Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A25 F54 I1 R1 119 63.3 2848040 95.5% 2719878 127.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 3,724,542 A→G S95S (TCT→TCC)  U712_19200 ← SacPA operon antiterminator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068813,724,5420AG97.7% 312.4 / NA 87S95S (TCT→TCC) U712_19200SacPA operon antiterminator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (37/48);  minor base T (2/0);  total (39/48)
Fisher's exact test for biased strand distribution p-value = 1.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTTACATGTCCGATCGCCCACAGGCCGATCTCGTATTCTTTTTTATAGAGCGCTTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCAGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCCCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCAATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTAAA  >  CP006881/3724413‑3724661
                                                                                                                                 |                                                                                                                       
ttttACATGTCCGATCGGCCACAGGCCGATCTGATAATCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAgcg                                                                                                                  <  4:324881/137‑1 (MQ=255)
ttttACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGATCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGcgcg                                                                                                                >  1:271281/1‑139 (MQ=255)
ttttACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAgcgc                                                                                                                 >  1:181742/1‑138 (MQ=255)
ttttACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAgcg                                                                                                                  <  5:111709/137‑1 (MQ=255)
ttttACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAgcg                                                                                                                  <  1:186322/137‑1 (MQ=255)
   tACATGTCCGATCGCCCACAGGCCTATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGat                                                                                                              <  4:251317/138‑1 (MQ=255)
   tACATGTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGat                                                                                                              <  2:352390/138‑1 (MQ=255)
        gTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAAt                                                                                                        >  2:243795/1‑139 (MQ=255)
        gTCCGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAAt                                                                                                        >  7:260838/1‑139 (MQ=255)
          ccGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAAtgt                                                                                                      >  3:2187/1‑139 (MQ=255)
          ccGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAAtgt                                                                                                      >  6:22288/1‑139 (MQ=255)
          ccGATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGACGGAAAGCGCGATATGAAtgt                                                                                                      >  4:310138/1‑139 (MQ=255)
            gATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGa                                                                                                    <  4:15898/139‑1 (MQ=255)
            gATCGCCCACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGa                                                                                                    <  4:338644/139‑1 (MQ=255)
                  ccACAGGCCGATCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCt                                                                                               >  5:117558/1‑138 (MQ=255)
                            aTCTCATATTCTTTTTTATATAGAGCCTTTATCTCATGCAGCAATTTATTATGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGTTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGcgccg                                                                                    >  4:271235/1‑139 (MQ=255)
                            aTCTCATATTCTTTTTTATAGATCGCCTTTATCTCATGCAGCAATTTATTCTGAACAAGCAGCCCATTTTGAATCATTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGcgccg                                                                                    >  6:130067/1‑139 (MQ=255)
                            aTCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCATCCCATTTTGAATCCTTTCGCTCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGGTCAGCGGcgccg                                                                                    >  8:293939/1‑139 (MQ=255)
                            aTCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAgcg                                                                                                                  >  1:194551/1‑109 (MQ=255)
                            aTCTCATATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGcgccg                                                                                    >  2:196117/1‑139 (MQ=255)
                            aTCTCATATTCTTTTTTATAGAGCGCCTTTATATCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAgcg                                                                                                                  <  2:194551/109‑1 (MQ=255)
                                 atATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTTAATGCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATAGCTCAGCGGCGCcgcg                                                                                  <  4:147191/135‑1 (MQ=255)
                                 atATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCcgcg                                                                                  <  6:58864/135‑1 (MQ=255)
                                 atATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGc                                                                               <  1:175384/138‑1 (MQ=255)
                                 atATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGGCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCcgcg                                                                                  <  5:22288/135‑1 (MQ=255)
                                 atATTCTTTTTTATAGAGCGCCATTATCTCATGCAGAAATTTATTCAGCACAAGCAGCACATTTTGAATCCTTTCGATCGCAACGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGc                                                                               <  5:108343/138‑1 (MQ=255)
