Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A14 F56 I20 R2 1958 92.1 3575798 91.1% 3257551 126.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 2,198,338 A→C L215L (CTT→CTG)  U712_11015 ← Chemotaxis protein methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,198,3380AC100.0% 176.1 / NA 54L215L (CTT→CTG) U712_11015Chemotaxis protein methyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (29/25);  total (29/25)

AGTCAGTCTATCTTTTTTGATAAAAGAATGTATCGGCTGGAGCCAGGCCAAATTTCTCCGGATTGAAGATTTGCTCGGTGCTGCCGACAAATAGGACTCCATTCTTTTTCAAGCTATGTGCCATTTTTAAATAAAGCTCTTCCTTGGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACAATTAAGTCAAAGTCTTGTTCATAACGATCAGCCAGTAGATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGCTTTTA  >  CP006881/2198205‑2198471
                                                                                                                                     |                                                                                                                                     
agtcagtcTATCTTTTTTGATAAAAGAATGTATCGGCTGGGACTAGGCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCATTCTTCTTCAAGCTATGTGCCATTTTTAAATACAGctc                                                                                                                                  <  1:134173/139‑1 (MQ=255)
agtcagtcTATCTTTTTTGATAAAAGAATGTATCGGCTGGGACTAGGCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCATTCTTCTTCAAGCTATGTGCCATTTTTAAATACAGctc                                                                                                                                  <  5:313704/139‑1 (MQ=255)
agtcagtcTATCTTTTTTGATAAAAGAATGTATCGGCTGGGACTAGGCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCATTCTTCTTCAAGCTATGTGCCATTTTTAAATACAGctc                                                                                                                                  <  6:368623/139‑1 (MQ=255)
     gtcTATCTTTTTTGATAAAAGAATGTATCGGCTGGGACTAGGCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCATTCTTCTTCAAGCTATGTGCCATTTTTAAATACAGCTCTTCCt                                                                                                                             >  1:406119/1‑139 (MQ=255)
              ttttGATAAAAGAATGTATCGGCTGGGACTAGGCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCATTCTTCTTCAAGCTATGTGCCATTTTTAAATACAGCTCTTCCTTCGCGCt                                                                                                                      <  3:168790/137‑1 (MQ=255)
              ttttGATAAAAGAATGTATCGGCTGGGACTAGGCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCATTCTTCTTCAAGCTATGTGCCATTTTTAAATACAGCTCTTCCTTCGCGCt                                                                                                                      <  4:118983/137‑1 (MQ=255)
              ttttGATAAAAGAATGTATCGGCTGGGACTAGGCCAAATTTATCGGGATTGAAGATTTGCTCGGTGCTGCCCACAAAAAGAACTCCATTCTTCTTCAAGCTATGTGCCATTTTTAAATACAGCTCTTCCTTCGCGCttt                                                                                                                    >  7:123737/1‑139 (MQ=255)
                   aTAAAAGAATGTATCGGCTGGGACTAGGCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCATTCTTCTTCAAGCTATGTGCCATTTTTAAATACAGCTCTTCCTTCGCGCTTTCTGTa                                                                                                               <  6:412621/139‑1 (MQ=255)
                      aaaaaaTGTATCGGCTGGGACTAGGCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCATTCTTCTTCAAGCTATGTGCCATTTTTAAATACAGCTCTTCCTTCGCGCTTTCTGTaaaa                                                                                                            <  6:177379/139‑1 (MQ=255)
                      aaaGAATGTATCGGCTGGGACTAGGCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCATTCTTCTTCAAGCTATGTGCCATTTTTAAATACAGCTCTTCCTTCGCGCTTTCTGTaaaa                                                                                                            <  6:153629/139‑1 (MQ=255)
                      aaaGAATGTATAGGCTGGGACTAGGCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCATTCTTCTTCAAGCTATGTGCCATTTTTAAATACAGCTCTTCCTTCGCGCTTTCTGTaaaa                                                                                                            <  2:433449/139‑1 (MQ=255)
                                        gactaGGCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCATTCTTCTTCAAGCTATGTGCCATTTTTAAATACAGCTCTTCCTTCGCGCTTTCTGTaaaa                                                                                                            <  6:366412/117‑1 (MQ=255)
                                        gactaGGCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCATTCTTCTTCAAGCTATGTGCCATTTTTAAATACAGCTCTTCCTTCGCGCTTTCTGTaaaa                                                                                                            >  5:366412/5‑121 (MQ=255)
                                        gactaGGCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCATTCTTCTTCAAGCTATGTGCCATTTTTAAATACAGCTCTTCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCgg                                                                                          >  3:376529/5‑139 (MQ=255)
                                          ctaGGCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCATTCTTCTTCAAGCTATGTGCCATTTTTAAATACAGCTCTTCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGca                                                                                        <  1:29975/137‑1 (MQ=255)
                                             ggCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCATTCTTCTTCAAGCTATGTGCCATTTTTAAATACAGCTCTTCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGcacac                                                                                     >  7:201869/1‑139 (MQ=255)
                                             ggCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCATTCTTCTTCAAGCTATGTGCCATTTTTAAATACAGCTCTTCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGcacac                                                                                     >  4:115067/1‑139 (MQ=255)
                                             ggCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCATTCTTCTTCAAGCTATGTGCCATTTTTAAATACAGCTCTTCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGcac                                                                                       <  7:43251/137‑1 (MQ=255)
