Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A14 F56 I20 R2
|
1958 |
92.1 |
3575798 |
91.1% |
3257551 |
126.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
CP006881 |
2,198,245 |
2 bp→GA |
coding (737‑738/771 nt) |
U712_11015 ← |
Chemotaxis protein methyltransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | CP006881 | 2,198,245 | 0 | A | G | 98.0%
| 154.3
/ NA
| 49 | A246V (GCT→GTC) | U712_11015 | Chemotaxis protein methyltransferase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (23/25); minor base T (1/0); total (24/25) |
| Fisher's exact test for biased strand distribution p-value = 4.90e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
| * | CP006881 | 2,198,246 | 0 | G | A | 98.0%
| 145.6
/ NA
| 49 | A246V (GCT→GTC) | U712_11015 | Chemotaxis protein methyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (24/24); minor base T (0/1); total (24/25) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CGGCTGTTAAATATCTCATGACTTCTTCTCCCTTCATCGCATTAACGCTTTTAAGTAAAACTACTATAACATATTTTTCAGCAAAAAGTCAGTCTATCTTTTTTGATAAAAGAATGTATCGGCTGGAGCCAGGCCAAATTTCTCCGGATTGAAGATTTGCTCGGTGCTGCCGACAAATAGGACTCCATTCTTTTTCAAGCTATGTGCCATTTTTAAATAAAGCTCTTCCTTGGCGCTTTCTGTAAAA > CP006881/2198119‑2198365
||
cGGCTGTTAAATATCTCATGACTTCTTCTCCCTTCATCGCATTAACGCTTTTAAGTAAGACTACTATAACATATTTTTCAGCAAAAAGTCAGTCTATCTTTTTTGATAAAAGAATGTATCGGCTGGGACTAGGCCAAAt > 8:282002/1‑139 (MQ=255)
gCTGTTAAATATCTCATGACTTCTTCTCCCTTCATCGCATTAACGCTTTTAAGTAAGACTACTATAACATATTTTTCAGCAAAAAGTCAGTCTATCTTTTTTGATAAAAGAATGTATCGGCTGGGACTAGGCCAAAtt > 1:178772/1‑138 (MQ=255)
tGTTAAATATCTCATGACTTCTACACCCTTCATCGCATTAACGCTTTTAAGTAAGACTACTATAACATATTTTTAAGCAAAAAGTCAGACTAGCTTTTTTGATAAAAGAAAGTATCTGATGGTACAACGCAAAATTtct > 8:405160/1‑139 (MQ=255)
tctcATGACTTCTTCTCCCTTCATCGCATTAACGCTTTTAAGTAAGACTACTATAACATATTTTTCAGCAAAAAGTCAGTCTATCTTTTTTGATAAAAGAATGTATCGGCTGGGACTAGGCCAAATTTCTCGGGATTg < 4:379362/138‑1 (MQ=255)
cATGACTTCTTCTCCCTTCATCGCATTAACGCTTTTAAGTAAGACTACTATAACATATTTTTCAGCAAAAAGTCAGTCTATCTTTTTTGATAAAAGAATGTATCGGCTGGGACTAGGCCAAATTTCTCGGGATTg > 2:134173/1‑135 (MQ=255)
cttctCCCTTCATCGCATTAACGCTTTTAAGTAAGACTACTATAACATATTTTTCAGCAAAAAGTCAGTCTATCTTTTTTGATAAAAGAATGTATCGGCTGGGACTAGGCCAAATTTCTCGGGATTGAAGATTTGCt < 2:178772/137‑1 (MQ=255)
cttctCCCTTCATCGCATTAACGCTTTTAAGTAAGACTACTATAACATATTTTTCAGCAAAAAGTCAGTCTATCTTTTTTGATAAAAGAATGTATCGGCTGGGACTAGGCCAAATTTCTCGGGATTGAAGATTTGCTCg > 8:239346/1‑139 (MQ=255)
cttctCCATTCATCGCATTAACGCTTTTAAGTAAGACTACTATAACATATTTTTCAGCAAAAAGTCAGTCTATCTTTTTTGATAAAAGAATGTATCGGCTGGGACTAGGCCAAATTTCTCGGGATTGAAGATTTGCt < 5:28367/137‑1 (MQ=255)
ttctCCCTTCATCGCATTAACGCTTTTAAGTAAGACTACTATAACATATTTTTCAGCAAAAAGTCAGTCTATCTTTTTTGATAAAAGAATGTATCGGCTGGGACTAGGCCAAATTTCTCGGGATTGAAGATTTGCTCgg > 6:313704/1‑139 (MQ=255)
ttCATCGCATTAACGCTTTTAAGTAAGACTACTATAACATATTTTTCAGCAAAAAGTCAGTCTATCTTTTTTGATAAAAGAATGTATCGGCTGGGACTAGGCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGcc > 7:154119/1‑139 (MQ=255)
cATTAACGCTTTTAAGTAAGACTACTATAACATATTTTTCAGCAAAAAGTCAGTCTATCTTTTTTGATAAAAGAATGTATCGGCTGGGACTAGGCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACaaaa < 7:282002/139‑2 (MQ=255)
ttAAGTAAGACTACTATAACATATTTTTCAGCAAAAAGTCAGTCTATCTTTTTTGATAAAAGAATGTATCGGCTGGGACTAGGCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCAtt > 1:433449/1‑139 (MQ=255)