                                  tATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCTATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCGCAGCGGCGCCGCGAGCt                                                                              <  6:108241/139‑1 (MQ=255)
                                  tATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAgcg                                                                                                                  >  1:168935/1‑103 (MQ=255)
                                  tATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAgcg                                                                                                                  <  2:168935/103‑1 (MQ=255)
                                  tATTCTTTTTTATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCt                                                                              >  5:153776/1‑139 (MQ=255)
                                       ttttttATAGAGCTCCTTTATCTCATGCAGCAATTTATTCTGCACAATCAGCCCATATTGAATCCTTTCGATCTCAAAGGACAAATGTTCTGAAAGCGATATATGAATGTGATCGATCAGCGGCGCCGCGAGcactcc                                                                          >  6:199450/1‑133 (MQ=255)
                                         ttttATAGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTctcc                                                                          <  5:106029/136‑1 (MQ=255)
                                            tataGAGCGCATTTATCTCATGCAGCAATTTATTCTGAACAAGCAGCCAATTTTGAATCCTTTCGATCTCAAAGGACAAATGGTCGGAAAGCGCGATATGAATTTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCg                                                                     <  5:199450/138‑1 (MQ=255)
                                              taGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCa                                                                   <  4:2187/138‑1 (MQ=255)
                                              taGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCa                                                                   <  1:126533/138‑1 (MQ=255)
                                              taGAGCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGGCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCAt                                                                  <  3:348903/139‑1 (MQ=255)
                                              taGAGCGCATTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCACATTTTGAATCCTTTCTATCGAAAATGACAAATGGTCGGAAAGCGCTATATGAATGTTATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCa                                                                   <  3:271235/138‑1 (MQ=255)
                                                 agCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGc                                                               <  6:117558/139‑1 (MQ=255)
                                                 agCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGc                                                               <  1:243795/139‑1 (MQ=255)
                                                 agCGCCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGc                                                               <  8:304739/139‑1 (MQ=255)
                                                   cgcCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCt                                                              >  4:134483/1‑138 (MQ=255)
                                                   cgcCTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCt                                                              >  1:50303/1‑138 (MQ=255)
                                                         tATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCtgatga                                                         <  2:297856/137‑1 (MQ=255)
                                                                tGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCCGCGTCGCCGCGAGCTCTCCTTCCGCAAAGATTATGATATACt                                                   >  7:289395/1‑136 (MQ=255)
                                                                tGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCtt                                                                        <  2:64770/115‑1 (MQ=255)
                                                                tGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCtt                                                                        >  5:345133/1‑115 (MQ=255)
                                                                tGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCtt                                                                        <  6:345133/115‑1 (MQ=255)
                                                                tGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCtt                                                                        >  1:64770/1‑115 (MQ=255)
                                                                tGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCa                                                                   >  4:156253/1‑120 (MQ=255)
                                                                tGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCa                                                                   <  3:156253/120‑1 (MQ=255)
                                                                tGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAg                                                >  6:139307/1‑139 (MQ=255)
                                                                tGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAg                                                >  4:112714/1‑139 (MQ=255)
                                                                tGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAg                                                >  6:135514/1‑139 (MQ=255)
                                                                tGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAg                                                <  7:51541/139‑1 (MQ=255)
                                                                tGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAg                                                >  7:112457/1‑139 (MQ=255)
                                                                tGCAGCAATTTATTCTGCACAAGCAGCACATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAg                                                <  1:108166/139‑1 (MQ=255)
                                                                tGCAGCAATTTATTCTGCACAAGCAGCACATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAg                                                >  2:108166/1‑139 (MQ=255)
                                                                  cagcaATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGc                                               <  6:153776/138‑1 (MQ=255)
                                                                   agcaATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTa                                             <  5:135514/139‑1 (MQ=255)
