Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A14 F56 I20 R2	1958	92.1	3575798	91.1%	3257551	126.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,197,787	T→A	G117G (GGA→GGT)	U712_11010 ←	Chorismate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,197,787	0	T	A	100.0%	122.5 / NA	43	G117G (GGA→GGT)	U712_11010	Chorismate synthase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (25/18); total (25/18)

CTGCCACCTTAATGCCGAGCTCAGAAAGAATCTTTTTAGCGACAGCTCCTGCCGCCACTCTGACAGTTGTTTCCCTTGCTGAAGAGCGCTCAAGCACATTTCTCATATCACGGTGATTATATTTAATCGCTCCGTTTAAGTCAGCGTGCCCAGGTCTCGGTCTGGAAATTTGGCGCTTCATTTCCTTTTCTTCATCCTCTGTAATCGGAGCCGCACCCATGATTTTTGTCCAATGCTTCCAATCGTTATTTTCAACGACGA  >  CP006881/2197657‑2197917

ccgCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAGTTGTTTCCCTTGCTGAAGAACGCTCAAGCACATTTCTCATATCACGATGATTATATTTAATTGCACCGTTTaa                                                                                                                            <  2:200984/137‑1 (MQ=255)

 cgCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAGTTGTTTCCCTTGCTGAAGAACGCTCAAGCACATTTCTCATATCACGATGATTATATTTAATTGCACCGTTTAAg                                                                                                                           <  5:290697/138‑1 (MQ=255)

    cACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAGTTGTTTCCCTTGCTGAAGAACGCTCAAGCACATTTCTCATATCACGATGATTATATTTAATTGCACCGTTTAAGt                                                                                                                          >  7:204382/1‑137 (MQ=255)

      ccTTAATGCCACGCTCCCCAAGAATCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAGTTGTGTCCCTTGCTGAAGAACGCGCAAGCACATTTCTCATATCACGATGATTATATTTAATTGCACCGTTTAAGTCAGc                                                                                                                      <  1:364140/139‑1 (MQ=255)

                 aGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCCCTCGGACAGTTGTTTCCCTTGCTGAAGCACGCTCAAGCACAATTCTCATATCACGAAGATTATATTTAATAGCACCGATTAAGTCAGCGTGcccgagtc                                                                                                           >  4:276453/1‑134 (MQ=255)

                 aGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAGTTGTTTCCCTTGCTGAAGAACGCTCAAGCACGTTTCTCATATCACGATGATTATATTTAATTGCACCGTTTAAGTCAGCGTGCCCAGGtc                                                                                                           >  6:263872/1‑139 (MQ=255)

                 aGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAGTTGTTTCCCTTGCTGAAGAACGCTCAAGCACATTTCTCATATCCCGATGATTATATTTAATTGCACCGTTTAAGTCAGCGTGCCCAGGtc                                                                                                           >  1:182076/1‑139 (MQ=255)

                 aGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAGTTGTTTCCCTTGCTGAAGAACGCTCAAGCACATTTCTCATATCACGATGATTATATTTAATTGCACCGTTTAAGTCAGCGTGCCCAGGtc                                                                                                           >  5:75606/1‑139 (MQ=255)

                 aGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAGTTGTTTCCCTTGCTGAAGAACGCTCAAGCACATTTCTCATATCACGATGATTATATTTAATTGCACCGTTTAAGTCAGCGTGCCCAGGtc                                                                                                           >  5:223694/1‑139 (MQ=255)

                  ccTCAGCAAGACTCTTTTTAGCGACAGCCCCTGCCGCCACTCTGACAGGTGTTTCCCTTGCAGAAGAACGCTCAAGCACATTTCTCATATCACGATGATTATATTTAATTGCACCGGTTAAGTCAGCGTGCCCAGGtct                                                                                                          <  2:184932/138‑1 (MQ=255)

