Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A28 F56 I1 R1 120 79.0 2973134 95.3% 2833396 126.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 3,723,952 A→C K256Q (AAA→CAA)  U712_19195 → putative transporter ywcJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068813,723,9520AC97.9% 349.2 / NA 97K256Q (AAA→CAA) U712_19195putative transporter ywcJ
Reads supporting (aligned to +/- strand):  ref base A (0/2);  new base C (66/29);  total (66/31)
Fisher's exact test for biased strand distribution p-value = 9.99e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACTTTTGATCGAGCACCCTGATACAGTGACAGTAATGGGAGCAGTCAGAAACTTAATTCCCGTTACACTCGGCAACCTGACCGCGGGAATGGTCATGATGGGCTGGATGTATTACACGCTCAATCCCGATAAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGACTCCGGCAAATGTAATTGATATTCACTTTTCAAA  >  CP006881/3723822‑3724086
                                                                                                                                  |                                                                                                                                       
gcTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATaaaa                                                                                                                                  >  1:181162/2‑138 (MQ=255)
    ttGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACt                                                                                                                            >  5:280129/1‑139 (MQ=255)
        tCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCa                                                                                                                        <  6:138446/139‑1 (MQ=255)
         ccaGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCGCGGCAATCTGACCGCGGTAATTGTTATGATGGGCTGGATGTACTACACACTGAATCATGATCAATAAAAAA‑ACTTCCa                                                                                                                        <  7:142660/136‑1 (MQ=255)
                          tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAg                                                                                                      >  7:231051/1‑139 (MQ=255)
                          tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAg                                                                                                      >  5:289230/1‑139 (MQ=255)
                          tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAg                                                                                                      >  7:83513/1‑139 (MQ=255)
                          tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAg                                                                                                      >  7:354718/1‑139 (MQ=255)
                          tGACACTGATGGGAGCAGTCAGAAACTTAATCCCAGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAg                                                                                                      >  7:283482/1‑139 (MQ=255)
                            acactgaTGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGt                                                                                                     >  5:22951/7‑138 (MQ=255)
                              actgaTGGGCGCAGTCACAAACTTAATCCCCTTTACGCTCGGCAATCTGACCGCGTGAAAAGTTATGATGGTCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCaa                                                                                                  <  6:289230/135‑1 (MQ=255)
                                  aTGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAC‑ACTTCCAGAGATCACTCCCTg                                                                                                          <  6:117691/127‑1 (MQ=255)
                                  aTGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTg                                                                                                          >  5:117691/1‑127 (MQ=255)
                                  aTGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCaa                                                                                                  >  6:225644/1‑135 (MQ=255)
                                  aTGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCaa                                                                                                  <  5:225644/135‑1 (MQ=255)
                                    gggAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGt                                                                                            <  2:181162/139‑1 (MQ=255)
                                    gggAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGt                                                                                            >  4:298126/1‑139 (MQ=255)
                                    gggAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACAGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTCATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGt                                                                                            >  3:366116/1‑139 (MQ=255)
                                       aGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGAt                                                                                                                   >  5:368786/1‑113 (MQ=255)
                                       aGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGAt                                                                                                                   <  6:368786/113‑1 (MQ=255)
                                       aGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTAt                                                                                         >  4:321984/1‑139 (MQ=255)
                                          aGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTcc                                                                                      >  5:205679/1‑139 (MQ=255)
                                            tCAGATCCTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTAGCCCCGGCATCCTGATCAATAAAAAA‑ACTTCCAGCGCTCACTCCCTGCAAGTCGAAGCTGTTATTcc                                                                                      >  7:18478/1‑137 (MQ=255)
                                                aaaCTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCga                                                                                    >  4:200430/1‑135 (MQ=255)
                                                      aaTCCCCGTTACGGGCGGCCATCAGACCGCGGGAATAGTTATGATGGGCGGGATGTACTACACACTGCATCCTGATAAATAAAAAACACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCtt                                                                            <  2:226324/138‑1 (MQ=255)
                                                      aaTCCCCGTTACGCTCTGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCAGCAAGTCAAAGCGGTTATTCCGAGAGATCttc                                                                           >  3:129355/1‑137 (MQ=255)
                                                       atccccGTTACGCTCGGCAATCTTACCGCGGGATTATTTATGATGGGCTGGTTGTACTACACCCTGAATCCTCATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGaa                                                                         <  2:325520/136‑1 (MQ=255)
                                                       atccccGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGaa                                                                         <  7:63782/136‑1 (MQ=255)
                                                       atccccGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGa                                                                          <  8:354718/135‑1 (MQ=255)
                                                              ttACGCTCGGCAATCTCACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGAGAAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTg                                                                  <  3:312378/139‑1 (MQ=255)
