Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A25 F54 I1 R1 119 63.3 2848040 95.5% 2719878 127.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 3,724,467 T→C K120K (AAA→AAG)  U712_19200 ← SacPA operon antiterminator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068813,724,4670TC97.3% 231.8 / NA 75K120K (AAA→AAG) U712_19200SacPA operon antiterminator
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (38/35);  minor base A (0/2);  total (38/37)
Fisher's exact test for biased strand distribution p-value = 2.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGAAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCGTATTCTTTTTTATAGAGCGCTTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCAGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCCCCTTCCGCATAGCT  >  CP006881/3724332‑3724601
                                                                                                                                       |                                                                                                                                      
ctcCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCcttt                                                                                                                                     >  8:346240/1‑139 (MQ=255)
ctcCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCctt                                                                                                                                      >  7:203423/1‑138 (MQ=255)
  ccGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTATTTTTTATAGAGCGCCTTTa                                                                                                                                    >  7:98102/1‑138 (MQ=255)
         catcTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATgc                                                                                                                            <  1:246213/139‑1 (MQ=255)
          atcTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATgca                                                                                                                           <  2:325814/139‑1 (MQ=255)
                gCCGTATGGTTGTGTAGGGCAATATACCCCGCTTCTTCTTGAGGCTGAGACTCGCCCAAAGTCTCTTTTACATGTCCGATCGCCGACAGGCCGATCTCATATTCTTATTTATAGAGCGCCTTTATCTCATGCAGCAAt                                                                                                                      <  5:267550/138‑1 (MQ=255)
                    tATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTAtt                                                                                                                 >  6:106029/1‑139 (MQ=255)
                            tgAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGcac                                                                                                           >  8:51541/1‑137 (MQ=255)
                            tgAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGcac                                                                                                           >  2:242839/1‑137 (MQ=255)
                            tgAAGGGCAATATAACCCGCTTCATCTTCAGGCAGAAACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCATTTTATTCTGcac                                                                                                           >  2:273044/1‑137 (MQ=255)
                              aaGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTAt                                                                                                                  >  1:182788/1‑128 (MQ=255)
                              aaGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTAt                                                                                                                  <  2:182788/128‑1 (MQ=255)
                              aaGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAagc                                                                                                       <  7:346240/139‑1 (MQ=255)
                              aaGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAagc                                                                                                       <  3:202593/139‑1 (MQ=255)
                               aGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATgca                                                                                                                           >  1:139510/1‑118 (MQ=255)
                               aGGGCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATgca                                                                                                                           <  2:139510/118‑1 (MQ=255)
                                  gCAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGcc                                                                                                   <  7:322191/139‑1 (MQ=255)
                                   cAATATAACCCGCTTCATCTTCAGGCAGAGACCCGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGcc                                                                                                   >  6:111709/1‑138 (MQ=255)
                                   cAATATAACCCGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGcc                                                                                                   >  7:304739/1‑138 (MQ=255)
                                           cccGCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGaa                                                                                          >  3:338644/1‑139 (MQ=255)
                                              gCTTCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATcc                                                                                       >  2:126533/1‑139 (MQ=255)
                                                ttCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGGGCCTTTATGTCATGCAGCAATTTATTATGCACAAGCAGCCCATTTTGATTCCtt                                                                                     >  3:324881/1‑139 (MQ=255)
                                                ttCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCtt                                                                                     >  2:186322/1‑139 (MQ=255)
                                                ttCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCtt                                                                                     >  4:61544/1‑139 (MQ=255)
                                                ttCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCtt                                                                                     >  8:212208/1‑139 (MQ=255)
                                                ttCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCtt                                                                                     >  7:352945/1‑139 (MQ=255)
                                                ttCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCtt                                                                                     >  1:297856/1‑139 (MQ=255)
                                                ttCATCTTCAGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATATCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCtt                                                                                     >  8:129231/1‑139 (MQ=255)
                                                         aGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCa                                                                            <  7:212208/139‑1 (MQ=255)
                                                         aGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCa                                                                            <  7:58234/139‑1 (MQ=255)
                                                         aGGCAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCGCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGc                                                                             >  2:175384/1‑138 (MQ=255)
                                                          ggcagagACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCaa                                                                           <  1:96905/136‑1 (MQ=255)
                                                                  ctcGCCAAATGGCTCTTTTACATGTCCGATCGCCCACCGGCAGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATAGCAAATGACaaa                                                                   <  6:158735/137‑1 (MQ=255)
                                                                    cGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGa                                                                                           >  7:316104/1‑113 (MQ=255)
                                                                    cGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGa                                                                                           <  8:316104/113‑1 (MQ=255)
                                                                                 ttttACATGTCCGATCGGCCACAGGCCGATCTGATAATCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAgcg                                                      <  4:324881/137‑1 (MQ=255)
                                                                                 ttttACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGATCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGcgcg                                                    >  1:271281/1‑139 (MQ=255)
                                                                                 ttttACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAgcgc                                                     >  1:181742/1‑138 (MQ=255)
                                                                                 ttttACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAgcg                                                      <  5:111709/137‑1 (MQ=255)