                           gaaTCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAGTTGTTTCCCTTGCTGAAGAACGCTCAAGCACATTTCTCATATCACGATGATTATATTTAATTGCACCGTTTAAGTCAGCGTGCCCAGGTCTCGGTCTGGa                                                                                                 <  7:156886/139‑1 (MQ=255)

                           gaaTCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAGTTGTTTCCCTTGCTGAAGAACGCTCAAGCACATTTCTCATATCACGATGATTATATTTAATTGCACCGTTTAAGTCAGCGTGCCCAGGTCTCGGTCTGGa                                                                                                 <  3:397749/139‑1 (MQ=255)

                             aTCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAGTTGTTTCCCTTGCTGAAGAACGCTCAAGCACATTTCTCATATCACGATGATTATATTTAATTGCACCGTTTAAGTCa                                                                                                                        <  6:286975/114‑1 (MQ=255)

                             aTCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAGTTGTTTCCCTTGCTGAAGAACGCTCAAGCACATTTCTCATATCACGATGATTATATTTAATTGCACCGTTTAAGTCa                                                                                                                        >  5:286975/1‑114 (MQ=255)

                             aTCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAGTTGTTTCCCTATCTGAAGAACGCTCAAGCACATTTCTCATATCACGATGATTATATTTAATTGCACCGTTTAAGTCAGCGTGCCCAGGTCTCGGTCTGGaaa                                                                                               >  7:52533/1‑139 (MQ=255)

                                tttttAGCTCCAGCCAATTCCGCCACTCTGACAGTTGTTTCCGTTGCTTAAGAAAGCTCCAGCACATAAGTAATATCACGATGATTATATTTAATTGCACCGGTTAAGTCAGCGAGCCGAGGTCACGGTCTGGAAAtct                                                                                            >  2:295233/1‑137 (MQ=255)

                                                  gccgccACTCTGACAGTTGTTTCCCTTGCTGAAGAACGCTCAAGCACATTTCTCATATCACGATGATTATATTTAATTGCACCGTTTAAGTCAGCGTGCCCAGGTCTCGGTCTGGAAATCTGGCGCTTCATTTCCttt                                                                           >  6:398283/1‑138 (MQ=255)

                                                  gccgccACTCTGACAGTTGTTTCCCTTGCTGAAGAACGCTCAAGCACATTTCTCATATCACGATGAATATATTTAATTGCACCGTTTAAGTCAGCGTGCCCAGGTCTCGGTCTGGAAATCTGGCGCTTCATATCCtttt                                                                          >  7:338344/1‑139 (MQ=255)

                                                   ccgccACTCTGACAGTTGTTTCCCTTGCTGAAGAACGCTCAAGCACATTTCTCATATCACGATGATTATATTTAATTGCACCGTTTAAGTCAGCGTGCCCAGGTCTCGGTCTGGAAATCTGGCGCTTCATTTCCTTttc                                                                         <  1:214554/139‑1 (MQ=255)

                                                       cACTCTGACAGTTGTTTCCCTTGCTGAAGAACGCTCAAGCACATTTCTCATATCACGATGATTATATTTAATTGCACCGTTTAAGTCAGCGTGCCCAGGTCTCGGTCTGGAAATCTGGGGCGTCAGTTCCttttggtca                                                                     >  8:269881/1‑134 (MQ=255)

                                                          tctGACAGTTGTTTCCCTTGCTGAAGAACGCTCAAGCACATTTCTCATATCACGATGATTATATTTAATTGCACCGTTTAAGTCAGCGTGCCCAGGTCTCGGTCTGGAAATCTGGCGCTGCAGTTCCGTTTGTTAATca                                                                  >  2:406291/1‑138 (MQ=255)

                                                              aCAGTTGTTTCCCTTGCTGAAGAACGCTCAAGCACATTTCTCATATCACGATGATTATCTTTAATTGCACCGTTTAAGTCAGCGTGCCCAGGTCTCGGTCTGGAAATCTGGCGCTTCATTTCCTTTTCTTCATCttc                                                                <  1:406291/137‑1 (MQ=255)

