Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A14 F56 I20 R2	1958	92.1	3575798	91.1%	3257551	126.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,197,598	A→T	D180E (GAT→GAA)	U712_11010 ←	Chorismate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,197,598	0	A	T	100.0%	168.7 / NA	54	D180E (GAT→GAA)	U712_11010	Chorismate synthase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (31/23); total (31/23)

GACAATGACTTCTACAATTCCGCCGATGGAATCTCCGTTGGCCTTCGCTTCATCAATGGCAGCCATCATTTTTTTGCCCGCTTCTTCATCGTAGCATCTGACCGGAGACTCCTCCGTTACACGCTGCAGGTCATCAATTGACGTATATTCTGTTTTTTCAGCTTTAACTGCGCCAATTTGCAATACGTGACCTGCCACCTTAATGCCGAGCTCAGAAAGAATCTTTTTAGCGACAGCTCCTGCCGCCACTCTGACAGTTGTTTCC  >  CP006881/2197466‑2197730

gACAATGTCTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCaa                                                                                                                                  <  3:378828/137‑1 (MQ=255)

gACAATGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCaa                                                                                                                                  <  2:17903/137‑1 (MQ=255)

gACAATGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCaa                                                                                                                                  <  7:21415/137‑1 (MQ=255)

gACAATGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCAAtt                                                                                                                                <  1:79348/139‑1 (MQ=255)

   aaTGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCAATTGAc                                                                                                                             <  4:234252/139‑1 (MQ=255)

   aaTGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCAATTGAc                                                                                                                             <  2:200164/139‑1 (MQ=255)

     tGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCAAATGACg                                                                                                                            >  4:142479/1‑138 (MQ=255)

                 ttCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGCCTCTTCCTCGTAGCATCTGACAGGAGACTCTTCCGTTACCCGCTGCAAGTCTTCAATTGACGTATACTCTGtttt                                                                                                               >  3:81987/1‑139 (MQ=255)

                 ttCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGtttt                                                                                                               >  8:56492/1‑139 (MQ=255)

                 ttCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGtttt                                                                                                               >  7:99477/1‑139 (MQ=255)

                   ccgccgATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGttttt                                                                                                              <  2:345883/138‑1 (MQ=255)

                   ccgccgATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGttttt                                                                                                              <  2:357099/138‑1 (MQ=255)

                   ccgccgATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGttttt                                                                                                              >  3:54023/1‑138 (MQ=255)

                   ccgccgATGGAATCTACGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGttttt                                                                                                              <  2:383465/138‑1 (MQ=255)

                    cgccgATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTc                                                                                                            <  8:99477/139‑1 (MQ=255)

                      ccgATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAg                                                                                                          >  5:357860/1‑139 (MQ=255)

                                    gTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCa                                                                                            <  4:54023/139‑1 (MQ=255)

                                       tgCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCAATTGACGTATACTc                                                                                                                     >  7:229398/2‑111 (MQ=255)

                                       tgCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCAATTGACGTATACTc                                                                                                                     <  8:229398/110‑1 (MQ=255)

                                          cTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGc                                                                                      <  3:169575/139‑1 (MQ=255)

                                                   atcaATGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTc                                                                                                            >  6:174221/1‑108 (MQ=255)

                                                   atcaATGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTc                                                                                                            <  5:174221/108‑1 (MQ=255)

                                                   atcaATGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTgg                                                                             >  7:3946/1‑138 (MQ=255)

                                                        tttcatccATCTTTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATATGCAACACGTGGCCCGc                                                                        <  1:424370/133‑1 (MQ=255)

                                                        tGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGc                                                                        <  6:357860/139‑1 (MQ=255)

                                                        tGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGc                                                                        <  5:251040/139‑1 (MQ=255)

                                                             gCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCt                                                                   <  1:194088/139‑1 (MQ=255)

                                                             gCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGACTCTTCCGGTAAACGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCACGCCACCt                                                                   >  6:212272/1‑139 (MQ=255)