ttAAGTAAGACTACTATAACATATTTTTCAGCAAAAAGTCAGTCTATCTTTTTTGATAAAAGAATGTATCGGCTGGGACTAGGCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCAtt > 2:29975/1‑139 (MQ=255)
ttAAGTAAGACTACTATAACATATTTTTCAGCAAAAAGTCAGTCTATCTTTTTTGATAAAAGAATGTATCGGCTGGGACTAGGCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCAtt > 5:153629/1‑139 (MQ=255)
taagtaagTCTATTTTAACATATTTTTCAGAATAAAGTCAGTATTTCTTTTTTGATAAAAGAATGTAGCGGCTGGGTCTAGGCCATATTTCTGGGGATTGAAGATTTGCTCGGTGCTGCCGACAAATAGAACTGCAtt < 5:200524/138‑1 (MQ=255)
taagtaagACTACTATAACATATTTTTCAGCAAAAAGTCAGTCTATCTTTTTTGATAAAAGAATGTATCGGCTGGGACTAGGCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCAtt < 1:231756/138‑1 (MQ=255)
taagtaagACTACTATAACATATTTTTCAGCAAAAAGTCAGTCTATCTTTTTTGATAAAAGAATGTATCGGCTGGGACTAGGCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCAtt < 8:154119/138‑1 (MQ=255)
tAATTAAGACTACTATAACATATTTTTCAGCAAAAAGTCAGTCTATCTTTTTTGATAAAAGAATGTATCGGCTGGGACTAGGCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCAtt < 8:357518/138‑1 (MQ=255)
agactactATAACATATTTTTCAGCAAAAAGTCAGTCTATCTTTTTTGATAAAAGAATGTATCGGCTGGGACTAGGCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCATTcttctt > 5:412621/3‑138 (MQ=255)
gactactATAACATATTTTTCAGCAAAAAGTCAGTCTATCTTTTTTGATAAAAGAATGTATCGGCTGGGACTAGGCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCAttcttcttc > 1:105339/2‑138 (MQ=255)
gactactATAACATATTTTTCAGCAAAAAGTCAGTCTATCTTTTTTGATAAAAGAATGTATCGGCTGGGACTAGGCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCATTCTTCTTCa < 5:364503/138‑1 (MQ=255)
ctaTAACATATTTTTCAGCAAAAAGTCAGTCTATCTTTTTTGATAAAAGAATGTATCGGCTGGGACTAGGCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAgaa > 2:358282/1‑117 (MQ=255)
ctaTAACATATTTTTCAGCAAAAAGTCAGTCTATCTTTTTTGATAAAAGAATGTATCGGCTGGGACTAGGCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAgaa < 1:358282/119‑3 (MQ=255)
ataACATATTTTTCAGCAAAAAGTCAGTCTATCTTTTTTGATAAAAGAATGTATCGGCTGGGACTAGGCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCATTCTTCTTCAAGCTAtg > 3:118983/1‑139 (MQ=255)
cATATTTTTCAGCAAAAAGTCAGTCTATCTTTTTTGATAAAAGAATGTATCGGCTGGGACTAGGCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCATTCTTCTTCAAGCTATGTGc > 5:380173/1‑138 (MQ=255)
cATATTTTTCAGCAAAAAGTCAGTCTATCTTTTTTGATAAAAGAATGTATCGGCTGGGACTAGGCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCATTCTTCTTCAAGCTATGTGc > 8:43251/1‑138 (MQ=255)
cATATTTTTCAGCAAAAAGTCAGTCTATCTTTTTTGATAAAAGAATGTATCGGCTGGGACTAGGCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCATTCTTCTTCAAGCTATGTGc > 1:81286/1‑138 (MQ=255)
atatTTTTCAGCAAAAAGTCAGTCTATCTTTTTTGATAAAAGAATGTATCGGCTGGGACTAGGCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCATTCTTCTTCAAGCTATGTGCCa < 8:326692/139‑1 (MQ=255)
atatTTTTCAGCAAAAAGTCAGTCTATCTTTTTTGATAAAAGAATGTATCGGCTGGGACTAGGCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCATTCTTCTTCAAGCTATGTGCCa < 7:343875/139‑1 (MQ=255)
atatTTTTCAGCAAAAAGTCAGTCTATCTTTTTTGATAAAAGAATGTATCGGCTGGGACTAGGCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCATTCTTCTTCAAGCTATGTGCCa < 7:243000/139‑1 (MQ=255)
tatTTTTCAGCAAAAAGTCAGTCTATCTTTTTTGATAAAAGAATGTATCGGCTGGGACTAGGCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCATTCTTCTTCAAGCTATGTGCCAt > 1:26750/1‑139 (MQ=255)
