Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A28 F56 I1 R1 120 79.0 2973134 95.3% 2833396 126.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 3,723,915 C→T V243V (GTC→GTT)  U712_19195 → putative transporter ywcJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068813,723,9150CT100.0% 216.9 / NA 66V243V (GTC→GTT) U712_19195putative transporter ywcJ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (35/31);  total (35/31)

CGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCACTTTTGATCGAGCACCCTGATACAGTGACAGTAATGGGAGCAGTCAGAAACTTAATTCCCGTTACACTCGGCAACCTGACCGCGGGAATGGTCATGATGGGCTGGATGTATTACACGCTCAATCCCGATAAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGC  >  CP006881/3723783‑3724032
                                                                                                                                    |                                                                                                                      
tgAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTatga                                                                                                                    <  1:163108/136‑1 (MQ=255)
tgAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTatga                                                                                                                    <  1:167967/136‑1 (MQ=255)
  aaCACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATgg                                                                                                                 <  6:147397/138‑1 (MQ=255)
       aGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGa                                                                                                           >  8:142660/1‑139 (MQ=255)
        gCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGAt                                                                                                          <  4:210533/139‑1 (MQ=255)
            tGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTac                                                                                                      >  2:323710/1‑138 (MQ=255)
            tGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTac                                                                                                      >  2:17603/1‑138 (MQ=255)
             gCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTac                                                                                                      >  6:245085/1‑137 (MQ=255)
               cAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGtactac                                                                                                   <  8:21450/139‑1 (MQ=255)
                  tatGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTacaca                                                                                                <  1:323710/139‑2 (MQ=255)
                     gtgCACATTCGCCCTCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACt                                                                                              >  5:142497/1‑138 (MQ=255)
                      tgCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACtg                                                                                             >  4:367440/1‑137 (MQ=255)
                        cacaTTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATggg                                                                                                                <  8:10928/117‑1 (MQ=255)
                        cacaTTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATggg                                                                                                                >  7:10928/1‑117 (MQ=255)
                        cacaTTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAAt                                                                                          >  5:80418/1‑139 (MQ=255)
                        cacaTTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAAt                                                                                          >  1:325520/1‑139 (MQ=255)
                             tCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGa                                                                                     >  3:6478/1‑139 (MQ=255)
                               gCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGc                                                                                                               <  2:180052/111‑1 (MQ=255)
                               gCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGc                                                                                                               >  1:180052/1‑111 (MQ=255)
                                       gcTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATaaaa                                                                            >  1:181162/2‑138 (MQ=255)
                                           ttGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACt                                                                      >  5:280129/1‑139 (MQ=255)
                                               tCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCa                                                                  <  6:138446/139‑1 (MQ=255)
                                                ccaGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCGCGGCAATCTGACCGCGGTAATTGTTATGATGGGCTGGATGTACTACACACTGAATCATGATCAATAAAAAA‑ACTTCCa                                                                  <  7:142660/136‑1 (MQ=255)
                                                 gAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATggg                                                                                                                >  4:352912/1‑92 (MQ=255)
                                                 gAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATggg                                                                                                                <  1:158357/92‑1 (MQ=255)
                                                 gAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATggg                                                                                                                <  3:352912/92‑1 (MQ=255)
                                                 gAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATggg                                                                                                                >  2:158357/1‑92 (MQ=255)
                                                         tGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATc                                                                                         >  4:102926/1‑107 (MQ=255)
                                                         tGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATc                                                                                         <  3:102926/107‑1 (MQ=255)
                                                                 tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTatg                                                                                                                     >  2:197045/1‑71 (MQ=255)
                                                                 tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTatg                                                                                                                     <  1:197045/71‑1 (MQ=255)
                                                                 tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAg                                                >  7:354718/1‑139 (MQ=255)
                                                                 tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAg                                                >  7:231051/1‑139 (MQ=255)
