Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A14 F56 I20 R2	1958	92.1	3575798	91.1%	3257551	126.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,197,949	A→G	R63R (CGT→CGC)	U712_11010 ←	Chorismate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,197,949	0	A	G	100.0%	146.7 / NA	46	R63R (CGT→CGC)	U712_11010	Chorismate synthase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (10/36); total (10/36)

GGTCTGGAAATTTGGCGCTTCATTTCCTTTTCTTCATCCTCTGTAATCGGAGCCGCACCCATGATTTTTGTCCAATGCTTCCAATCGTTATTTTCAACGACGAGAGCAATTGGTGAACCGAGTGTTCGTGCATGACGCACCCCGCTCATAATTTTGGCCTGGTCTTTTTCGATCTGCATGCGGCGGCCGCGGCCGT‑GGCCTTTTTGGCGTCTGGCAAGCTCAAAATTGATATCTTCCTCCGTTATGTAAAGCCCGGCAGGCACACC  >  CP006881/2197815‑2198080

ggTCTGGAAATCTGGCGCTTCATTTCCTTTTCTTCATCTTCTGTAATCGGAGCGGCGCCCATGATTTTTGTCCAATGCTTCCAATCGTTATTTTCAAATACGATAGCAATTTGCGAACCGAGTGTACGTTCCTGGCGCa                                                                                                                                  >  7:423394/1‑139 (MQ=255)

ggTCTGGAAATCTGGCGCTTCAGTTCCTTTTAGTGATCTTCTGTAATCGGAGAGGCGCCCATGATTTTTGTCAAATGCGTACAATCGTTATTTTCAACTACGATCGGAATTGGCGCCCCGAGTGTACGTGCCTGGCGCa                                                                                                                                  >  6:174228/1‑139 (MQ=255)

  tCTGGAAATCTGGCGCTTCATTTCCTTTTCTTCATCTTCTGTAATCGGAGCGGCGCCCATGATTTTTGTCCAATGCTTCCAATCGTTATTTTCAACTACGAGAGCAATTGGCGAACCGAGTGTACGTGCATGGCGCAcc                                                                                                                                <  3:68683/139‑1 (MQ=255)

        aaTCTGGCGCTTCATTTCCTTTTCTTCATCTTCTGTAATCGGAGCGGCGCCCATGATTTTTGTCCAATGCTTCCAATCGTTATTTTCAACTACGAGAGCAATTGGCGAACCGAGTGTACGTGCATGGCGCACCCCGCTc                                                                                                                          <  4:341121/139‑1 (MQ=255)

         atctGGCGCTTCATTTCCTTTTCTTCATCTTCTGTAATCGGAGCGGCGCCCATGATTTTTGTCCAATGCTTCCAATCGTTATTTTCAACTACGAGAGCAATTGGCGAACCGAGTGTACGTGCATGGCGCACCCCGCTCa                                                                                                                         <  3:144643/136‑1 (MQ=255)

         atctGGCGCTTCATTTCCTTTTCTTCATCTTCTGTAATCGGAGCGGCGCCCATGATTTTTGTCCAATGCTTCCAATCGTTATTTTCAACTACGAGAGCAATTGGCGAACCGAGTGTACGTGCATGGCGCACCCCGCTCa                                                                                                                         <  5:398283/136‑1 (MQ=255)

            tGGCGCTTCATTTCCTTTTCTTCATCTTCTGTAATCGGAGCGGCGCCCATGATTTTTGTCCAATGCTTCCAATCGTTATTTTCAACTACGAGAGCAATTGGCGAACCGAGTGTACGTGCATGGCGCACCCCGCTCATaa                                                                                                                      >  5:419096/1‑139 (MQ=255)

              gcgcTTCATTTGCTTTTGTTGATCTTCTGTAATCGGAGCGGCGCCCATGATTTTTGTGCCATGCTTCCAATCGTTATTTTCAACTACGAGAGCAATTTGCGAACCGAGTGTACGTGCATGGCGCACCCCGCTCATAAtt                                                                                                                    >  8:301247/1‑139 (MQ=255)