                                             ggCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCATTCTTCTTCAAGCTATGTGCCATTTTTAAATACAGCACTTCCTTCGCGCTTTCTGTAAAATAAATAAAAACATTCCGGcacac                                                                                     >  2:252732/1‑139 (MQ=255)
                                              gCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCATTCTTCTTCAAGCTATGTGCCATTTTTAAATACAGCTCTTCCTTCGCGCTTTCTGTAAAATAAATAATTACATTGCGGcacac                                                                                     >  4:31594/1‑138 (MQ=255)
                                              gCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCATTCTTCTTCAAGCTATGTGCCATTTTTAAATACAGCTCTTCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGcacac                                                                                     >  2:253269/1‑138 (MQ=255)
                                                  aaTTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCATTCTTCTTCAAGCTATGTGCCATTTTTAAATACAGCTCTTCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACGAtt                                                                                 >  5:108278/1‑138 (MQ=255)
                                                       ctcGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTACATTCTTCTTCAAGCTATGTGCCAGTTTTAAATACAGCTCTTCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACGATTAAGTc                                                                            >  7:8559/1‑138 (MQ=255)
                                                          gggATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCATTCTTCTTCAAGCTATGTGCCATTTTTAAATACAGCTCTTCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACGATTAAGTCa                                                                           <  2:26750/135‑1 (MQ=255)
                                                            gATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCATTCTTCTTCAAGCTATGTGCCATTTTTAAATACAGCTCTTCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACGATTAAGTCAAAGTc                                                                      <  2:39613/139‑1 (MQ=255)
                                                            gATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCATTCTTCTTCAAGCTATGTGCCATTTTTAAATACAGCTCTTCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACGATTAAGTCAAAGTc                                                                      <  3:115067/139‑1 (MQ=255)
                                                            gATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCATTCTTCTTCAAGCTATGTGCCATTTTTAAATACAGCTCTTCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACGATTAAGTCAAAGTc                                                                      <  2:105339/139‑1 (MQ=255)
                                                            gATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCATTCTTCTTCAAGCTATGTGCCATTTTTAAATACAGCTCTTCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACGATTAAGTCAAAGTc                                                                      <  2:347858/139‑1 (MQ=255)
                                                            gATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCATTCTTCTTCAAGCTATGTGCCATTTTTAAATACAGCTCTTCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACGATTAAGTCAAAGTc                                                                      <  5:421213/139‑1 (MQ=255)
                                                                 aaGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCATTCTTCTTCAAGCTATGTGCCATTTTTAAATACAGCTCTTCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACGATTAAGTCAAAGTCTTGtt                                                                 >  1:133478/1‑139 (MQ=255)
                                                                 aaGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCATTCTTCTTCAAGCTATGTGCCATTTTTAAATACAGCTCTTCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACGATTAAGTCAAAGTCTTGt                                                                  <  1:253269/138‑1 (MQ=255)
                                                                      ttGCTCGGTGCTGCCGACAAAAAGAACTCCATTCTTCTTCAAGCTATGTGCCATTTTTAAATACAGCTCTTCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATaa                                                            >  3:305137/1‑139 (MQ=255)
                                                                      ttGCTCGGTGCTGCCGACAAAAAGAACTCCATTCTTCTTCAAGCTATGTGCCATTTTTAAATACAGCTCTTCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATaa                                                            >  7:125756/1‑139 (MQ=255)
                                                                       tGCTCGGTGCTGCCGACAAAAAGAACTCCATTCTTCTTCAAGCTATGTGCCATTTTTAAATACAGCTCTTCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATaa                                                            <  8:125756/138‑1 (MQ=255)
                                                                               gctgcCGACAAAAAGAACTCCATTCTTCTTCAAGCTATGTGCCATTTTTAAATTCAGCTCTTCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGcc                                                   <  7:112268/139‑1 (MQ=255)
                                                                                ctgcCGACAAAAAGAACTCCATTCTTCTTCAAGCTATGTGCCATTTTTAAATACAGCTCTTCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCa                                                  <  7:239346/139‑1 (MQ=255)
                                                                                   ccGACAAAAAGAACTCCATTCTTCTTCAAGCTATGTGCCATTTTTAAATACAGCTCTTCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAgc                                                >  1:60363/1‑137 (MQ=255)
                                                                                      aCAAAAAGAACTCCATTCTTCATCAAGCTATGTGCCATTTTTAAATACAGCTCTTCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAgcaaat                                            >  6:114764/1‑134 (MQ=255)
                                                                                        aaaaAGAACTCCATTCTTCTTCAAGCTATGTGCCATTTTTAAATACAGCTCTTCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATtg                                          >  1:151721/1‑139 (MQ=255)
