Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A28 F56 I1 R1 120 79.0 2973134 95.3% 2833396 126.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 3,723,438 T→C T84T (ACT→ACC)  U712_19195 → putative transporter ywcJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068813,723,4380TC98.4% 239.4 / NA 64T84T (ACT→ACC) U712_19195putative transporter ywcJ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (18/45);  minor base G (1/0);  total (19/45)
Fisher's exact test for biased strand distribution p-value = 2.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATTCGGCATTACAGCCGCAAGCAAAACAGGCAGTTATTTCTCTATGGCGGATTCTCCGTTTGCTTTTCCGGCAGCCGCTGTCACATTCGGGGCCGCTATTCTGCTGATTGCTTACGGAGGCGGAGATTTA‑TTTACTGGCAACACTTTTTATTTCACTTATACCGCGCTCCGAAAAAAAATTCGCTGGCGAGACACCCTGTACCTGTGGATGTCAAGCTATGCCGGCAATTTGATCGGCGCCATTCTATTTGCCATTCTGATCAGCGCGAC  >  CP006881/3723303‑3723572
                                                                                                                                        |                                                                                                                                      
ttttggCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTA‑TTTACCGGc                                                                                                                                     >  2:27554/5‑139 (MQ=255)
 tttggCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCGGATGATTGCTTACGGAGGCGGAGATTTA‑TTTACCGGCa                                                                                                                                    >  3:263565/4‑139 (MQ=255)
  ttggCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTAGGGGCCGCTATTCTGATGATTGCATACGGAGGCGGAGATTTA‑TTTACCGGCaa                                                                                                                                   >  6:244237/3‑139 (MQ=255)
  ttcgCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTA‑TTTACCGGCaa                                                                                                                                   >  2:9041/4‑139 (MQ=255)
   tggCACTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTA‑TTTACCGGCaa                                                                                                                                   <  1:166330/137‑1 (MQ=255)
    ggCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTA‑TTTACCGGCAac                                                                                                                                  <  4:73215/138‑1 (MQ=255)
       aTTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTA‑TTTACCGGCAacacct                                                                                                                              <  2:197395/139‑3 (MQ=255)
       aTTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTA‑TTTACCGGCAacacct                                                                                                                              <  7:220083/139‑3 (MQ=255)
            aGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTA‑TTTACCGGCAACACCTTTTAt                                                                                                                         <  6:302774/139‑1 (MQ=255)
            aGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTA‑TTTACCGGCAACACCTTTTAt                                                                                                                         <  1:141214/139‑1 (MQ=255)
            aGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTA‑TTTACCGGCAACACCTTTTAt                                                                                                                         <  3:251705/139‑1 (MQ=255)
            aGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTA‑TTTACCGGCAACACCTTTTAt                                                                                                                         <  4:150784/139‑1 (MQ=255)
            aGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTA‑TTTACCGGCAACACCTTTTAt                                                                                                                         <  7:325329/139‑1 (MQ=255)
            aGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTA‑TTTACCGGCAACACCTTTTAt                                                                                                                         <  5:7500/139‑1 (MQ=255)
            aGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTA‑TTTACCGGCAACACCTTTTAt                                                                                                                         <  7:161162/139‑1 (MQ=255)
              ccGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTA‑TTTACCGGCAACACCTTTTAttt                                                                                                                       <  5:287708/139‑1 (MQ=255)
              ccGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTA‑TTTACCGGCAACACCTTTTAttt                                                                                                                       <  5:244237/139‑1 (MQ=255)
              ccGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTA‑TTTACCGGCAACACCTTTTAttt                                                                                                                       <  6:12450/139‑1 (MQ=255)
              ccGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCGATTCTGAGTTATGATTACGGAGGCGGTGAATTATTTTACGGGC‑ACACCtttttttt                                                                                                                       >  6:229118/1‑139 (MQ=255)
                gcaagcaaAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTA‑TTTACCGGCAACACCTTTTATTTCa                                                                                                                     >  2:33282/1‑139 (MQ=255)
                 caagcaaAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGGCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTA‑TTTACCGGCAACACCTTTTATTTCAc                                                                                                                    <  1:206831/139‑1 (MQ=255)
                         aCAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTA‑TTTACCGGCAACACCTTTTATTTCACCTATACcg                                                                                                            <  7:75612/139‑1 (MQ=255)
                                    tttCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTA‑TTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGgaa                                                                                                  <  4:320298/138‑1 (MQ=255)
                                    tttCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTA‑TTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGgaa                                                                                                  <  5:58819/138‑1 (MQ=255)
                                                  aTTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTA‑TTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCg                                                                                   >  5:21287/1‑139 (MQ=255)
                                                  aTTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCGTATGATTGCTTACGGAGGCCGAGATTTA‑TTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCATCTGGCg                                                                                   >  8:128513/1‑139 (MQ=255)
                                                     ctcCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTA‑TTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCgcga                                                                                <  7:253893/139‑1 (MQ=255)
                                                     ctcCGTTTGCCTTTCCGGCAGCCGCGGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTA‑TTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCgcga                                                                                <  3:207375/139‑1 (MQ=255)
                                                        cGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTA‑TTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCgcga                                                                                <  2:696/136‑1 (MQ=255)
                                                        cGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTA‑TTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCgcga                                                                                <  5:66239/136‑1 (MQ=255)
                                                        cGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTA‑TTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCgcga                                                                                <  3:127941/136‑1 (MQ=255)
