Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A14 F56 I0 R2 216 87.9 3565874 95.6% 3408975 127.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 3,724,274 C→T 100% E185K (GAA→AAA)  U712_19200 ← SacPA operon antiterminator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068813,724,2740CT100.0% 411.3 / NA 116E185K (GAA→AAA) U712_19200SacPA operon antiterminator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (43/73);  total (43/73)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 116 total aligned reads displayed.

AATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGACTGACTGCGTATCTCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTCTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGAAGAGACACGCCCAAT  >  CP006881/3724142‑3724407
                                                                                                                                    |                                                                                                                                     
aaTAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTAtttt                                                                                                                                 <  6:233678/139‑1 (MQ=255)
        aTCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCa                                                                                                                         <  8:138060/139‑1 (MQ=255)
          ctcctcGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGCCCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATGAtt                                                                                                                       >  5:239279/1‑139 (MQ=255)
          ctcctcGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTAtttt                                                                                                                                 <  1:320741/129‑1 (MQ=255)
          ctcctcGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTAtttt                                                                                                                                 >  2:320741/1‑129 (MQ=255)
          ctcctcGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCAtt                                                                                                                       >  4:153616/1‑139 (MQ=255)
          ctcctcGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCAtt                                                                                                                       >  5:258313/1‑139 (MQ=255)
          ctcctcGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCAtt                                                                                                                       >  7:178868/1‑139 (MQ=255)
          ctcctcGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCAtt                                                                                                                       >  7:268483/1‑139 (MQ=255)
             ctcGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCCTCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCt                                                                                                                    <  6:55234/139‑1 (MQ=255)
             ctcGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCt                                                                                                                    <  4:127275/139‑1 (MQ=255)
             ctcGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCt                                                                                                                    <  6:349727/139‑1 (MQ=255)
               cGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTAt                                                                                                                           <  4:113099/130‑1 (MQ=255)
               cGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTAt                                                                                                                           >  3:113099/1‑130 (MQ=255)
                 tCCATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGa                                                                                                                >  1:107041/1‑139 (MQ=255)
                   cATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGAt                                                                                                               <  1:196944/138‑1 (MQ=255)
                   cATACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTATCTTTCATTTGTTTGAt                                                                                                               >  2:433747/1‑138 (MQ=255)
                    aTACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCa                                                                                                             <  7:179093/139‑1 (MQ=255)
                    aTACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCa                                                                                                             <  1:433747/139‑1 (MQ=255)
                    aTACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCa                                                                                                             <  6:258313/139‑1 (MQ=255)
                    aTACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCa                                                                                                             <  1:233801/139‑1 (MQ=255)
                    aTACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCa                                                                                                             <  4:245409/139‑1 (MQ=255)
                    aTACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGAGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCa                                                                                                             <  3:103206/139‑1 (MQ=255)
                     tACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCTCGTATCGCAAATGCGTGACAAGGCTTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTAAATAATTTCTTTGATCAt                                                                                                            >  8:186855/1‑139 (MQ=255)
                     tACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCt                                                                                                                    <  2:133533/131‑1 (MQ=255)
                     tACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCt                                                                                                                    >  1:133533/1‑131 (MQ=255)
                     tACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCAt                                                                                                            >  2:180321/1‑139 (MQ=255)
                     tACGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGAGAAAAGTATTGTTTTATTTTATCTATCATTTCTTTGATCAt                                                                                                            >  2:393203/1‑139 (MQ=255)
                       cGATGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATgg                                                                                                          <  8:362501/139‑1 (MQ=255)
                          tGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTGTATCATTTCTTTGATCATGGTCg                                                                                                       >  5:331709/1‑139 (MQ=255)
                          tGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATgg                                                                                                          <  1:144653/136‑1 (MQ=255)
                          tGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCg                                                                                                       >  8:45836/1‑139 (MQ=255)
