Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A12 F63 I0 R1 227 97.7 3613754 96.1% 3472817 135.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,177,586 T→G 81.8% L770R (CTC→CGC)  rpoC → RNA polymerase, beta prime subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,177,5860TG81.8% 205.2 / 28.6 102L770R (CTC→CGC) rpoCRNA polymerase, beta prime subunit
Reads supporting (aligned to +/- strand):  ref base T (8/8);  new base G (36/39);  total (49/53)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 102 total aligned reads displayed.

ATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGA  >  CP009273/4177450‑4177715
                                                                                                                                        |                                                                                                                                 
atgGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACGCCa                                                                                                                                >  1:1027871/1‑140 (MQ=255)
atgGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACGCCa                                                                                                                                >  1:870610/1‑140 (MQ=255)
atgGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACGCCa                                                                                                                                >  1:775079/1‑140 (MQ=255)
atgGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACGCCa                                                                                                                                >  1:1526957/1‑140 (MQ=255)
atgGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACGCCa                                                                                                                                >  1:620589/1‑140 (MQ=255)
atgGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACGCCa                                                                                                                                >  1:1163735/1‑140 (MQ=255)
atgGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACGCCa                                                                                                                                >  1:609342/1‑140 (MQ=255)
atgGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACGCCa                                                                                                                                >  1:578806/1‑140 (MQ=255)
atgGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACCCCa                                                                                                                                >  1:767217/1‑140 (MQ=255)
 tgGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACGCCAg                                                                                                                               <  2:1372676/140‑1 (MQ=255)
    ccGACTCGGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACCCCAGTAc                                                                                                                            <  2:688539/140‑1 (MQ=255)
     cGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACt                                                                                                                           <  2:328038/140‑1 (MQ=255)
     cGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACt                                                                                                                           <  2:1082374/140‑1 (MQ=255)
       aCTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACGCCAGTACTTc                                                                                                                         >  1:1091621/1‑140 (MQ=255)
       aCTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACGCCAGTACTTc                                                                                                                         >  1:1699932/1‑140 (MQ=255)
          ccGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACCCCAGTACTTCAtc                                                                                                                      <  2:379003/140‑1 (MQ=255)
             gTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATGGAATCGCCAATCACCGCGATCTTGCGTGAAGGTCTGAACGTACGCCAGTAGTTCATCTcc                                                                                                                   >  2:749988/1‑140 (MQ=255)
              tGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACCCCAGTACTTCATCTcca                                                                                                                  >  1:27030/1‑140 (MQ=255)
                  cgTGGTTCTGCGGCAGTGTTTCGTCAGGTTGCTGGTATGCGTGGTCTGATGGCGAAGGCGGTTGTGTCGCTGATCGAAACGCCAAGCACGGCGAACTACGGTGTAGGTCTGGACGTACCGCAGTACTTCTTCTccaccct                                                                                                              >  2:855153/1‑139 (MQ=255)
                  cgTGGTTCTGCGGCACAGATTCGTCAGCTTGGTGGGATGCGGGGTCTGACGGCGAAGCCGGATGGCTCGATCATCGAAAGGCCCAGCACCGCGAACGTCCGGGAGGGTCTGAACGTACGCCGGGACTTCATCTcctccca                                                                                                              >  2:855156/1‑140 (MQ=255)
                  cgTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACGCCAGTACTTCATCTccaccca                                                                                                              >  2:1143832/1‑140 (MQ=255)
                  cgTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACGCCAGTACTTCATCTccaccca                                                                                                              <  2:1700683/140‑1 (MQ=255)
                     ggTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGACGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGGCCGTACGCCAGGACTTCATCTCCACGCACgg                                                                                                           >  1:916126/1‑140 (MQ=255)
                     ggTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACGCCAGTACTTCATCTCCACCCACgg                                                                                                           >  1:1037528/1‑140 (MQ=255)
                       ttCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACGCCAGTACTTCATCTCCACCCACGGTg                                                                                                         <  1:1448783/140‑1 (MQ=255)
                       cccagCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTg                                                                                                         >  1:338657/5‑140 (MQ=255)
                       cccagCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTg                                                                                                         >  1:72973/5‑140 (MQ=255)
                             ggCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACCCCAGTACTTCATCTCCACCCACGGTGCTCGTa                                                                                                   <  2:767217/140‑1 (MQ=255)
                                acaGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACGCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAg                                                                                                <  2:1699932/140‑1 (MQ=255)
                                 caGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCTTGAAGGTCTTAACGTACCCCAGTACTTCAACACCACCCACGGTGCTCGTAAAgt                                                                                               >  1:1254190/1‑139 (MQ=255)
                                 caGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACGCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAgg                                                                                               >  1:538180/1‑140 (MQ=255)
                                 caGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACGCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAgg                                                                                               >  2:1206294/1‑140 (MQ=255)
                                 caGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACGCCAGTACTTCATCTCCACCCACGCTGCTCGTAAAgg                                                                                               >  1:538223/1‑140 (MQ=255)