                                                                 cgcTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGAc                                                               <  8:283482/137‑1 (MQ=255)
                                                                      ggCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCa                                                          <  8:231051/139‑1 (MQ=255)
                                                                        cAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAAAAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGc                                                        <  1:17603/139‑1 (MQ=255)
                                                                           tcTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACa                                                              >  7:280283/2‑130 (MQ=255)
                                                                           tcTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACa                                                              <  8:280283/129‑1 (MQ=255)
                                                                                   cgGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGAc                                                                      <  6:289045/114‑1 (MQ=255)
                                                                                   cgGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGAc                                                                      >  5:289045/1‑114 (MQ=255)
                                                                                              atgatgGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGatt                                  >  5:339752/1‑137 (MQ=255)
                                                                                              atgatgGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGat                                   <  6:339752/138‑2 (MQ=255)
                                                                                                 atgGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGc                                                        >  7:251165/1‑114 (MQ=255)
                                                                                                 atgGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGc                                                        <  8:251165/114‑1 (MQ=255)
                                                                                                   ggccTGGATGAACTACACACTGAACCCTGAACAAAAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGc                             <  5:39393/136‑1 (MQ=255)
                                                                                                    ggCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCCCTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGc                             >  1:264036/1‑138 (MQ=255)
                                                                                                    ggCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATg                                                            <  1:124151/107‑1 (MQ=255)
                                                                                                    ggCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATg                                                            >  2:124151/1‑107 (MQ=255)
                                                                                                    ggCTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGc                             <  5:353921/138‑1 (MQ=255)
                                                                                                      cTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCg                                                       <  1:10521/110‑1 (MQ=255)
                                                                                                      cTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCg                                                       >  2:10521/1‑110 (MQ=255)
                                                                                                      cTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCaaa                          >  8:183244/1‑139 (MQ=255)
                                                                                                      cTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCaaa                          >  3:82367/1‑139 (MQ=255)
                                                                                                      cTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCaaa                          >  7:161734/1‑139 (MQ=255)
                                                                                                      cTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCa                            <  7:186874/137‑1 (MQ=255)
                                                                                                      cTGGATGTACTACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCa                            >  8:186874/1‑137 (MQ=255)
                                                                                                      cTGGATGTACTACACACTGAATCCTCATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCAGGCCTCGGATTCAGGCata                          >  2:43168/1‑137 (MQ=255)
                                                                                                              actACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACa                                                              >  1:241859/3‑95 (MQ=255)
                                                                                                              actACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACa                                                              <  2:241859/93‑1 (MQ=255)
                                                                                                              actACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGc                                                        >  4:99414/3‑101 (MQ=255)
                                                                                                              actACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAAtt                   >  3:161521/3‑138 (MQ=255)
                                                                                                              actACACACTGAATCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAAtt                   >  1:46174/3‑138 (MQ=255)
                                                                                                              actACACACTGAATCCTGATCAAAAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGATAGATCTTGAAGACGCTGGACATGCAGc                                                        <  3:99414/99‑1 (MQ=255)
                                                                                                                   acactgaaTCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGatat              >  2:106591/7‑138 (MQ=255)
                                                                                                                    cactgaaTCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATAtt             >  7:117982/6‑138 (MQ=255)
                                                                                                                     actgaaTCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGAc                                                                      >  1:243342/5‑80 (MQ=255)
                                                                                                                     actgaaTCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGAc                                                                      <  2:243342/76‑1 (MQ=255)