              gcgcTTCATTTCCTTTTCTTCATCTTCTGTAATCGGAGCGGCGCCCATGATTTTTGTCCAATGCTTCCAATCGTTATTTTCAACTACGAGAGCAATTGGCGAACCGAGTGTACGTGCATGGCGCACCCCGCTCATAAtt                                                                                                                    <  6:259902/139‑1 (MQ=255)

                     aTTTCCTTTTCTTCATCTTCTGTAATCGGAGCGGCGCCCATGATTTTTGTCCAATGCTTCCAATCGTTATTTTCAACTACGAGAGCAATTGGCGAACCGAGTGTACGTGCATGGCGCACCCCGCTCATAATTTTGGcc                                                                                                              <  6:223694/138‑1 (MQ=255)

                                   aTCTTCTGTAATCGGAGCGGCGCCCATGATTTTTGTCCAATGCTTCCAATCGTTATTTTCAACTACGAGAGCAATTGGCGAACCGAGTGTACGTGCATGGCGCACCCCGCTCATAATTTTGGCCTGGTCTTTTTCGAt                                                                                                <  1:177708/138‑1 (MQ=255)

                                     cttctGTAATCGGAGCGGCGCCCATGATTTTTGTCCAATGCTTCCAATCGTTATTTTCAACTACGAGAGCAATTGGCGAACCGAGTGTACGTGCATGGCGCACCCCGCTCATAATTTTGGCCTGGTCTTTTTCGATCTg                                                                                             >  3:284585/3‑139 (MQ=255)

                                         tGTAATCGGAGCGGCGCCCATGATTTTTGTCCAATGCTTCCAATCGTTATTTTCAACTACGAGAGCAATTGGCGAACCGAGTGTACGTGCATGGCGCACCCCGCTCATAATTTTGGGCTGGTCGTTTTCGATCTGCATg                                                                                         >  2:127216/1‑139 (MQ=255)

                                           tAATCGGAGCGGCGCCCATGATTTTTGTCCAATGCTTCCAATCGTTATTTTCAACTACGAGAGCAATTGGCGAACCGAGTGTACGTGCATGGCGCACCCCGCTCATAATTTTGGCCTGGTCTTTTTCGATCTGCATgcg                                                                                       <  1:23008/139‑1 (MQ=255)

                                                  aGCGGCGCCCATGATTTTTGTCCAATGCTTCCAATCGTTATTTTCAACTACGAGAGCAATTGGCGAACCGAGTGTACGTGCATGGCGCACCCCGCTCATAATTTTGGCCTGGTCTTTTTCGATCTGCATGCGgcggccg                                                                                >  3:400046/1‑139 (MQ=255)

                                                         cccATGATTTTTGTCCAATGCTTCCAATCGTTATTTTCAACTACGAGATCAATTGGCGAACCGAGTGTACGTGCATGGCGCACCCCGGTCATAATTTTGGCCTGGTCTTTTTCGATCTGCATGCGGCGGCCGCGGCCGt                                                                         <  6:425864/139‑1 (MQ=255)

                                                           cATGATTTTTGTCCAATGCTTCCAATCGTTATTTTCAACTACGAGAGCAATTGGCGAACCGAGTGTACGTGCATGGCGCACCCCGCTCATAATTTTGGCCTGGTCTTTTTCGATCTGCATGCGGCGGCCGCGGCCGT‑gt                                                                      <  6:75606/139‑2 (MQ=255)

                                                                      tCCAATGCTTCCAATCGTTATTTTCAACTACGAGAGCAATTGGCGAACCGAGTGTACGTGCATGGCGCACCCCGCTCATAATTTTGGCCTGGTCTTTTTCGATCTGCATGCGGCGGCCGCGGCCGTGGGCGTTTTTggg                                                            >  8:309277/1‑138 (MQ=255)

