Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A14 F56 I20 R2 1958 92.1 3575798 91.1% 3257551 126.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 2,198,466 C→T A173T (GCG→ACG)  U712_11015 ← Chemotaxis protein methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,198,4660CT100.0% 151.6 / NA 49A173T (GCG→ACG) U712_11015Chemotaxis protein methyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (28/21);  total (28/21)

TAAATAAAGCTCTTCCTTGGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACAATTAAGTCAAAGTCTTGTTCATAACGATCAGCCAGTAGATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGCTTTTACTTCATAACTTCTATTTGCATTTTGAGTAAAATAACGATCCTTCACAGATGAAGGTACTTCCTGTAAAGACCTCTCCTGATAGACGCCTTTTTTCGCTTTCTCCAATGCTTTTTCATCAATATCAGTCGC  >  CP006881/2198332‑2198601
                                                                                                                                      |                                                                                                                                       
taaatacaGCTCTTCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGtttt                                                                                                                                      <  8:2940/138‑1 (MQ=255)
taaatacaGCTCTTCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGtttt                                                                                                                                      <  7:306419/138‑1 (MQ=255)
taaatacaGCTCTTCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGtttt                                                                                                                                      <  1:442834/138‑1 (MQ=255)
taaatacaGCTCTTCCTTCGCGCCTTCTGTAAAATAAATAAATACATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGtttt                                                                                                                                      <  5:114764/138‑1 (MQ=255)
taaatacaGCTCTTACTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGGTTTTTGAATGTAATATTCTTTTTGATTTCCGtttt                                                                                                                                      <  1:252732/138‑1 (MQ=255)
  aatacaGCTCTTCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGttttca                                                                                                                                    <  4:71555/138‑3 (MQ=255)
    tacaGCTCTTACTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACtt                                                                                                                                 <  2:81286/136‑1 (MQ=255)
       aGCTCTTCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCAt                                                                                                                              <  2:406119/139‑1 (MQ=255)
         ctctTCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATaa                                                                                                                            >  4:71972/1‑139 (MQ=255)
         ctctTCCTTCGCGCTTTCTGTAAAATAAATAAATAAATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTATTTAATGTAATATTCTTTTTGATATCCGTTTTCACTTCATa                                                                                                                             >  5:243376/1‑138 (MQ=255)
            ttCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAAc                                                                                                                           <  6:108278/137‑1 (MQ=255)
            ttCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACtt                                                                                                                         >  4:57383/1‑139 (MQ=255)
              ccTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACTTc                                                                                                                        >  5:223715/1‑138 (MQ=255)
                         tCTGTAAAATAAATAAATACATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGtttt                                                                                                            >  3:337826/1‑139 (MQ=255)
                                    aataaataCATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATaa                                                                                                 <  6:223715/139‑1 (MQ=255)
                                    aataaataCATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATaa                                                                                                 <  3:71972/139‑1 (MQ=255)
                                      taaataCATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACg                                                                                               <  7:346063/139‑1 (MQ=255)
                                        aataCATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGAt                                                                                             <  4:305137/139‑1 (MQ=255)
                                        aataCATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGAt                                                                                             <  8:137227/139‑1 (MQ=255)
                                            cATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGCTCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGATCttt                                                                                         >  8:103588/1‑139 (MQ=255)
                                            cATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGATCttt                                                                                         <  2:60363/139‑1 (MQ=255)
                                               tGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGATCTTTca                                                                                       >  8:169309/1‑138 (MQ=255)
                                               tGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGATCTTTca                                                                                       >  5:71533/1‑138 (MQ=255)
                                               tGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGATCTTTca                                                                                       >  3:60538/1‑138 (MQ=255)
                                               tGCGGCACACGATTAAGTCAAAGGCTTGTTCATAACGATCCGCCAGCAAATTGTGATTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGATCTTTca                                                                                       >  4:72123/1‑138 (MQ=255)
                                                 cGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGATCTTTca                                                                                       >  3:98203/1‑136 (MQ=255)
                                                  ggCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGATCTTTCACAGa                                                                                   <  4:337826/139‑1 (MQ=255)