                                                                                        aaaaAGAACTCCATTCTTCTTCAAGCTATGTGCCATTTTTAAATACAGCTCTTCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATtg                                          >  2:442834/1‑139 (MQ=255)
                                                                                        aaaaAGAACTCCATTCTTCTTCAAGCTATGTGCCATTTTTAAATACAGCTCTTCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATtg                                          >  7:137227/1‑139 (MQ=255)
                                                                                        aaaaAGAACTCCATTCTTCTTCAAGCTATGTGCCATTTTTAAATACAGCTCTTCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATtg                                          >  3:111693/1‑139 (MQ=255)
                                                                                        aaaaAGAACTCCATTCTTCTTCAAGCTATGTGCCATTTTTAAATACAGCTCTTCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACGATTAAGTCAAAGTCTTGTTAATAACGATCCGCCAGCAAATtg                                          >  5:254012/1‑139 (MQ=255)
                                                                                        aaaaAGAACTCCAATCTTCTTCAAGCTATGTGCCATTTTTAAATACAGCTCTTACTTCGCGCTTTATGTAAAATAAATAAATACATAGAGGCACAAGATTAAGTAAAACTCTTGTTCAAAAAGATCCGCCAGCAAATtg                                          >  7:2940/1‑139 (MQ=255)
                                                                                                   cATTCTTCTTCAAGCTATGTGCCATTTTTAAATACAGCTCTTCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGca                                                                                        <  1:74885/82‑1 (MQ=255)
                                                                                                   cATTCTTCTTCAAGCTATGTGCCATTTTTAAATACAGCTCTTCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGca                                                                                        >  2:74885/1‑82 (MQ=255)
                                                                                                   cATTCTTCTTCAAGCTATGTGCCATTTTTAAATACAGCTCTTCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACTCGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGa                                <  8:123737/138‑1 (MQ=255)
                                                                                                   cATTCTTCTTCAAGCTATGTGCCATTTTTAAATACAGCTCTTCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGa                                <  8:201869/138‑1 (MQ=255)
                                                                                                           atCAAGCTATGTGCGATTTTTTAATACCGCGCTTCCTTGGCGCTTTCTGTTAAATAATTAATTTCATTGCGGCACAGGATTAAGGCTAAGTCTAGTTCTTAAAGATCCGCCAGAAAATTTTGATTATTGAATGtattat                       >  2:345789/2‑139 (MQ=255)
                                                                                                             cAAGCTATGTGCCATTTTTAAATACAGCTCTTCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTc                     >  8:254042/1‑139 (MQ=255)
                                                                                                                    tgtgCCATTTTTAAATACAGCTCTTCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGa              >  5:19195/1‑139 (MQ=255)
                                                                                                                    tgtgCCATTTTTAAATACAGCTCTTCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGa              >  3:71555/1‑139 (MQ=255)
                                                                                                                            ttttAAATACAGCTCTTCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGTCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCgtt      >  6:68061/1‑137 (MQ=255)
                                                                                                                            ttttAAATACAGCTCTTCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCgtt      >  8:306419/1‑137 (MQ=255)
                                                                                                                               taaatacaGCTCTTCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGtttt    <  7:306419/138‑1 (MQ=255)
                                                                                                                               taaatacaGCTCTTCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGtttt    <  8:2940/138‑1 (MQ=255)
                                                                                                                               taaatacaGCTCTTCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGtttt    <  1:442834/138‑1 (MQ=255)
                                                                                                                               taaatacaGCTCTTCCTTCGCGCCTTCTGTAAAATAAATAAATACATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGtttt    <  5:114764/138‑1 (MQ=255)
                                                                                                                               taaatacaGCTCTTACTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGGTTTTTGAATGTAATATTCTTTTTGATTTCCGtttt    <  1:252732/138‑1 (MQ=255)
                                                                                                                                 aatacaGCTCTTCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGttttca  <  4:71555/138‑3 (MQ=255)
                                                                                                                                     |                                                                                                                                     
AGTCAGTCTATCTTTTTTGATAAAAGAATGTATCGGCTGGAGCCAGGCCAAATTTCTCCGGATTGAAGATTTGCTCGGTGCTGCCGACAAATAGGACTCCATTCTTTTTCAAGCTATGTGCCATTTTTAAATAAAGCTCTTCCTTGGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACAATTAAGTCAAAGTCTTGTTCATAACGATCAGCCAGTAGATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGCTTTTA  >  CP006881/2198205‑2198471

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A