                                                                                                                     actgaaTCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCt                                        <  5:170601/106‑1 (MQ=255)
                                                                                                                     actgaaTCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCt                                        >  6:170601/5‑110 (MQ=255)
                                                                                                                     actgaaTCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCa           >  7:63471/5‑139 (MQ=255)
                                                                                                                     actgaaTCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCa           >  8:93126/5‑139 (MQ=255)
                                                                                                                       tgaaTCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGt                                                                                            >  1:199829/3‑56 (MQ=255)
                                                                                                                       tgaaTCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGt                                                                                            <  2:199829/54‑1 (MQ=255)
                                                                                                                       tgaaTCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGc                                              <  4:347700/100‑1 (MQ=255)
                                                                                                                       tgaaTCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGc                                              >  3:347700/3‑102 (MQ=255)
                                                                                                                       tgaaTCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGc                             <  4:301039/117‑1 (MQ=255)
                                                                                                                       tgaaTCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGc                             >  3:301039/3‑119 (MQ=255)
                                                                                                                       tgaaTCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTcgtt         >  7:144242/3‑136 (MQ=255)
                                                                                                                       tgaaTCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCatt         >  5:4309/3‑137 (MQ=255)
                                                                                                                       tgaaTCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCatt         >  5:146506/3‑137 (MQ=255)
                                                                                                                       tgaaTCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCatt         >  4:334315/3‑137 (MQ=255)
                                                                                                                       tgaaTCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCatt         >  5:314478/3‑137 (MQ=255)
                                                                                                                       tgaaTCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCatt         >  1:367217/3‑137 (MQ=255)
                                                                                                                       tgaaTCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCatt         >  7:105848/3‑137 (MQ=255)
                                                                                                                       tgaaTCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCatt         >  7:215133/3‑137 (MQ=255)
                                                                                                                         aaTCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAATTGTAATTGATATTCAtttt       >  8:78735/1‑139 (MQ=255)
                                                                                                                         aaTCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCAtttt       >  4:278703/1‑139 (MQ=255)
                                                                                                                         aaTCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCAtttt       >  3:212732/1‑139 (MQ=255)
                                                                                                                         aaTCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCAtttt       >  2:343577/1‑139 (MQ=255)
                                                                                                                         aaTCCTGATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCAtttt       >  6:360250/1‑139 (MQ=255)
                                                                                                                              tgATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCaa   >  8:296858/2‑138 (MQ=255)
                                                                                                                               gATCAATAAAAAA‑GCTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCaaa  >  6:74976/1‑138 (MQ=255)
                                                                                                                               gATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAATACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCaaa  >  3:341627/1‑138 (MQ=255)
                                                                                                                               gATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCaaa  >  6:356481/1‑138 (MQ=255)
                                                                                                                               gATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCaaa  >  4:97367/1‑138 (MQ=255)
                                                                                                                               gATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCaaa  >  8:316779/1‑138 (MQ=255)
                                                                                                                               gATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCaaa  >  8:260364/1‑138 (MQ=255)
                                                                                                                               gATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCaaa  >  1:243864/1‑138 (MQ=255)
                                                                                                                               gATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCaaa  >  6:319681/1‑138 (MQ=255)
                                                                                                                               gATCAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCaaa  >  3:69393/1‑138 (MQ=255)
                                                                                                                                  |                                                                                                                                       
ACTTTTGATCGAGCACCCTGATACAGTGACAGTAATGGGAGCAGTCAGAAACTTAATTCCCGTTACACTCGGCAACCTGACCGCGGGAATGGTCATGATGGGCTGGATGTATTACACGCTCAATCCCGATAAATAAAAAA‑ACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGACTCCGGCAAATGTAATTGATATTCACTTTTCAAA  >  CP006881/3723822‑3724086

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A