tatTTTTCAGCAAAAAGTCAGTCTATCTTTTTTGATAAAAGAATGTATCGGCTGGGACTAGGCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCATTCTTCTTCAAGCTATGTGCCAt > 8:112268/1‑139 (MQ=255)
ttttCAGCAAAAAGTCAGTCTATCTTTTTTGATAAAAGAATGTATCGGCTGGGACTAGGCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCATTCTTCTTCAAGCTATGTGCCAtttt > 1:347858/1‑139 (MQ=255)
tttCAGCAAAAAGTCAGTCTATCTTTTTTGATAAAAGAATGTATCGGCTGGGACTAGGCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACaaaaa > 4:268857/1‑102 (MQ=255)
tttCAGCAAAAAGTCAGTCTATCTTTTTTGATAAAAGAATGTATCGGCTGGGACTAGGCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACaaaaa < 3:268857/104‑3 (MQ=255)
ttCAGCAAAAAGTCAGTCTATCTTTTTTGATAAAAGAATGTATCGGCTGGGACTAGGCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCATTCTTCTTCAAGCTATGTGCCAttttt < 6:380173/138‑1 (MQ=255)
aGCAAAAAGTCAGTCTATCTTTTTTGATAAAAGAATGTATCGGCTGGGACTAGGCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCATTCTTCTTCAAGCTATGTGCCATTTTtaaa < 5:103692/138‑1 (MQ=255)
aGCAAAAAGTCAGTCTATCTTTTTTGATAAAAGAATGTATCGGCTGGGACTAGGCCAAATTTCTCGGGATTGAAGATTTGATCGGTGCTGCCGACAAAAAGAACTCCATTCTTCTTCAAGCTATGTGCCATTTTtaaa > 6:103692/1‑138 (MQ=255)
agtcagtcTATCTTTTTTGATAAAAGAATGTATCGGCTGGGACTAGGCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCATTCTTCTTCAAGCTATGTGCCATTTTTAAATACAGctc < 6:368623/139‑1 (MQ=255)
agtcagtcTATCTTTTTTGATAAAAGAATGTATCGGCTGGGACTAGGCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCATTCTTCTTCAAGCTATGTGCCATTTTTAAATACAGctc < 5:313704/139‑1 (MQ=255)
agtcagtcTATCTTTTTTGATAAAAGAATGTATCGGCTGGGACTAGGCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCATTCTTCTTCAAGCTATGTGCCATTTTTAAATACAGctc < 1:134173/139‑1 (MQ=255)
gtcTATCTTTTTTGATAAAAGAATGTATCGGCTGGGACTAGGCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCATTCTTCTTCAAGCTATGTGCCATTTTTAAATACAGCTCTTCCt > 1:406119/1‑139 (MQ=255)
ttttGATAAAAGAATGTATCGGCTGGGACTAGGCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCATTCTTCTTCAAGCTATGTGCCATTTTTAAATACAGCTCTTCCTTCGCGCt < 3:168790/137‑1 (MQ=255)
ttttGATAAAAGAATGTATCGGCTGGGACTAGGCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCATTCTTCTTCAAGCTATGTGCCATTTTTAAATACAGCTCTTCCTTCGCGCt < 4:118983/137‑1 (MQ=255)
ttttGATAAAAGAATGTATCGGCTGGGACTAGGCCAAATTTATCGGGATTGAAGATTTGCTCGGTGCTGCCCACAAAAAGAACTCCATTCTTCTTCAAGCTATGTGCCATTTTTAAATACAGCTCTTCCTTCGCGCttt > 7:123737/1‑139 (MQ=255)
aTAAAAGAATGTATCGGCTGGGACTAGGCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCATTCTTCTTCAAGCTATGTGCCATTTTTAAATACAGCTCTTCCTTCGCGCTTTCTGTa < 6:412621/139‑1 (MQ=255)
aaaaaaTGTATCGGCTGGGACTAGGCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCATTCTTCTTCAAGCTATGTGCCATTTTTAAATACAGCTCTTCCTTCGCGCTTTCTGTaaaa < 6:177379/139‑1 (MQ=255)
aaaGAATGTATCGGCTGGGACTAGGCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCATTCTTCTTCAAGCTATGTGCCATTTTTAAATACAGCTCTTCCTTCGCGCTTTCTGTaaaa < 6:153629/139‑1 (MQ=255)
aaaGAATGTATAGGCTGGGACTAGGCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCATTCTTCTTCAAGCTATGTGCCATTTTTAAATACAGCTCTTCCTTCGCGCTTTCTGTaaaa < 2:433449/139‑1 (MQ=255)
||
CGGCTGTTAAATATCTCATGACTTCTTCTCCCTTCATCGCATTAACGCTTTTAAGTAAAACTACTATAACATATTTTTCAGCAAAAAGTCAGTCTATCTTTTTTGATAAAAGAATGTATCGGCTGGAGCCAGGCCAAATTTCTCCGGATTGAAGATTTGCTCGGTGCTGCCGACAAATAGGACTCCATTCTTTTTCAAGCTATGTGCCATTTTTAAATAAAGCTCTTCCTTGGCGCTTTCTGTAAAA > CP006881/2198119‑2198365
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