                                 caGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACCCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAgg                                                                                               >  1:614617/1‑140 (MQ=255)
                                  aGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACGCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGt                                                                                              <  2:60971/140‑1 (MQ=255)
                                  aGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACGCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGt                                                                                              <  1:121935/140‑1 (MQ=255)
                                  aGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACGCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGt                                                                                              <  2:108215/140‑1 (MQ=255)
                                    aTTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACGCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCt                                                                                            >  2:547591/1‑140 (MQ=255)
                                    aTTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACGCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCt                                                                                            >  2:1662984/1‑140 (MQ=255)
                                    aTTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACGCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGGCt                                                                                            >  2:1663059/1‑140 (MQ=255)
                                         tCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACGCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCgg                                                                                       >  1:1069446/1‑140 (MQ=255)
                                         tCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACGCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCgg                                                                                       >  1:361413/1‑140 (MQ=255)
                                          cAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACGCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGa                                                                                      <  2:1202791/140‑1 (MQ=255)
                                          cAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACGCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGa                                                                                      <  2:1184644/140‑1 (MQ=255)
                                            gCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACGTCCGTGCAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATa                                                                                    >  2:836618/1‑140 (MQ=255)
                                              ttGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATAcc                                                                                  <  1:1146174/140‑1 (MQ=255)
                                              ttGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATAcc                                                                                  <  1:1146300/140‑1 (MQ=255)
                                              ttGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACGCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATAcc                                                                                  <  2:225381/140‑1 (MQ=255)
                                              ttGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACGCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATAcc                                                                                  <  1:1461561/140‑1 (MQ=255)
                                                gCTGGTATGCGTGGTCTGATGGTGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACGCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAg                                                                                                >  1:1171471/1‑124 (MQ=255)
                                                gCTGGTATGCGTGGTCTGATGGTGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACGCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAg                                                                                                <  2:1171471/124‑1 (MQ=255)
                                                     tATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGACGGTCTGAACGTACGCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGa                                                                           >  2:483116/1‑140 (MQ=255)
                                                     tATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACGCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGa                                                                           >  2:695167/1‑140 (MQ=255)
                                                     tATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACGCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGa                                                                           >  2:1337161/1‑140 (MQ=255)
                                                                  aTGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACGCCAGTACtt                                                                                                                          >  1:1561195/1‑80 (MQ=255)
                                                                  aTGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACGCCAGTACtt                                                                                                                          <  2:1561195/80‑1 (MQ=255)
                                                                  aTGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACGCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTc                                                              >  2:457408/1‑140 (MQ=255)
                                                                  aTGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACGCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTc                                                              >  2:647646/1‑140 (MQ=255)
                                                                  aTGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACGCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTc                                                              >  2:1755093/1‑140 (MQ=255)
                                                                                tGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACtc                                                <  1:626128/140‑1 (MQ=255)
                                                                                 ggCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACGCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACtcg                                               <  1:1739170/140‑1 (MQ=255)
                                                                                     ccATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACGCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTcgtcgt                                           <  2:579137/140‑1 (MQ=255)
                                                                                     ccATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACGCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTcgtcgt                                           <  1:127980/140‑1 (MQ=255)
                                                                                     ccATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACGCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTcgtcgt                                           <  1:41407/140‑1 (MQ=255)
                                                                                        tcatcGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTg                                        >  2:497718/1‑140 (MQ=255)
                                                                                        tcatcGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTg                                        >  2:1638468/1‑140 (MQ=255)