                                                        cGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTA‑TTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCgcga                                                                                <  1:33282/136‑1 (MQ=255)
                                                        cGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTA‑TTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCgcga                                                                                <  1:29444/136‑1 (MQ=255)
                                                        cGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTA‑TTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCgcga                                                                                <  1:27554/136‑1 (MQ=255)
                                                        cGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTA‑TTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCgcga                                                                                <  3:93149/136‑1 (MQ=255)
                                                        cGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTA‑TTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGaca                                                                              <  6:273297/138‑1 (MQ=255)
                                                        cGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTA‑TTTACCGGCAACAC‑CTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGaca                                                                              <  3:79005/137‑1 (MQ=255)
                                                        cGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGACGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTA‑TTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCgcga                                                                                <  1:9041/136‑1 (MQ=255)
                                                        cGTTTGCATTTCCGGCAGCCGCTGTCACTTTCGGGGCAGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTA‑TTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCgcga                                                                                <  8:119434/136‑1 (MQ=255)
                                                         gTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTA‑TTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACAcc                                                                            <  4:83837/139‑1 (MQ=255)
                                                            tGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTA‑TTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCta                                                                         >  7:244403/1‑138 (MQ=255)
                                                             gcctttCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTA‑TTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACAccata                                                                         <  3:127431/135‑4 (MQ=255)
                                                             gcctttCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTA‑TTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCta                                                                         <  4:336772/135‑2 (MQ=255)
                                                                                                       atgATTGCTTACGGAGGCGGAGATTTA‑TTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAAt                                      <  3:287134/130‑1 (MQ=255)
                                                                                                       atgATTGCTTACGGAGGCGGAGATTTA‑TTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAAt                                      >  4:287134/2‑131 (MQ=255)
                                                                                                       atgATTGCTTACGGAGGCGGAGATTTA‑TTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCa                              <  1:118304/138‑1 (MQ=255)
                                                                                                       atgATTGCTTACGGAGGCGGAGATTTA‑TTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCa                              >  7:174210/2‑139 (MQ=255)
                                                                                                          aTTGCTTACGGAGGCGGAGATTTA‑TTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTc                           >  4:292769/1‑139 (MQ=255)
                                                                                                           ttGCTTACGGAGGCGGAGATTTA‑TTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCt                          <  6:21287/139‑1 (MQ=255)
                                                                                                            tGCTTACGGAGGCGGAGATTTA‑TTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTgg                                                                                     >  5:326509/1‑79 (MQ=255)
                                                                                                            tGCTTACGGAGGCGGAGATTTA‑TTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTgg                                                                                     <  6:326509/79‑1 (MQ=255)
                                                                                                            tGCTTACGGAGGCGGAGATTTA‑TTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTtgt                                                                  >  3:137531/1‑98 (MQ=255)
                                                                                                            tGCTTACGGAGGCGGAGATTTA‑TTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTtgt                                                                  <  4:137531/98‑1 (MQ=255)
                                                                                                            tGCTTACGGAGGCGGAGATTTA‑TTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCtg                         >  4:73781/1‑138 (MQ=255)
                                                                                                              cTTACGGAGGCGGAGATTTA‑TTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGtt                       >  2:127352/1‑139 (MQ=255)
                                                                                                                 aCGGAGGCGGAGATTTA‑TTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTg                     <  1:2620/138‑1 (MQ=255)
                                                                                                                 aCGGAGGCGGAGATTTA‑TTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTg                     <  3:292769/138‑1 (MQ=255)
                                                                                                                 aCGGAGGCGGAGATTTA‑TTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCGGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGtttt                     <  2:150190/138‑2 (MQ=255)
                                                                                                                     aGGCGGAGATTTA‑TTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCAtc                <  8:174210/139‑2 (MQ=255)
                                                                                                                       gCGGAGATTTA‑TTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACACTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCt              >  8:20727/1‑139 (MQ=255)
                                                                                                                        cGGAGATTTA‑TTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGc                    <  1:214314/132‑1 (MQ=255)
                                                                                                                        cGGAGATTTA‑TTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGc                    >  2:214314/1‑132 (MQ=255)
                                                                                                                        cGGAGATTTA‑TTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCt              >  6:366425/1‑138 (MQ=255)
                                                                                                                                     taCCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGAc  <  8:244403/138‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                      
ATTCGGCATTACAGCCGCAAGCAAAACAGGCAGTTATTTCTCTATGGCGGATTCTCCGTTTGCTTTTCCGGCAGCCGCTGTCACATTCGGGGCCGCTATTCTGCTGATTGCTTACGGAGGCGGAGATTTA‑TTTACTGGCAACACTTTTTATTTCACTTATACCGCGCTCCGAAAAAAAATTCGCTGGCGAGACACCCTGTACCTGTGGATGTCAAGCTATGCCGGCAATTTGATCGGCGCCATTCTATTTGCCATTCTGATCAGCGCGAC  >  CP006881/3723303‑3723572

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A