                                                                 tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAg                                                >  5:289230/1‑139 (MQ=255)
                                                                 tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAg                                                >  7:83513/1‑139 (MQ=255)
                                                                 tGACACTGATGGGAGCAGTCAGAAACTTAATCCCAGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAg                                                >  7:283482/1‑139 (MQ=255)
                                                                   acactgaTGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGt                                               >  5:22951/7‑138 (MQ=255)
                                                                     actgaTGGGCGCAGTCACAAACTTAATCCCCTTTACGCTCGGCAATCTGACCGCGTGAAAAGTTATGATGGTCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCaa                                            <  6:289230/135‑1 (MQ=255)
                                                                         aTGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAC‑ACTTCCAGAGATCACTCCCTg                                                    <  6:117691/127‑1 (MQ=255)
                                                                         aTGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTg                                                    >  5:117691/1‑127 (MQ=255)
                                                                         aTGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCaa                                            <  5:225644/135‑1 (MQ=255)
                                                                         aTGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCaa                                            >  6:225644/1‑135 (MQ=255)
                                                                           gggAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGt                                      >  4:298126/1‑139 (MQ=255)
                                                                           gggAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGt                                      <  2:181162/139‑1 (MQ=255)
                                                                           gggAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACAGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTCATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGt                                      >  3:366116/1‑139 (MQ=255)
                                                                              aGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGAt                                                             <  6:368786/113‑1 (MQ=255)
                                                                              aGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGAt                                                             >  5:368786/1‑113 (MQ=255)
                                                                              aGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTAt                                   >  4:321984/1‑139 (MQ=255)
                                                                                 aGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTcc                                >  5:205679/1‑139 (MQ=255)
                                                                                   tCAGATCCTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTAGCCCCGGCATCCTGATCAATAAAAAA‑ACTTCCAGCGCTCACTCCCTGCAAGTCGAAGCTGTTATTcc                                >  7:18478/1‑137 (MQ=255)
                                                                                   tCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTa                                                                                                       <  4:352506/67‑1 (MQ=255)
                                                                                   tCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGaa                                                                                           <  1:48303/79‑1 (MQ=255)
                                                                                       aaaCTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCga                              >  4:200430/1‑135 (MQ=255)
                                                                                             aaTCCCCGTTACGGGCGGCCATCAGACCGCGGGAATAGTTATGATGGGCGGGATGTACTACACACTGCATCCTGATAAATAAAAAACACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCtt                      <  2:226324/138‑1 (MQ=255)
                                                                                             aaTCCCCGTTACGCTCTGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCAGCAAGTCAAAGCGGTTATTCCGAGAGATCttc                     >  3:129355/1‑137 (MQ=255)
                                                                                              atccccGTTACGCTCGGCAATCTTACCGCGGGATTATTTATGATGGGCTGGTTGTACTACACCCTGAATCCTCATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGaa                   <  2:325520/136‑1 (MQ=255)
                                                                                              atccccGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGaa                   <  7:63782/136‑1 (MQ=255)
                                                                                              atccccGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGa                    <  8:354718/135‑1 (MQ=255)
                                                                                                     ttACGCTCGGCAATCTCACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGAGAAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTg            <  3:312378/139‑1 (MQ=255)
                                                                                                        cgcTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGAc         <  8:283482/137‑1 (MQ=255)
                                                                                                             ggCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCa    <  8:231051/139‑1 (MQ=255)
                                                                                                               cAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAAAAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGc  <  1:17603/139‑1 (MQ=255)
                                                                                                                  tcTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACa        >  7:280283/2‑130 (MQ=255)
                                                                                                                  tcTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACa        <  8:280283/129‑1 (MQ=255)
                                                                                                                          cgGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGAc                <  6:289045/114‑1 (MQ=255)
                                                                                                                          cgGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGAc                >  5:289045/1‑114 (MQ=255)
                                                                                                                                    |                                                                                                                      
CGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCACTTTTGATCGAGCACCCTGATACAGTGACAGTAATGGGAGCAGTCAGAAACTTAATTCCCGTTACACTCGGCAACCTGACCGCGGGAATGGTCATGATGGGCTGGATGTATTACACGCTCAATCCCGATAAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGC  >  CP006881/3723783‑3724032

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A