                                                                   agcaATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTa                                             <  1:196117/139‑1 (MQ=255)
                                                                    gcaATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTAt                                            <  8:112457/139‑1 (MQ=255)
                                                                     caATTTATTCTGCACAAGCAGCCCATTTTGATTCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGTGCCGCGAGCTCTCCTTCCGCa                                                                   >  3:101915/1‑115 (MQ=255)
                                                                     caATTTATTCTGCACAAGCAGCCCATTTTGATTCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCa                                                                   <  4:101915/115‑1 (MQ=255)
                                                                          tATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCtgatg                                                          <  1:43010/119‑1 (MQ=255)
                                                                          tATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCtgatg                                                          >  2:43010/1‑119 (MQ=255)
                                                                                 cacaAGCAGCCTATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGAGCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGc                                  <  2:271281/136‑1 (MQ=255)
                                                                                 cacaAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAgcg                                                                                                                  <  4:331985/56‑1 (MQ=255)
                                                                                 cacaAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAgcg                                                                                                                  >  3:331985/1‑56 (MQ=255)
                                                                                 cacaAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCtgatg                                                          >  8:207058/1‑112 (MQ=255)
                                                                                 cacaAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCtgatg                                                          <  7:207058/112‑1 (MQ=255)
                                                                                 cacaAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGctc                                <  8:98102/138‑1 (MQ=255)
                                                                                 cacaAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGctc                                <  7:177347/138‑1 (MQ=255)
                                                                                 cacaAGAAGCCCATTTTGAATCCTTTCGATCGGAAAGGACAAATGGTCTGATAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCCAGATCTCCTTCCGCATAGCTGATGATAACCTCAGCTATATCTATATGctca                               >  6:146948/1‑138 (MQ=255)
                                                                                      gcagcCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCgg                          >  1:276956/1‑139 (MQ=255)
                                                                                         gcCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAg                       <  3:310138/139‑1 (MQ=255)
                                                                                         gcCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAg                       <  8:352945/139‑1 (MQ=255)
                                                                                         gcCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCGCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAg                       >  3:75761/1‑139 (MQ=255)
                                                                                              ttttGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCa                        >  2:143873/1‑133 (MQ=255)
                                                                                              ttttGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCa                        <  1:143873/133‑1 (MQ=255)
                                                                                              ttttGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGttt                  >  4:47430/1‑139 (MQ=255)
                                                                                                ttGAATCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGGTATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTg                 <  2:41986/138‑1 (MQ=255)
                                                                                                    aTCCTTTCGATCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCGCTCCTGCCGCATAGAAGAAGATATACTCAGCGATTGATTAATGCTCCTCCGGCACTGGTTGCAgg             >  2:303855/1‑138 (MQ=255)
                                                                                                         ttCGATCGCAAAGGACAAATGGTCGGGAAGCGCGATATGAATGTGATCGCTCCGCGGCGCCGCGCGCTCTCCGTCCGCATAGCTGATGATCTCCTCAGCTATTACTATATGCTCCTCCGGCAGGGGTTGCAGGAttttt       >  3:189004/1‑137 (MQ=255)
                                                                                                             aTCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGtttga   <  7:129231/139‑3 (MQ=255)
                                                                                                              tCGCAAAGGACAAATGGTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTgaa  <  8:203423/139‑1 (MQ=255)
                                                                                                                              gTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTa                                             >  1:265600/1‑80 (MQ=255)
                                                                                                                              gTCGGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCTCCTTCCGCATAGCTGATGATATCCTCAGCTa                                             <  2:265600/80‑1 (MQ=255)
                                                                                                                                 |                                                                                                                       
TTTTACATGTCCGATCGCCCACAGGCCGATCTCGTATTCTTTTTTATAGAGCGCTTTTATCTCATGCAGCAATTTATTCTGCACAAGCAGCCCATTTTGAATCCTTTCGATCGCAAAGGACAAATGGTCAGAAAGCGCGATATGAATGTGATCGCTCAGCGGCGCCGCGAGCTCCCCTTCCGCATAGCTGATGATATCCTCAGCTATTTCAATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTAAA  >  CP006881/3724413‑3724661

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A