                                                                        cAATGCTTCCAATCGTTATTTTCAACTACGAGAGCAATTGGCGAACCGAGTGTACGTGCATGGCGCACCCCGCTCATAATTTTGGCCTGGTCTTTTTCGATCTGCATGCGGCGGCCGCGGCCGT‑GTCCTTTTTGGCGt                                                          <  7:75501/138‑1 (MQ=255)

                                                                             cTTCCAATCGTTATTTTCAACTACGAGAGCAATTGGCGAACCGAGTGTACGTGCATGGCGCACCCCGCTCATAATTTTGGCCTGGTCTTTTTCGATCTGCATGCGGCGGCCGCGGCCGT‑GTCCTTTTTGGCGTCTGGc                                                     <  3:5828/138‑1 (MQ=255)

                                                                                ccAATCGTTATTTTCAACTACGAGAGCAATTGGCGAACCGAGTGTACGTGCATGGCGCACCCCGCTCATAATTTTGGCCTGGTCTTTTTCGATCTGCATGCGGCGGCCGCGGCCGT‑GGGCGTTTTTGGGTCTgggcg                                                   >  6:177948/1‑134 (MQ=255)

                                                                                   aTCGTTATTTTCAACTACGAGAGCAATTGGCGAACCGAGTGTACGTGCATGGCGCACCCCGCTCATAATTTTGGCCTGGTCTTTTTCGATCTGCATGCGGCGGCCGCGGCCGT‑GTCCTTTTTGGCGTCTGGCAAGCTCa                                              <  3:168391/139‑1 (MQ=255)

                                                                                         aTTTTCAACTACGAGAGCAATTGGCGAACCGAGTGTACGTGCATGGCGCACCCCGCTCATAATTTTGGCCTGGTCTTTTTCGATCTGCATGCGGCGGCCGCGGCCGT‑GTCCTTTTTGGCGTCTGGCAAGCTCAAAATTg                                        <  3:251976/139‑1 (MQ=255)

                                                                                          ttttCAACTACGAGAGCAATTGGCGAACCGAGTGTACGTGCATGGCGCACCCCGCTCATAATTTTGGCCTGGGCTTTATCGATCTGCATGCGGCGGCCGCGGCCGT‑GTCCTTTTTGGCGGCTGGCAAGCTCAAAATTg                                        <  8:60014/138‑1 (MQ=255)

                                                                                                actacgaGAGCAATTGGCGAACCGAGTGTACGTGCATGGCGCACCCCGCTCATAATTTTGGCCTGGTCTTTTTCGATCTGCATGCGGCGGCCGCGGCCGT‑GTCCTTTTTGGCGTCTGGCAAGCTCAAAATTGATATCtt                                 <  1:76207/136‑1 (MQ=255)

                                                                                                 ctacgaGAGCAATTGGCGAACCTAGTGTCCGTGCATGGCGCACCCCGCTCATAATTTTGGCCTGGTCTTTTTCGATCTGCCTGCGGCGGCCGCGGCCGT‑GTCCTTTTTGGCGTCTGGCAAGCTCAAAATTGATATCTtc                                <  8:322929/137‑1 (MQ=255)

                                                                                                 ctacgaGAGCAATTGGCGAACCGAGTGTACGTGCATGGCGCACCCCGCTCATAATTTTGGCCTGGTGTTTTTCGATCTGCATGCGGCGGCCGCGGCCGT‑GTAGTTTTTGGAGTCTGGCAAGCTCAAAATTGATATCTtc                                <  1:295233/137‑1 (MQ=255)

                                                                                                 ctacgaGAGCAATTGGCGAACCGAGTGTACGTGCATGGCGCACCCCGCTCATAATTTTGGCCTGGTCTTTTTCGATCTGCATGCGGCGGCCGCGGCCGT‑GTCCTTTTTGGCGTCTGGCAAGCTCAAAATTGATATCTtc                                <  5:314458/137‑1 (MQ=255)