                                                                                        tcatcGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTg                                        >  1:898322/1‑140 (MQ=255)
                                                                                        tcagcGAAACCCCAATCCCCGCGAACTTCCGTGAAGGTCTGAACGGACGCCAGTACTTCATCGCCACCCACGGGGCTCGTCAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTg                                        >  2:723292/1‑140 (MQ=255)
                                                                                         catcGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACGCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTgg                                       <  2:560184/140‑1 (MQ=255)
                                                                                               aaCGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACGCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACg                                 <  1:175350/140‑1 (MQ=255)
                                                                                               aaCGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACGCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACg                                 <  1:902216/140‑1 (MQ=255)
                                                                                                aCGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGt                                <  2:465029/140‑1 (MQ=255)
                                                                                                aCGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGt                                <  2:818861/140‑1 (MQ=255)
                                                                                                aCGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCGGACTCGTCGTCTGGTTGACGt                                <  2:1764492/140‑1 (MQ=255)
                                                                                                aCGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACGCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGt                                <  2:609342/140‑1 (MQ=255)
                                                                                                aCGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACGCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGt                                <  2:775079/140‑1 (MQ=255)
                                                                                                aCGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACGCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGt                                <  2:1027871/140‑1 (MQ=255)
                                                                                                      aTCAGCGCGAACTTCCGTGAAGGTCTGAACGTACCCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCa                          <  2:1254190/140‑1 (MQ=255)
                                                                                                      aTCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCa                          >  1:452712/1‑140 (MQ=255)
                                                                                                      aTCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCa                          >  1:393885/1‑140 (MQ=255)
                                                                                                      aTCACCGCGAACTTCCGTGAAGGTCTGAACGTACGCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAACCTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCa                          <  1:1725278/140‑1 (MQ=255)
                                                                                                      aTCACCGCGAACTTCCGTGAAGGTCTGAACGTACGCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCa                          <  2:620589/140‑1 (MQ=255)
                                                                                                      aTCACCGCGAACTTCCGTGAAGGTCTGAACGTACGCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCa                          <  2:1526957/140‑1 (MQ=255)
                                                                                                      aTCACCGCGAACTTCCGTGAAGGTCTGAACGTACGCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCa                          <  2:1163735/140‑1 (MQ=255)
                                                                                                      aTCACCGCGAACTTCCGTGAAGGTCTGAACGTACGCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCa                          <  2:578806/140‑1 (MQ=255)
                                                                                                      aTCACCGCGAACTTCCGTGAAGGTCTGAACGTACGCCAGTACTTCATCTCCACCCACGGTGCTAGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCa                          <  1:342375/140‑1 (MQ=255)
                                                                                                      aTCACCGCGAACTTCCGTGAAGGTCTGAACGTACCCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCa                          <  2:614617/140‑1 (MQ=255)
                                                                                                            gcgAACTTCCGTGAAGGTCTGAACGTACGCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTc                                                              >  2:211783/1‑98 (MQ=255)
                                                                                                            gcgAACTTCCGTGAAGGTCTGAACGTACGCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTc                                                              <  1:211783/98‑1 (MQ=255)
                                                                                                                  ttCCGTTTAGGTCTGAACGTACGCCAGTACTTCATCTCCACCGATGGTGCTCGTAAAGGTCTTGCGGATACCGCACGGAAAACTGCGAACTCCGGTTACCTGTCTCGTCGTCTGGTTGACGTGGCGCAGGACCTggtggt              <  1:435222/140‑1 (MQ=255)
                                                                                                                  ttCCGTGAAGGTCTGAACGTACGCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTggtggt              <  1:481662/140‑1 (MQ=255)
                                                                                                                  ttCCGTGAAGGTCTGAACGTACGCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTggtggt              <  1:481664/140‑1 (MQ=255)
                                                                                                                         aaGGTCTGAACGTACGCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGaa       <  1:233249/140‑1 (MQ=255)
                                                                                                                            gTCTGAACGTACGCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGAc    >  1:1478271/1‑140 (MQ=255)
                                                                                                                            gTCTGAACGTACGCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGAc    >  1:640408/1‑140 (MQ=255)
                                                                                                                            gTCTGAACGTACCCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGAc    <  2:1367991/140‑1 (MQ=255)
                                                                                                                              cttaaCGTACGCCAGTACTTCATCTCCACACACGGTGCTCGTAAAGGTCTGGCGGAAACAGCACTCAAAACTGCGAACTCCGGTTACCAGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGa  >  2:1587277/4‑140 (MQ=255)
                                                                                                                              cTGAACGTACGCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGa  <  1:457408/140‑1 (MQ=255)
                                                                                                                              cTGAACGTACGCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGa  <  1:1755093/140‑1 (MQ=255)
                                                                                                                              cTGAACGTACGCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGa  <  1:647646/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                 
ATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGA  >  CP009273/4177450‑4177715

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A