                                                                                 ttttACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAgcg                                                      <  1:186322/137‑1 (MQ=255)
                                                                                    tACATGTCCGATCGCCCACAGGCCTATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGat                                                  <  4:251317/138‑1 (MQ=255)
                                                                                    tACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGat                                                  <  2:352390/138‑1 (MQ=255)
                                                                                         gTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAAt                                            >  7:260838/1‑139 (MQ=255)
                                                                                         gTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAAt                                            >  2:243795/1‑139 (MQ=255)
                                                                                           ccGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAAtgt                                          >  6:22288/1‑139 (MQ=255)
                                                                                           ccGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAAtgt                                          >  3:2187/1‑139 (MQ=255)
                                                                                           ccGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGACGGAAAGCGCGATATGAAtgt                                          >  4:310138/1‑139 (MQ=255)
                                                                                             gATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGa                                        <  4:338644/139‑1 (MQ=255)
                                                                                             gATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGa                                        <  4:15898/139‑1 (MQ=255)
                                                                                                   ccACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCt                                   >  5:117558/1‑138 (MQ=255)
                                                                                                             aTCTCATATTCTTTTTTATATAGAGCCTTTATCTCATGCAGCAATTTATTATGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGTTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGcgccg                        >  4:271235/1‑139 (MQ=255)
                                                                                                             aTCTCATATTCTTTTTTATAGATCGCCTTTATCTCATGCAGCAATTTATTCTGAACAAGCAGCCCATTTTGAATCATTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGcgccg                        >  6:130067/1‑139 (MQ=255)
                                                                                                             aTCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCATCCCATTTTGAATCCTTTCGCTCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGGTCAGCGGcgccg                        >  8:293939/1‑139 (MQ=255)
                                                                                                             aTCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAgcg                                                      >  1:194551/1‑109 (MQ=255)
                                                                                                             aTCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGcgccg                        >  2:196117/1‑139 (MQ=255)
                                                                                                             aTCTCATATTCTTTTTTATAGAGCGCCTTTATATCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAgcg                                                      <  2:194551/109‑1 (MQ=255)
                                                                                                                  atATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTTAATGCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATAGCTCAGCGGCGCcgcg                      <  4:147191/135‑1 (MQ=255)
                                                                                                                  atATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCcgcg                      <  6:58864/135‑1 (MQ=255)
                                                                                                                  atATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGc                   <  1:175384/138‑1 (MQ=255)
                                                                                                                  atATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGGCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCcgcg                      <  5:22288/135‑1 (MQ=255)
                                                                                                                  atATTCTTTTTTATAGAGCGCCATTATCTCATGCAGAAATTTATTCAGCACAAGCAGCACATTTTGAATCCTTTCGATCGCAACGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGc                   <  5:108343/138‑1 (MQ=255)
                                                                                                                   tATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCTATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCGCAGCGGCGCCGCGAGCt                  <  6:108241/139‑1 (MQ=255)
                                                                                                                   tATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAgcg                                                      >  1:168935/1‑103 (MQ=255)
                                                                                                                   tATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAgcg                                                      <  2:168935/103‑1 (MQ=255)
                                                                                                                   tATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCt                  >  5:153776/1‑139 (MQ=255)
                                                                                                                        ttttttATAGAGCTCCTTTATCTCATGCAGCAATTTATTCTGCACAATCAGCCCATATTGAATCCTTTCGATCTCAAAGGACAAATGTTCTGAAAGCGATATATGAATGTGATCGATCAGCGGCGCCGCGAGcactcc              >  6:199450/1‑133 (MQ=255)
                                                                                                                          ttttATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTctcc              <  5:106029/136‑1 (MQ=255)
                                                                                                                             tataGAGCGCATTTATCTCATGCAGCAATTTATTCTGAACAAGCAGCCAATTTTGAATCCTTTCGATCTCAAAGGACAAATGGTCGGAAAGCGCGATATGAATTTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCg         <  5:199450/138‑1 (MQ=255)
                                                                                                                               taGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCa       <  4:2187/138‑1 (MQ=255)
                                                                                                                               taGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCa       <  1:126533/138‑1 (MQ=255)
                                                                                                                               taGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGGCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCAt      <  3:348903/139‑1 (MQ=255)
                                                                                                                               taGAGCGCATTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCACATTTTGAATCCTTTCTATCGAAAATGACAAATGGTCGGAAAGCGCTATATGAATGTTATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCa       <  3:271235/138‑1 (MQ=255)
                                                                                                                                  agCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGc   <  6:117558/139‑1 (MQ=255)
                                                                                                                                  agCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGc   <  1:243795/139‑1 (MQ=255)
                                                                                                                                  agCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGc   <  8:304739/139‑1 (MQ=255)
                                                                                                                                    cgcCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCt  >  4:134483/1‑138 (MQ=255)
                                                                                                                                    cgcCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCt  >  1:50303/1‑138 (MQ=255)
                                                                                                                                       |                                                                                                                                      
CTCCGCATCCATCTTCGCCGTATGGATGTGAAGGGCAATATAACCCGCTTCATCTTCAGGAAGAGACACGCCCAATGTCTCTTTTACATGTCCGATCGCCCACAGGCCGATCTCGTATTCTTTTTTATAGAGCGCTTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCAGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCCCCTTCCGCATAGCT  >  CP006881/3724332‑3724601

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A