                                                              aCAGTTGATTCCCTTGCTGAAGAACGCTCAAGCACATTTCTCATATCACGATGATTATATTTAATTGCACCGTTTAAGTCAGCGGGCCCAGGTCTCGGTCTGGAAATCTGGCGCTTCATTTCCTTTTCTTCAtcttct                                                               <  5:90409/138‑1 (MQ=255)

                                                                            tGCTGAAGAACGCTCAAGCACATTTCTCATATCACGATGATTATATTTAATTGCACCGTTTAAGTCAGCGTGCCCAGGTCTCGGTCTGGAAATCTGGCGCTTCATTTCCTTTTCTTCATCTTCTGTAATCGGAGCGGcg                                                >  3:183477/1‑138 (MQ=255)

                                                                            tGCTGAAGAACGCTCAAGCACATTTCTCATATCACGATGATTATATTTAATTGCACCGTTTAAGTCAGCGTGCCCAGGTCTCGGTCTGGAAATCTGGCGCTTCATTTCCTTTTCGTCATCTTCTGTAATCGGAGCGGcg                                                >  7:60014/1‑138 (MQ=255)

                                                                             gCTGAAGAACGCTCAAGCACATTTCTCATATCACGATGATTATATTTAATTGCACCGTTTAAGTCAGCGTGCCCAGGTCTCGGTCTGGAAATCTGGCGCTTCATTTCCTTTTCTTCATCTTCTGTAATCGGAGCGGcg                                                <  4:222091/138‑2 (MQ=255)

                                                                                      cgcTCAAGCACATTTCTCATATCACGATGATTATATTTAATTGCACCGTTTAAGTCAGCGTGCCCAGGTCTCGGTCTGGAAATCTGGCGCTTCATTTACTTTTGTTCATATTCTGTTATCGTAGCTGCGCCCATGAttt                                      >  7:322929/1‑139 (MQ=255)

                                                                                          cAAGCACATTTCTCATATCACGATGATTATATTTAATTGCACCGTTTAAGTCAGCGTGCCCAGGTCTCGGTCTGGAAATCTGGCGCTTCATTTCCTTTTCTTCATCTTCTGTAATCGGAGCGGCGCCCATGATTTTTGt                                  <  1:321058/139‑1 (MQ=255)

                                                                                           aaGCACATTTCTCATATCACGATGATTATATTTAATTGCACCGTTTAAGTCAGCGTGCCCAGGTCTCGGTCTGGAAATCTGGCGCTTCATTTCCTTTTCTTCAt                                                                    <  8:172680/104‑1 (MQ=255)

                                                                                           aaGCACATTTCTCATATCACGATGATTATATTTAATTGCACCGTTTAAGTCAGCGTGCCCAGGTCTCGGTCTGGAAATCTGGCGCTTCATTTCCTTTTCTTCAt                                                                    >  7:172680/1‑104 (MQ=255)

                                                                                           aaGCACATTTCTCATATCACGATGATTATATTTAATTGCACCGTTTAAGTCAGCGTGCCCAGGTCTCGGTCTGGAAATCTGGCGCTTCATTTCCTTTTCTTCATCTTCTGTAATCGGAGCGGCGCCCATGATTTTTg                                   <  8:180844/137‑1 (MQ=255)

                                                                                           aaGCACATTTCTCATATCACGATGATTATATTTAATTGCACCGTTTAAGTCAGCGTGCCCAGGTCTCGGTCTGGAAATCTGGCGCTTCATTTCCTTTTCTTCATCTTCTGTAATCGGAGCGGCGCCCATGATTTTTGTc                                 <  7:56492/139‑1 (MQ=255)

                                                                                              cacaTTTCTCCTATAAAGATGATTATATTTATTTGCAACGTTTAAGTCATAGTGCCCATGTCTCGGTCTTGAAAGCTGGAGCTTCATTTCCTTTTCTTCATATGCTGTAATCGGATCGGCGCCCATGATTTTTGTCCaa                              <  7:278346/139‑1 (MQ=255)