                                                                                                      agagCAATTGGCGAACCGAGTGTACGTGCATGGCGCACCCCGCTCATAATTTTGGCCTGGTCTTTTTCGATCTGCATGCGGCGGCCGCGGCCGT‑GTCCTTTTTGGCGTCTGGCAAGCTCAAAATTGATATCTTCCTCCg                           <  8:423394/139‑1 (MQ=255)

                                                                                                      agagCAATTGGCGAACCGAGTGTACGTGCATGGCGCACCCCGCTCATAATTTTGGCCTGGTCTTTTTCGATCTGCATGCGGCGGCCGCGGCCGT‑GTCCTTTTTGGCGTCTGGCAAGCTCAAAATTGATATCTTCCTCCg                           <  4:400046/139‑1 (MQ=255)

                                                                                                            aTTGGCGAACCGAGTGTACGTGCATGGCGCACCCCGCTCATAATTTTGGCCTGGTCTTTTTCGATCTGCATGCGGCGGCCGCGGCCGT‑GTCCTTTTTGGCGTCTGGCAAGCTCAAAATTGATATCTTCCTCCGTTATGt                     <  3:276453/139‑1 (MQ=255)

                                                                                                             ttGGCGAACCGCGTGTACGTGACTGGCGCACCCCGCTCATAATTTTTGCCTGGTCTTTTTCTATCTGCATGCGGCGGCCGCGGCCGT‑GTCCTTTTTGTCGTCTGGGAAGCTCAAAATTGATATCTTCCTCCGTTATGTa                    <  6:436314/139‑1 (MQ=255)

                                                                                                                 cgAACCGAGTGTACGTGCATGGCGCACCCCGCTCATAATTTTGGCCTGGTCTTTTTCGTTCTGCATGCGGCGGCCGCGGCCGT‑GTCCTTTTTGGCGTCTGGCAAGCTCAAAATTGATATCTTCCTCCGTTATGTAAAGc                <  2:82333/138‑1 (MQ=255)

                                                                                                                 cgAACCGAGTGTACGTGCATGGCGCACCCCGCTCATAATTTTGGCCTGGTCTTTTTCGATCTGCATGCGGCGGCCGCGGCCGT‑GTCCTTTTTGGCGTCTGGCAAGCTCAAAATTGATATCTTCCTCCGTTATGTAAAGc                <  4:284585/138‑1 (MQ=255)

                                                                                                                     ccGAGTGTACGTGCATGGCGCACCCCGCTCATAATTATGGCCTGGGCTTTTTCGATCTGCATGCGGCGGCCGCGGCCGT‑GTCCTTTTTGGCGTCTGGCAAGCTCAAAATTGATATCTTCCTCCGTTATGTAAAGCCCgg            <  8:204382/139‑1 (MQ=255)

                                                                                                                     ccGAGTGGACGTGCATGGCTCAGCCCGCTCATAATTTTGGCCTGGTCTTTTTCGATCTGCATGCGGCGGCCGCGGCCGT‑GTCCTTTTTGGCGTCTGGCAAGCTCAAAATTGATATCTTCCTCCGTTATGTAAAGCCCgg            <  6:71953/139‑1 (MQ=255)

                                                                                                                        aGTGTACGTGCATGGCGCACCCCGCGCATAATTTTGGCCTGGTCTTTTTCGATCTGCATGCGGCGGCCGCGGCTGT‑GTCCTTTTTGGCGTCTGGCAAGCTCAAAATTGATATCTTCCTCCGTTATGTAAAGCCCggcag         >  7:62843/1‑139 (MQ=255)

                                                                                                                              cGTGCATGGCGCACCCCGCTCATAATTTTGGCCTGGTCTTTTTCGATCTGCTTGCGGCGGCCGCGGCCGT‑GTCCTTTTTGGCGTCTGGCAAGCTCAAAATTGATATCTTCCTCCGTTATGTATAGCCCGGCAGGtaca    <  3:77790/138‑1 (MQ=255)