                          tGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCg                                                                                                       >  7:371876/1‑139 (MQ=255)
                          tGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCg                                                                                                       >  7:275723/1‑139 (MQ=255)
                          tGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTACTTTGATCATgg                                                                                                          >  2:144653/1‑136 (MQ=255)
                          tGGAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGGTTTATTTTCTCTATCATTTCTTTGATCATGGTCg                                                                                                       >  6:243618/1‑139 (MQ=255)
                            gAGCGCTTCATTTGATTCCAACCGGCTGACCTCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTAGATTAAATTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGTTCGTa                                                                                                     >  2:88517/1‑139 (MQ=255)
                            gAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTa                                                                                                     <  4:58984/139‑1 (MQ=255)
                            gAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTa                                                                                                     <  1:299468/139‑1 (MQ=255)
                            gAGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATAATTTCTTTGATCATGGTCGTa                                                                                                     >  2:308064/1‑139 (MQ=255)
                             aGCGCTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGTCGTTGATAGGAAATGCTGTTTTCATCCACCTTTAGATTAAAGTATTGTTTTTTTTTCTCTATCATTTCTTTGATCATGGTCGTa                                                                                                     <  8:138924/138‑1 (MQ=255)
                               cgcTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGc                                                                                                  >  3:75844/1‑139 (MQ=255)
                                 cTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTCTGCtt                                                                                                >  8:146558/1‑139 (MQ=255)
                                 cTTCATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCtt                                                                                                <  6:291862/139‑1 (MQ=255)
                                  ttCATTTGACTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCtt                                                                                                >  4:285255/1‑138 (MQ=255)
                                    cATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCa                                                                                              <  7:83151/138‑1 (MQ=255)
                                    cATTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCa                                                                                              <  3:153616/138‑1 (MQ=255)
                                     aTTTGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAg                                                                                             <  5:379705/138‑1 (MQ=255)
                                      tttGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAgcg                                                                                           <  2:107041/139‑1 (MQ=255)
                                      tttGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAgcg                                                                                           <  5:150296/139‑1 (MQ=255)
                                      tttGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAgcg                                                                                           <  3:391743/139‑1 (MQ=255)
                                      tttGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAgcg                                                                                           <  7:37715/139‑1 (MQ=255)
                                      tttGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAgcg                                                                                           <  1:282752/139‑1 (MQ=255)
                                      tttGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAgcg                                                                                           <  4:75844/139‑1 (MQ=255)
                                        tGATTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATTCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTTATCATGGTCGTATGCTTCAgcgc                                                                                          <  3:252565/138‑1 (MQ=255)
                                          aTTCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTg                                                                                        <  1:393203/138‑1 (MQ=255)
                                            tCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCa                                                                                                                         >  3:373424/1‑103 (MQ=255)
                                            tCCAACCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCa                                                                                                                         <  4:373424/103‑1 (MQ=255)
                                                aCCGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCTTATGCTTCAGAGCTGAATACAt                                                                                 >  7:29105/1‑139 (MQ=255)
                                                 ccGGCTGACCGCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATg                                                                                <  3:110437/139‑1 (MQ=255)
                                                   ggctgaccgCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGct                                                                              <  5:168975/137‑1 (MQ=255)
                                                   ggctgaccgCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGct                                                                              <  2:10949/137‑1 (MQ=255)
                                                    gctgaccgCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCa                                                                                                             <  3:294865/106‑1 (MQ=255)
                                                    gctgaccgCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCa                                                                                                             >  4:294865/2‑107 (MQ=255)
                                                          cgCGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGCTCTCCGca                                                                       <  7:186855/138‑1 (MQ=255)
                                                            cGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGCTCTCCGcatc                                                                     <  5:251001/139‑1 (MQ=255)
                                                            cGTATCGCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTGTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATCCATGCTCTCCGcatc                                                                     >  4:30581/1‑139 (MQ=255)
                                                                  gcAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGCTCTCCGCATCCATCtt                                                               <  5:416022/138‑1 (MQ=255)
