                                                                                                   aGCATCTGACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGc                 >  7:429960/1‑139 (MQ=255)
                                                                                                                   aGCATATGACAGGATACTCTTCATTAACAAGCAGCAAGTCTTCAAATGACGTATACTCTCTATTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCACGCCACCTTAATGCCAAGCTCAGAAAGAATCtttt                     >  6:90409/1‑135 (MQ=255)
                                                                                                                       tCTGACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGcta               >  3:56044/1‑135 (MQ=255)
                                                                                                                       tCTGACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGcta               >  5:279050/1‑135 (MQ=255)
                                                                                                                          gACAGGAGACTCTTCCGTTACCCGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCAAATTTGCAACACGTGGCCAGCCACCTTAATGACAAGCTAAGAAAGAATCTTTTTAGCTACatcc          >  8:22065/1‑136 (MQ=255)
                                                                                                                          gACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGcc          >  5:439260/1‑138 (MQ=255)
                                                                                                                          gACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGcc          >  8:15602/1‑138 (MQ=255)
                                                                                                                          gACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGcc          >  8:74225/1‑138 (MQ=255)
                                                                                                                            caggAGACTCTTCCGTTACACGCTGCAAGTCTTCAAATGACGTATACTCTGTATATTCAGCAATAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGcccc        <  3:142479/137‑1 (MQ=255)
                                                                                                                               gagaCTCTTCCGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCGTAATGCCAAGCTCAGAAAGAATCTTGTTAGCTACAGCCCCTgc     >  8:169235/1‑139 (MQ=255)
                                                                                                                                  aCTCTTCCGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTgccgc  >  7:180844/1‑139 (MQ=255)
                                                                                                                                  aCTCTTCCGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGAAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTgccgc  >  4:134128/1‑139 (MQ=255)
                                                                                                                                      |                                                                                                                                      
CCAACGCCTACTGGCATTCCCTCGACAATGACTTCTACAATTCCGCCGATGGAATCTCCGTTGGCCTTCGCTTCATCAATGGCAGCCATCATTTTTTTGCCCGCTTCTTCATCGTAGCATCTGACCGGAGACTCCTCCGTTACACGCTGCAGGTCATCAATTGACGTATATTCTGTTTTTTCAGCTTTAACTGCGCCAATTTGCAATACGTGACCTGCCACCTTAATGCCGAGCTCAGAAAGAATCTTTTTAGCGACAGCTCCTGCCGC  >  CP006881/2197443‑2197711

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A