                                                                                                                               gTGCATGGCGCACCCCGCTCATAATTTTGGCCTGGTCTTTTTCGATCTGCATGCGGCGGCCGCGGCTGT‑GTCCTTTTTGGCGTCTGGCAAGCTCAAAATTGATATCTTCCTCCGTTATGTAAAGCCCGGCAGGTACAcc  <  8:62843/139‑1 (MQ=255)

                                                                                                                               gTGCATGGCGCACCCCGCTCATAATTTTGGCCTGGTCTTTTTCGATCTGCATGCGGCGGCCGCGGCCGT‑GTCCTTTTTGGCGTCTGGCAAGCTCAAAATTGATATCTTCCTCCGTTATGTAAAGCCCGGCAGGTACAcc  <  7:399963/139‑1 (MQ=255)

                                                                                                                               gTGCATGGCGCACCCCGCTCATAATTTTGGCCTGGTCTTTTTCGATCTGCATGCGGCGGCCGCGGCCGT‑GTCCTTTTTGGCGTCTGGCAAGCTCAAAATTGATATCTTCCTCCGTTATGTAAAGCCCGGCAGGTACAcc  <  7:309277/139‑1 (MQ=255)

                                                                                                                               gTGCATGGCGCACCCCGCTCATAATTTTGGCCTGGTCTTTTTCGATCTGCATGCGGCGGCCGCGGCCGT‑GTCCTTTTTGGCGTCTGGCAAGCTCAAAATTGATATCTTCCTCCGTTATGTAAAGCCCGGCAGGTACAcc  <  5:177948/139‑1 (MQ=255)

                                                                                                                               gTGCATGGCGCACCCCGCTCATAATTTTGGCCTGGTCTTTTTCGATCTGCATGCGGCGGCCGCGGCCGT‑GTCCTTTTTGGCGTCTGGCAAGCTCAAAATTGATATCTTCCTCCGTTATGTAAAGCCCGGCAGGTACAcc  <  4:43522/139‑1 (MQ=255)

                                                                                                                               gTGCATGGCGCACCCCGCTCATAATTTTGGCCTGGTCTTTTTCGATCTGCATGCGGCGGCCGCGGCCGT‑GTCCTTTTTGGCGTCTGGCAAGCTCAAAATTGATATCTTCCTCCGTTATGTAAAGCCCGGCAGGTACAcc  <  4:26423/139‑1 (MQ=255)

                                                                                                                               gTGCATGGCGCACCCCGCTCATAATTTTGGCCAGGTCTTTTTCGATCTGCATGCGGCGGCCGCGGCCGT‑GTCCTTTTTGGCGTCTGGCAAGCTCAAAATTGATATCTTCCTCCGTTATGTAAAGCCCGGCAGGTACAcc  <  5:79071/139‑1 (MQ=255)

                                                                                                                               aggCATGGCGCACCCCGCTCTTAATTTTGGCCTGGTCTTTTTCGATGTGCTTGCGGCGGCCGCGGCCGT‑GTCCTTTTTGGCGTCTGGCAAGCTCAAAATTGATATCTTCCTCCGTTATGTAAAGCCCGGCAGGTACAcc  <  1:405834/137‑1 (MQ=255)

GGTCTGGAAATTTGGCGCTTCATTTCCTTTTCTTCATCCTCTGTAATCGGAGCCGCACCCATGATTTTTGTCCAATGCTTCCAATCGTTATTTTCAACGACGAGAGCAATTGGTGAACCGAGTGTTCGTGCATGACGCACCCCGCTCATAATTTTGGCCTGGTCTTTTTCGATCTGCATGCGGCGGCCGCGGCCGT‑GGCCTTTTTGGCGTCTGGCAAGCTCAAAATTGATATCTTCCTCCGTTATGTAAAGCCCGGCAGGCACACC  >  CP006881/2197815‑2198080

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A