                                                                                                   ttCTCATATCACGATGATTATATTTAATTGCACCGTTTAAGTCAGCGTGCCCAGGtct                                                                                                          <  2:218247/58‑1 (MQ=255)

                                                                                                   ttCTCATATCACGATGATTATATTTAATTGCACCGTTTAAGTCAGCGTGCCCAGGtct                                                                                                          >  1:218247/1‑58 (MQ=255)

                                                                                                   ttCTCATATCACGATGATTATATTTAATTGCACCGTTTAAGTCAGCGTGCCCAGGTCTCGGTCTGGAAATCTGGCGCTTCAATTACTTTTCTTAATATTATGTAATAGGAGCGGCGCCCCATATTTTTGTCCTATgat                          >  4:77790/1‑136 (MQ=255)

                                                                                                   ttCTCATATCACGATGATTATATTTAATTGCACCGTTTAAGTCAGCGTGCCCAGGTCTCGGTCTGGAAATCTGGCGCGACTCTTCCTTTTCGTGATCATCTGTAATCGGAGCGGCGCCCCATATTTTTTTCCAAGGCt                          >  5:425864/1‑138 (MQ=255)

                                                                                                                atGATTATATTTAATTGCACCGTTTAAGTCAGCGTGCCCAGGTCTCGGTCTGGAAATCTGGCGCTTCATTTGCTTTTGTTCATCTTGTGTAATCGGAGCGGCGCCCATGTTTTTTGTGCAATGGTTGCAATCGTTAttt            >  4:168391/2‑139 (MQ=255)

                                                                                                                atGATTATATTTAATTGCACCGTTTAAGTCAGCGTGCCCAGGTCTCGGTCTGGAAATCTGGCGCTTCATTTCCTTTTCTTCAtcttct                                                               >  6:3225/2‑88 (MQ=255)

                                                                                                                atGATTATATTTAATTGCACCGTTTAAGTCAGCGTGCCCAGGTCTCGGTCTGGAAATCTGGCGCTTCATTTCCTTTTCTTCAtcttct                                                               <  5:3225/87‑1 (MQ=255)

                                                                                                                atGATTATATTTAATTGCACCGTTTAAGTCAGCGTGCCCAGGTCTCGGTCTGGAAATCTGGCGCTTAATTTCCTTTTCTTCGTCTTCTGTTATCGGAGCGGCGCCCATGATTTTTGTAGATTGCTTACAATCGTTAttt            >  8:75501/2‑139 (MQ=255)

                                                                                                                         tttAATTGCACCGTTTAAGTCAGCGTGCCCAGGTCTCGGTCTGGAAATCTGGCGCTTCATTTCCTTTTGTTCATCTTCTGTTATCGGAGCGGCGCCCATGATTTTTGTCCAATGCTTCCCATCGTTTTTTTCAactacg   >  5:75066/1‑139 (MQ=255)

                                                                                                                            aaTTGCACCGTTTAAGTCAGCGTGCCCAGGTCTCGGTCTGGAAATCTGGCGCTTCATTTCCTTTTCTTCATCTTCTTTAATCGGAGCGGCGCCCATGATTTTTGTCCAATGCTTCCAATCGTTATTTTCAActacga  >  6:314458/1‑137 (MQ=255)

CTGCCACCTTAATGCCGAGCTCAGAAAGAATCTTTTTAGCGACAGCTCCTGCCGCCACTCTGACAGTTGTTTCCCTTGCTGAAGAGCGCTCAAGCACATTTCTCATATCACGGTGATTATATTTAATCGCTCCGTTTAAGTCAGCGTGCCCAGGTCTCGGTCTGGAAATTTGGCGCTTCATTTCCTTTTCTTCATCCTCTGTAATCGGAGCCGCACCCATGATTTTTGTCCAATGCTTCCAATCGTTATTTTCAACGACGA  >  CP006881/2197657‑2197917

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A