                                                    cacacgATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATAGTGCTTTTTCAATGTATTATTCTTTTTGATTTCCGTTTTCACTTCATACCTACTATTTGCGTTTTGCG‑AAAATAACGATCTTTCACAGAtag                                                                                >  3:276149/1‑137 (MQ=255)
                                                      cacgATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGATCTTTCACAGAtagc                                                                               >  6:25971/1‑136 (MQ=255)
                                                                   aaGTCTTGTTCATAACGATCCGCCAGCAATTTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTATTGCGTAAAATAACGATCTTTCACAGATAGCGGCACTTCCTga                                                                   >  1:365270/1‑137 (MQ=255)
                                                                   aaGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGCTCTTTCACAGATAGCGGCCCTTCCTGt                                                                   >  5:282011/1‑138 (MQ=255)
                                                                         tGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGAcc                                                            >  3:65381/1‑139 (MQ=255)
                                                                         tGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGAcc                                                            >  8:4063/1‑139 (MQ=255)
                                                                          gTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGATCTTTCACAGAtagcgg                                                                             >  4:98381/1‑116 (MQ=255)
                                                                          gTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGATCTTTCACAGAtagcgg                                                                             <  3:98381/121‑6 (MQ=255)
                                                                           ttCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCAATTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGAccg                                                           <  7:405160/138‑2 (MQ=255)
                                                                               tAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCt                                                      >  1:321569/1‑139 (MQ=255)
                                                                                aaCGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCTTAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACAGCTCCt                                                      >  8:372215/1‑138 (MQ=255)
                                                                                aaCGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCt                                                      >  4:296291/1‑138 (MQ=255)
                                                                                     tccgCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGATCTTTCACAGAta                                                                                 >  5:134718/4‑105 (MQ=255)
                                                                                     tccgCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGATCTTTCACAGAta                                                                                 <  6:134718/103‑2 (MQ=255)
                                                                                     tccgCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGATCTTTCACAGATAGCGGCTCTTCCTGTAAAGACCGCTCCTGATaaa                                                >  2:168428/4‑137 (MQ=255)
                                                                                     tccgCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATaaa                                                >  5:89194/4‑137 (MQ=255)
                                                                                                    tgCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCt                                  <  2:133478/138‑1 (MQ=255)
                                                                                                              aTGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCt                                  <  7:217974/128‑1 (MQ=255)
                                                                                                              aTGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCt                                  >  8:217974/1‑128 (MQ=255)
                                                                                                                   atatTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCttttt                  <  6:254012/139‑1 (MQ=255)
                                                                                                                   atatTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCttttt                  <  2:151721/139‑1 (MQ=255)
                                                                                                                   atatTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCtttt                   <  4:376529/138‑1 (MQ=255)
                                                                                                                      ttCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTtcat               <  6:89194/139‑1 (MQ=255)
                                                                                                                           tttGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGATCTTTCACAGa                                                                                   <  3:39126/66‑1 (MQ=255)
                                                                                                                           tttGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGATCTTTCACAGa                                                                                   >  2:212086/1‑66 (MQ=255)
                                                                                                                           tttGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGATCTTTCACAGa                                                                                   >  4:39126/1‑66 (MQ=255)
                                                                                                                           tttGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGATCTTTCACAGa                                                                                   <  1:212086/66‑1 (MQ=255)
                                                                                                                                   ccGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGc  >  4:331592/1‑139 (MQ=255)
                                                                                                                                      |                                                                                                                                       
TAAATAAAGCTCTTCCTTGGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACAATTAAGTCAAAGTCTTGTTCATAACGATCAGCCAGTAGATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGCTTTTACTTCATAACTTCTATTTGCATTTTGAGTAAAATAACGATCCTTCACAGATGAAGGTACTTCCTGTAAAGACCTCTCCTGATAGACGCCTTTTTTCGCTTTCTCCAATGCTTTTTCATCAATATCAGTCGC  >  CP006881/2198332‑2198601

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A