                                                                                 ctcttcATCGAAGCTTCTGACAGGTGACTCTTCCGTTACACGCTGCATGGCTTCAATTGCCTTATACTCTGTTTTTTCAGCTTTAACAGCACCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCagaaaga                                               <  4:202173/138‑1 (MQ=255)

                                                                                           tAGCATCTGACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAg                                     >  4:364041/1‑139 (MQ=255)

                                                                                            aGCATCTGACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGc                                    >  7:429960/1‑139 (MQ=255)

                                                                                            aGCATATGACAGGATACTCTTCATTAACAAGCAGCAAGTCTTCAAATGACGTATACTCTCTATTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCACGCCACCTTAATGCCAAGCTCAGAAAGAATCtttt                                        >  6:90409/1‑135 (MQ=255)

                                                                                                tCTGACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGcta                                  >  5:279050/1‑135 (MQ=255)

                                                                                                tCTGACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGcta                                  >  3:56044/1‑135 (MQ=255)

                                                                                                   gACAGGAGACTCTTCCGTTACCCGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCAAATTTGCAACACGTGGCCAGCCACCTTAATGACAAGCTAAGAAAGAATCTTTTTAGCTACatcc                             >  8:22065/1‑136 (MQ=255)

                                                                                                   gACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGcc                             >  8:74225/1‑138 (MQ=255)

                                                                                                   gACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGcc                             >  5:439260/1‑138 (MQ=255)

                                                                                                   gACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGcc                             >  8:15602/1‑138 (MQ=255)

                                                                                                     caggAGACTCTTCCGTTACACGCTGCAAGTCTTCAAATGACGTATACTCTGTATATTCAGCAATAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGcccc                           <  3:142479/137‑1 (MQ=255)

                                                                                                        gagaCTCTTCCGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCGTAATGCCAAGCTCAGAAAGAATCTTGTTAGCTACAGCCCCTgc                        >  8:169235/1‑139 (MQ=255)

                                                                                                           aCTCTTCCGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTgccgc                     >  7:180844/1‑139 (MQ=255)

                                                                                                           aCTCTTCCGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGAAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTgccgc                     >  4:134128/1‑139 (MQ=255)

                                                                                                               ttccGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCAct                 >  1:181724/2‑139 (MQ=255)

                                                                                                                 ccGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCAc                  <  3:364041/136‑1 (MQ=255)

                                                                                                                  cGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCActct               <  7:74225/138‑1 (MQ=255)

                                                                                                                         cGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGc                                                                        >  2:439951/1‑74 (MQ=255)

                                                                                                                         cGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGc                                                                        <  1:439951/74‑1 (MQ=255)

                                                                                                                         cGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAgttg       >  3:184275/1‑139 (MQ=255)

                                                                                                                         cGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAgttg       >  6:218238/1‑139 (MQ=255)

                                                                                                                            tGCAAGTCTTCAATTGACGTTTACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCACGCCACCTTAAGGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCAACGCTGCCAgttgtt     >  8:278346/1‑138 (MQ=255)

                                                                                                                            tGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCCCTCTGACAGTTGttt    >  4:144643/1‑139 (MQ=255)

                                                                                                                            tGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAGTTGttt    >  2:49567/1‑139 (MQ=255)

                                                                                                                            tGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAGTTGttt    >  2:28854/1‑139 (MQ=255)

                                                                                                                              caagTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAGTTGTTTcc  >  2:80139/4‑139 (MQ=255)

GACAATGACTTCTACAATTCCGCCGATGGAATCTCCGTTGGCCTTCGCTTCATCAATGGCAGCCATCATTTTTTTGCCCGCTTCTTCATCGTAGCATCTGACCGGAGACTCCTCCGTTACACGCTGCAGGTCATCAATTGACGTATATTCTGTTTTTTCAGCTTTAACTGCGCCAATTTGCAATACGTGACCTGCCACCTTAATGCCGAGCTCAGAAAGAATCTTTTTAGCGACAGCTCCTGCCGCCACTCTGACAGTTGTTTCC  >  CP006881/2197466‑2197730

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A