                                                                  gcAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGCTCTCCGCATCCATCt                                                                <  1:341130/137‑1 (MQ=255)
                                                                   cAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGCTCTCCGCATCCATCt                                                                >  5:255565/1‑137 (MQ=255)
                                                                    aaaTGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCGACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGCTCTCCGCATCCATCTTCg                                                             <  5:244158/139‑1 (MQ=255)
                                                                    aaaTGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGCTCTCCGCATCCATCTTCg                                                             <  7:45836/139‑1 (MQ=255)
                                                                    aaaTGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGCTCTCCGCATCCATCTTCg                                                             <  2:122191/139‑1 (MQ=255)
                                                                         cGTGTCAAGGCGGTGATAGGAATTGCTGTTTTCATGCACCTTTTGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGGCGTATGCTTCAGCGCTGAATACTTGCTGTCCGCATCCATCTTCCGCGTa                                                        <  6:321400/139‑1 (MQ=255)
                                                                           tGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGCTCTCCGCATCCATCTTCGCCGTATg                                                      >  3:398938/1‑139 (MQ=255)
                                                                           tGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGCTCTCCGCATCCATCTTCGCCGTATg                                                      >  8:283156/1‑139 (MQ=255)
                                                                             aCAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGCTCTCCGCATCCATCTTCGCCGTATgg                                                     <  5:243618/138‑1 (MQ=255)
                                                                              cAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGCTCTCCGCATCCATCTTCGCCGTATGGAt                                                   <  4:395596/139‑1 (MQ=255)
                                                                                 ggCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAgcg                                                                                           <  4:9387/96‑1 (MQ=255)
                                                                                 ggCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAgcg                                                                                           >  3:9387/1‑96 (MQ=255)
                                                                                 ggCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGCTCTCCGCATCCATCTTCGCCGTATGGAtgtg                                                <  1:329708/139‑1 (MQ=255)
                                                                                  gCGTTGATAGGAAATGCTGTTTTCATCCCCCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGCTCTCCGCATCCATCTTCGCCGGATGGAtgtg                                                >  2:345905/1‑138 (MQ=255)
                                                                                  gCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTAAGCGCTGCATACATGCTCTCCGCATCCATCTTCCCCGTATTGAtgtg                                                >  4:244175/1‑138 (MQ=255)
                                                                                   cGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGCTCTCCGCATACCTAGTAGCCGTATGGAtgtg                                                >  2:329708/1‑137 (MQ=255)
                                                                                    gTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAg                                             <  7:185175/139‑1 (MQ=255)
                                                                                       gagaGGAAATGGTGGTGTCAGCCTCCTTTCGATTAAAGTATTGTTTTATTTTGTCTTTCATTTCTTAGATCATGGTCGTATGATGCAGCGCTGACTACATGCTCTCCGGATCCATCTTCGCCGTATGGATGTGAAggg                                           <  8:341034/135‑1 (MQ=255)
                                                                                       gATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAggg                                           <  6:312488/138‑1 (MQ=255)
                                                                                       gATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAggg                                           <  4:360307/138‑1 (MQ=255)
                                                                                       gATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAggg                                           <  4:398938/138‑1 (MQ=255)
                                                                                       gATAGGAAATGCTGTTTTCATCCACATTTGGATTATAGTATTGTTTTTTTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAggg                                           <  2:303548/138‑1 (MQ=255)
                                                                                                       ttCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAggg                                           <  6:324822/122‑1 (MQ=255)
                                                                                                       ttCATCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAggg                                           >  5:324822/1‑122 (MQ=255)
                                                                                                          aTCCACCTTTCGATTAAAGTATTGTTTTTTTTTCTCTTTCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATTCATGCTCTCCGCATCCCTCTTCGCCGTATGGATGTGAAGGGGAATATTACCCGCTTCATCt                       >  2:438366/1‑139 (MQ=255)
                                                                                                           tCCACCTTTCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCtt                      <  8:371876/139‑1 (MQ=255)
                                                                                                               acTTTCGATTAAAGTATTGTTTTATTTTCTATATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTTAATACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTTAAGGGCAATATAACCCGCTTCATCTTCAgg                  <  1:88517/138‑1 (MQ=255)
                                                                                                                  ttCGATTAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAgg                  >  8:373925/1‑136 (MQ=255)
                                                                                                                       ttAAAGTATTGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGac           <  4:311041/138‑1 (MQ=255)
                                                                                                                               ttGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAAt  <  7:373925/139‑1 (MQ=255)
                                                                                                                               ttGTTTTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAAt  <  7:324237/139‑1 (MQ=255)
                                                                                                                               ttGTTTTATTTTCTCTATCATTTCTTTGATCATGGGCGTATGCTTCAGCGCTGAATACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAAt  <  8:268483/139‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                     
AATAAAGCATCTCCTCGTCCATACGATGGAGCGCTTCATTTGATTCCAACCGACTGACTGCGTATCTCAAATGCGTGACAAGGCGTTGATAGGAAATGCTGTTTTCATCCACCTTTCGATTAAAGTATTGTTCTATTTTCTCTATCATTTCTTTGATCATGGTCGTATGCTTCAGCGCTGAATACATGCTCTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGAAGAGACACGCCCAAT  >  CP006881/3724142‑3724407

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A